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    SINGLE-PARTICLE ANALYSIS OF PARTICLE POPULATIONS
    22.
    发明申请
    SINGLE-PARTICLE ANALYSIS OF PARTICLE POPULATIONS 有权
    颗粒人口的单粒子分析

    公开(公告)号：US20130323732A1

    公开(公告)日：2013-12-05

    申请号：US13899397

    申请日：2013-05-21

    Applicant: Fluidigm Corporation

    Inventor： Megan Anderson ,  Peilin Chen ,  Brian Fowler ,  Fiona Kaper ,  Ronald Lebofsky ,  Andrew May

    IPC: C12Q1/68 ,  B01D15/08

    CPC classification number: C12Q1/6813 ,  B01D15/08 ,  C12Q1/6806 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/629

    Abstract: In certain embodiments, the invention provides methods and devices for assaying single particles in a population of particles, wherein at least two parameters are measured for each particle. One or more parameters can be measured while the particles are in the separate reaction volumes. Alternatively or in addition, one or more parameters can be measured in a later analytic step, e.g., where reactions are carried out in the separate reaction volumes and the reaction products are recovered and analyzed. In particular embodiments, one or more parameter measurements are carried out “in parallel,” i.e., essentially simultaneously in the separate reaction volumes.

    Abstract translation: 在某些实施方案中，本发明提供用于测定颗粒群体中的单个颗粒的方法和装置，其中针对每个颗粒测量至少两个参数。 可以在颗粒处于分开的反应体积中时测量一个或多个参数。 或者或另外，可以在稍后的分析步骤中测量一个或多个参数，例如其中在分开的反应体积中进行反应并回收和分析反应产物。 在具体实施方案中，一个或多个参数测量“并行地进行”，即基本上同时在单独的反应体积中进行。

    Microfabricated fluidic circuit elements and applications
    25.
    发明申请
    Microfabricated fluidic circuit elements and applications 有权
    微型流体回路元件及应用

    公开(公告)号：US20020195152A1

    公开(公告)日：2002-12-26

    申请号：US09995397

    申请日：2001-11-26

    Applicant: Fluidigm Corporation

    Inventor： David N. Fernandes ,  Hou-Pu Chou ,  Marc A. Unger

    IPC: F15C001/00

    CPC classification number: F15C3/00 ,  A61M2206/22 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2200/0621 ,  B01L2200/14 ,  B01L2300/123 ,  B01L2400/0487 ,  B01L2400/0638 ,  B01L2400/0655 ,  F15C3/04 ,  F16K99/0001 ,  F16K99/0009 ,  F16K99/0015 ,  F16K99/0034 ,  F16K99/0059 ,  F16K2099/0074 ,  F16K2099/008 ,  F16K2099/0082 ,  F16K2099/0084 ,  G06F17/509 ,  G06F2217/16 ,  H01H2029/008 ,  Y10T137/206 ,  Y10T137/2202 ,  Y10T137/2218 ,  Y10T137/2224 ,  Y10T137/7879 ,  Y10T137/7891 ,  Y10T137/7892

    Abstract: The present invention provides microfabricated fluidic systems and methods. Microfabricated fluidic devices of the present invention include switches that can be opened and closed to allow or block the flow of fluid through a channel in response to the pressure level in a gate of the switch. The microfabricated fluidic switches may be coupled together to perform logic functions and Boolean algebra, such as inverters, AND gates, NAND, gates, NOR gates, and OR gates. The logic gates may be coupled together to form flip-flops that latch signals. The present invention also includes microfabricated fluidic pressure multipliers that increase the pressure in a second chamber relative to a first chamber. Microfabricated fluidic devices of the present invention also include pressure sources. A pressure source of the present includes a pump coupled to a reservoir through unidirectional valves. The pressure source may be high pressure source or a low pressure source. Microfabricated fluidic devices of the present invention may also include devices that perform analog functions such as switching regulator.

