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    Focused acoustics for detection and sorting of fluid volumes
    12.
    发明授权
    Focused acoustics for detection and sorting of fluid volumes 失效
    用于检测和分选流体体积的聚焦声学

    公开(公告)号：US06849423B2

    公开(公告)日：2005-02-01

    申请号：US10040926

    申请日：2001-12-28

    申请人： Mitchell W. Mutz ,  Richard N. Ellson ,  David Soong-Hua Lee

    发明人： Mitchell W. Mutz ,  Richard N. Ellson ,  David Soong-Hua Lee

    IPC分类号： B41J2/14 ,  C12M1/26 ,  C12M3/00 ,  C12N5/02 ,  G01N15/10 ,  G01N15/14 ,  G01N30/02 ,  C12Q1/02

    CPC分类号： G01N35/1074 ,  B41J2/14008 ,  C12M47/04 ,  G01N15/1056 ,  G01N29/0681 ,  G01N30/02 ,  G01N2015/1081 ,  G01N2015/1415 ,  G01N2015/142 ,  G01N2035/1039 ,  B01D2015/389

    摘要： A method is provided for acoustically ejecting from a channel or other container a plurality of fluid droplets, each of which contains one or more particles or other localized volumes. The localized volumes, which can be living cells, are ejected towards sites on a substrate surface, a container, or a channel. An integrated cell sorting and arraying system is also provided that is capable of sorting based upon cellular properties by the selective ejection of cells from a carrier fluid. The cells can be ejected with adjustable velocity and trajectory. The ejected cells can be directed to form an array, wherein each site of the array can contain a single cell. Additionally provided is a method of forming arrays of single live cells more efficiently, rapidly, flexibly, and economically than by other cell array approaches. This method permits efficient, continuous, and simultaneous sorting of cells based upon the quantitative or semiquantitative measurement of cellular properties, and also permits non-binary or severally-branched decision-making. An integrated system, which includes a processor, and methods are also provided for the detection, selection, and ejection of selected particles or circumscribed volumes, such as live cells, from a continuous stream of fluid-suspended particles or other circumscribed volumes flowing in channels.

    摘要翻译： 提供了一种用于从通道或其它容器声学喷射多个流体液滴的液滴，每个流体液滴包含一个或多个颗粒或其它局部体积。 可以是活细胞的局部体积被排出到基底表面，容器或通道上的位置。 还提供了一种综合的细胞分选和排列系统，其能够通过从载体液体中选择性地排出细胞而基于细胞特性进行分选。 可以以可调节的速度和轨迹弹出细胞。 排出的细胞可以被引导以形成阵列，其中阵列的每个位点可以包含单个细胞。 另外提供了一种形成单个活细胞阵列的方法，其比单体阵列方法更有效，快速，灵活和经济地形成。 该方法允许基于细胞性质的定量或半定量测量来对细胞进行有效，连续和同时的分选，并且还允许非二元或分支分支的决策。 还提供了一种包括处理器和方法的集成系统，用于从流体悬浮颗粒的连续流或在通道中流动的其它外接体积检测，选择和排出所选择的颗粒或外切体积（例如活细胞） 。

    Focused acoustic energy for ejecting cells from a fluid
    15.
    发明申请
    Focused acoustic energy for ejecting cells from a fluid 审中-公开
    用于从流体喷射细胞的聚焦声能

    公开(公告)号：US20020064808A1

    公开(公告)日：2002-05-30

    申请号：US09727391

    申请日：2000-11-29

    发明人： Mitchell W. Mutz ,  Richard N. Ellson

    IPC分类号： C12Q001/02 ,  C12N015/01

    CPC分类号： B41J2/14008 ,  C12M33/00 ,  G01N15/1056 ,  G01N30/02 ,  G01N35/1074 ,  G01N2015/1081 ,  G01N2015/1415 ,  G01N2015/142 ,  G01N2015/1486 ,  G01N2015/149 ,  G01N2035/1039 ,  B01D2015/389

