公开(公告)号：US20050266450A1

公开(公告)日：2005-12-01

申请号：US11106612

申请日：2005-04-15

申请人： Yoshihiko Makino ,  Toshihiro Mori ,  Yoshihide Iwaki

发明人： Yoshihiko Makino ,  Toshihiro Mori ,  Yoshihide Iwaki

IPC分类号： C12P19/34 ,  C12Q1/42 ,  C12Q1/68 ,  G01N33/52

CPC分类号： G01N33/523 ,  C12Q1/42 ,  C12Q1/6816 ,  C12Q1/6869 ,  C12Q2565/625 ,  C12Q2565/301 ,  C12Q2521/543 ,  C12Q2533/101

摘要： An object of the present invention is to provide a method for analyzing a target nucleic acid fragment which can be simply and swiftly carried out by using a small apparatus, a kit for analyzing a target nucleic acid fragment using the method for analysis, and a dry analytical element for quantifying pyrophosphoric acid. The present invention provides a method for analyzing pyrophosphoric acid generated upon polymerase elongation reaction based on certain nucleotide sequence of a target nucleic acid, a kit for analysis for carrying out the above mentioned method for analysis, and a dry analytical element for quantifying pyrophosphoric acid.

摘要翻译： 本发明的目的是提供一种用于分析目标核酸片段的方法，其可以通过使用小型装置简单快速地进行，使用该分析方法分析靶核酸片段的试剂盒和干燥的 用于定量焦磷酸的分析元素。 本发明提供一种基于靶核酸的某些核苷酸序列分析聚合酶延伸反应产生的焦磷酸的方法，用于进行上述分析方法的分析用试剂盒和定量焦磷酸的干燥分析元素。

公开(公告)号：US08511482B2

公开(公告)日：2013-08-20

申请号：US11630135

申请日：2005-09-15

申请人： Hideaki Tanaka ,  Yoshihiko Makino

发明人： Hideaki Tanaka ,  Yoshihiko Makino

IPC分类号： B01D71/16 ,  B01D39/16 ,  B01D39/14 ,  B01D71/12 ,  C02F1/44

CPC分类号： B01D67/0013 ,  B01D15/00 ,  B01D15/08 ,  B01D67/0016 ,  B01D67/0093 ,  B01D71/12 ,  B01D2323/08 ,  B01D2323/22 ,  B01D2325/02 ,  B01D2325/022 ,  B01D2325/12 ,  B01J20/26 ,  B01J20/265 ,  B01J20/28033 ,  C08J9/28

摘要： A method of producing a porous membrane, the method comprising: casting a polymer solution, in which a polymer is dissolved in a mixture of a good solvent, a poor solvent and a non-solvent, over a support, so as to form a casted polymer solution; drying the casted polymer solution, so as to form a cast film; and subjecting the cast film to a phase separation, wherein the porous membrane is produced under a condition where a temperature of a casted surface is lower than a temperature of the polymer solution, and each of a temperature change of the polymer solution and a temperature change of the casted surface is kept within ±3.0° C.

摘要翻译： 一种多孔膜的制造方法，其特征在于，在载体上将聚合物溶解在良溶剂，不良溶剂和非溶剂的混合物中的聚合物溶液中，形成铸塑 聚合物溶液; 干燥铸塑聚合物溶液，以形成流延膜; 并对流延膜进行相分离，其中在浇铸表面的温度低于聚合物溶液的温度的条件下制备多孔膜，并且聚合物溶液的温度变化和温度变化 的铸造表面保持在±3.0℃

公开(公告)号：US07572578B2

公开(公告)日：2009-08-11

申请号：US10568101

申请日：2004-09-08

申请人： Toshihiro Mori ,  Yoshihiko Makino ,  Rie Hando ,  Yumiko Takeshita ,  Hiroko Inomata

发明人： Toshihiro Mori ,  Yoshihiko Makino ,  Rie Hando ,  Yumiko Takeshita ,  Hiroko Inomata

