公开(公告)号：US06428702B1

公开(公告)日：2002-08-06

申请号：US09918435

申请日：2001-08-01

申请人： Terry A. Berger ,  Kimber D. Fogelman

发明人： Terry A. Berger ,  Kimber D. Fogelman

IPC分类号： B01D1100

CPC分类号： B01D15/14 ,  B01D15/40 ,  G01N30/02 ,  G01N30/16

摘要： An improved method and apparatus for safely introducing sample into a flow stream of a supercritical fluid chromatography system is described. The sample injection method improves safety of injection by eliminating the risk of injecting sample into a high-pressure system containing liquids and gasses at or near supercritical ranges. The method improves the efficiency and significantly shortens the time of the injection process by using a check valve placed between a fill port on an injection valve and sample port where sample is injected by a syringe. After sample injection from a sample loop into a high-pressure flow stream, an injection valve is reset to receive a subsequent sample. High-pressure flow stream mixtures become trapped within the sample loop when the valve is reset. The check valve prevents the mixtures from expanding into the sample port and blowing out a syringe and flow stream mixtures from the fill lines. The trapped flow stream mixtures are directed to a waste port where they are safely contained. The technique can be applied equally as effective to preparatory SFC as well as to analytical SFC systems.

摘要翻译： 描述了用于将样品安全地引入超临界流体色谱系统的流动流的改进的方法和装置。 样品注射方法通过消除在超临界范围内或附近含有液体和气体的高压系统中注入样品的风险，提高了注射的安全性。 该方法通过使用放置在注射阀上的填充口和通过注射器注射样品的样品端口的样品端口之间的止回阀来提高效率并显着缩短注射过程的时间。 从样品回路进样到高压流动流中后，重新注入进样阀以接收随后的样品。 当阀门复位时，高压流动流混合物将被捕获在样品回路内。 止回阀防止混合物膨胀到样品端口，吹出注射器并从填充线流出混合物。 被捕获的流动流混合物被引导到废物端口，在那里它们被安全地包含。 该技术可以同样适用于预备性证监会以及分析SFC系统。

公开(公告)号：US10279294B2

公开(公告)日：2019-05-07

申请号：US14123191

申请日：2012-06-01

申请人： Kimber D Fogelman ,  Terry A Berger ,  Edwin E Wikfors

发明人： Kimber D Fogelman ,  Terry A Berger ,  Edwin E Wikfors

IPC分类号： B01D46/00 ,  B01D11/02 ,  B01D11/04 ,  B01D15/24 ,  B01D15/40 ,  G01N30/80 ,  G01N30/84

摘要： An apparatus, process, and system are disclosed that effectively provide separation of a combined gas/liquid flow stream into its separated gas and liquid factions. The invention is primarily directed to the fields of preparative supercritical fluid chromatography (SFC) and supercritical fluid extraction (SFE), but will have other utilization and applicability where phases of dramatically different density, viscosity and volumetric flow require separation.

公开(公告)号：US09395109B2

公开(公告)日：2016-07-19

申请号：US13699804

申请日：2010-05-26

申请人： Terry A. Berger ,  Kimber Fogelman

发明人： Terry A. Berger ,  Kimber Fogelman

IPC分类号： F25B21/02 ,  F04B39/06 ,  F04B53/08 ,  B01D15/40 ,  G01N30/32

CPC分类号： F25B21/04 ,  B01D15/12 ,  B01D15/40 ,  F04B39/064 ,  F04B53/08 ,  F25B21/02 ,  G01N2030/326

摘要： A system for chilling a pumping system includes a Peltier cooling element, and first and second heat exchangers. The Peltier element is arranged near a pump head of a pump such that the Peltier element and the pump head are in thermal contact, where the Peltier element chills the pump head and a mobile phase flowstream prior to fluid from the mobile phase flowstream entering the pump. The first heat exchanger is attached to the Peltier element, and removes heat from the Peltier element using a circulating fluid. The second heat exchanger cools the circulating fluid, where the Peltier element and the pump head are arranged in an area that is isolated from the second heat exchanger.

