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21.发明授权公开(公告)号：US08241842B2
公开(公告)日：2012-08-14
申请号：US11009901
申请日：2004-12-10
IPC分类号： C12Q1/00
CPC分类号： G01R33/56341
摘要： The present invention relates generally to the field of magnetic resonance imaging and, more particularly, to devices and methods used in the observation of the diffusion of molecules across a permeable membrane using magnetic resonance techniques. A typical embodiment of the invention is a method of observing the diffusion of a molecule in a container having a permeable membrane that is disposed between a first solution and a second solution in the container, by using magnetic resonance imaging to obtain an image of the diffusion of the molecule in the first solution across the membrane in to the second solution.
摘要翻译： 本发明一般涉及磁共振成像领域，更具体地，涉及用于使用磁共振技术观察分子穿过可渗透膜扩散的装置和方法。 本发明的典型实施例是通过使用磁共振成像来观察分子在具有设置在容器中的第一溶液和第二溶液之间的渗透膜的容器中的扩散的方法，以获得扩散图像 的第一溶液中的分子穿过膜进入第二溶液。
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公开(公告)号：US08124751B2
公开(公告)日：2012-02-28
申请号：US12790379
申请日：2010-05-28
申请人： Niles A. Pierce , Robert Dirks , Scott E. Fraser
发明人： Niles A. Pierce , Robert Dirks , Scott E. Fraser
CPC分类号： C12Q1/682 , C12Q1/6818 , C12Q1/6841 , Y10T436/143333 , C12Q2563/107 , C12Q2537/149 , C12Q2525/301
摘要： The present invention relates to the use of fluorescently labeled nucleic acid probes to identify and image analytes in a biological sample. In the preferred embodiments, a probe is provided that comprises a target region able to specifically bind an analyte of interest and an initiator region that is able to initiate polymerization of nucleic acid monomers. After contacting a sample with the probe, labeled monomers are provided that form a tethered polymer. Triggered probes and self-quenching monomers can be used to provide active background suppression.
摘要翻译： 本发明涉及使用荧光标记的核酸探针来鉴定和成像生物样品中的分析物。 在优选的实施方案中，提供了包含能够特异性结合目标分析物的靶区域和能够引发核酸单体聚合的引发区域的探针。 在将样品与探针接触后，提供形成系留聚合物的标记单体。 触发的探针和自熄单体可用于提供活性背景抑制。
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公开(公告)号：US20110134521A1
公开(公告)日：2011-06-09
申请号：US12916124
申请日：2010-10-29
申请人： Thai V. Truong , John M. Choi , Scott E. Fraser , Willy Supatto , David S. Koos
发明人： Thai V. Truong , John M. Choi , Scott E. Fraser , Willy Supatto , David S. Koos
IPC分类号： G02B21/06
CPC分类号： G02B21/16 , G01N21/6408 , G01N21/6428 , G01N21/6458 , G01N21/6486 , G01N2021/655 , G02B21/002 , G02B21/367
摘要： An apparatus for and method of performing light sheet microscopy (LISH) and light scanning microscopy (RAPS) in a single device are provided. The dual-mode imaging microscope allows for the use of both LISH and RAPS in a single instrument. This dual-mode device will allow researchers to have access to both types of microscopy, allowing access to the widest possible selection of samples. In addition, the device will reduce the high costs and space requirements associated with owning two different microscopes (LISH and RAPS).
摘要翻译： 提供了在单个装置中执行光谱显微镜（LISH）和光扫描显微镜（RAPS）的装置和方法。 双模式成像显微镜允许在单个仪器中使用LISH和RAPS。 这种双模式设备将使研究人员能够访问这两种类型的显微镜，从而允许访问尽可能多的样品。 此外，该设备将减少与拥有两台不同显微镜（LISH和RAPS）相关的高成本和空间要求。
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24.发明申请METHOD APPLYING HEMODYNAMIC FORCING AND KLF2 TO INITIATE THE GROWTH AND DEVELOPMENT OF CARDIAC VALVES 审中-公开应用血液动力学和KLF2方法来启动心脏瓣膜的生长和发育公开(公告)号：US20110033933A1
公开(公告)日：2011-02-10
申请号：US12837369
申请日：2010-07-15
CPC分类号： A61L27/3625 , A61F2/2415 , A61L27/3834 , A61L27/507 , A61L2430/20 , C12M21/08 , C12M25/14 , C12M29/12 , C12M35/04 , C12N5/0657 , C12N2501/15 , C12N2501/165 , C12N2521/00
摘要： A method for forming a cardiovascular structure in culture is provided. The method includes applying mechanical force to a cell population in culture such that a cardiovascular structure is formed. In some embodiments, the mechanical force is produced in culture medium by a pulsatile liquid flow with a retrograde component. The cell population can include stem cells or differentiated cells, or combinations of both. In particular embodiments, a cardiovascular valve is formed. Scaffolds for the support and growth of the cell population, and bioreactors including the scaffolds, are also provided.
