公开(公告)号：US10890585B2

公开(公告)日：2021-01-12

申请号：US15186581

申请日：2016-06-20

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Hans-Peter Josel ,  Dieter Heindl ,  Thomas Froehlich ,  Stefanie Froehner

IPC: C12N11/06 ,  C12N11/08 ,  G01N33/569 ,  G01N33/50 ,  G01N33/543 ,  C12N5/00 ,  C12N11/14

Abstract: The present invention relates to a method of immobilizing a cell on a support, the method comprising a) providing a compound or salt thereof comprising, preferably consisting of, one or more hydrophobic domains attached to a hydrophilic domain, wherein the one or more hydrophobic domains are covalently bound to said hydrophilic domain, and wherein the one or more hydrophobic domains each comprise a linear lipid, a steroid or a hydrophobic vitamin, and wherein the hydrophilic domain comprises a polyethylene glycol (PEG) moiety, and wherein the compound comprises a linking group; b) contacting a cell with the compound under conditions allowing the interaction of the compound with the membrane of the cell, thereby immobilizing the linking group on the surface of the cell; and c) contacting the linking group immobilized on the cell with a support capable of binding the linking group, thereby immobilizing the cell on the support.

公开(公告)号：US20170363624A1

公开(公告)日：2017-12-21

申请号：US15186581

申请日：2016-06-20

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Hans-Peter Josel ,  Dieter Heindl ,  Thomas Froehlich ,  Stefanie Froehner

IPC: G01N33/569 ,  C12N11/08 ,  C12N11/06

CPC classification number: G01N33/56972 ,  C12N5/0068 ,  C12N11/06 ,  C12N11/08 ,  C12N11/14 ,  C12N2503/00 ,  C12N2533/20 ,  C12N2533/30 ,  G01N33/5005 ,  G01N33/54353 ,  G01N33/56966

Abstract: The present invention relates to a method of immobilizing a cell on a support, the method comprising a) providing a compound or salt thereof comprising, preferably consisting of, one or more hydrophobic domains attached to a hydrophilic domain, wherein the one or more hydrophobic domains are covalently bound to said hydrophilic domain, and wherein the one or more hydrophobic domains each comprise a linear lipid, a steroid or a hydrophobic vitamin, and wherein the hydrophilic domain comprises a polyethylene glycol (PEG) moiety, and wherein the compound comprises a linking group; b) contacting a cell with the compound under conditions allowing the interaction of the compound with the membrane of the cell, thereby immobilizing the linking group on the surface of the cell; and c) contacting the linking group immobilized on the cell with a support capable of binding the linking group, thereby immobilizing the cell on the support.

公开(公告)号：US20160145281A1

公开(公告)日：2016-05-26

申请号：US14867074

申请日：2015-09-28

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Robert Cysewski ,  Luisa de Cola ,  Jesus Miguel Fernandez Hernandez ,  Hans-Peter Josel ,  Eloisa Lopez-Calle ,  Toralf Zarnt

IPC: C07F15/00 ,  G01N21/76 ,  G01N33/58

CPC classification number: C07F15/0033 ,  G01N21/76 ,  G01N33/533 ,  G01N33/582 ,  G01N2458/30

Abstract: Novel iridium-based Ir(III) luminescent complexes, conjugates comprising these complexes as a label and their application, for example in the electrochemiluminescence based detection of an analyte.

公开(公告)号：US20150147750A1

公开(公告)日：2015-05-28

申请号：US14609552

申请日：2015-01-30

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Frank Bergmann ,  Robert Cysewski ,  Luisa de Cola ,  Sebastian Dziadek ,  Jesus Miguel Fernandez Hernandez ,  Hans-Peter Josel ,  Christoph Seidel

IPC: C07F15/00 ,  C09K11/07 ,  G01N33/58

CPC classification number: C07F15/0033 ,  C09K11/06 ,  C09K11/07 ,  C09K2211/1003 ,  C09K2211/1007 ,  C09K2211/1011 ,  C09K2211/1029 ,  C09K2211/1059 ,  C09K2211/1074 ,  C09K2211/14 ,  C09K2211/185 ,  G01N33/582

Abstract: The present invention relates to novel iridium-based Ir(III) luminescent complexes, conjugates comprising these complexes as a label and their application, e.g. in the electrochemiluminescence based detection of an analyte.

公开(公告)号：US08962338B2

公开(公告)日：2015-02-24

申请号：US13630665

申请日：2012-09-28

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Hans-Peter Josel ,  Hans-Joachim Kytzia ,  Matthias Muecke

IPC: G01N21/75 ,  G01N33/84 ,  C07C229/18

CPC classification number: G01N33/84 ,  C07C229/18

Abstract: Reagents and methods for determination of calcium within a sample. Specific embodiments include a reagent for determination of calcium comprising a mono-nitro substituted BAPTA-type chelator (BAPTA=1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid). Methods for accurate determination of calcium in a sample, such as a blood, whole blood, plasma or serum or any other aqueous liquid sample including cerebrospinal fluid, lymph, salivary juice or urine. Embodiments are also useful for clinical diagnoses.