摘要翻译： 用于冷却泵送系统的系统包括珀尔帖冷却元件和第一和第二热交换器。 珀尔帖元件设置在泵的泵头附近，使得珀尔帖元件和泵头处于热接触状态，其中珀尔帖元件在流入流入泵的流动相流动之前，使泵头和流动相流动流动 。 第一个热交换器连接到珀尔帖元件，并使用循环流体从珀尔帖元件中除去热量。 第二热交换器冷却循环流体，其中珀耳帖元件和泵头被布置在与第二热交换器隔离的区域中。

公开(公告)号：US20130048095A1

公开(公告)日：2013-02-28

申请号：US13530931

申请日：2012-06-22

申请人： Edwin E. WIKFORS ,  Kimber D. FOGELMAN ,  Terry A. BERGER

发明人： Edwin E. WIKFORS ,  Kimber D. FOGELMAN ,  Terry A. BERGER

IPC分类号： F16K17/00 ,  F17D3/00

CPC分类号： F04B11/0075 ,  F04B23/06 ,  G01N30/32 ,  G01N30/34 ,  G01N2030/326 ,  Y10T137/0352 ,  Y10T137/7722 ,  G01N2030/342 ,  B01D15/40

摘要： An apparatus, system, and process of converting a standard, high performance liquid chromatography (HPLC) flow path to a flow path suitable for supercritical fluid chromatography (SFC) are described. This reversible technique is applied to a variety of flow configurations including binary, high pressure solvent mixing systems and quaternary, low pressure solvent mixing systems than can be conventionally operated or automated. The technique is generally applied to the fields of supercritical fluid chromatography and high pressure liquid chromatography, but users skilled in the art will find utility for any flow system where pressurization components must be periodically applied to and removed from both ends of a flow stream in an automated manner.

摘要翻译： 描述了将标准的高效液相色谱（HPLC）流动路径转换到适用于超临界流体色谱（SFC）的流动路径的装置，系统和方法。 这种可逆技术应用于各种流动配置，包括二元，高压溶剂混合系统和四级，低压溶剂混合系统，比常规操作或自动化。 该技术通常应用于超临界流体色谱和高压液相色谱领域，但是本领域技术人员将发现对于任何流动系统都将发挥效用，其中加压组分必须周期性地施加到流动流的两端并从其中移除 自动化方式。

公开(公告)号：US06652753B2

公开(公告)日：2003-11-25

申请号：US10067011

申请日：2002-02-04

申请人： Terry A. Berger ,  Kimber D. Fogelman ,  L. Thompson Staats ,  Mark Nickerson ,  Paul Bente ,  Kenneth J. Klein

发明人： Terry A. Berger ,  Kimber D. Fogelman ,  L. Thompson Staats ,  Mark Nickerson ,  Paul Bente ,  Kenneth J. Klein

IPC分类号： C02F144

CPC分类号： B01D15/247 ,  B01D11/0203 ,  B01D15/40 ,  G01N1/405 ,  G01N30/24 ,  G01N30/82

摘要： A fractionated sample collection process and device having at least one pressurized chamber used to gather and store liquid samples generated from a flow stream containing a mixture of highly compressed gas, compressible liquid, or supercritical fluid and a relatively incompressible liquid. A bank of multiple collection chambers is secured together in a frame to form a cassette unit. Each collection chamber may house a replaceable liner such as a test tube vial, to hold a fractionated liquid phase sample. After filling with sample, a collection chamber is returned to a clean state and ready for refilling with a new sample by manually or automatically replacing the liner.

