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    NON-MASS DETERMINED BASE COMPOSITIONS FOR NUCLEIC ACID DETECTION
    11.
    发明申请
    NON-MASS DETERMINED BASE COMPOSITIONS FOR NUCLEIC ACID DETECTION 审中-公开
    用于核酸检测的非质子测定基质组合物

    公开(公告)号：US20150307935A1

    公开(公告)日：2015-10-29

    申请号：US14797895

    申请日：2015-07-13

    申请人： IBIS BIOSCIENCES, INC.

    发明人： Christopher C. Sappenfield

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6869 ,  C12Q2537/165 ,  C12Q2521/101 ,  C12Q2535/101 ,  C12Q2535/122 ,  C12Q2563/107 ,  C12Q2563/125 ,  C12Q2565/301 ,  C12Q2565/518 ,  C12Q2565/625 ,  C12Q2565/631

    摘要： The present invention provides systems, methods, and compositions for nucleic acid detection based on non-mass determined base compositions. For example, in certain embodiments, base count data for a template nucleic acid is generated using an approach that does not measure molecular mass of the template nucleic acid (e.g., by sequencing the template nucleic acid) and a database comprising base count entries is queried to identify the target nucleic acid. In particular embodiments, sequencing is employed which is conducted in substantially real-time.

    摘要翻译： 本发明提供了基于非质量确定的基础组合物的用于核酸检测的系统，方法和组合物。 例如，在某些实施方案中，使用不测量模板核酸分子量（例如通过测序模板核酸）的方法产生模板核酸的碱基计数数据，并且查询包含碱基计数条目的数据库 以鉴定靶核酸。 在具体实施方案中，采用基本上实时进行的测序。

    Lateral flow assays
    12.
    发明授权
    Lateral flow assays 有权
    侧流测定

    公开(公告)号：US09121849B2

    公开(公告)日：2015-09-01

    申请号：US13790229

    申请日：2013-03-08

    申请人： Rapid Pathogen Screening, Inc.

    发明人： Uma Mahesh Babu ,  Robert P. Sambursky ,  Robert W. VanDine

    IPC分类号： C12Q1/68 ,  G01N33/53

    CPC分类号： G01N33/5308 ,  C12Q1/6834 ,  Y10T436/143333 ,  C12Q2565/625 ,  C12Q2565/519 ,  C12Q2525/161

    摘要： Assays and methods including mobile tagged single stranded nucleic acid reagents pre-loaded on an analysis device, which are preferably tagged, but not labeled and are complementary to a strand (preferably the anti-sense strand in double stranded DNA targets) of the target nucleic acid. The assay also includes a running buffer that includes a dye or other detectable label that nonspecifically binds only to double stranded nucleic acids. In addition, the analysis device includes a detection zone including one or more test zones that have an immobilized tag that binds to the tag on the mobile nucleic acid reagent.

    摘要翻译： 测定和方法，包括预先加载在分析装置上的移动标记单链核酸试剂，其优选地被标记但未标记并与靶核酸的链（优选双链DNA靶的反义链）互补 酸。 该测定还包括运行缓冲液，其包括仅与双链核酸非特异性结合的染料或其它可检测标记。 此外，分析装置包括检测区域，该检测区域包括具有与移动核酸试剂上的标签结合的固定化标签的一个或多个测试区域。

    Nucleic acid detection method and nucleic acid detection kit
    14.
    发明授权
    Nucleic acid detection method and nucleic acid detection kit 有权
    核酸检测方法和核酸检测试剂盒

    公开(公告)号：US09063130B2

    公开(公告)日：2015-06-23

    申请号：US12677189

    申请日：2008-08-27

    申请人： Jun Tomono

    发明人： Jun Tomono

    IPC分类号： G01N33/53 ,  C12Q1/68 ,  G01N33/566

    CPC分类号： G01N33/5308 ,  C12Q1/6844 ,  G01N33/566 ,  Y02A50/59 ,  C12Q2565/113 ,  C12Q2563/107 ,  C12Q2565/625 ,  C12Q2565/137 ,  C12Q2565/531

    摘要： In the present invention, an amplified DNA fragment having a first substance binding site to which a first substance is specifically bindable is prepared, which amplified DNA fragment amplified by a nucleic acid amplification method. The amplified DNA fragment is concentrated by binding the amplified DNA fragment to the first substance. The concentration makes it possible to detect the DNA highly sensitively. Therefore, with the arrangement, it is possible to detect the amplified DNA fragment amplified by the nucleic acid amplification method, easily and highly accurately without requiring any special device.

