-
21.发明授权
公开(公告)号：US5650316A
公开(公告)日：1997-07-22
申请号：US254114
申请日：1994-06-06
IPC分类号： A61K38/00 , A61K48/00 , C12N15/113 , C12N5/10 , C07H21/04 , C12Q1/68 , G01N33/574
CPC分类号： A61K31/7088 , A61K31/7125 , A61K48/00 , C12N15/1136 , A61K38/00 , C12N2310/15 , C12N2310/315 , C12N2310/351 , C12N2310/3515
摘要： The present invention provides novel methods of treating anti-neoplastic and non-neoplastic cell proliferative diseases. The present methods involve administration of triplex forming oligonucleotides to humans to inhibit the biological activity of tumor necrosis factor. Also provided are methods of treating neuro-oncologic states and renal cancer.
摘要翻译： 本发明提供治疗抗肿瘤和非赘生性细胞增殖性疾病的新方法。 本发明的方法包括向人形成三链体形成寡核苷酸以抑制肿瘤坏死因子的生物学活性。 还提供了治疗神经肿瘤学状态和肾癌的方法。
-
公开(公告)号：US5532128A
公开(公告)日：1996-07-02
申请号：US353957
申请日：1994-12-12
申请人： Mitchell D. Eggers , Michael E. Hogan , Kenneth L. Beattie , John Shumaker , Daniel J. Ehrlich , Mark Hollis
发明人： Mitchell D. Eggers , Michael E. Hogan , Kenneth L. Beattie , John Shumaker , Daniel J. Ehrlich , Mark Hollis
IPC分类号： B01J19/00 , C12Q1/68 , C40B40/06 , C40B60/14 , G01N21/25 , G01N27/02 , G01N27/22 , G01N33/50 , G01N33/53 , G01N33/543 , G01N37/00
CPC分类号： B82Y30/00 , B01J19/0046 , B01J19/0093 , C12Q1/6825 , C12Q1/6832 , C12Q1/6837 , C12Q1/6874 , G01N21/253 , G01N27/221 , G01N27/226 , G01N33/54373 , G01N33/5438 , B01J2219/00317 , B01J2219/00511 , B01J2219/00527 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00653 , B01J2219/00659 , B01J2219/00702 , B01J2219/00711 , B01J2219/00713 , B01J2219/00722 , C40B40/06 , C40B60/14 , Y10S436/806 , Y10S436/809
摘要： A method and apparatus for identifying molecular structures within a sample substance using an array having a plurality of test sites upon which the sample substance is applied. Each test site includes a probe formed therein to bond with an associated target molecular structure. An electrical signal is applied to the test site and the electrical properties of the test sites are detected to determine which probes have bonded to an associated target molecular structure.
-
公开(公告)号：US10272409B2
公开(公告)日：2019-04-30
申请号：US12080720
申请日：2008-04-04
申请人： Michael E. Hogan , Sergy Lemeshko , Yuri Belosludtsev , Thomas F. Powdrill , Rahul Mitra , Joseph G. Utermohlen , Frederick H. Eggers
发明人： Michael E. Hogan , Sergy Lemeshko , Yuri Belosludtsev , Thomas F. Powdrill , Rahul Mitra , Joseph G. Utermohlen , Frederick H. Eggers
摘要： Provided herein are biomolecular hybridization devices comprising a substrate with a permanently and covalently attached surface of functional groups and an adsorbed monolayer of unmodified, single-stranded oligonucleotides all of which are 10 to about 24 bases in length as a saturated film of constrained oligonucleotides on the surface via direct non-covalent phosphate-surface adsorptive contact of substantially all phosphate groups of each oligonucleotide. The constrained oligonucleotides are effective to dissociably hybridize to a complementary single-stranded nucleic acid with asymmetric, non-helical base pairing and without oligonucleotide dissociation from the surface of the device. Also, provided are methods for hybridizing solution-state target nucleic acids to probe nucleic acids and for identifying a nucleotide sequence to which a nucleotide-binding protein binds using the biomolecular hybridization devices.
