公开(公告)号：US20240280569A1

公开(公告)日：2024-08-22

申请号：US18653506

申请日：2024-05-02

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Michael L. Samuels ,  Darren Roy Link

IPC: G01N33/543 ,  B01F33/3011 ,  B01F33/302 ,  C12Q1/6874 ,  C40B30/04 ,  C40B40/00 ,  C40B40/04 ,  C40B70/00 ,  G01N33/532

CPC classification number: G01N33/54366 ,  C12Q1/6874 ,  C40B40/00 ,  C40B40/04 ,  C40B70/00 ,  G01N33/532 ,  B01F33/3011 ,  B01F33/3021 ,  C40B30/04

Abstract: The invention generally relates to performing sandwich assays in droplets. In certain embodiments, the invention provides methods for detecting a target analyte that involve forming a compartmentalized portion of fluid including a portion of a sample suspected of containing a target analyte and a sample identifier, a first binding agent having a target identifier, and a second binding agent specific to the target analyte under conditions that produce a complex of the first and second binding agents with the target analyte, separating the complexes, and detecting the complexes, thereby detecting the target analyte.

公开(公告)号：US11898193B2

公开(公告)日：2024-02-13

申请号：US16565773

申请日：2019-09-10

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Benjamin J. Miller ,  Qun Zhong ,  Darren Roy Link

IPC: C12Q1/6806 ,  B05B1/08 ,  B01L3/00 ,  B05B1/26 ,  B05B7/00 ,  B01F23/41 ,  B01F33/3011 ,  B01F35/221 ,  B05B1/02 ,  B01L7/00

CPC classification number: C12Q1/6806 ,  B01F23/41 ,  B01F33/3011 ,  B01F35/2213 ,  B01L3/502761 ,  B01L3/502784 ,  B05B1/02 ,  B05B1/08 ,  B05B1/26 ,  B05B7/0012 ,  B01L7/525 ,  B01L2200/025 ,  B01L2200/0647 ,  B01L2200/141 ,  B01L2200/143 ,  B01L2200/148 ,  B01L2300/0867 ,  B01L2300/14 ,  B01L2400/0487 ,  Y10T436/2575

Abstract: The invention generally relates to methods and systems for manipulating droplet size. In certain aspects, the invention provides methods for manipulating droplet size that include forming droplets of aqueous fluid surrounded by an immiscible carrier fluid, and manipulating droplet size during the forming step by adjusting pressure exerted on the aqueous fluid or the carrier fluid.

公开(公告)号：US20230408505A1

公开(公告)日：2023-12-21

申请号：US18354144

申请日：2023-07-18

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Michael L. Samuels ,  Darren Roy Link

IPC: G01N33/543 ,  C40B40/04 ,  C40B70/00 ,  G01N33/532 ,  C40B40/00 ,  C12Q1/6874

CPC classification number: G01N33/54366 ,  C40B40/04 ,  C40B70/00 ,  G01N33/532 ,  C40B40/00 ,  C12Q1/6874 ,  B01F33/3011

Abstract: The invention generally relates to performing sandwich assays in droplets. In certain embodiments, the invention provides methods for detecting a target analyte that involve forming a compartmentalized portion of fluid including a portion of a sample suspected of containing a target analyte and a sample identifier, a first binding agent having a target identifier, and a second binding agent specific to the target analyte under conditions that produce a complex of the first and second binding agents with the target analyte, separating the complexes, and detecting the complexes, thereby detecting the target analyte.

公开(公告)号：US20230393069A1

公开(公告)日：2023-12-07

申请号：US18448631

申请日：2023-08-11

Applicant: Bio-Rad Laboratories, inc.

Inventor： Darren Roy Link ,  Michael L. Samuels

IPC: G01N21/64 ,  B01J19/00 ,  B01L3/00 ,  C40B40/04 ,  C40B50/08 ,  C40B60/10 ,  G01N33/50 ,  C12Q1/6851 ,  B01F23/41 ,  B01F25/433 ,  B01F33/3011 ,  C12Q1/25 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  G01N33/542 ,  G01N33/573 ,  G01N33/58

CPC classification number: G01N21/6445 ,  B01J19/0046 ,  B01L3/502761 ,  C40B40/04 ,  C40B50/08 ,  C40B60/10 ,  G01N33/5008 ,  C12Q1/6851 ,  B01F23/41 ,  B01F25/433 ,  B01F25/4336 ,  B01F33/3011 ,  C12Q1/25 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  G01N33/542 ,  G01N33/573 ,  G01N33/582 ,  B01L7/52

Abstract: The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.

