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    Apparatus and method for differentiating multiple fluorescence signals by excitation wavelength
    11.
    发明授权
    Apparatus and method for differentiating multiple fluorescence signals by excitation wavelength 有权
    用于通过激发波长区分多个荧光信号的装置和方法

    公开(公告)号：US08809040B2

    公开(公告)日：2014-08-19

    申请号：US13333788

    申请日：2011-12-21

    申请人： Howard G. King ,  Steven J. Boege ,  Eugene F. Young ,  Mark F. Oldham

    发明人： Howard G. King ,  Steven J. Boege ,  Eugene F. Young ,  Mark F. Oldham

    IPC分类号： C12M1/34 ,  C12M3/00 ,  G01N21/64

    CPC分类号： G01N21/6428 ,  B01L7/52 ,  B01L2300/1805 ,  G01N21/6452 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6441 ,  G01N2201/062 ,  G01N2201/0627 ,  G01N2201/0628 ,  Y10S435/808

    摘要： An apparatus and method are provided for differentiating multiple detectable signals by excitation wavelength. The apparatus can include a light source that can emit respective excitation light wavelengths or wavelength ranges towards a sample in a sample retaining region, for example, in a well. The sample can contain two or more detectable markers, for example, fluorescent dyes, each of which can be capable of generating increased detectable emissions when excited in the presence of a target component. The detectable markers can have excitation wavelength ranges and/or emission wavelength ranges that overlap with the ranges of the other detectable markers. A detector can be arranged for detecting an emission wavelength or wavelength range emitted from a first marker within the overlapping wavelength range of at least one of the other markers.

    摘要翻译： 提供一种用于通过激发波长区分多个可检测信号的装置和方法。 该装置可以包括可以例如在一个井中的样品保留区域中的样品发射相应的激发光波长或波长范围的光源。 样品可以包含两种或更多种可检测的标记，例如荧光染料，其中当荧光染料在靶组分存在下被激发时，其能够产生增加的可检测的发射。 可检测标记物可以具有与其它可检测标记物的范围重叠的激发波长范围和/或发射波长范围。 可以设置检测器，用于检测在至少一个其他标记的重叠波长范围内从第一标记发射的发射波长或波长范围。

    Scanning system and method for imaging and sequencing
    16.
    发明授权
    Scanning system and method for imaging and sequencing 有权
    扫描系统和成像和测序方法

    公开(公告)号：US08834797B2

    公开(公告)日：2014-09-16

    申请号：US12384516

    申请日：2009-04-06

    申请人： Mark F. Oldham ,  Eric S. Nordman ,  Hongye Sun ,  Steven J. Boege

    发明人： Mark F. Oldham ,  Eric S. Nordman ,  Hongye Sun ,  Steven J. Boege

    IPC分类号： C12Q1/68 ,  G01N33/52

    CPC分类号： G01N21/6486 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6456

    摘要： A scanning detection system is provided in which emissions from locations in a flow cell are detected. In some embodiments, the system can include an excitation source, a photocleavage source, and modulating optics configured to cause an excitation beam generated by the excitation source to irradiate a first group of the fixed locations and to cause a photocleavage beam generated by the photocleavage source to irradiate a second group of the fixed locations, which is separate and apart from the first group of fixed locations. Methods of detecting sequencing reactions using such a system are also provided.

    摘要翻译： 提供了一种扫描检测系统，其中检测到流动池中的位置的发射。 在一些实施例中，系统可以包括激发源，光切割源和调制光学器件，其被配置为使得由激发源产生的激发光束照射第一组固定位置并引起由光切割源产生的光切割束 以照射与第一组固定位置分开并分开的第二组固定位置。 还提供了使用这种系统检测测序反应的方法。