-
公开(公告)号：US20120302457A1
公开(公告)日：2012-11-29
申请号：US13476774
申请日：2012-05-21
CPC分类号： C12Q1/6837 , C12Q1/6881 , C12Q2600/156
摘要： The present invention provides a portable system for real-time population-scale HLA genotyping and/or allelotyping in a field environment and methods of such population-scale HLA genotyping. The individual components of the system are portable to and operable within a field environment thereby providing high throughput with real-time geno- or allelotyping. Also provided are HLA gene-specific primers and HLA allele-specific or single nucleotide polymorphism-specific hybridization probes. In addition the present invention provides a microarray comprising the hybridization probes. Further provided is a kit comprising the HLA gene-specific primers and the microarray.
摘要翻译： 本发明提供了一种用于现场环境中实时种群规模HLA基因分型和/或等位基因的便携式系统以及这种群体规模HLA基因分型的方法。 系统的各个组件可以在现场环境中移植到并可操作，从而通过实时基因或等位基因提供高吞吐量。 还提供了HLA基因特异性引物和HLA等位基因特异性或单核苷酸多态性特异性杂交探针。 此外，本发明提供了包含杂交探针的微阵列。 还提供了包含HLA基因特异性引物和微阵列的试剂盒。
-
公开(公告)号：US06355785B1
公开(公告)日：2002-03-12
申请号：US09429130
申请日：1999-10-28
申请人： Robert F. Rando , Susan Fennewald , Joseph G. Zendegui , Joshua O. Ojwang , Michael E. Hogan , Yves Pommier , Abhijit Mazumder
发明人： Robert F. Rando , Susan Fennewald , Joseph G. Zendegui , Joshua O. Ojwang , Michael E. Hogan , Yves Pommier , Abhijit Mazumder
IPC分类号： C07H2100
CPC分类号： A61K31/70 , A61K31/711 , A61K31/7115 , A61K31/7125 , A61K38/00 , C07H21/00 , C12N15/1132 , C12N15/1133 , C12N2310/151 , C12N2310/18 , C12N2310/315 , C12N2310/321 , C12N2310/33 , C12N2310/335 , C12N2310/351 , C12N2310/3515 , H01L23/293 , H01L2924/0002 , C12N2310/3521 , H01L2924/00
摘要： A method and compositions for treating viral infection (IV) vitro and in vivo using a guanosine-rich oligonucleotide. The oligonucleotides have sufficient guanosine to form a guanosine tetrad. Also provided are oligonucleotides of at least two runs of at least two guanosines. Also provided are guanosine-rich oligonucleotides and methods for treating viral infections in humans, and a method for designing guanosine-rich oligonucleotides having anti-viral activity and integrase inhibition activity.
摘要翻译： 一种使用富含鸟苷酸的寡核苷酸来体外和体内治疗病毒感染（IV）的方法和组合物。 寡核苷酸具有足够的鸟苷以形成鸟苷四肽。 还提供了至少两次至少两种鸟苷的寡核苷酸。 还提供了富含鸟苷酸的寡核苷酸和用于治疗人类病毒感染的方法，以及设计具有抗病毒活性和整合酶抑制活性的富含鸟嘌呤核苷酸的方法。
-
26.发明授权公开(公告)号：US06323185B1
公开(公告)日：2001-11-27
申请号：US08682255
申请日：1996-07-17
IPC分类号： A61K3170
CPC分类号： A61K31/70 , A61K31/711 , A61K31/7115 , A61K31/7125 , A61K38/00 , C07H21/00 , C12N15/1132 , C12N15/1133 , C12N2310/151 , C12N2310/18 , C12N2310/315 , C12N2310/321 , C12N2310/33 , C12N2310/335 , C12N2310/351 , C12N2310/3515 , H01L23/293 , H01L2924/0002 , C12N2310/3521 , H01L2924/00
摘要： A method and compositions for treating viral infection in vitro and in vivo using a guanosine-rich oligonucleotide. The oligonucleotides have sufficient guanosine to form a guanosine tetrad. Also provided are oligonucleotides of at least two runs of at least two guanosines. Also provided are guanosine-rich oligonucleotides and methods for treating viral infections in humans, and a method for designing guanosine-rich oligonucleotides having anti-viral activity and integrase inhibition activity.