IPC分类号： C12Q1/68

CPC分类号： C12N15/1006 ,  C12N15/1017

摘要： The present invention is a method for isolating and purifying a nucleic acid, where generation of foams is able to be suppressed whereby the isolation and purification of a nucleic acid are easily and efficiently carried out, the method for isolating and purifying a nucleic acid comprising the step of: (1) contacting a sample solution containing nucleic acid to a solid phase to adsorb the nucleic acid onto the solid phase; (2) contacting a washing solution to the solid phase to wash the solid phase in such a state that the nucleic acid is adsorbed; and (3) contacting an elution solution to the solid phase to desorb the nucleic acid, wherein the sample solution containing nucleic acid contains an antifoaming agent.

摘要翻译： 本发明是分离和纯化核酸的方法，其中能够抑制泡沫的产生，从而容易且有效地进行核酸的分离和纯化，分离和纯化包含 步骤：（1）将含有核酸的样品溶液与固相接触以将核酸吸附到固相上; （2）将洗涤溶液与固相接触以在核酸被吸附的状态下洗涤固相; 和（3）使洗脱溶液与固相接触以解吸核酸，其中含有核酸的样品溶液含有消泡剂。

公开(公告)号：US20050277152A1

公开(公告)日：2005-12-15

申请号：US11155469

申请日：2005-06-20

申请人： Yoshihiko Makino ,  Toshihiro Mori

发明人： Yoshihiko Makino ,  Toshihiro Mori

IPC分类号： G01N30/00 ,  C07H21/00 ,  C12M1/00 ,  C12N15/09 ,  C12N15/10 ,  C12Q1/68 ,  C12M1/34

CPC分类号： C12N15/1006 ,  C12Q1/6806 ,  C12Q2565/137

摘要： An object of the present invention is to provide a method for separation and purification of nucleic acid, which has excellent separating properties, high washing efficiency, and easy processability, which uses mass-producible solid phases with substantially uniform separating capacities, and wherein procedures can be automated, and a unit for separation and purification of nucleic acid suitable for implementing this method. The object of the present invention was attained by a method for separation and purification of nucleic acid comprising the steps of adsorbing nucleic acid onto a solid phase and desorbing the nucleic acid from the solid phase, wherein the solid phase comprises a base material in which a graft polymer chain having a hydrophilic group is bonded onto the surface; and a unit for separation and purification of nucleic acid which comprises, in a container having at least two openings, a solid phase of a base material in which a graft polymer chain having a hydrophilic group is bonded onto its surface.

摘要翻译： 本发明的目的是提供一种分离和纯化核酸的方法，其具有优异的分离性能，高洗涤效率和易加工性，其使用具有基本上均匀分离能力的大量生产的固相，并且其中程序可以 自动化，以及用于分离和纯化适用于实施该方法的核酸的单元。 本发明的目的是通过核酸的分离和纯化方法来实现的，其包括将核酸吸附到固相上并从固相中解吸核酸的步骤，其中固相包括基质材料，其中 具有亲水基团的接枝聚合物链被结合到表面上; 以及用于分离和纯化核酸的单元，其在具有至少两个开口的容器中包含其中具有亲水基团的接枝聚合物链键合到其表面上的基材的固相。