    摘要翻译： 在本发明中，制备具有第一物质特异性结合的第一物质结合位点的扩增DNA片段，扩增通过核酸扩增法扩增的DNA片段。 通过将扩增的DNA片段与第一物质结合来浓缩扩增的DNA片段。 该浓度使得可以高度灵敏地检测DNA。 因此，通过该配置，可以容易且高精度地检测通过核酸扩增方法扩增的扩增的DNA片段，而无需任何特殊的装置。

    SYSTEMS AND METHODS FOR DETECTING BIOMARKERS OF INTEREST
    15.
    发明申请
    SYSTEMS AND METHODS FOR DETECTING BIOMARKERS OF INTEREST 审中-公开
    用于检测生物标志物的系统和方法

    公开(公告)号：US20140302486A1

    公开(公告)日：2014-10-09

    申请号：US14139464

    申请日：2013-12-23

    申请人： President and Fellows of Harvard College ,  University of Washington Through Its Center For Commercialization

    发明人： Georg Seelig ,  Xi Chen ,  David Yu Zhang

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6827 ,  C12Q1/682 ,  C12Q1/6823 ,  C12Q1/6876 ,  C12Q1/6886 ,  C12Q1/689 ,  C12Q2600/178 ,  C12Q2537/1373 ,  C12Q2537/155 ,  C12Q2537/157 ,  C12Q2565/625 ,  C12Q2565/101

    摘要： A detection probe for detecting single base mutations or alterations in a double stranded target nucleic acid molecule is provided. In some aspects, the detection probe may include a double-stranded probe nucleic acid molecule having a first end and a second end; at least one probe initiation toehold at the first end; at least one substance capable of emitting a detectable signal at the second end; and optionally, at least one dissociation toehold at the second end. The detection probe is designed to hybridize with a double stranded target nucleic acid in a reaction which proceeds at approximate thermodynamic equilibrium (ΔG≈0) when no single base mutations or alterations are present in the double stranded target nucleic acid molecule. The probe may be used in detection systems and methods to identifying the presence or absence of one or more single base mutations or alterations in a double-stranded nucleic acid target molecule.

    摘要翻译： 提供了用于检测双链靶核酸分子中的单碱基突变或改变的检测探针。 在一些方面，检测探针可以包括具有第一末端和第二末端的双链探针核酸分子; 在第一端至少有一个探头起始; 能够在第二端发射可检测信号的至少一种物质; 并且任选地，在第二端处至少一个离解至止。 设计检测探针与当双链靶核酸分子中没有单碱基突变或改变存在时，以近似热力学平衡（＆Dgr;G≈0）进行的反应中的双链靶核酸杂交。 探针可以用于检测系统和方法中以鉴定双链核酸靶分子中一个或多个单碱基突变或改变的存在或不存在。

    MOBILE RAPID TEST SYSTEM FOR NUCLEIC ACID ANALYSIS
    16.
    发明申请
    MOBILE RAPID TEST SYSTEM FOR NUCLEIC ACID ANALYSIS 有权
    用于核酸分析的移动快速测试系统

    公开(公告)号：US20130130257A1

    公开(公告)日：2013-05-23

    申请号：US13723315

    申请日：2012-12-21

    申请人： Timo Hillebrand ,  Claus Knippschild ,  Benjamin Jaschinsky ,  Elmara Graser

    发明人： Timo Hillebrand ,  Claus Knippschild ,  Benjamin Jaschinsky ,  Elmara Graser

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/686 ,  B01L3/5023 ,  B01L7/52 ,  B01L2200/026 ,  B01L2200/04 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/0825 ,  B01L2300/1822 ,  C12Q2565/629 ,  C12Q2565/625 ,  C12Q2565/101

    摘要： A mobile rapid test system for nucleic acid analysis. A method comprising the steps of amplification of the nucleic acids by means of rapid-PCR technology, conversion of a double-stranded amplification product into a single-stranded DNA fragment, hybridization with a labeled probe and detection of the nucleic acids on a lateral-flow test strip. A device comprising a reaction cavity which preferably consists of a thin film, inlet and outlet openings for the reaction cavity, one or more heatable sample blocks which are connected to miniaturized cooling bodies and a window for reading off the result. The lateral-flow test strip is a component of the mobile rapid test system. Operation of the instrument system requires no external power source, but only batteries or a rechargeable battery.