摘要翻译： 一种使用富含鸟苷酸的寡核苷酸在体外和体内治疗病毒感染的方法和组合物。 寡核苷酸具有足够的鸟苷以形成鸟苷四肽。 还提供了至少两次至少两种鸟苷的寡核苷酸。 还提供了富含鸟苷酸的寡核苷酸和用于治疗人类病毒感染的方法，以及设计具有抗病毒活性和整合酶抑制活性的富含鸟嘌呤核苷酸的方法。
-
公开(公告)号：US06288042B1
公开(公告)日：2001-09-11
申请号：US09017974
申请日：1998-02-03
IPC分类号： A61K3170
CPC分类号： A61K31/70 , A61K38/00 , C07H21/00 , C12N15/1132 , C12N2310/151 , C12N2310/18 , C12N2310/315 , C12N2310/335
摘要： Guanosine-rich oligonucleotides having sequences that favor the formation under physiological conditions of a stable four-stranded structure containing two stacked guanosine quartets (G4s) are disclosed. These oligonucleotides demonstrate enhanced nuclease resistance, cellular uptake and biological efficacy. Methods and composition for treating viral infection using these guanosine-rich oligonucleotides are also disclosed. Certain embodiments of the new oligonucleotides are 16-17 nucleotides long and contain at least one C-5 propynyl dU substitution. A method for designing anti-viral oligonucleotides is also disclosed.
摘要翻译： 公开了具有有利于在包含两个堆叠鸟苷四重奏（G4）的稳定的四链结构的生理条件下形成的序列的富含鸟苷酸的寡核苷酸。 这些寡核苷酸表现出增强的核酸酶抗性，细胞摄取和生物功效。 还公开了使用这些富含鸟嘌呤的寡核苷酸治疗病毒感染的方法和组合物。 新的寡核苷酸的某些实施方案长16-17个核苷酸并且含有至少一个C-5丙炔基dU取代。 还公开了设计抗病毒寡核苷酸的方法。
-
公开(公告)号：US6150339A
公开(公告)日：2000-11-21
申请号：US987574
申请日：1997-12-09
CPC分类号： A61K31/70 , A61K31/711 , A61K31/7115 , A61K31/7125 , C07H21/00 , C12N15/1132 , C12N15/1133 , A61K38/00 , C12N2310/151 , C12N2310/18 , C12N2310/315 , C12N2310/321 , C12N2310/33 , C12N2310/335 , C12N2310/351 , C12N2310/3515
摘要： A method and compositions for treating viral infection in vitro and in vivo using a guanosine-rich oligonucleotides. The oligonucleotides have sufficient guanosine to form a guanosine tetrad. They can be composed of at least about 40% guanosine nucleotides. Also provided are oligonucleotides of at least two runs of at least two guanosines. Also provided are guanosine-rich oligonucleotides and methods for treating viral infections in humans, and a method for designing guanosine-rich oligonucleotides having anti-viral activity.
摘要翻译： 一种用于使用富含鸟苷的寡核苷酸在体外和体内治疗病毒感染的方法和组合物。 寡核苷酸具有足够的鸟苷以形成鸟苷四肽。 它们可以由至少约40％的鸟苷核苷酸组成。 还提供了至少两次至少两种鸟苷的寡核苷酸。 还提供了富含鸟苷的寡核苷酸和用于治疗人类病毒感染的方法，以及设计具有抗病毒活性的富含鸟苷酸的寡核苷酸的方法。
-
公开(公告)号：US5614503A
公开(公告)日：1997-03-25
申请号：US467114
申请日：1995-06-06
IPC分类号： A61K9/127 , A61K48/00 , C07J41/00 , C07C261/00 , C07J9/00
CPC分类号： C07J41/0055 , A61K48/00 , A61K9/1272
摘要： A nucleic acid transporter to deliver a nucleic acids into cells, comprising a cationic compound having a cationic head group for binding the nucleic acid and a lipid tail for association with the membrane. A cationic compound usually is a polyamine or a short basic peptide. The lipid tail is usually selected from the group consisting of plant steroid, animal steroid, isoprenoid compound, aliphatic lipid, pore forming protein, pore forming peptides and fusogenic peptides. The cationic head and the lipid tail are linked through a carbamate linkage. When polyamine is used, it is preferably either spermidine or spermine and the nucleic acid can be any of a variety, including triplex forming oligonucleotides, antisense oligonucleotide, aptamers, ribozymes, plasmids and DNA for gene therapy. Also described is a method for treating individuals using the transporter linked to a therapeutic nucleic acid.