摘要翻译： 提供了一种在培养中形成心血管结构的方法。 该方法包括对培养物中的细胞群体施加机械力，从而形成心血管结构。 在一些实施方案中，通过具有逆行组分的脉动液体流在培养基中产生机械力。 细胞群可包括干细胞或分化细胞，或两者的组合。 在特定实施例中，形成心血管瓣膜。 还提供了用于支持和生长细胞群体的支架，以及包括支架的生物反应器。
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25.发明授权Biological and chemical microcavity resonant sensors and methods of detecting molecules 有权生物和化学微腔谐振传感器和分子检测方法公开(公告)号：US07781217B2
公开(公告)日：2010-08-24
申请号：US11733480
申请日：2007-04-10
IPC分类号： G01N23/00
CPC分类号： H01S3/0627 , G01N21/7746 , G02B6/12007 , G02B6/29341 , G02B6/29343 , H01S3/0604 , H01S3/083 , H01S3/094053 , H01S3/1608 , H01S3/17
摘要： Resonant sensors and methods of detecting specific molecules with enhanced sensitivity. Optical energy is introduced into a microcavity, such as a silica toroid-shaped microcavity. The microcavity sensor has a functionalized outer surface and a sufficiently high Q value to generate an evanescent optical field with increased intensity. A molecule bound to the functionalized outer surface interacts with the external optical field, thereby heating the microcavity and generating a detectable resonant wavelength shift, which indicates a small number of molecules, even a single molecule, without the use of fluorescent or metal labels. Resonant sensors and methods can also be used to detect specific molecules, even a single molecule, within an environment. One application is detecting very small quantities or a single molecule of heavy water in ordinary water.
摘要翻译： 共振传感器和检测具有增强灵敏度的特定分子的方法。 将光能引入到微腔中，例如二氧化硅环形微腔。 微腔传感器具有功能化的外表面和足够高的Q值以产生具有增加的强度的渐逝光场。 结合到官能化外表面的分子与外部光场相互作用，从而加热微腔并产生可检测的共振波长漂移，其表示少量分子，甚至单个分子，而不使用荧光或金属标记。 谐振传感器和方法也可用于检测环境中的特定分子，甚至单个分子。 一种应用是在普通水中检测非常少的量或单一分子的重水。
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公开(公告)号：US07727544B2
公开(公告)日：2010-06-01
申请号：US11124673
申请日：2005-05-09
申请人： Daniel M. Schwartz , Chang Jun Yu , Robert H. Grubbs , Julia A. Kornfield , Scott E. Fraser , Matthew S. Mattson
发明人： Daniel M. Schwartz , Chang Jun Yu , Robert H. Grubbs , Julia A. Kornfield , Scott E. Fraser , Matthew S. Mattson
CPC分类号： A61K31/135 , A61K31/295 , A61K31/325 , A61K31/37 , A61K31/382 , A61K31/47 , A61K31/495 , A61K41/0042 , A61K49/0002
摘要： The present invention relates to altering the physical and/or chemical properties of at least part of at least one tissue in the eye. In a specific embodiment, it relates to the treatment and/or prevention of myopia. An activating energy source is utilized to photopolymerize or crosslink molecules in the sclera, thereby increasing the strength of the tissue. The individual is administered a crosslinking reagent or photopolymerizable molecule that becomes associated with the membrane, which is then precisely exposed to an energy source, such as light or ultrasound.
摘要翻译： 本发明涉及改变眼睛中至少一个组织的至少一部分的物理和/或化学性质。 在具体实施方案中，其涉及治疗和/或预防近视。 活化能源用于光聚合或交联巩膜中的分子，从而增加组织的强度。 给个体施用交联剂或可光聚合的分子，其与膜相关联，然后将其精确地暴露于能量源，例如光或超声波。
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公开(公告)号：US20090054267A1
公开(公告)日：2009-02-26
申请号：US12216062
申请日：2008-06-27
IPC分类号： C40B60/12
CPC分类号： G01N29/036 , B82Y5/00 , B82Y35/00 , G01N33/54373 , G01N2291/0257
摘要： A biofunctionalized nanoelectromechanical device (BioNEMS) for sensing single-molecules in solution by measuring the variation in the mechanical displacement of the BioNEMS device during a binding event is provided. The biofunctionalized nanoelectromechanical device according to the invention generally comprises a nanomechanical mechanical resonator, a detector integral with the mechanical resonator for measuring the mechanical displacement of the resonator, and electronics connected to the detector for communicating the results to a user. A system of biofunctionalized nanoelectromechanical devices and a method for utilizing the biofunctionalized nanoelectromechanical device of the present invention are also provided.
