利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

193 条记录 (耗时 0.024 秒)

    Method for measuring carbon dioxide and reagent therefor
    11.
    发明授权
    Method for measuring carbon dioxide and reagent therefor 失效
    二氧化碳测定方法及其试剂

    公开(公告)号：US5547851A

    公开(公告)日：1996-08-20

    申请号：US210559

    申请日：1994-03-18

    申请人： Shizuo Hattori ,  Takahide Kishimoto ,  Yukihiro Sogabe ,  Shigenori Emi

    发明人： Shizuo Hattori ,  Takahide Kishimoto ,  Yukihiro Sogabe ,  Shigenori Emi

    IPC分类号： C12Q1/32 ,  C12Q1/527 ,  C12Q1/25

    CPC分类号： C12Q1/527 ,  Y10S435/822 ,  Y10S435/823

    摘要： A method for measuring carbon dioxide, comprising the steps of: (1) reacting bicarbonate ion in a sample with phosphoenolpyruvate carboxylase derived from an acetic acid bacterium in the presence of phosphoenolpyruvate; (2) reacting the resultant oxalacetic acid with malate dehydrogenase in the presence of NADH; and (3) measuring decreased NADH, and a reagent for measuring carbon dioxide, comprising phosphoenolpyruvate, phosphoenolpyruvate carboxylase derived from an acetic acid bacterium, malate dehydrogenase, NADH and a buffer. According to the present invention, a highly stable reagent for CO.sub.2 measurement, permitting a long-term storage in a liquid state can be provided by the use of phosphoenolpyruvate carboxylase derived from an acetic acid bacterium.

    摘要翻译： 一种测量二氧化碳的方法，包括以下步骤：（1）在磷酸烯醇丙酮酸存在下，将样品中的碳酸氢根离子与来自乙酸细菌的磷酸烯醇丙酮酸羧化酶反应; （2）在NADH存在下使所得草乙酸与苹果酸脱氢酶反应; 和（3）测量降低的NADH，和用于测量二氧化碳的试剂，其包含衍生自乙酸细菌的磷酸烯醇丙酮酸磷酸烯醇丙酮酸羧化酶，苹果酸脱氢酶，NADH和缓冲液。 根据本发明，通过使用源于乙酸细菌的磷酸烯醇丙酮酸羧化酶，可以提供高度稳定的用于CO 2测量的试剂，其允许液体中的长期储存。

    Method of supporting fractures in geologic formations and hydraulic fluid composition for same
    15.
    发明授权
    Method of supporting fractures in geologic formations and hydraulic fluid composition for same 失效
    支持地质构造中的裂缝和液压流体组成相同的方法

    公开(公告)号：US5009797A

    公开(公告)日：1991-04-23

    申请号：US450360

    申请日：1989-12-13

    申请人： Glenn S. Penny ,  Robert S. Stephens ,  Alan R. Winslow

    发明人： Glenn S. Penny ,  Robert S. Stephens ,  Alan R. Winslow

    IPC分类号： C09K8/62 ,  C09K8/90 ,  E21B43/267

    CPC分类号： C09K8/90 ,  C09K8/62 ,  E21B43/267 ,  Y10S435/823 ,  Y10S507/922

    摘要： The invention relates to hydraulic fracturing of geological formations at selected levels of wells drilled for recovery of hydrocarbons. It resides in the addition of relatively small quantities of a bacterial cellulose to hydraulic fracturing fluids to improve their rheological properties. Proppant suspension is markedly improved and friction loss through well casings is significantly reduced, resulting in lower pumping energy requirements. Computer models also indicate that formation fractures will also be propagated for greater distances as will the propped portion of the fracture. Normally only about 5-15 lb of bacterial cellulose per 1000 gallons (0.60-1.8 g/L) of fracturing fluid is needed. A preferred bacterial cellulose is one made in agitated fermenters using mutation resistant strains of a bacterium from the genus Acetobacter.

    摘要翻译： 本发明涉及在钻井回收烃的选定水平的地质地层的水力压裂。 它存在于向水力压裂液中添加相对少量的细菌纤维素以改善其流变性质。 支撑剂悬浮液显着改善，通过井套件的摩擦损失显着降低，导致较低的泵送能量需求。 计算机模型还表明，地层裂缝也将传播更长的距离，如支撑的裂缝部分。 通常，每1000加仑（0.60-1.8 g / L）的压裂液只需要约5-15 lb的细菌纤维素。 优选的细菌纤维素是使用来自醋杆菌属的细菌的突变抗性菌株的搅拌发酵罐中制备的细菌纤维素。

    Modification of cellulose normally synthesizied by cellulose-producing microorganisms
    16.
    发明授权
    Modification of cellulose normally synthesizied by cellulose-producing microorganisms 失效
    纤维素生产微生物通常合成的纤维素的改性

    公开(公告)号：US4950597A

    公开(公告)日：1990-08-21

    申请号：US198784

    申请日：1988-05-25

    申请人： Inder M. Saxena ,  Eric M. Roberts ,  R. Malcolm Brown, Jr.