公开(公告)号：US5393493A

公开(公告)日：1995-02-28

申请号：US993459

申请日：1992-12-15

申请人： Yoshihiko Makino ,  Kaoru Terashima ,  Toru Kitani ,  Naofumi Hora

发明人： Yoshihiko Makino ,  Kaoru Terashima ,  Toru Kitani ,  Naofumi Hora

IPC分类号： G01N31/22 ,  B01L3/00 ,  B01L99/00 ,  C12Q1/48 ,  C12Q1/60 ,  G01N21/78 ,  G01N33/49 ,  G01N33/52 ,  G01N33/92

CPC分类号： G01N33/525 ,  C12Q1/48 ,  C12Q1/60

摘要： An integral multilayer analytical element in which a first fibrous porous layer, a nonfibrous porous layer, and a second fibrous porous layer superposed in this order on a water-impermeable light-transmissive support the first fibrous porous-layer having a larger pore size than the nonfibrous porous layer. The above three porous layers are integrally laminated to each other substantially closely by an adhesive discontinuously disposed so as to form microspaces continuing through from one layer to the next so as not to interfere with the approximately uniform permeation of liquid. A reagent composition which produces an optically detectable change in the presence of an analyte is incorporated in at least one of said three porous layers. When a nonporous reagent layer is incorporated on the support in the above analytical element, the reagent composition is incorporated in the nonporous reagent layer. By using the analytical element of the invention, the analytical values of various analytes of blood samples can be obtained independently of hematocrit values from whole blood samples and blood plasma samples in the range of the hematocrit values of 0% to 55%.

摘要翻译： 一种整体的多层分析元件，其中第一纤维多孔层，非纤维多孔层和第二纤维多孔层依次叠加在不透水的透光性上，支撑第一纤维多孔层，其孔径大于 非纤维多孔层。 上述三个多孔层通过不连续地布置的粘合剂基本上紧密地彼此层压，以便形成从一层到下一层的连续通过的微孔，以便不干扰液体的大致均匀渗透。 在分析物存在下产生可光学检测的变化的试剂组合物结合在所述三个多孔层中的至少一个中。 当在上述分析元件中的载体上并入无孔试剂层时，将试剂组合物并入无孔试剂层。 通过使用本发明的分析元件，可以在血细胞比容值为0％至55％的范围内，独立于来自全血样品和血浆样品的血细胞比容值而独立地获得血液样品的各种分析物的分析值。

公开(公告)号：US07682818B2

公开(公告)日：2010-03-23

申请号：US10808411

申请日：2004-03-25

申请人： Toshihiro Mori ,  Yoshihiko Makino

发明人： Toshihiro Mori ,  Yoshihiko Makino

IPC分类号： C12M1/34 ,  C12Q1/68 ,  C12P19/34 ,  G01N15/06 ,  C07H21/02

CPC分类号： C12N15/1003 ,  B01L3/0275 ,  B01L2200/0631 ,  B01L2300/0681 ,  B01L2400/0478 ,  B01L2400/0605 ,  G01N1/405

摘要： The present invention provides an apparatus for separating and purifying nucleic acids, which comprises: a cylindrical syringe having a leading end part in which a first opening part is formed, a base end part in which a second opening part is formed and an accommodation part between said first opening part and second opening part, the accommodation part being able to hold liquid therein; and a solid phase-holding member connected to said leading end part, a flow hole being formed at the leading end side of the solid phase-holding member; wherein a solid phase comprised of an organic polymer having a hydroxyl group on the surface thereof is accommodated in said solid phase-holding member, the solid phase being able to adsorb and desorb nucleic acids in a sample solution; and wherein a pressure sensor capable of detecting the pressure in the accommodation part is connected.

摘要翻译： 本发明提供了一种分离和纯化核酸的装置，包括：圆柱形注射器，其具有形成有第一开口部的前端部，形成有第二开口部的基端部和第二开口部之间的容纳部， 所述第一开口部分和第二开口部分，所述容纳部分能够将液体保持在其中; 以及固相保持构件，其连接到所述前端部，流动孔形成在所述固相保持构件的前端侧; 其中由表面具有羟基的有机聚合物构成的固相被容纳在所述固相保持部件中，所述固相能够吸附和解吸样品溶液中的核酸; 并且其中连接有能够检测所述容纳部中的压力的​​压力传感器。

