公开(公告)号：US11739307B2

公开(公告)日：2023-08-29

申请号：US17055801

申请日：2019-05-16

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Arkadiusz Bibillo ,  Pranav Patel

IPC: C12N9/12

CPC classification number: C12N9/1276 ,  C12Y207/07049 ,  C07K2319/20

Abstract: The present disclosure provides methods and systems for amplifying and analyzing nucleic acid samples. The present disclosure provides methods for preparing cDNA and/or DNA molecules ad cDNA and/or DNA libraries using modified reverse transcriptases.

公开(公告)号：US20230194517A1

公开(公告)日：2023-06-22

申请号：US18083131

申请日：2022-12-16

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Eli HEFNER

IPC: G01N33/543 ,  G01N21/64 ,  B01L3/00

CPC classification number: G01N33/54386 ,  G01N21/6428 ,  G01N21/6458 ,  B01L3/502761 ,  G01N2021/6439 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2200/0652

Abstract: Systems and methods for multiplex detection through measurement of localized fluorescence ratios are disclosed herein. This can include creating a plurality of capture structures that each include a detection portion that can couple with a target analyte and a stem that can include a capture structure code uniquely identifying a type of the capture structure. The capture structures can be attached to a sample surface and mixed with a sample containing a plurality of target analytes. A location and the capture structure code of each of the capture structures can be determined. A location at which a target analyte is bound to one of the capture structures can be identified, and the target analyte can be determined based on the capture structure code of the capture structure at the location at which the target analyte is bound to one of the capture structures.

公开(公告)号：US11666888B2

公开(公告)日：2023-06-06

申请号：US16967429

申请日：2019-02-05

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： Christopher Belisle ,  Hong Chen ,  Yueping Xu ,  Jiali Liao ,  Xuemei He

IPC: B01D15/32 ,  B01D15/36 ,  B01D15/38 ,  B01J20/289 ,  B01J20/32 ,  C07K16/06 ,  B01J20/26

CPC classification number: B01J20/262 ,  B01D15/363 ,  B01J20/289 ,  B01J20/3208 ,  B01J20/3219 ,  B01J20/3227 ,  B01J20/3248 ,  B01J20/3253 ,  B01J20/3285 ,  C07K16/065 ,  B01D15/327 ,  B01D15/3847

Abstract: Chromatography resins having mixed mode ligands and methods of using such resins are provided.

公开(公告)号：US20230152011A1

公开(公告)日：2023-05-18

申请号：US17989938

申请日：2022-11-18

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Amir SADRI ,  Nenad KIRCANSKI ,  Thanh-Vi TRAN ,  Carl MARLOWE ,  Brian David WILSON

IPC: F25B21/04 ,  B01L7/00 ,  H10N10/10 ,  H10N10/80

CPC classification number: F25B21/04 ,  B01L7/00 ,  B01L7/52 ,  H10N10/10 ,  H10N10/80 ,  B01L2300/1805 ,  B01L2300/08 ,  B01L2300/123

Abstract: A heat pump that includes a thermoelectric device(s) and a heat sink having a raised portion with a top surface for thermally coupling with a planar face of the thermoelectric device(s). The raised portion of the heat sink includes an outer periphery and a raised central region surrounded by a void region to provide more uniform thermal conductivity when clamped within an assembly. The raised central region is shaped in an any shape corresponding to a shape of uneven thermal conductivity due to clamping pressure applied to the heat sink. The void region can be substantially contiguous and entirely circumscribe the central raised region. The device can optionally include discrete supports formed of a less thermally-conductive material within the void region. The supports can be elastomeric, such as O-rings, and disposed within pockets defined within the void region.

公开(公告)号：US11639933B2

公开(公告)日：2023-05-02

申请号：US16141069

申请日：2018-09-25

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Boaz Gildor ,  Dalia Shezifi

IPC: G01N33/543 ,  G01N33/541

Abstract: Methods of detecting a target in a sample are provided. Kits for performing the methods described herein are also provided.

公开(公告)号：US20230127357A1

公开(公告)日：2023-04-27

申请号：US18046967

申请日：2022-10-17

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： CHRISTOPHER BELISLE ,  JIALI LIAO ,  CALLISTA KATRINA ASUNCION ,  EDWIN LOZANO

IPC: C07K1/16

Abstract: The subject invention pertains to mixed mode chromatography ligands and chromatography matrices suitable for the purification of proteins from biological sources or biological samples. Methods of making chromatography matrices comprising the disclosed ligands and using the disclosed chromatography matrices are also provided.

公开(公告)号：US11635365B2

公开(公告)日：2023-04-25

申请号：US17686229

申请日：2022-03-03

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique

IPC: G01N15/14 ,  B01L3/00 ,  C12M1/00 ,  G01N1/20 ,  G01N1/28 ,  G01N1/40 ,  G06V20/69 ,  B01L3/02 ,  G01N35/00 ,  G01N15/00 ,  G01N15/10

Abstract: A cell capture system including an array, an inlet manifold, and an outlet manifold. The array includes a plurality of parallel pores, each pore including a chamber and a pore channel, an inlet channel fluidly connected to the chambers of the pores; an outlet channel fluidly connected to the pore channels of the pores. The inlet manifold is fluidly connected to the inlet channel, and the outlet channel is fluidly connected to the outlet channel. A cell removal tool is also disclosed, wherein the cell removal tool is configured to remove a captured cell from a pore chamber.

公开(公告)号：US20230100295A1

公开(公告)日：2023-03-30

申请号：US18075009

申请日：2022-12-05

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Ronald Lebofsky

IPC: B01L3/00 ,  B01L7/00 ,  C12Q1/686

Abstract: The invention(s) cover systems and methods for target detection in a multiplexed and rapid manner. Embodiments of the system can include: a base substrate; and an array of sample processing regions defined at a broad surface of the base substrate, wherein each of the array of sample processing regions includes: a set of microwell subarrays arranged in a gradient by volumetric capacity between an upstream end and a downstream end of each respective sample processing region, and a boundary separating each respective sample processing region from adjacent sample processing regions. The system can support methods, with example implementation by an automated platform, for returning preliminary results from a subset of microwells of the samples processing regions, as well as results pertaining to specific and non-specific amplification, for multiple targets of a sample.

公开(公告)号：US20230086845A1

公开(公告)日：2023-03-23

申请号：US17992304

申请日：2022-11-22

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Jonathan William Larson ,  Qun Zhong ,  Darren Roy Link

IPC: C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/686

Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.

公开(公告)号：US11603556B2

公开(公告)日：2023-03-14

申请号：US17087952

申请日：2020-11-03

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald Lebofsky

IPC: C12Q1/68 ,  C12Q1/6834 ,  C12Q1/6855

Abstract: Methods and compositions for attaching cell-specific barcodes without formation of partitions is provided.