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    Phytase variants
    16.
    发明授权
    Phytase variants 有权
    植酸酶变体

    公开(公告)号：US09451783B2

    公开(公告)日：2016-09-27

    申请号：US14504517

    申请日：2014-10-02

    Applicant: Novozymes A/S

    Inventor： Leonardo De Maria ,  Carsten Andersen ,  Lars Kobberoee Skov ,  Mikael Blom Soerensen

    IPC: C12N9/16 ,  A23K1/165

    CPC classification number: C12N9/16 ,  A23K20/189 ,  C12Y301/03

    Abstract: The present invention relates to a phytase which has at least 74% identity to a phytase derived from Citrobacter braakii and comprises at least one alteration as compared to this phytase. These phytase variants have amended, preferably improved, properties, such as thermostability, temperature profile, pH profile, specific activity, performance in animal feed, reduced protease sensitiliby, and/or an amended glycosylation pattern. The invention also relates to DNA encoding these phytases, methods of their production, as well as the use thereof, e.g., in animal feed and animal feed additives.

    Abstract translation: 本发明涉及一种植酸酶，其与来自柠檬酸杆菌的植酸酶具有至少74％的同一性，并且与该植酸酶相比包含至少一个改变。 这些植酸酶变体具有修饰，优选改进的性质，例如热稳定性，温度曲线，pH曲线，比活性，动物饲料中的性能，降低的蛋白酶敏感性和/或修饰的糖基化模式。 本发明还涉及编码这些植酸酶的DNA，其生产方法及其用途，例如在动物饲料和动物饲料添加剂中。

    Phytase variants
    17.
    发明授权
    Phytase variants 有权
    植酸酶变体

    公开(公告)号：US08877471B2

    公开(公告)日：2014-11-04

    申请号：US13874954

    申请日：2013-05-01

    Applicant: Novozymes A/S

    Inventor： Leonardo De Maria ,  Carsten Andersen ,  Lars Kobberoee Skov ,  Mikael Blom Soerensen

    IPC: C12N9/00 ,  C12N9/16 ,  A23K1/165

    CPC classification number: C12N9/16 ,  A23K20/189 ,  C12Y301/03

    Abstract: The present invention relates to a phytase which has at least 74% identity to a phytase derived from Citrobacter braakii and comprises at least one alteration as compared to this phytase. These phytase variants have amended, preferably improved, properties, such as thermostability, temperature profile, pH profile, specific activity, performance in animal feed, reduced protease sensitiliby, and/or an amended glycosylation pattern. The invention also relates to DNA encoding these phytases, methods of their production, as well as the use thereof, e.g. in animal feed and animal feed additives.

    Abstract translation: 本发明涉及一种植酸酶，其与来自柠檬酸杆菌的植酸酶具有至少74％的同一性，并且与该植酸酶相比包含至少一个改变。 这些植酸酶变体具有修饰，优选改进的性质，例如热稳定性，温度曲线，pH曲线，比活性，动物饲料中的性能，降低的蛋白酶敏感性和/或修饰的糖基化模式。 本发明还涉及编码这些植酸酶的DNA，其生产方法及其用途，例如， 在动物饲料和动物饲料添加剂中。

    Protease variants
    18.
    发明授权
    Protease variants 有权
    蛋白酶变体

    公开(公告)号：US08772011B2

    公开(公告)日：2014-07-08

    申请号：US13738490

    申请日：2013-01-10

    Applicant: Novozymes A/S

    Inventor： Leonardo De Maria ,  Carsten Andersen ,  Lars Lehmann Hylling Christensen ,  Soren Flensted Lassen ,  Peter Rahbek Ostergaard

    IPC: C12N9/52 ,  A23K1/165

    CPC classification number: C12N9/58 ,  A01K2217/05 ,  A23K20/189 ,  C12N9/48 ,  C12N9/52 ,  C12N2310/111

    Abstract: The invention relates to a novel 3D structure encoding a Nocardiopsis protease, as well as to variants of parent protease homologous to Nocardiopsis proteases, preferably of improved thermostability and/or with an amended temperature activity profile. The invention also relates to DNA sequences encoding such variants, their production in a recombinant host cell, as well as methods of using the variants, in particular within the field of animal feed and detergents. The invention furthermore relates to methods of generating and preparing protease variants of amended properties.

    Abstract translation: 本发明涉及编码诺卡氏菌蛋白酶的新型3D结构，以及与诺卡氏菌蛋白酶同源的亲本蛋白酶的变体，优选具有改善的热稳定性和/或具有修改的温度活性分布。 本发明还涉及编码这些变体的DNA序列，其在重组宿主细胞中的产生，以及使用该变体，特别是在动物饲料和洗涤剂领域中的方法。 本发明还涉及生成和制备修饰性质的蛋白酶变体的方法。

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