    摘要： This invention is directed to the use of focused acoustic energy in the spatially directed ejection of cells suspended in a carrier fluid, for printing and patterning cells onto a substrate surface, for example to pattern an array of cells onto a substrate. An array of cells on a substrate surface comprising an array of substantially planar sites, with each site containing a single cell, is consequently also provided. Also disclosed are methods for the systematic generation and screening of arrays of living cells on a substrate from fluids containing one or more living cells. A method of attaching cells displaying a specific marker moiety on their surface, through specific recognition of the marker moiety by a cognate moiety that is linked to the surface is provided. Cells may be transformed to display a specific marker recognized by a corresponding cognate moiety, or the marker moiety may appear on untransformed cells. Cells displaying marker moieties may be conveniently attached to a surface functionalized with the cognate moiety. The combination of acoustic ejection and the marker moiety/cognate moiety system can be employed to select cells displaying a specific marker for adhesion to a substrate surface. The combination of several different marker moieties uniquely displayed on the cell surface of different types of cells combined with an array of different cognate moieties on a substrate may be employed in conjunction with the acoustic ejection of single living cells to create cell arrays with a specific number of cells displaying a specific marker attached to the substrate surface at a desired locale or region.

    摘要翻译： 本发明涉及聚焦声能在悬浮在载体流体中的细胞的空间定向喷射中的用途，用于将细胞印刷和图案化到衬底表面上，例如将细胞阵列图案化到衬底上。 因此，还提供了包括基本上平面的位置的阵列的衬底阵列阵列，每个位置包含单个电池。 还公开了用于从含有一种或多种活细胞的流体系统地生成和筛选底物上的活细胞阵列的方法。 提供了通过与表面连接的同源部分特异性识别标记部分来附着在其表面上显示特定标记部分的细胞的方法。 可以转化细胞以显示由对应的同源部分识别的特异性标记，或者标记部分可以出现在未转化的细胞上。 显示标记部分的细胞可以方便地连接到用同源部分官能化的表面上。 可以使用声弹出和标记部分/同源部分系统的组合来选择显示用于粘附到基底表面的特定标记物的细胞。 与不同类型细胞的细胞表面独特显示的几个不同标记部分的组合与基底上的不同同源部分的阵列结合可以与单个活细胞的声弹出结合使用以产生具有特定数目的细胞阵列 的细胞显示在期望的区域或区域附着于基底表面的特异性标记。

    Ejection of localized volumes from fluids
    16.
    发明授权
    Ejection of localized volumes from fluids 有权
    从流体喷射局部体积

    公开(公告)号：US07270986B2

    公开(公告)日：2007-09-18

    申请号：US11049206

    申请日：2005-02-01

    申请人： Mitchell W. Mutz ,  Richard N. Ellson ,  David Soong-Hua Lee

    发明人： Mitchell W. Mutz ,  Richard N. Ellson ,  David Soong-Hua Lee

    IPC分类号： C12N13/00

    CPC分类号： G01N35/1074 ,  B41J2/14008 ,  C12M47/04 ,  G01N15/1056 ,  G01N29/0681 ,  G01N30/02 ,  G01N2015/1081 ,  G01N2015/1415 ,  G01N2015/142 ,  G01N2035/1039 ,  B01D2015/389

    摘要： A method is provided for acoustically ejecting from a channel or other container a plurality of fluid droplets, each of which contains one or more particles or other localized volumes. The localized volumes, which can be living cells, are ejected towards sites on a substrate surface, a container, or a channel. An integrated cell sorting and arraying system is also provided that is capable of sorting based upon cellular properties by the selective ejection of cells from a carrier fluid. The cells can be ejected with adjustable velocity and trajectory. The ejected cells can be directed to form an array, wherein each site of the array can contain a single cell. Additionally provided is a method of forming arrays of single live cells more efficiently, rapidly, flexibly, and economically than by other cell array approaches. This method permits efficient, continuous, and simultaneous sorting of cells based upon the quantitative or semiquantitative measurement of cellular properties, and also permits non-binary or severally-branched decision-making. An integrated system, which includes a processor, and methods are also provided for the detection, selection, and ejection of selected particles or circumscribed volumes, such as live cells, from a continuous stream of fluid-suspended particles or other circumscribed volumes flowing in channels.