摘要翻译： 提供了一种生物功能化的纳米机电装置（BioNEMS），用于通过在绑定事件期间测量BioNEMS装置的机械位移的变化来感测溶液中的单分子。 根据本发明的生物功能化纳米机电装置通常包括纳米机械机械谐振器，与用于测量谐振器的机械位移的机械谐振器集成的检测器，以及连接到检测器的电子装置，用于将结果传达给用户。 还提供了一种生物功能化的纳米机电装置系统和利用本发明的生物功能化纳米机电装置的方法。
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公开(公告)号：US06180352B2
公开(公告)日：2001-01-30
申请号：US09459191
申请日：1999-12-10
IPC分类号： C12Q168
CPC分类号： C07H21/00 , C07H23/00 , C12Q1/6818 , C12Q1/6825 , Y02A50/52 , C12Q2565/607 , C12Q2565/401 , C12Q2563/137 , C12Q2563/113 , C12Q2565/101 , C12Q2565/1015
摘要： The present invention provides for the selective covalent modification of nucleic acids with redox active moieties such as transition metal complexes. Electron donor and electron acceptor moieties are covalently bound to the ribose-phosphate backbone of a nucleic acid at predetermined positions. The resulting complexes represent a series of new derivatives that are bimolecular templates capable of transferring electrons over very large distances at extremely fast rates. These complexes possess unique structural features which enable the use of an entirely new class of bioconductors and photoactive probes.
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公开(公告)号：US08593638B2
公开(公告)日：2013-11-26
申请号：US12573009
申请日：2009-10-02
申请人： Tao Lu , Tsu-Te Judith Su , Kerry J. Vahala , Scott E. Fraser
发明人： Tao Lu , Tsu-Te Judith Su , Kerry J. Vahala , Scott E. Fraser
IPC分类号： G01B9/02
CPC分类号： G01N21/7746
摘要： Resonant sensors and molecule detection methods utilizing split frequency. Optical energy is introduced into a microcavity, such as a toroid-shaped or spherical microcavity. A portion of the optical energy is backscattered and interacts with the introduced optical energy to form first and second modes of optical energy at respective first and second frequencies, also referred to as split frequency or mode doublets. One or more molecules bind to an outer surface of the microcavity and interact with an evanescent field of optical energy resonating within the microcavity. Binding of one or more molecules to the outer surface is detected based at least in part upon a change of the split frequency relative to a baseline split frequency.
摘要翻译： 谐振传感器和分子检测方法利用分频。 将光能引入微腔中，例如环形或球形微腔。 光能的一部分被反向散射并与引入的光能相互作用以在相应的第一和第二频率处形成第一和第二光能模式，也被称为分裂频率或模式双峰。 一个或多个分子结合到微腔的外表面并与在微腔内共振的光能的消逝场相互作用。 至少部分地基于分割频率相对于基线分割频率的变化来检测一个或多个分子与外表面的结合。
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30.发明授权Dynamic motion contrast and transverse flow estimation using optical coherence tomography 有权使用光学相干断层扫描的动态运动对比度和横向流量估计公开(公告)号：US08369594B2
公开(公告)日：2013-02-05
申请号：US13179377
申请日：2011-07-08
CPC分类号： A61B5/0261 , A61B3/102 , A61B5/0066 , G01N21/4795 , G01N2021/1787
摘要： The methods described herein are methods to ascertain motion contrast within optical coherence tomography data based upon phase variance. The phase variance contrast observes the nanometer scale motion of scatterers associated with Brownian motion and other non-flow motion. The inventive method of calculating motion contrast from the phase variance can differentiate regions of different mobility based on the motion contrast differences, and can use the phase information to characterize mobility properties of the scatterers. In flow regions, the inventive method for acquiring and analyzing motion contrast can identify the regions as well as characterize the motion. Furthermore, the inventive method can determine quantitative flow estimation, the index of refraction variations, and absorption variations within flow regions.
摘要翻译： 本文描述的方法是基于相位方差来确定光学相干断层摄影数据内的运动对比度的方法。 相位差对比度观察与布朗运动和其他非流动运动相关的散射体的纳米级运动。 从相位方差计算运动对比度的本发明方法可以基于运动对比差异来区分不同移动性的区域，并且可以使用相位信息来表征散射体的移动性质。 在流动区域中，用于获取和分析运动对比度的本发明的方法可以识别区域以及表征运动。 此外，本发明的方法可以确定流量区域中的定量流量估计，折射率变化指数和吸收变化。
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