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公开(公告)号：US20120225798A1
公开(公告)日：2012-09-06
申请号：US13369000
申请日：2012-02-08
Applicant: Charles R. Cantor , Chunming Ding , Yuk Ming Dennis Lo , Rossa Wai Kwum Chiu
Inventor： Charles R. Cantor , Chunming Ding , Yuk Ming Dennis Lo , Rossa Wai Kwum Chiu
IPC: C40B30/10
CPC classification number: C12Q1/6881 , C12Q1/6872 , C12Q2600/156 , C12Q2600/172 , C12Q2535/125
Abstract: The present invention is directed to methods of detecting nucleic acids in a biological sample. The method is based on a novel combination of a base extension reaction, which provides excellent analytical specificity, and a mass spectrometric analysis, which provides excellent specificity. The method can be used, for example, for diagnostic, prognostic and treatment purposes. The method allows accurate detection of nucleic acids that are present in very small amounts in a biological sample. For example, the method of the present invention is preferably used to detect fetal nucleic acid in a maternal blood sample; circulating tumor-specific nucleic acids in a blood, urine or stool sample; and donor-specific nucleic acids in transplant recipients. In another embodiment, one can detect viral, bacterial, fungal, or other foreign nucleic acids in a biological sample.
Abstract translation: 本发明涉及检测生物样品中核酸的方法。 该方法基于提供优异分析特异性的碱基延伸反应和提供优异特异性的质谱分析的新型组合。 该方法可用于例如诊断，预后和治疗目的。 该方法允许精确检测在生物样品中以非常少的量存在的核酸。 例如，本发明的方法优选用于检测母体血液样品中的胎儿核酸; 在血液，尿液或粪便样品中循环肿瘤特异性核酸; 和供体特异性核酸。 在另一个实施方案中，可以检测生物样品中的病毒，细菌，真菌或其它外来核酸。
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公开(公告)号：US07999095B2
公开(公告)日：2011-08-16
申请号：US12638871
申请日：2009-12-15
Applicant: Charles R. Cantor , Natalia E. Broude , Carlos Witte-Hoffman
Inventor： Charles R. Cantor , Natalia E. Broude , Carlos Witte-Hoffman
CPC classification number: C12Q1/6818 , C12Q1/6813 , C12Q2563/131 , C12Q2561/107
Abstract: The present invention is directed to novel methods for in vitro and in vivo detection of target nucleic acid molecules, including DNA and RNA targets, as well as nucleic acid analogues. The present invention is based on protein complementation, in which two individual polypeptides are inactive. When the two inactive polypeptide fragment are brought in close proximity during hybridization to a target nucleic acid, they re-associate into an active, detectable protein.
Abstract translation: 本发明涉及用于靶核酸分子（包括DNA和RNA靶标）以及核酸类似物的体外和体内检测的新方法。 本发明基于蛋白质互补，其中两个单独的多肽是无活性的。 当两个无活性多肽片段在与靶核酸杂交期间紧密接近时，它们重新连接成活性的可检测蛋白质。
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公开(公告)号：US20100297629A1
公开(公告)日：2010-11-25
申请号：US12638871
申请日：2009-12-15
Applicant: Charles R. Cantor , Natalia E. Broude , Carlos Witte-Hoffman
Inventor： Charles R. Cantor , Natalia E. Broude , Carlos Witte-Hoffman
CPC classification number: C12Q1/6818 , C12Q1/6813 , C12Q2563/131 , C12Q2561/107
Abstract: The present invention is directed to novel methods for in vitro and in vivo detection of target nucleic acid molecules, including DNA and RNA targets, as well as nucleic acid analogues. The present invention is based on protein complementation, in which two individual polypeptides are inactive. When the two inactive polypeptide fragment are brought in close proximity during hybridization to a target nucleic acid, they re-associate into an active, detectable protein.
Abstract translation: 本发明涉及用于靶核酸分子（包括DNA和RNA靶标）以及核酸类似物的体外和体内检测的新方法。 本发明基于蛋白质互补，其中两个单独的多肽是无活性的。 当两个无活性多肽片段在与靶核酸杂交期间紧密接近时，它们重新连接成活性的可检测蛋白质。
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公开(公告)号：US07709262B2
公开(公告)日：2010-05-04
申请号：US10589709
申请日：2005-02-18
Applicant: Charles R. Cantor , Chunming Ding
Inventor： Charles R. Cantor , Chunming Ding
CPC classification number: C12Q1/6827 , C12Q2600/156 , C12Q2525/186 , C12Q2535/125 , C12Q2565/627 , C12Q2545/101
Abstract: The present invention is directed to a method for detecting and quantifying rare mutations in a nucleic acid sample. The nucleic acid molecules under investigation can be either DNA or RNA. The rare mutation can be any type of functional or non-functional nucleic acid change or mutation, such as deletion, insertion, translocation, inversion, one or more base substitution or polymorphism. Therefore, the methods of the present invention are useful in detection of rare mutations in, for example, diagnostic, prognostic and follow-up applications, when the targets are rare known nucleic acid variants mixed in with the wildtype or the more common nucleic acid variant(s).