    摘要翻译： 提供了一种用于从通道或其它容器声学喷射多个流体液滴的液滴，每个流体液滴包含一个或多个颗粒或其它局部体积。 可以是活细胞的局部体积被排出到基底表面，容器或通道上的位置。 还提供了一种综合的细胞分选和排列系统，其能够通过从载体液体中选择性地排出细胞而基于细胞特性进行分选。 可以以可调节的速度和轨迹弹出细胞。 排出的细胞可以被引导以形成阵列，其中阵列的每个位点可以包含单个细胞。 另外提供了一种形成单个活细胞阵列的方法，其比单体阵列方法更有效，快速，灵活和经济地形成。 该方法允许基于细胞性质的定量或半定量测量来对细胞进行有效，连续和同时的分选，并且还允许非二元或分支分支的决策。 还提供了一种包括处理器和方法的集成系统，用于从流体悬浮颗粒的连续流或在通道中流动的其它外接体积检测，选择和排出所选择的颗粒或外切体积（例如活细胞） 。

    Focused acoustic ejection cell sorting system and method
    18.
    发明申请
    Focused acoustic ejection cell sorting system and method 失效
    聚焦声弹出细胞分选系统及方法

    公开(公告)号：US20020090720A1

    公开(公告)日：2002-07-11

    申请号：US10040926

    申请日：2001-12-28

    发明人： Mitchell W. Mutz ,  Richard N. Ellson ,  David Soong-Hua Lee

    IPC分类号： C12N005/02

    CPC分类号： G01N35/1074 ,  B41J2/14008 ,  C12M47/04 ,  G01N15/1056 ,  G01N29/0681 ,  G01N30/02 ,  G01N2015/1081 ,  G01N2015/1415 ,  G01N2015/142 ,  G01N2035/1039 ,  B01D2015/389

    摘要： A method is provided for acoustically ejecting from a channel or other container a plurality of fluid droplets, each of which contains one or more particles or other localized volumes. The localized volumes, which can be living cells, are ejected towards sites on a substrate surface, a container, or a channel. An integrated cell sorting and arraying system is also provided that is capable of sorting based upon cellular properties by the selective ejection of cells from a carrier fluid. The cells can be ejected with adjustable velocity and trajectory. The ejected cells can be directed to form an array, wherein each site of the array can contain a single cell. Additionally provided is a method of forming arrays of single live cells more efficiently, rapidly, flexibly, and economically than by other cell array approaches. This method permits efficient, continuous, and simultaneous sorting of cells based upon the quantitative or semiquantitative measurement of cellular properties, and also permits non-binary or severally-branched decision-making. An integrated system, which includes a processor, and methods are also provided for the detection, selection, and ejection of selected particles or circumscribed volumes, such as live cells, from a continuous stream of fluid-suspended particles or other circumscribed volumes flowing in channels.

    摘要翻译： 提供了一种用于从通道或其它容器声学喷射多个流体液滴的液滴，每个流体液滴包含一个或多个颗粒或其它局部体积。 可以是活细胞的局部体积被排出到基底表面，容器或通道上的位置。 还提供了一种综合的细胞分选和排列系统，其能够通过从载体液体中选择性地排出细胞而基于细胞特性进行分选。 可以以可调节的速度和轨迹弹出细胞。 排出的细胞可以被引导以形成阵列，其中阵列的每个位点可以包含单个细胞。 另外提供了一种形成单个活细胞阵列的方法，其比单体阵列方法更有效，快速，灵活和经济地形成。 该方法允许基于细胞性质的定量或半定量测量来对细胞进行有效，连续和同时的分选，并且还允许非二元或分支分支的决策。 还提供了一种包括处理器和方法的集成系统，用于从流体悬浮颗粒的连续流或在通道中流动的其它外接体积检测，选择和排出所选择的颗粒或外切体积（例如活细胞） 。

    Focused acoustic ejection cell sorting system and method
    19.
    发明申请
    Focused acoustic ejection cell sorting system and method 审中-公开
    聚焦声弹出细胞分选系统及方法

    公开(公告)号：US20020064809A1

    公开(公告)日：2002-05-30

    申请号：US09751666

    申请日：2000-12-28

    发明人： Mitchell W. Mutz ,  Richard N. Ellson ,  David Soong-Hua Lee

    IPC分类号： G01N001/30 ,  G01N033/48 ,  C12N015/01

    CPC分类号： B41J2/14008 ,  B01L3/0268 ,  C12M47/04 ,  G01N15/1056 ,  G01N15/1456 ,  G01N29/028 ,  G01N30/02 ,  G01N35/1074 ,  G01N2015/1081 ,  G01N2015/1415 ,  G01N2015/142 ,  G01N2015/1486 ,  G01N2015/149 ,  G01N2035/1039 ,  G01N2035/1048 ,  G01N2035/1062 ,  B01D2015/389