    Abstract translation: 本发明提供微制造流体系统和方法。 本发明的微型流体装置包括可以打开和关闭的开关，以响应于开关的门中的压力水平来允许或阻止流体通过通道的流动。 微制造的流体开关可以耦合在一起以执行逻辑功能和布尔代数，例如反相器，与门，NAND，门，NOR门和或门。 逻辑门可以耦合在一起以形成锁存信号的触发器。 本发明还包括相对于第一腔室增加第二腔室中的压力的​​微制造流体压力倍增器。 本发明的微型流体装置还包括压力源。 本发明的压力源包括通过单向阀联接到储存器的泵。 压力源可以是高压源或低压源。 本发明的微型流体装置还可以包括执行诸如开关调节器的模拟功能的装置。

    NUCLEIC ACID ENCODING REACTIONS
    28.
    发明申请
    NUCLEIC ACID ENCODING REACTIONS 有权

    公开(公告)号：US20220119874A1

    公开(公告)日：2022-04-21

    申请号：US17480067

    申请日：2021-09-20

    Applicant: Fluidigm Corporation

    Inventor： Megan Anderson ,  Peilin Chen ,  Brian Fowler ,  Robert C. Jones ,  Fiona Kaper ,  Ronald Lebofsky ,  Andrew May

    IPC: C12Q1/6855 ,  C12N15/65 ,  C12N15/66 ,  C12N15/10 ,  C12Q1/686 ,  C12Q1/6874

    Abstract: Described herein are methods useful for incorporating one or more adaptors and/or nucleotide tag(s) and/or barcode nucleotide sequence(s) one, or typically more, target nucleotide sequences. In particular embodiments, nucleic acid fragments having adaptors, e.g., suitable for use in high-throughput DNA sequencing are generated. In other embodiments, information about a reaction mixture is encoded into a reaction product. Also described herein are methods and kits useful for amplifying one or more target nucleic acids in preparation for applications such as bidirectional nucleic acid sequencing. In particular embodiments, methods of the invention entail additionally carrying out bidirectional DNA sequencing. Also described herein are methods for encoding and detecting and/or quantifying alleles by primer extension.

    METHOD FOR CAPTURING AND ENCODING NUCLEIC ACID FROM A PLURALITY OF SINGLE CELLS
    29.
    发明申请
    METHOD FOR CAPTURING AND ENCODING NUCLEIC ACID FROM A PLURALITY OF SINGLE CELLS 有权

    公开(公告)号：US20210254044A1

    公开(公告)日：2021-08-19

    申请号：US17065922

    申请日：2020-10-08

    Applicant: Fluidigm Corporation

    Inventor： Sten Linnarsson ,  Gioele Le Manno ,  Amit Zeisel

    IPC: C12N15/10

    Abstract: This invention relates to methods for capturing and encoding nucleic acid from a plurality of single cells. A plurality of solid supports is randomly placed into a plurality of compartments, such that the average number of solid supports per compartment, λ1, is less than 1, wherein each solid support carries (a) a unique identification sequence and (b) a capture moiety. A plurality of single cells is randomly placing into the plurality of compartments, such that the average number of cells per compartment, λ2, is less than 1. These random placement steps may be performed in any order. Nucleic acid is then released from each single cell and captured via the capture moiety, such that nucleic acid from each single cell is tagged with a unique identification sequence.

    METHOD AND SYSTEMS FOR MICROFLUIDIC LOGIC DEVICES
    30.
    发明申请
    METHOD AND SYSTEMS FOR MICROFLUIDIC LOGIC DEVICES 审中-公开

    公开(公告)号：US20190329253A1

    公开(公告)日：2019-10-31

    申请号：US16377029

    申请日：2019-04-05

    Applicant: Fluidigm Corporation

    Inventor： Naga Gopi Devaraju ,  Marc A. Unger

    IPC: B01L3/00 ,  F17D3/00 ,  G01N35/00

    Abstract: A microfluidic system includes a substrate, a set of input ports coupled to the substrate, and a set of output ports coupled to the substrate. The microfluidic system also includes a microfluidic processing system coupled to the substrate and including a plurality of processing sites. The microfluidic processing system is coupled to the set of input ports and the set of output ports. The microfluidic system further includes one or more microfluidic logic devices coupled to the substrate and operable to control at least a portion of the microfluidic processing system.

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