公开(公告)号：US4982597A

公开(公告)日：1991-01-08

申请号：US377534

申请日：1989-07-10

申请人： Terry A. Berger

发明人： Terry A. Berger

IPC分类号： G01N30/02 ,  G01N30/30 ,  G01N30/60 ,  G01N30/68

CPC分类号： G01N30/68 ,  G01N30/30 ,  G01N2030/3015 ,  G01N2030/3038 ,  G01N2030/6008 ,  G01N2030/6013 ,  G01N30/02

摘要： In a system for analyzing a chemical sample by chromatographic separation of said sample into components a method and apparatus are provided for heating the mobile phase to a desired temperature prior to exiting the restrictor, while maintaining the density of the mobile phase at a desired level. Such heating and density maintenance being provided by the combination of a restrictor having a minimal taper length and a temperature zone immediately prior to the outlet end of the restrictor, which temperature zone has a minimum length. A second temperature zone spaced upstream from the first zone, and an intermediate thermally conductive member between the first and second zones can be provided as an alternative embodiment for establishing a thermal gradient. As the mobile phase passes through the second zone, the intermediate conductive member, and the first zone, the mobile phase is subjected to a thermal gradient.

摘要翻译： 在用于通过将所述样品色谱分离成组分来分析化学样品的系统中，提供了一种方法和装置，用于在将流动相的密度保持在期望水平之前将流动相加热至所需温度，然后再离开限流器。 这种加热和密度维持是通过具有最小锥度长度的限流器和紧邻限流器出口端之前的温度区域的组合提供的，该温度区域具有最小长度。 作为建立热梯度的替代实施例，可以提供在第一区域上游隔开的第二温度区域和位于第一区域和第二区域之间的中间导热构件。 当流动相通过第二区时，中间导电构件和第一区，流动相受到热梯度的影响。

公开(公告)号：US4845985A

公开(公告)日：1989-07-11

申请号：US157344

申请日：1988-02-17

申请人： Terry A. Berger

发明人： Terry A. Berger

IPC分类号： G01N30/02 ,  B01D11/00 ,  G01N30/28 ,  G01N30/30 ,  G01N30/32

CPC分类号： G01N30/30 ,  G01N30/28 ,  G01N2030/3038 ,  G01N2030/3076 ,  G01N2030/324 ,  G01N30/02

摘要： In a system for analyzing a chemical sample by chromatographic separation of said sample into components a method and apparatus are provided for maximizing detector resolution and minimizing duration by maintaining the linear velocity of the mobile phase at a desired level by affecting the viscosity and density characteristics of the mobile phase as it passes through a restrictor by programming the restrictor temperature.

公开(公告)号：US20140190183A1

公开(公告)日：2014-07-10

申请号：US13699804

申请日：2010-05-26

申请人： Terry A. Berger ,  Kimber Fogelman

发明人： Terry A. Berger ,  Kimber Fogelman

IPC分类号： F25B21/02

CPC分类号： F25B21/04 ,  B01D15/12 ,  B01D15/40 ,  F04B39/064 ,  F04B53/08 ,  F25B21/02 ,  G01N2030/326

摘要： Methods and systems for pumping compressible fluids in high pressure applications such as high-pressure liquid chromatography (HPLC) or supercritical fluid chromatography (SFC) applications are disclosed. An improved cooling device for a pump head for use in a supercritical fluid chromatography (SFC) system is described. A system for chilling a pumping system, includes a Peltier cooling element in thermal contact with a pump head, wherein the cooling element chills the pump head and a mobile phase fluid flowstream prior to the mobile phase fluid entering the pump; a fluid-cooled heat exchanger, attached to the Peltier cooling element, which removes heat from the cooling element using a circulating fluid; and a second heat exchanger which cools the circulating fluid.