    摘要： A method is provided for acoustically ejecting from a container that is preferably a channel, a plurality of particles or localized volumes that can be single living cells contained in fluid droplets toward sites on a substrate surface or alternatively or in addition thereto into containers or channels for deposition at a target array site or a container or channel by acoustic ejection. An integrated cell sorting and arraying system also is provided that is capable of selective sorting, into channels or other containers substantially transected by a common plane, parallel to a surface of the fluid, transecting the container from which cells are ejected by selective ejection of cells with adjustable velocity parallel to the fluid surface, and simultaneously selectively forming an array of cells on a substrate surface comprising an array of substantially planar sites is provided, wherein each site contains a single cell. Additionally provided is a method of forming arrays of single live cells more efficiently, rapidly, flexibly and economically than by other cell array approaches, while permitting efficient, continuous and simultaneous sorting of cells based upon selection by measurement of detectible properties quantitatively or semiquantitatively, and multiple ejection target selections permitting non-binary or severally branched decision making. An integrated system and methods are also provided for ejection of selected particles or circumscribed volumes such as live cells from a continuous stream of particles or circumscribed volumes flowing in fluidic ejection channels into flowing fluidic target channels based upon selection by measurement of detectible properties quantitatively or semiquantitatively, and multiple ejection target selections permitting non-binary or severally branched decision making integrated with the measurement by way of a processor.

    摘要翻译： 提供了一种用于从容器中声学喷射的方法，所述容器优选地是通道，多个颗粒或局部体积，其可以是流体液滴中包含在流体液滴中的单个活细胞朝向基底表面上的位置，或者替代地或附加到容器或通道中， 通过声喷射在靶阵列位置沉积或容器或通道。 还提供了一种集成的细胞分选和排列系统，其能够选择性地分选到平行于流体表面的基本上由公共平面横切的通道或其它容器中，从而通过选择性排出细胞来切断细胞被排出的容器 具有平行于流体表面的可调速度，并且同时在包括基本上平面的位置的阵列的基板表面上选择性地形成单元阵列，其中每个位置包含单个单元。 另外提供了一种比通过其他细胞阵列方法更有效，快速，灵活和经济地形成单活细胞阵列的方法，同时基于通过定量或半定量测量可检测性质的选择来有效，连续和同时分选细胞，以及 多个弹出目标选择允许非二进制或多个分支的决策。 还提供了一种集成的系统和方法，用于基于通过定量或半定量测量可检测性质的选择，将选定的颗粒或诸如活细胞的外来体积从连续的颗粒流或流体喷射通道中流动的外接体积喷射到流动的流体目标通道中 以及允许通过处理器与测量集成的非二进制或多个分支决策的多个喷射目标选择。

    Acoustical filtration system
    20.
    发明授权
    Acoustical filtration system 失效
    声学过滤系统

    公开(公告)号：US3650094A

    公开(公告)日：1972-03-21

    申请号：US3650094D

    申请日：1969-12-19

    申请人： UNITED AIRCRAFT CORP

    发明人： GOODWIN RAYMOND WENDELL

    IPC分类号： B08B3/12 ,  G01N30/02 ,  B01D50/00

    CPC分类号： B08B3/12 ,  B08B2203/002 ,  G01N30/02 ,  B01D2015/389

    摘要： A fluid medium containing suspended particles is subjected to an asymmetrical sound wave having a high velocity in one direction of movement and a low velocity in the opposite direction of movement. The velocities are sufficiently great to produce square-law drag forces; and the suspended particles will be propelled through the fluid medium in a direction corresponding to the high velocity direction of the fluid medium.

    摘要翻译： 含有悬浮颗粒的流体介质经受在一个运动方向上具有高速度的不对称声波和在相反的运动方向上的低速度。 速度足够大以产生平方律拖拉力; 并且悬浮的颗粒将在对应于流体介质的高速度方向的方向上被推动通过流体介质。