公开(公告)号：US20230280337A1

公开(公告)日：2023-09-07

申请号：US18305650

申请日：2023-04-24

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Michael L. Samuels ,  Darren Roy Link

IPC: G01N33/543 ,  C40B40/04 ,  C40B70/00 ,  G01N33/532 ,  C40B40/00 ,  C12Q1/6874

CPC classification number: G01N33/54366 ,  C40B40/04 ,  C40B70/00 ,  G01N33/532 ,  C40B40/00 ,  C12Q1/6874 ,  B01F33/3011

Abstract: The invention generally relates to performing sandwich assays in droplets. In certain embodiments, the invention provides methods for detecting a target analyte that involve forming a compartmentalized portion of fluid including a portion of a sample suspected of containing a target analyte and a sample identifier, a first binding agent having a target identifier, and a second binding agent specific to the target analyte under conditions that produce a complex of the first and second binding agents with the target analyte, separating the complexes, and detecting the complexes, thereby detecting the target analyte.

公开(公告)号：US20210262020A1

公开(公告)日：2021-08-26

申请号：US17174519

申请日：2021-02-12

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Darren Roy Link

IPC: C12Q1/6848 ,  G01N1/38 ,  C12Q1/686 ,  B01J19/00 ,  B01F13/00 ,  B01L3/00 ,  B03C5/00 ,  B03C5/02 ,  B01F3/08 ,  C12Q1/00 ,  C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6874 ,  G01N35/08 ,  G01N15/14 ,  C12N15/10 ,  C12Q1/6827

Abstract: The invention generally relates to assemblies for displacing droplets from a vessel that facilitate the collection and transfer of the droplets while minimizing sample loss. In certain aspects, the assembly includes at least one droplet formation module, in which the module is configured to form droplets surrounded by an immiscible fluid. The assembly also includes at least one chamber including an outlet, in which the chamber is configured to receive droplets and an immiscible fluid, and in which the outlet is configured to receive substantially only droplets. The assembly further includes a channel, configured such that the droplet formation module and the chamber are in fluid communication with each other via the channel. In other aspects, the assembly includes a plurality of hollow members, in which the hollow members are channels and in which the members are configured to interact with a vessel. The plurality of hollow members includes a first member configured to expel a fluid immiscible with droplets in the vessel and a second member configured to substantially only droplets from the vessel. The assembly also includes a main channel, in which the second member is in fluid communication with the main channel. The assembly also includes at least one analysis module connected to the main channel.

公开(公告)号：US20210041432A1

公开(公告)日：2021-02-11

申请号：US16937908

申请日：2020-07-24

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Michael L. Samuels ,  Darren Roy Link

IPC: G01N33/543 ,  C40B40/04 ,  C40B70/00 ,  G01N33/532 ,  C40B40/00 ,  C12Q1/6874

Abstract: The invention generally relates to performing sandwich assays in droplets. In certain embodiments, the invention provides methods for detecting a target analyte that involve forming a compartmentalized portion of fluid including a portion of a sample suspected of containing a target analyte and a sample identifier, a first binding agent having a target identifier, and a second binding agent specific to the target analyte under conditions that produce a complex of the first and second binding agents with the target analyte, separating the complexes, and detecting the complexes, thereby detecting the target analyte.

公开(公告)号：US20210002703A1

公开(公告)日：2021-01-07

申请号：US17020041

申请日：2020-09-14

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Darren Roy Link ,  Benjamin J. Miller

IPC: C12Q1/686

Abstract: The invention generally relates to detecting target molecules.

公开(公告)号：US10808279B2

公开(公告)日：2020-10-20

申请号：US16460247

申请日：2019-07-02

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Darren Roy Link ,  Benjamin J. Miller

IPC: C12Q1/68 ,  C12Q1/686

Abstract: The invention generally relates to detecting target molecules.

公开(公告)号：US20200249230A1

公开(公告)日：2020-08-06

申请号：US16780508

申请日：2020-02-03

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Darren Roy Link ,  Michael L. Samuels

IPC: G01N33/573 ,  G01N33/58 ,  G01N33/542 ,  C12Q1/6827 ,  C12Q1/6818 ,  C12Q1/6804 ,  C12Q1/25 ,  G01N33/50 ,  C40B60/10 ,  C40B50/08 ,  C40B40/04 ,  B01F3/08 ,  B01L3/00 ,  B01F13/00 ,  B01J19/00 ,  B01F5/06

Abstract: The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the sunset. The amount of target can be determined based on the detected number.