公开(公告)号：US20080275228A1

公开(公告)日：2008-11-06

申请号：US11547093

申请日：2005-03-28

申请人： Toshihiro Mori ,  Yoshihiko Makino

发明人： Toshihiro Mori ,  Yoshihiko Makino

IPC分类号： C07H21/00 ,  C12M1/00

CPC分类号： C12N15/1006 ,  B01D15/14 ,  B01D15/163 ,  B01J20/26 ,  B01J20/262 ,  B01J20/265 ,  B01J20/28014 ,  B01J20/28033 ,  B01J20/28054 ,  C12N15/1017 ,  G01N30/32

摘要： A method of automatically isolating and purifying nucleic acid from a nucleic acid-containing specimen is provided, the method comprising: injecting a liquid into a cartridge for isolation and purification of a nucleic acid including at least two openings from one opening of the at least two openings, in which the cartridge includes a container having the at least two openings and containing a nucleic acid-adsorbent solid phase; passing the liquid through the nucleic acid-adsorbent solid phase by a pressure difference generated by a pressure generation means for generating a pressure difference between the inside and outside of the container; and discharging the liquid from the other opening of the container to the outside of the container by a pressure difference generated by the pressure generation means, wherein a pressure generated in the inside of the container by the pressure generation means is measured, a pressure change velocity and a pressure change acceleration are calculated on the basis of the value of the measured pressure, and the timing of completion of discharge of the liquid from the container is determined by use of a temporal change pattern of at least one of the measured pressure, the pressure change velocity and the pressure change acceleration.

摘要翻译： 提供了一种从含核酸样品自动分离和纯化核酸的方法，所述方法包括：将液体注入用于分离和纯化核酸的液体，所述核酸包括至少两个开口的至少两个开口 开口，其中所述盒包括具有所述至少两个开口并且包含核酸 - 吸附剂固相的容器; 通过由压力产生装置产生的压力差使液体通过核酸 - 吸附剂固相，用于产生容器内部和外部之间的压力差; 并且通过由压力产生装置产生的压力差将液体从容器的另一个开口排出到容器的外部，其中测量由压力产生装置在容器内部产生的压力，压力变化速度 并且基于测量的压力的值来计算压力变化加速度，并且通过使用测量压力中的至少一个的时间变化模式来确定来自容器的液体的排出的完成时间， 压力变化速度和压力变化加速度。

公开(公告)号：US07252939B2

公开(公告)日：2007-08-07

申请号：US10621412

申请日：2003-07-18

申请人： Toshihiro Mori ,  Yoshihiko Makino

发明人： Toshihiro Mori ,  Yoshihiko Makino

IPC分类号： C12Q1/68 ,  G01N33/53 ,  G01N33/567 ,  C07H21/02

CPC分类号： C12N15/1006 ,  C12N15/1017

摘要： An object of the present invention is to provide a method for separating and purifying a nucleic acid by adsorbing the nucleic acid in a test sample to a surface of a solid phase and desorbing the nucleic acid by washing and the like. The present invention provides a method for separating and purifying RNA from a nucleic acid mixture, comprising a step of: adsorbing and desorbing a nucleic acid in the nucleic acid mixture containing RNA and DNA to and from a solid phase of an organic macromolecule.

摘要翻译： 本发明的目的是提供一种通过将测试样品中的核酸吸附到固相表面并通过洗涤等解吸核酸来分离和纯化核酸的方法。 本发明提供了从核酸混合物中分离和纯化RNA的方法，包括以下步骤：在含有有机高分子的固相的RNA和DNA的核酸混合物中吸附和解吸核酸。

公开(公告)号：US20050019790A1

公开(公告)日：2005-01-27

申请号：US10777585

申请日：2004-02-12

申请人： Yoshihide Iwaki ,  Yoshihiko Makino ,  Hiroshi Shinoki ,  Satoru Kuhara ,  Kosuke Tashiro ,  Shigeru Muta

发明人： Yoshihide Iwaki ,  Yoshihiko Makino ,  Hiroshi Shinoki ,  Satoru Kuhara ,  Kosuke Tashiro ,  Shigeru Muta

