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223 条记录 (耗时 0.053 秒)

    Determining non-nucleic acid molecule binding to target by competition with nucleic acid ligands
    21.
    发明申请
    Determining non-nucleic acid molecule binding to target by competition with nucleic acid ligands 有权
    通过与核酸配体竞争来测定与核酸分子结合的非核酸分子

    公开(公告)号：US20050048521A1

    公开(公告)日：2005-03-03

    申请号：US10746565

    申请日：2003-12-24

    申请人： Nebojsa Janjic ,  Larry Gold

    发明人： Nebojsa Janjic ,  Larry Gold

    IPC分类号： G01N33/50 ,  C12Q1/68 ,  G01N33/15 ,  G01N33/53 ,  G01N33/566 ,  G01N37/00 ,  G01N33/543

    CPC分类号： G01N33/53 ,  C12Q1/6811 ,  Y10S530/812 ,  C12Q2545/107 ,  C12Q2565/518

    摘要： A competitive binding assay is used to determine whether a non-nucleic acid molecule from a library of non-nucleic acid molecules binds to a target. The non-nucleic acid molecule competes with a labeled nucleic acid ligand for binding to the target which may be immobilized. Detecting displacement of labeled nucleic acid ligand from a complex of the labeled nucleic acid ligand and the target determines binding of the non-nucleic acid molecule to the target. The nucleic acid ligand may be immobilized and contacted with a labeled target to form a complex. Adding a non-nucleic acid molecule to the complex displaces labeled target from the complex, and detecting displacement of the labeled target determines binding of the non-nucleic acid molecule to the target. Labeled nucleic acid ligand or labeled target displaced from or remaining in the complex can be detected for detecting displacement. Nucleic acid ligands that bind to the target are identified by the SELEX method. The assay provides a high throughput screening method for determining whether a non-nucleic acid molecule binds to a target.

    摘要翻译： 使用竞争性结合测定来确定非核酸分子的文库中的非核酸分子是否与靶标结合。 非核酸分子与标记的核酸配体竞争结合靶，其可以被固定化。 检测标记的核酸配体从标记的核酸配体和靶的复合物的置换确定非核酸分子与靶标的结合。 核酸配体可以被固定化并与标记的靶接触以形成复合物。 向复合物中加入非核酸分子从复合物置换标记的靶，并且检测标记的靶的置换确定非核酸分子与靶的结合。 可以检测标记的核酸配体或从复合物中残留的残留的标记的靶标，以检测位移。 通过SELEX法鉴定与靶标结合的核酸配体。 该测定提供了用于确定非核酸分子是否结合靶的高通量筛选方法。

    Modified SELEX processes without purified protein
    24.
    发明授权
    Modified SELEX processes without purified protein 有权
    没有纯化蛋白质的修饰SELEX过程

    公开(公告)号：US06730482B2

    公开(公告)日：2004-05-04

    申请号：US10096641

    申请日：2002-03-12

    申请人： Larry Gold ,  Dominic A. Zichi ,  Jonathan Drew Smith

    发明人： Larry Gold ,  Dominic A. Zichi ,  Jonathan Drew Smith

    IPC分类号： C12Q168

    CPC分类号： C12N15/1048 ,  C12Q1/6811 ,  C12Q2523/313

    摘要： This invention is directed towards a method for obtaining nucleic acid ligands against target proteins without directly purifying the target proteins. The method used in the invention is called SELEX, which is an acronym for Systematic Evolution of Ligands by EXponential enrichment. The nucleic acid ligands of the invention are useful as diagnostic and therapeutic agents for diseases in which the targets proteins play a causative role.

    摘要翻译： 本发明涉及一种在不直接纯化靶蛋白的情况下获得针对靶蛋白的核酸配体的方法。 本发明中使用的方法称为SELEX，它是通过扩充浓缩的配体的系统演化的缩写。 本发明的核酸配体可用作靶蛋白起作用的疾病的诊断和治疗剂。

    Method for the automated generation of nucleic acid ligands
    26.
    发明授权
    Method for the automated generation of nucleic acid ligands 失效
    自动生成核酸配体的方法

    公开(公告)号：US06716580B2

    公开(公告)日：2004-04-06

    申请号：US09815171

    申请日：2001-03-22

    申请人： Larry Gold ,  Dominic A. Zichi ,  Robert D. Jenison ,  Daniel J. Schneider

    发明人： Larry Gold ,  Dominic A. Zichi ,  Robert D. Jenison ,  Daniel J. Schneider

    IPC分类号： C12Q168

    CPC分类号： C12N15/1048

    摘要： The present invention includes a method and device for performing automated SELEX. The steps of the SELEX process are performed at one or more work stations on a work surface by a robotic manipulator controlled by a computer. The invention also includes methods and reagents to obviate the need for size-fractionation of amplified candidate nucleic acids before beginning the next round of the SELEX process.