Abstract translation: 本发明涉及一种用于检测和定量核酸样品中罕见突变的方法。 正在研究的核酸分子可以是DNA或RNA。 罕见突变可以是任何类型的功能或非功能性核酸变化或突变，例如缺失，插入，易位，反转，一个或多个碱基取代或多态性。 因此，当目标是罕见的已知的与野生型或更常见的核酸变体混合的核酸变体时，本发明的方法可用于检测例如诊断，预后和随访应用中的罕见突变 （s）。
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公开(公告)号：US07662554B2
公开(公告)日：2010-02-16
申请号：US10529122
申请日：2003-10-09
Applicant: Charles R. Cantor , Natalia E. Broude , Carlos Witte-Hoffmann
Inventor： Charles R. Cantor , Natalia E. Broude , Carlos Witte-Hoffmann
CPC classification number: C12Q1/6818 , C12Q1/6813 , C12Q2563/131 , C12Q2561/107
Abstract: The present invention is directed to novel methods for in vitro and in vivo detection of target nucleic acid molecules, including DNA and RNA targets, as well as nucleic acid analogues. The present invention is based on protein complementation, in which two individual polypeptides are inactive. When the two inactive polypeptide fragment are brought in close proximity during hybridization to a target nucleic acid, they re-associate into an active, detectable protein.
Abstract translation: 本发明涉及用于靶核酸分子（包括DNA和RNA靶标）以及核酸类似物的体外和体内检测的新方法。 本发明基于蛋白质互补，其中两个单独的多肽是无活性的。 当两个无活性多肽片段在与靶核酸杂交期间紧密接近时，它们重新连接成活性的可检测蛋白质。
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Patent Agency Ranking
公开(公告)号：US20080248968A1
公开(公告)日：2008-10-09
申请号：US12123378
申请日：2008-05-19
Applicant: Daniel P. Little , Maryanne J. O'Donnell-Maloney , Charles R. Cantor , Hubert Koster
Inventor： Daniel P. Little , Maryanne J. O'Donnell-Maloney , Charles R. Cantor , Hubert Koster
IPC: C40B40/04
CPC classification number: H01J49/0418 , B01J19/0046 , B01J2219/00315 , B01J2219/00317 , B01J2219/00378 , B01J2219/00387 , B01J2219/00468 , B01J2219/00497 , B01J2219/005 , B01J2219/00504 , B01J2219/00511 , B01J2219/0052 , B01J2219/00527 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00637 , B01J2219/00641 , B01J2219/00648 , B01J2219/00659 , B01J2219/0072 , B01J2219/00722 , B01J2219/00725 , B01L3/0244 , B01L3/0262 , B01L3/0268 , C07B2200/11 , C07F9/2408 , C07F9/2429 , C07H21/00 , C40B40/06 , C40B40/10 , C40B60/14 , G01N35/1067 , G01N35/1074 , G01N2035/1037 , G01N2035/1069 , Y10T428/26 , Y10T428/31678 , Y10T428/31855 , Y10T436/119163 , Y10T436/24 , Y10T436/25 , Y10T436/2575
Abstract: Provided herein are substrates for matrix-assisted laser-desorption ionization (MALDI) mass spectrometric analysis. Each spot includes 3-hydroxypicolinic acid matrix and no analyte.
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公开(公告)号：US07179618B2
公开(公告)日：2007-02-20
申请号：US10285876
申请日：2002-11-01
Applicant: Takeshi Sano , Alexander N. Glazer , Charles R. Cantor
Inventor： Takeshi Sano , Alexander N. Glazer , Charles R. Cantor
CPC classification number: C07H21/04 , C07K14/36 , C07K14/825 , C07K2319/00 , C07K2319/22
Abstract: Streptavidin-metallothionein chimeric proteins with biological recognition specificity in which the streptavidin moiety provides high affinity biotin binding and the metallothionein moiety provides a high affinity metal binding. The binding affinity of the streptavidin-metallothionein chimeric protein both for biotin and heavy metal ions allows specific incorporation into, conjugation with, or labelling of any biological material containing biotin with various heavy metal ions.
Abstract translation: 具有生物识别特异性的链霉亲和素 - 金属硫蛋白嵌合蛋白，其中链霉亲和素部分提供高亲和力生物素结合和金属硫蛋白部分提供高亲和力的金属结合。 链霉抗生物素蛋白 - 金属硫蛋白嵌合蛋白对生物素和重金属离子的结合亲和力允许特异性掺入，缀合或标记含有生物素与各种重金属离子的任何生物材料。
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28.发明授权
公开(公告)号：US6124129A
公开(公告)日：2000-09-26
申请号：US19966
申请日：1998-02-06
Applicant: Przemyslaw Szafranski , Charlene Mello , Takeshi Sano , Cassandra L. Smith , David L. Kaplan , Charles R. Cantor
Inventor： Przemyslaw Szafranski , Charlene Mello , Takeshi Sano , Cassandra L. Smith , David L. Kaplan , Charles R. Cantor
Abstract: Compositions and methods for the control of genetically engineered organisms are described. A more effective cell suicide approach is contemplated based on the conditional expression of the lethal Streptomyces avidinii streptavidin gene. Toxicity of streptavidin is derived from its exceptionally high binding affinity for an essential prosthetic group, D-biotin. The general requirement for biotin through the living world makes streptavidin-based conditional lethal designs applicable to a broad range of containment strategies.
