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    TRANSGENIC SYSTEMS FOR THE MANUFACTURE OF POLY(2-HYDROXY-BUTYRATE-CO-3-HYDROXYHEXANOATE)
    11.
    发明申请
    TRANSGENIC SYSTEMS FOR THE MANUFACTURE OF POLY(2-HYDROXY-BUTYRATE-CO-3-HYDROXYHEXANOATE) 失效
    用于制备聚（2-羟基丁酸酯-3-羟基己酸酯）的转基因系统

    公开(公告)号：US20100311131A1

    公开(公告)日：2010-12-09

    申请号：US12364293

    申请日：2009-02-02

    申请人： Lara Madison ,  Gjalt W. Huisman ,  Oliver P. Peoples

    发明人： Lara Madison ,  Gjalt W. Huisman ,  Oliver P. Peoples

    IPC分类号： C12P7/62

    CPC分类号： C12N9/88 ,  C12N9/0008 ,  C12P7/625

    摘要： Methods for engineering transgenic organisms that synthesize polyhydroxyalkanoates (PHAs) containing 3-hydroxyhexanoate as comonomer have been developed. These processes are based on genetically engineered bacteria such as Escherichia coli or in plant crops as production systems which include PHA biosynthetic genes from PHA producers. In a preferred embodiment of the method, additional genes are introduced in wild type or transgenic polyhydroxybutyrate (PHB) producers, thereby creating new strains that synthesize 3HH monomers which are incorporated into PHAs. The 3HH monomer preferably is derived in microbial systems using butanol or butyrate as feedstocks, which are precursors of 3-hydroxyhexanoyl-CoA. Pathways for in vivo production of butyrol-CoA specifically encompassing butyryl-CoA dehydrogenase activity are provided.

    摘要翻译： 已经开发了合成含有3-羟基己酸作为共聚单体的聚羟基链烷酸酯（PHA）的转基因生物的工程方法。 这些方法基于遗传工程细菌如大肠杆菌或植物作物，作为包括PHA生物合成基因的生产系统。 在该方法的优选实施方案中，在野生型或转基因聚羟基丁酸酯（PHB）生产者中引入另外的基因，从而产生合成并入PHA的3HH单体的新菌株。 3HH单体优选在使用丁醇或丁酸盐作为原料的微生物系统中衍生，其是3-羟基己酰基-CoA的前体。 提供了具体包含丁酰辅酶A脱氢酶活性的丁酰-coA的体内生产途径。

    Transgenic systems for the manufacture of poly (3-hydroxy-butyrate-co-3-hydroxyhexanoate)
    13.
    发明授权
    Transgenic systems for the manufacture of poly (3-hydroxy-butyrate-co-3-hydroxyhexanoate) 失效
    用于制备聚（3-羟基 - 丁酸酯 - 共-3-羟基己酸酯）的转基因系统

    公开(公告)号：US07455999B2

    公开(公告)日：2008-11-25

    申请号：US09235875

    申请日：1999-01-22

    申请人： Lara Madison ,  Gjalt W. Huisman ,  Oliver P. Peoples

    发明人： Lara Madison ,  Gjalt W. Huisman ,  Oliver P. Peoples

    IPC分类号： C12N1/21 ,  C12N1/38 ,  C12N15/09 ,  C12N15/31 ,  C12P1/04

    CPC分类号： C12N9/88 ,  C12N9/0008 ,  C12P7/625

    摘要： Methods for engineering transgenic organisms that synthesize polyhydroxyalkanoates (PHAs) containing 3-hydroxyhexanoate as comonomer have been developed. These processes are based on genetically engineered bacteria such as Escherichia coli or in plant crops as production systems which include PHA biosynthetic genes from PHA producers. In a preferred embodiment of the method, additional genes are introduced in wild type or transgenic polyhydroxybutyrate (PHB) producers, thereby creating new strains that synthesize 3HH monomers which are incorporated into PHAs. The 3HH monomer preferably is derived in microbial systems using butanol or butyrate as feedstocks, which are precursors of 3-hydroxyhexanoyl-CoA. Pathways for in vivo production of butyrol-CoA specifically encompassing butyryl-CoA dehydrogenase activity are provided.

    摘要翻译： 已经开发了合成含有3-羟基己酸作为共聚单体的聚羟基链烷酸酯（PHA）的转基因生物的工程方法。 这些方法基于遗传工程细菌如大肠杆菌或植物作物，作为包括PHA生物合成基因的生产系统。 在该方法的优选实施方案中，在野生型或转基因聚羟基丁酸酯（PHB）生产者中引入另外的基因，从而产生合成并入PHA的3HH单体的新菌株。 3HH单体优选在使用丁醇或丁酸盐作为原料的微生物系统中衍生，其是3-羟基己酰基-CoA的前体。 提供了具体包含丁酰辅酶A脱氢酶活性的丁酰-coA的体内生产途径。

    Biological systems for manufacture of polyhydroxyalkanoate polymers containing 4-hydroxyacids
    15.
    发明授权
    Biological systems for manufacture of polyhydroxyalkanoate polymers containing 4-hydroxyacids 失效
    用于制造含有4-羟基酸的聚羟基链烷酸酯聚合物的生物体系

    公开(公告)号：US07081357B2

    公开(公告)日：2006-07-25

    申请号：US10773916

    申请日：2004-02-06

    申请人： Gjalt W. Huisman ,  Frank Skraly ,  David P. Martin ,  Oliver P. Peoples

    发明人： Gjalt W. Huisman ,  Frank Skraly ,  David P. Martin ,  Oliver P. Peoples

    IPC分类号： C12P7/62 ,  C12N15/53 ,  C12N15/54

    CPC分类号： C12N9/0008 ,  C08G63/06 ,  C12N9/1029 ,  C12N9/1096 ,  C12N15/8243 ,  C12P7/625

    摘要： The gene encoding a 4-hydroxybutyryl-CoA transferase has been isolated from bacteria and integrated into the genome of bacteria also expressing a polyhydroxyalkanoate synthase, to yield an improved production process for 4HB-containing polyhydroxyalkanoates using transgenic organisms, including both bacteria and plants. The new pathways provide means for producing 4HB containing PHAs from cheap carbon sources such as sugars and fatty acids, in high yields, which are stable. Useful strains are obtaining by screening strains having integrated into their genomes a gene encoding a 4HB-CoA transferase and/or PHA synthase, for polymer production. Processes for polymer production use recombinant systems that can utilize cheap substrates. Systems are provided which can utilize amino acid degradation pathways, α-ketoglutarate, or succinate as substrate.

    摘要翻译： 已经从细菌中分离出编码4-羟基丁酰辅酶A转移酶的基因，并整合到也表达聚羟基链烷酸酯合成酶的细菌的基因组中，以产生使用包括细菌和植物在内的转基因生物体的含4HB的聚羟基链烷酸酯的改进的生产方法。 新途径提供了从低廉的碳源（如糖和脂肪酸）生产含有PHB的4HB的方法，其产率高，稳定。 有用的菌株是通过筛选在其基因组中整合了编码4HB-CoA转移酶和/或PHA合成酶的基因的聚合物生产获得的菌株。 用于聚合物生产的方法使用可以利用廉价底物的重组体系。 提供可以利用氨基酸降解途径的系统，α-酮戊二酸或琥珀酸作为底物。