    摘要翻译： 一种用于核酸分析的移动快速测试系统。 一种方法，其包括以下步骤：通过快速PCR技术扩增核酸，将双链扩增产物转化成单链DNA片段，与标记的探针杂交并检测侧链上的核酸， 流动试纸条。 一种包括反应腔的装置，其优选由薄膜，用于反应空腔的入口和出口开口组成，一个或多个可连接到小型化冷却体的可加热样品块和用于读取结果的窗口。 侧流测试条是移动快速测试系统的组成部分。 仪器系统的操作不需要外部电源，只能使用电池或可充电电池。

    MASS SPECTROMETRIC METHODS FOR DETECTING MUTATIONS IN A TARGET NUCLEIC ACID
    18.
    发明申请
    MASS SPECTROMETRIC METHODS FOR DETECTING MUTATIONS IN A TARGET NUCLEIC ACID 审中-公开
    用于检测目标核酸中的突变的质谱方法

    公开(公告)号：US20110027773A1

    公开(公告)日：2011-02-03

    申请号：US12795155

    申请日：2010-06-07

    申请人： Hurbert Kõster

    发明人： Hurbert Kõster

    IPC分类号： C12Q1/68 ,  C12Q1/70

    CPC分类号： C12Q1/6872 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6837 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6862 ,  C12Q1/6883 ,  C12Q1/706 ,  G01N35/1067 ,  H01J49/00 ,  Y10T436/143333 ,  Y10T436/24 ,  C12Q2565/627 ,  C12Q2537/113 ,  C12Q2535/113 ,  C12Q2521/325 ,  C12Q2535/131 ,  C12Q2525/107 ,  C12Q2523/107 ,  C12Q2523/319 ,  C12Q2565/625 ,  C12Q2535/125

    摘要： Fast and highly accurate mass spectrometry-based processes for detecting particular nucleic acid molecules and mutations in the molecules are provided. In some embodiments, a process comprises: amplifying a nucleic acid from a biological sample; ionizing and volatilizing the amplified product; analyzing the product by mass spectrometry to determine an observed molecular mass of the product; and comparing the observed molecular mass of the product to a calculated molecular mass of at least one nucleic acid having a known sequence, wherein the calculated molecular mass of the at least one nucleic acid having a known sequence is derived from the base composition of the at least one nucleic acid having a known sequence; whereby the presence or absence of the target nucleic acid is detected based on the comparison.

    摘要翻译： 提供了用于检测特定核酸分子的快速且高度精确的基于质谱的方法，并且提供了分子中的突变。 在一些实施方案中，方法包括：从生物样品中扩增核酸; 电离和挥发扩增产物; 通过质谱分析产物以确定产物的观察分子量; 并且将所述产物的观察分子量与具有已知序列的至少一种核酸的计算分子量进行比较，其中具有已知序列的所述至少一种核酸的计算分子量衍生自所述基础组合物 至少一种具有已知序列的核酸; 由此基于比较检测目标核酸的存在或不存在。

    LATERAL FLOW NUCLEIC ACID DETECTOR
    19.
    发明申请
    LATERAL FLOW NUCLEIC ACID DETECTOR 失效
    侧流核酸检测器

    公开(公告)号：US20090305290A1

    公开(公告)日：2009-12-10

    申请号：US12502626

    申请日：2009-07-14

    申请人： Robert P. Sambursky ,  Uma Mahesh Babu ,  Robert W. VanDine

    发明人： Robert P. Sambursky ,  Uma Mahesh Babu ,  Robert W. VanDine

    IPC分类号： C12Q1/68 ,  G01N33/00 ,  G01N33/543 ,  C12M1/34

    CPC分类号： G01N33/526 ,  C12Q1/6834 ,  Y10S436/81 ,  C12Q2565/625 ,  C12Q2565/519 ,  C12Q2525/161

    摘要： Point-of-care binding assays include at least one target nucleic acid binding in a multiplex structure with at least one sequence in a partner nucleic acid associated with a label, due to complementary base pairings between at least one sequence in the target nucleic acid and at least one sequence in the partner nucleic acid. The assays overcome the inherent deficiencies of antibody-protein antigen assays. In a preferred embodiment, color tagged nucleic acid sequences are used to bind a complementary target nucleic acid. The tagged nucleic acid sequences are preferably made from deoxyribonucleotides, ribonucleotides, or peptide nucleotides.

    摘要翻译： 点护理结合测定法包括至少一种多核结构中的靶核酸与靶标核酸中的至少一个序列之间的互补碱基配对与靶核酸中的至少一个序列之间的互补碱基配对和 伴侣核酸中的至少一个序列。 该测定克服了抗体 - 蛋白质抗原测定的固有缺陷。 在优选的实施方案中，使用彩色标记的核酸序列结合互补靶核酸。 标记的核酸序列优选由脱氧核糖核苷酸，核糖核苷酸或肽核苷酸制成。