Abstract translation: 用于测定样品中钙的试剂和方法。 具体实施方案包括用于测定钙的试剂，其包含单硝基取代的BAPTA型螯合剂（BAPTA = 1,2-双（2-氨基苯氧基）乙烷-N，N，N'，N'-四乙酸）。 用于精确测定样品（例如血液，全血，血浆或血清）或任何其它含水液体样品（包括脑脊液，淋巴，唾液汁或尿液）的钙的方法。 实施方式也可用于临床诊断。

公开(公告)号：US11965886B2

公开(公告)日：2024-04-23

申请号：US17115069

申请日：2020-12-08

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Hans-Peter Josel ,  Dieter Heindl ,  Thomas Froehlich ,  Stefanie Froehner

IPC: G01N33/50 ,  C12N5/00 ,  C12N11/06 ,  C12N11/089 ,  C12N11/14 ,  G01N33/543 ,  G01N33/554 ,  G01N33/569

CPC classification number: G01N33/56972 ,  C12N5/0068 ,  C12N11/06 ,  C12N11/089 ,  C12N11/14 ,  G01N33/5005 ,  G01N33/54353 ,  G01N33/56966 ,  C12N2503/00 ,  C12N2533/20 ,  C12N2533/30

Abstract: The present invention relates to a method of immobilizing a cell on a support, the method comprising a) providing a compound or salt thereof comprising, preferably consisting of, one or more hydrophobic domains attached to a hydrophilic domain, wherein the one or more hydrophobic domains are covalently bound to said hydrophilic domain, and wherein the one or more hydrophobic domains each comprise a linear lipid, a steroid or a hydrophobic vitamin, and wherein the hydrophilic domain comprises a polyethylene glycol (PEG) moiety, and wherein the compound comprises a linking group; b) contacting a cell with the compound under conditions allowing the interaction of the compound with the membrane of the cell, thereby immobilizing the linking group on the surface of the cell; and c) contacting the linking group immobilized on the cell with a support capable of binding the linking group, thereby immobilizing the cell on the support.

公开(公告)号：US20230160904A1

公开(公告)日：2023-05-25

申请号：US17991366

申请日：2022-11-21

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Robert Hahn ,  Dieter Heindl ,  Hans-Peter Josel ,  Uwe Kobold ,  Hannes Kuchelmeister ,  Simon Loibl ,  Heribert Maerz ,  Giuseppe Prencipe ,  Martin Rempt

IPC: G01N33/68 ,  C07C243/34 ,  C07D401/04 ,  C07D213/55 ,  C07D213/74 ,  C07D213/56

CPC classification number: G01N33/6848 ,  C07C243/34 ,  C07D401/04 ,  C07D213/55 ,  C07D213/74 ,  C07D213/56 ,  G01N2560/00

Abstract: The present invention relates to compounds which are suitable to be used in mass spectrometry as well as methods of mass spectrometric determination of analyte molecules using said compounds.

公开(公告)号：US11267827B2

公开(公告)日：2022-03-08

申请号：US16284346

申请日：2019-02-25

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Hans-Peter Josel ,  Tobias Oelschlaegel ,  Giuseppe Prencipe ,  Luisa De Cola ,  Elena Longhi ,  Chien-Wei Hsu

IPC: C07F7/08 ,  C09K11/06 ,  B82Y15/00 ,  B82Y30/00 ,  B82Y40/00

Abstract: The present disclosure relates to novel multifunctionalized silicon nanoparticles, to processes for their preparation and to compositions comprising the novel multifunctionalized silicon nanoparticles. The disclosure also relates to the use of the novel multifunctionalized silicon nanoparticles in electrochemiluminescence based detection methods and in the in vitro detection of an analyte. In particular, the disclosure relates to methods for measuring an analyte by in vitro methods employing the novel multifunctionalized silicon nanoparticles.

公开(公告)号：US20210198217A1

公开(公告)日：2021-07-01

申请号：US17155020

申请日：2021-01-21

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Dieter Heindl ,  Hans-Peter Josel ,  Uwe Kobold ,  Christoph Seidel ,  Martin Rempt ,  Andreas Leinenbach ,  Giuseppe Prencipe ,  Silvia Baecher ,  Simon Ferdinand Loibl ,  Anna-Skrollan Geiermann ,  Jelena Milic ,  Nicole Pirkl

IPC: C07D249/06 ,  C07D257/04 ,  C07D203/04 ,  G01N30/72

Abstract: The present invention relates to reagents which are suitable to be used in mass spectrometry as well as methods of mass spectrometric determination of analyte molecules using said reagents.

公开(公告)号：US20190309091A1

公开(公告)日：2019-10-10

申请号：US16450186

申请日：2019-06-24

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Michael Gerg ,  Lars Hillringhaus ,  Klaus Hirzel ,  Caroline Dorothea Hojer ,  Hans-Peter Josel ,  Christoph Seidel ,  Michael Schraeml ,  Leopold Von Proff

IPC: C07K16/44 ,  G01N33/543

Abstract: The present invention relates to a monoclonal antibody capable of binding to biotin. In one embodiment the monoclonal antibody according to the invention also does not bind to a biotin moiety on a biotinylated molecule, wherein the biotin moiety is attached to the molecule via the carbon atom of the carboxyl function of the valeric acid moiety of biotin. Also disclosed is a method for generation of an antibody as disclosed herein. The monoclonal antibody according to the invention is of specific use in a method for measuring an analyte in a sample, wherein a (strept)avidin/biotin pair is used to bind a biotinylated analyte specific binding agent to a (strept)avidin coated solid phase.