摘要翻译： 公开了用于在高压液相色谱（HPLC）或超临界流体色谱（SFC）应用中的高压应用中泵送可压缩流体的方法和系统。 描述了用于超临界流体色谱（SFC）系统的用于泵头的改进的冷却装置。 用于冷却泵送系统的系统包括与泵头热接触的珀尔帖冷却元件，其中在移动相流体进入泵之前，冷却元件使泵头和流动相流体流动冷却; 附接到珀尔帖冷却元件的流体冷却的热交换器，其使用循环流体从冷却元件移除热量; 以及冷却循环流体的第二热交换器。

公开(公告)号：US20110233299A1

公开(公告)日：2011-09-29

申请号：US12730223

申请日：2010-03-23

申请人： Terry A. Berger ,  Samuel Colgate ,  Michael Casale

发明人： Terry A. Berger ,  Samuel Colgate ,  Michael Casale

IPC分类号： B05B15/00

CPC分类号： B01D15/10 ,  B01D15/163 ,  G01N30/02 ,  G01N30/28 ,  Y10T137/7904 ,  B01D15/40

摘要： A drive mechanism for back pressure regulator used in liquid chromatography, supercritical fluid chromatography, or supercritical fluid extraction allows very fine automated control over a very wide range of pressures by combining a linear actuator compressing a spring, pushing a pin. The nozzle assembly of the regulator comprises a flow through chamber containing a diaphragm and a seat, in which the pin pushes the diaphragm against the seat, together with an upstream pressure sensor and electronic feedback control to the motor of the actuator. The BPR of the embodiments exhibits high pressure stability and extremely low pressure noise, even at moderate to high pressures. The exemplary BPR can be use at either constant pressure or to generate pressure programs where the pressure is varied versus time. Further, the nozzle assembly has a field-replaceable head, requiring no mechanical adjustment on replacement.

摘要翻译： 用于液相色谱，超临界流体色谱或超临界流体萃取的背压调节器的驱动机构通过组合压缩弹簧的线性致动器，推动销，可以在非常宽的压力范围内进行非常精细的自动化控制。 调节器的喷嘴组件包括流通室，其包含隔膜和座，其中销将隔膜压靠在座上，以及上游压力传感器和电子反馈控制到致动器的电动机。 实施例的BPR表现出高压稳定性和极低压力噪声，即使在中等至高压下。 示例性BPR可以在恒定压力下使用或产生压力程序，其中压力随时间变化。 此外，喷嘴组件具有现场可更换头部，在更换时不需要机械调节。

公开(公告)号：US06685828B2

公开(公告)日：2004-02-03

申请号：US10119529

申请日：2002-04-09

申请人： Terry A. Berger ,  Kimber D. Fogelman ,  Kenneth Klein ,  L. Thompson Staats, III ,  Mark Nickerson ,  Paul F. Bente, III

发明人： Terry A. Berger ,  Kimber D. Fogelman ,  Kenneth Klein ,  L. Thompson Staats, III ,  Mark Nickerson ,  Paul F. Bente, III

IPC分类号： B01D1508

CPC分类号： B01D11/0203 ,  B01D15/247 ,  B01D15/40 ,  G01N30/24 ,  G01N30/82

摘要： A system for collection of liquid phase sample fractions from a supercritical fluid chromatography system is disclosed. The system has different ways of gathering the liquid phase into a collection chamber and provides for automated removal of liquid phase from a collection chamber into separate collection container. A separate pressure scheme is invoked upon the collection chambers to move liquid phase out of a chamber and into the separate collection container. In addition, the invention provides a renewal system for the collection chambers where each chamber returns to an uncontaminated stated by washing with a cleaning solution after removal of collected liquid phase. Spent cleaner from a chamber is purged using the pressure system into a waste stream for disposal.

摘要翻译： 公开了一种用于从超临界流体色谱系统收集液相样品馏分的系统。 该系统具有将液相收集到收集室中并且提供将液相从收集室自动移除到单独的收集容器中的不同方式。 在收集室上引用单独的压力方案以将液相移出室并进入单独的收集容器。 此外，本发明提供了一种用于收集室的更新系统，其中每个室在去除收集的液相后通过用清洁溶液洗涤而返回到未污染的状态。 使用压力系统将来自室的废清洁剂清洗成废物流以便处理。