    发明人： Inder M. Saxena ,  Eric M. Roberts ,  R. Malcolm Brown, Jr.

    IPC分类号： C12P19/04

    CPC分类号： C12R1/02 ,  C12P19/04 ,  Y10S435/822 ,  Y10S435/823

    摘要： The present invention involves a process for screening for and isolating spontaneously occurring or induced cellulose II-producing microorganisms. The process comprises a series of steps in various embodiments. Initially, cellulose-producing microorganisms from a first culture are plated out on a nutrient agar plate. The nutrient agar plate is then incubated to facilitate formation of colonies from single microorganisms. Samples of liquid nutrient medium are then inoculated with microorganisms from colonies having a smooth configuration, as compared to the usual rough colony configuration. The inoculated samples are then aerobically incubated to facilitate microorganism proliferation and pellicle formation. From these incubated samples are selected microorganisms, which, after a cultivation period, have proliferated but not formed a pellicle. Said selected microorganisms produce cellulose II instead of the cellulose I produced by pellicle-forming organisms.

    摘要翻译： 本发明涉及用于筛选和分离自发产生或诱导的产生纤维素II的微生物的方法。 该方法包括各种实施方案中的一系列步骤。 最初，将来自第一培养物的产生纤维素的微生物铺在营养琼脂平板上。 然后培养营养琼脂平板以促进从单一微生物形成菌落。 与通常的粗殖群配置相比，液体营养培养基的样品接种具有平滑构型的菌落的微生物。 然后将接种的样品有氧培养以促进微生物增殖和防护薄膜组织。 从这些孵育的样品中选出微生物，其在培养期后已增殖但未形成防护薄膜。 所述选择的微生物产生纤维素II，而不是由防护薄膜组织生物体产生的纤维素I。

    Sheeted products formed from reticulated microbial cellulose
    17.
    发明授权
    Sheeted products formed from reticulated microbial cellulose 失效
    由网状微生物纤维素形成的片状产品

    公开(公告)号：US4863565A

    公开(公告)日：1989-09-05

    申请号：US196127

    申请日：1988-05-19

    申请人： Donald C. Johnson ,  Amar N. Neogi

    发明人： Donald C. Johnson ,  Amar N. Neogi

    IPC分类号： A61L15/28 ,  C12P19/04

    CPC分类号： C12R1/02 ,  A61L15/28 ,  C12P19/04 ,  Y10S428/913 ,  Y10S435/823 ,  Y10T428/2922 ,  Y10T428/2965

    摘要： A method and media for producing bacterial cellulose under agitated culture conditions resulting in sustained production over an average of 70 hours of at least 0.1 g/liter per hour are achieved. A unique reticulated cellulose product is produced using the methods and conditions claimed, and may be in the form of a sheet characterized by substantial resistance to densification and great tensile strength when produced by sheet forming means.Strains of Acetobacter that are stable under agitated culture conditions and that exhibit substantially reduced gluconic and keto-gluconic acids production are described.

    摘要翻译： 在搅拌培养条件下生产细菌纤维素的方法和介质，得到平均70小时至少0.1g /升/小时的持续生产。 使用所要求保护的方法和条件制备独特的网状纤维素产品，并且可以是以片材成形方法制造时特征为显着的致密化抗拉强度的片材的形式。 描述了在搅拌培养条件下稳定并且显示出显着降低的葡萄糖酸和酮 - 葡萄糖酸生产的醋杆菌菌株。

    New restriction enzyme and process for producing the same
    18.
    发明授权
    New restriction enzyme and process for producing the same 失效
    新的限制酶及其制备方法

    公开(公告)号：US4833082A

    公开(公告)日：1989-05-23

    申请号：US905455

    申请日：1986-09-10

    申请人： Yuzo Yamada ,  Makoto Murakami

    发明人： Yuzo Yamada ,  Makoto Murakami

    IPC分类号： C12N9/16 ,  C12N9/22 ,  C12R1/02

    CPC分类号： C12N9/22 ,  Y10S435/823

    摘要： A restriction enzyme, ApaLI, which recognizes the base sequence in a double-stranded deoxyribonucleic acid molecule as shown below, and cleaves it at the arrow-marked sites, ##STR1## wherein A, G, T and C represent adenosine, guanosine, thymidine and cytidine, respectively. Also provided is a process for producing this enzyme, by growing a microorganism belonging to the genus Acetobacter.

    摘要翻译： 限制性内切酶_ALI，其识别如下所示的双链脱氧核糖核酸分子中的碱基序列，并在箭头标记位点上切割，其中A，G，T和C代表腺苷，鸟苷，胸苷 和胞苷。 还提供了通过生长属于醋杆菌属的微生物来生产该酶的方法。