IPC分类号： G01N33/53 ,  B01J19/00 ,  C12M1/00 ,  C12N15/09 ,  C40B40/06 ,  C40B60/14 ,  G01N33/566 ,  G01N37/00 ,  C12Q1/68 ,  B05D3/00 ,  C12M1/34

CPC分类号： B01J19/0046 ,  B01J2219/00351 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00689 ,  B01J2219/00722 ,  C40B40/06 ,  C40B60/14

摘要： A micro-array for analysis of DNA is prepared by the steps of spotting onto a solid carrier in its predetermined area in which plural reactive groups are fixed an aqueous solution which contains a thickening agent and probe molecules (e.g., DNA fragments) having a group reactive with the reactive group of the solid carrier to produce covalent bonding; spotting onto the solid carrier in a different area having the same reactive groups an aqueous solution (same or different); incubating the aqueous solution-spotted solid carrier to produce the covalent bondings; and washing the solid carrier with an aqueous medium to remove the thickening agent from the solid carrier. An electrostatic bonding can be utilized in place of the covalent bonding.

摘要翻译： 用于分析DNA的微阵列通过以下步骤制备：将固定载体固定在其中固定有多个反应性基团的预定区域的固体载体上，所述固定载体含有增稠剂的水溶液和具有基团的探针分子（例如，DNA片段） 与固体载体的反应性基团反应产生共价键; 在具有相同反应性基团的不同区域的固体载体上发现水溶液（相同或不同）; 孵育水溶液点固体载体以产生共价键; 并用水介质洗涤固体载体以从固体载体中除去增稠剂。 可以使用静电键来代替共价键。

公开(公告)号：US5130258A

公开(公告)日：1992-07-14

申请号：US643831

申请日：1991-01-18

申请人： Yoshihiko Makino ,  Masashi Ogawa

发明人： Yoshihiko Makino ,  Masashi Ogawa

IPC分类号： G01N21/27 ,  G01N21/78 ,  G01N33/487 ,  G01N33/49 ,  G01N33/52

CPC分类号： G01N33/525 ,  G01N21/272 ,  G01N33/491 ,  G01N21/78 ,  Y10S435/805 ,  Y10S435/81 ,  Y10S436/805 ,  Y10S436/808 ,  Y10S436/824 ,  Y10T436/255

摘要： A method of quantitatively analyzing an analyte contained in a whole blood sample, wherein a dry multi-layered analysis element is used. The method provides particular merits when the used multi-layered analysis element has no light-shielding layer which is interposed, in the conventional analysis element, between a coloring reagent layer and a blood cell separating layer, so that red coloring matters of blood cells are detected from the support side during the step of measuring the optical density of the reflected light. After the optical density due to coloring matters of blood cells trapped by the blood cell separating layer has reached a constant level or background density, the changing rate in optical density is measured and then the measured changing rate is converted to the corresponding content or activity of the analyte through a colorimetric operation, or the total change in optical density is measured and then the substantially constant background density is subtracted therefrom to know the change in optical density caused by a coloring dye or like material formed in the coloring reagent layer in the presence of the analyte, followed by a similar conversion operation performed on the basis of the principle of colorimetry, to determine the content or activity of the analyte.

摘要翻译： 一种定量分析全血样品中包含的分析物的方法，其中使用干燥的多层分析元件。 当使用的多层分析元件在传统分析元件中没有遮光层在着色剂层和血细胞分离层之间时，该方法提供了特别的优点，使得血细胞的红色着色物质为 在测量反射光的光密度的步骤期间从支撑侧检测到。 在由血细胞分离层捕获的血细胞的着色物质的光密度达到一定水平或背景浓度后，测定光密度的变化率，然后将测定的变化率转换成相应的含量或活性 通过比色操作的分析物或光密度的总变化被测量，然后从其中减去基本上恒定的背景密度，以了解由存在着色剂层中形成的着色染料或类似材料引起的光密度的变化 的分析物，然后根据比色法原理进行类似的转化操作，以确定分析物的含量或活性。