摘要翻译： 将核酸递送到细胞中的核酸转运蛋白，其包含具有用于结合核酸的阳离子头基的阳离子化合物和与所述膜缔合的脂质尾。 阳离子化合物通常是多胺或短碱性肽。 脂质尾巴通常选自植物类固醇，动物类固醇，类异戊二烯化合物，脂肪族脂质，成孔蛋白，成孔肽和融合肽。 阳离子头和脂质尾巴通过氨基甲酸酯键连接。 当使用多胺时，其优选为亚精胺或精胺，核酸可为任何多种，包括形成三链体的寡核苷酸，反义寡核苷酸，适体，核酶，质粒和用于基因治疗的DNA。 还描述了使用与治疗性核酸连接的转运蛋白来治疗个体的方法。
-
30.发明授权Method for making synthetic oligonucleotides which bind specifically to target sites on duplex DNA molecules, by forming a colinear triplex, the synthetic oligonucleotides and methods of use 失效通过形成共线三联制备合成寡核苷酸的方法，其特异性结合双链DNA分子上的靶位点，合成寡核苷酸和使用方法
公开(公告)号：US5176996A
公开(公告)日：1993-01-05
申请号：US453532
申请日：1989-12-22
CPC分类号： C12N15/113 , A61K48/00 , C07H21/00 , C07H21/04 , C12N15/1137 , C12N15/1138 , C12Q1/68 , C12Q1/6839 , C12Q1/6876 , C12Q1/6883 , C12Q1/6886 , C12Q1/703 , C12Q1/705 , C12Q1/708 , C12Y101/01034 , C12Y205/01018 , C12Y207/07007 , A61K38/00 , C12N2310/15 , C12N2310/321 , Y10T436/143333
摘要： A method for making synthetic oligonucleotides which bind to target sequences in a duplex DNA forming colinear triplexes by binding to the major groove. The method includes scanning genomic duplex DNA and identifying nucleotide target sequences of greater than about 20 nucleotides having either about at least 65% purine bases or about at least 65% pyrimidine bases; and synthesizing synthetic oligonucleotides complementary to identified target sequences. The synthetic oligonucleotides have a G when the complementary location in the DNA duplex has a GC base pair and have a T when the complementary location in the DNA duplex has an AT base pair. The synthetic oligonucleotides are oriented 5' to 4' and bind parallel or 3' to 5' and bind anti-parallel to the about at least 65% purine strand.
摘要翻译： 一种制备合成寡核苷酸的方法，其通过结合主要沟槽结合形成共线三联体的双链体DNA中的靶序列。 该方法包括扫描基因组双链DNA并鉴定大于约20个核苷酸的核苷酸靶序列，其具有约至少65％的嘌呤碱基或约至少65％的嘧啶碱基; 并合成与鉴定的靶序列互补的合成寡核苷酸。 当DNA双链体中的互补位置具有GC碱基对并且当DNA双链体中的互补位置具有AT碱基对时，合成的寡核苷酸具有G。 合成的寡核苷酸定向为5'至4'，并且平行或3'至5'结合并与大约至少65％的嘌呤链反平行结合。
-
-
-
-
-
-
-
-
-
搜索结果
46 条记录 (耗时 0.057 秒)