Abstract translation: 描述了用于控制转基因生物的组合物和方法。 基于致死性链霉菌链霉亲和素链霉亲和素基因的条件表达考虑了更有效的细胞自杀方法。 链霉抗生物素蛋白的毒性源自其对必需的假基团D-生物素的极高的结合亲和力。 生物素通过生活世界的一般要求使基于链霉亲和素的条件致命设计适用于广泛的遏制策略。
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公开(公告)号：US5726014A
公开(公告)日：1998-03-10
申请号：US123936
申请日：1993-09-17
Applicant: Cynthia A. Edwards , Charles R. Cantor , Beth M. Andrews , Lisa M. Turin
Inventor： Cynthia A. Edwards , Charles R. Cantor , Beth M. Andrews , Lisa M. Turin
IPC: C12N15/09 , C07K14/035 , C07K14/47 , C12N15/10 , C12N15/67 , C12Q1/68 , C12Q1/70 , C12P19/34 , G01N33/566
CPC classification number: C12Q1/6811 , C07K14/47 , C12N15/1048 , C12N15/67 , C12Q1/6883 , C12Q1/705
Abstract: The present invention defines a DNA:protein-binding assay useful for screening libraries of synthetic or biological compounds for their ability to bind DNA test sequences. The assay is versatile in that any number of test sequences can be tested by placing the test sequence adjacent to a defined protein binding screening sequence. Binding of molecules to these test sequence changes the binding characteristics of the protein molecule to its cognate binding sequence. When such a molecule binds the test sequence the equilibrium of the DNA:protein complexes is disturbed, generating changes in the concentration of free DNA probe. Numerous exemplary target test sequences (SEQ ID NO:1 to SEQ ID NO:600) are set forth. The assay of the present invention is also useful to characterize the preferred binding sequences of any selected DNA-binding molecule.
Abstract translation: 本发明定义了一种DNA：蛋白结合测定法，用于筛选合成或生物化合物文库结合DNA测试序列的能力。 该测定法是通用的，因为可以通过将测试序列置于与定义的蛋白质结合筛选序列相邻的位置来测试任何数量的测试序列。 分子与这些测试序列的结合改变了蛋白质分子与其同源结合序列的结合特征。 当这样的分子结合测试序列时，DNA：蛋白复合物的平衡受到干扰，产生游离DNA探针浓度的变化。 阐述了许多示例性目标测试序列（SEQ ID NO：1至SEQ ID NO：600）。 本发明的测定也可用于表征任何所选DNA结合分子的优选结合序列。
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30.发明授权DNA encoding streptavidin, streptavidin produced therefrom, fused polypeptides which include amino acid sequences present in streptavidin and uses thereof 失效编码链霉亲和素的DNA，由此产生的链霉抗生物素蛋白，包括存在于链霉亲和素中的氨基酸序列的融合多肽及其用途
公开(公告)号：US4839293A
公开(公告)日：1989-06-13
申请号：US833324
申请日：1986-02-24
Applicant: Charles R. Cantor , Richard Axel , Carlos Argarana
Inventor： Charles R. Cantor , Richard Axel , Carlos Argarana
IPC: C12N15/09 , C07K14/36 , C07K14/705 , C12N1/20 , C12N5/10 , C12P21/02 , C12R1/19 , C12R1/465 , C12R1/91
CPC classification number: C07K14/36 , C07K14/705 , C07K2319/00 , C07K2319/22 , C07K2319/33 , Y10S930/30
Abstract: DNA which encodes the polypeptide streptavidin has been isolated as a fragment 2 kb in length derived from a restriction endonuclease digestion of the chromosomal DNA of Streptomyces avidinii. The nucleic acid sequence of the gene and the amino acid sequence of the polypeptide have been determined. A fused gene has been prepared which comprises the streptavidin gene fused to a gene encoding the human LDL receptor. Expression of the gene fusion results in a fused streptavidin-human LDL receptor polypeptide. Methods are provided for using the fused gene to produce labeled, chemically modified proteins in vivo and to isolate a protein knowing only the nucleotide sequence of the gene encoding the protein.
Abstract translation: 编码多肽链霉抗生物素蛋白的DNA已经被分离为长度为2kb的片段，其来源于阿维链霉菌的染色体DNA的限制性内切核酸酶消化。 已经确定了该基因的核酸序列和该多肽的氨基酸序列。 已经制备了融合基因，其包含与编码人LDL受体的基因融合的链霉亲和素基因。 基因融合的表达导致融合的链霉亲和素 - 人LDL受体多肽。 提供了使用融合基因在体内产生标记的，化学修饰的蛋白质并分离仅知道编码蛋白质的基因的核苷酸序列的蛋白质的方法。
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