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14 条记录 (耗时 0.014 秒)

    Methods for the integrated synthesis and purification of oligonucleotides
    8.
    发明授权
    Methods for the integrated synthesis and purification of oligonucleotides 有权

    公开(公告)号：US07098326B2

    公开(公告)日：2006-08-29

    申请号：US10349195

    申请日：2003-01-22

    申请人： Wolfgang Pieken ,  Andreas Wolter ,  Michael Leuck

    发明人： Wolfgang Pieken ,  Andreas Wolter ,  Michael Leuck

    IPC分类号： C07H21/00 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C07H21/00 ,  C07H21/04

    摘要： The present invention discloses novel methods for the integrated synthesis and purification of oligonucleotides. The methods employ novel capping reagents carrying two functional groups. The first functional group provides for a smooth and efficient capping process and incorporates the second functional group into contaminant oligonucleotides during solid phase oligonucleotide synthesis. The second functional group functions as a chemical purification handle in the trapping of truncated oligonucleotides (failure sequences) on a solid support. The trapping process creates covalent bonds between the solid support and the truncated oligonucleotides and therefore allows the removal of the truncated sequences from the desired full length oligonucleotide product by filtration. The chemical trapping process employed in this invention is based on cycloaddition reactions, particularly Diels-Alder reactions between the truncated oligonucleotides and the trapping agent. The invention includes novel solid support compositions that carry covalently attached Diels-Alder reaction components.

    2'-deoxy-2'-fluoro modified RNA
    9.
    发明申请
    2'-deoxy-2'-fluoro modified RNA 审中-公开
    2'-脱氧-2'-氟修饰的RNA

    公开(公告)号：US20060036087A1

    公开(公告)日：2006-02-16

    申请号：US10656270

    申请日：2003-09-05

    申请人： Fritz Eckstein ,  Wolfgang Pieken ,  Fritz Benseler ,  David Olsen ,  David Williams ,  Olaf Heindenreich

    发明人： Fritz Eckstein ,  Wolfgang Pieken ,  Fritz Benseler ,  David Olsen ,  David Williams ,  Olaf Heindenreich

    IPC分类号： C07H21/02

    CPC分类号： C07H21/02 ,  B65G47/846

    摘要： The present invention refers to an RNA molecule with catalytic activity comprising at least one modified nucleoside, wherein the hydroxy group at the 2′-position of the ribose sugar is replaced by a modifier group, selected from halo, sulfhydryl, azido, amino, monosubstituted amino and disubstituted amino groups, a process for the preparation of modified RNA molecules and the use of modified RNA molecules as therapeutic agents and biocatalysts.

    摘要翻译： 本发明涉及具有至少一种修饰核苷的催化活性的RNA分子，其中核糖2'-位的羟基被选自卤素，巯基，叠氮基，氨基，单取代的修饰基团 氨基和二取代的氨基，制备修饰的RNA分子的方法和使用修饰的RNA分子作为治疗剂和生物催化剂。