    摘要翻译： 本发明包括用于执行自动化SELEX的方法和装置。 SELEX过程的步骤由工作表面上的一个或多个工作站由计算机控制的机器人操纵器执行。 本发明还包括在开始下一轮SELEX过程之前消除对扩增的候选核酸的大小分级的需要的方法和试剂。

    Bivalent binding molecules of 7 transmembrane G protein-coupled receptors
    27.
    发明授权
    Bivalent binding molecules of 7 transmembrane G protein-coupled receptors 失效
    7跨膜G蛋白偶联受体的二价结合分子

    公开(公告)号：US06682886B1

    公开(公告)日：2004-01-27

    申请号：US09118525

    申请日：1998-07-17

    申请人： Larry Gold

    发明人： Larry Gold

    IPC分类号： C12Q168

    CPC分类号： G01N33/74 ,  C12Q1/6811 ,  G01N2500/00 ,  C12Q2541/101

    摘要： Described herein are methods for identifying and preparing bivalent binding molecules to 7 transmembrane G protein-coupled receptors. The methods disclosed herein are based on the SELEX method for generating high affinity nucleic acid ligands. SELEX is an acronym for Systematic Evolution of Ligands by EXponential enrichment. The methods of this invention combine two or more binding domains to two or more different epitopes of the same 7 transmembrane G protein-coupled receptor. These bivalent binding molecules are useful as therapeutic and diagnostic agents.

    摘要翻译： 本文描述了用于鉴定和制备二价结合分子至7个跨膜G蛋白偶联受体的方法。 本文公开的方法基于用于产生高亲和力核酸配体的SELEX方法。 SELEX是指数浓缩的配体的系统演化的缩写。 本发明的方法将两个或多个结合结构域结合到相同的7跨膜G蛋白偶联受体的两个或多个不同的表位。 这些二价结合分子可用作治疗和诊断剂。

    Nucleic acid ligand diagnostic biochip
    29.
    发明授权
    Nucleic acid ligand diagnostic biochip 有权
    核酸配体诊断生物芯片

    公开(公告)号：US06458543B1

    公开(公告)日：2002-10-01

    申请号：US09723517

    申请日：2000-11-28

    申请人： Larry Gold ,  Dan Drolet ,  Dom Zichi ,  Sumedha Jayasena ,  Steve Creighton ,  Stanley Gill

    发明人： Larry Gold ,  Dan Drolet ,  Dom Zichi ,  Sumedha Jayasena ,  Steve Creighton ,  Stanley Gill

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6839 ,  C07B2200/11 ,  C12Q1/6834 ,  C12Q1/6837 ,  C40B40/00 ,  Y10S977/924 ,  C12Q2565/514 ,  C12Q2565/101 ,  C12Q2541/101 ,  C12Q2525/205

    摘要： A Nucleic acid ligand “Biochip” is disclosed, consisting of a solid support to which one or more specific Nucleic acid ligands is attached in a spatially defined manner. Each Nucleic acid ligand binds specifically and avidly to a particular Target molecule contained within a Test mixture, such as a Bodily fluid. The Target molecules include, but are not limited to, proteins (cellular, viral, bacterial, etc.) hormones, sugars, metabolic byproducts, cofactor, and intermediates, drugs, and toxins. In principle, the Biochip could be used to test any chemically complex mixture provided that Nucleic acid ligands to components suspected of being present in the mixture are attached to the Biochip. Thus, the Nucleic acid ligand Biochip will have a wider use in environmental testing, etc.

    摘要翻译： 披露了一种核酸配体“生物芯片”，由一种或多种特定的核酸配体以空间限定的方式连接的固体支持物组成。 每个核酸配体特异性地和特异性地结合到包含在测试混合物例如体液内的特定靶分子。 靶分子包括但不限于蛋白质（细胞，病毒，细菌等）激素，糖，代谢副产物，辅因子和中间体，药物和毒素。 原则上，生物芯片可以用于测试任何化学复合物混合物，只要核酸配体与怀疑存在于混合物中的组分连接在Biochip上即可。 因此，核酸配体生物芯片将在环境测试等方面有更广泛的应用。

    Vascular endothelial growth factor (VEGF) nucleic acid ligand complexes
    30.
    发明授权
    Vascular endothelial growth factor (VEGF) nucleic acid ligand complexes 有权
    血管内皮生长因子（VEGF）核酸配体复合物

    公开(公告)号：US06426335B1

    公开(公告)日：2002-07-30

    申请号：US09254968

    申请日：2000-03-13

    申请人： Nebojsa Janjic ,  Larry Gold ,  Paul Schmidt ,  Chandra Vargeese ,  Michael Willis

    发明人： Nebojsa Janjic ,  Larry Gold ,  Paul Schmidt ,  Chandra Vargeese ,  Michael Willis

    IPC分类号： A61K3170

    CPC分类号： A61K31/70 ,  A61K38/00 ,  A61K47/544 ,  A61K47/60 ,  C07H21/00 ,  C07K14/52 ,  C12N15/115 ,  C12N15/88 ,  C12N2310/3515

    摘要： This invention discloses a method for preparing a complex comprised of a VEGF Nucleic Acid Ligand and a Non-Immunogenic, High Molecular Weight Compound or Lipophilic Compound by identifying a VEGF Nucleic Acid Ligand by SELEX methodology and associating the VEGF Nucleic Acid Ligand with a Non-Immunogenic, High Molecular Weight Compound or Lipophilic Compound. The invention further discloses Complexes comprising one or more VEGF Nucleic Acid Ligands in association with a Non-Immunogenic, High Molecular Weight Compound or Lipophilic Compound. The invention further includes a Lipid construct comprising a VEGF Nucleic Acid Ligand or Complex and methods for making the same.

    摘要翻译： 本发明公开了一种通过SELEX方法鉴定VEGF核酸配体并将VEGF核酸配体与非 - 免疫原性，高分子量化合物或非亲和性化合物组合的复合物， 免疫原性，高分子量化合物或亲脂性化合物。 本发明进一步公开了包含与非免疫原性，高分子量化合物或亲脂性化合物结合的一种或多种VEGF核酸配体的复合物。 本发明还包括含有VEGF核酸配体或复合物的脂质构建体及其制备方法。