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公开(公告)号：US20070243613A1
公开(公告)日：2007-10-18
申请号：US11709119
申请日：2007-02-20
CPC分类号： B01L3/0268 , B01L3/5085 , B01L2200/12 , B01L2300/069 , B01L2300/10 , B01L2300/1888 , B01L2400/0439 , C12M23/20 , C12M25/06 , C12N5/0068 , C12N2533/12 , C12N2535/10
摘要： A main object of the invention is to provide a new method for producing a cell culture substrate used to cause cells to adhere in a highly precise form onto a base material and then culture the cells. To attain the object, the invention provides a method for producing a cell culture substrate comprising: a patterning substrate forming process of forming, on a base material, a cell culture patterning layer wherein a cell adhesion portion having cell adhesive properties and a cell adhesion-inhibiting portion having cell adhesion-inhibiting properties can be formed by action of a photocatalyst by energy irradiation, thereby forming a patterning substrate; an energy irradiating process of irradiating the energy onto the cell culture patterning layer, thereby forming the cell adhesion portion and the cell adhesion-inhibiting portion in a pattern form by action of the photocatalyst; and a cell-containing liquid applying process of applying a cell-containing liquid onto the cell adhesion portion by a region-selecting applying method of applying the cell-containing liquid selectively onto the patterned cell adhesion portion.
摘要翻译： 本发明的主要目的是提供一种用于生产细胞培养基质的新方法，该方法用于使细胞以高度精确的形式粘附到基材上，然后培养细胞。 为了达到上述目的，本发明提供一种细胞培养基材的制造方法，其特征在于，包括：在基材上形成细胞培养图案形成层的图案形成基板的形成工序，其中，具有细胞粘合性的细胞粘附部， 通过能量照射可以通过光催化剂的作用形成具有细胞粘附抑制特性的抑制部分，从而形成图案化衬底; 将能量照射到细胞培养图案化层上的能量照射方法，由此通过光催化剂的作用形成图案形式的细胞粘附部分和细胞粘附抑制部分; 以及通过选择性地将细胞含有液体施加到图案化细胞粘附部分上的区域选择施加方法将含细胞的液体施加到细胞粘附部分上的含细胞的液体施加方法。
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公开(公告)号：US08497117B2
公开(公告)日：2013-07-30
申请号：US13032998
申请日：2011-02-23
CPC分类号： B01L3/0268 , B01L3/5085 , B01L2200/12 , B01L2300/069 , B01L2300/10 , B01L2300/1888 , B01L2400/0439 , C12M23/20 , C12M25/06 , C12N5/0068 , C12N2533/12 , C12N2535/10
摘要： A main object of the invention is to provide a new method for producing a cell culture substrate used to cause cells to adhere in a highly precise form onto a base material and then culture the cells.To attain the object, the invention provides a method for producing a cell culture substrate comprising: a patterning substrate forming process of forming, on a base material, a cell culture patterning layer wherein a cell adhesion portion having cell adhesive properties and a cell adhesion-inhibiting portion having cell adhesion-inhibiting properties can be formed by action of a photocatalyst by energy irradiation, thereby forming a patterning substrate; an energy irradiating process of irradiating the energy onto the cell culture patterning layer, thereby forming the cell adhesion portion and the cell adhesion-inhibiting portion in a pattern form by action of the photocatalyst; and a cell-containing liquid applying process of applying a cell-containing liquid onto the cell adhesion portion by a region-selecting applying method of applying the cell-containing liquid selectively onto the patterned cell adhesion portion.
摘要翻译： 本发明的主要目的是提供一种用于生产细胞培养基质的新方法，该方法用于使细胞以高度精确的形式粘附到基材上，然后培养细胞。 为了达到上述目的，本发明提供一种细胞培养基材的制造方法，其特征在于，包括：在基材上形成细胞培养图案形成层的图案形成基板的形成工序，其中，具有细胞粘合性的细胞粘附部， 通过能量照射可以通过光催化剂的作用形成具有细胞粘附抑制特性的抑制部分，从而形成图案化衬底; 将能量照射到细胞培养图案化层上的能量照射方法，由此通过光催化剂的作用形成图案形式的细胞粘附部分和细胞粘附抑制部分; 以及通过选择性地将细胞含有液体施加到图案化细胞粘附部分上的区域选择施加方法将含细胞的液体施加到细胞粘附部分的含细胞的液体施加方法。
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公开(公告)号：US07919305B2
公开(公告)日：2011-04-05
申请号：US11062674
申请日：2005-02-22
CPC分类号： B01L3/0268 , B01L3/5085 , B01L2200/12 , B01L2300/069 , B01L2300/10 , B01L2300/1888 , B01L2400/0439 , C12M23/20 , C12M25/06 , C12N5/0068 , C12N2533/12 , C12N2535/10
摘要： A main object of the invention is to provide a new method for producing a cell culture substrate used to cause cells to adhere in a highly precise form onto a base material and then culture the cells.To attain the object, the invention provides a method for producing a cell culture substrate comprising: a patterning substrate forming process of forming, on a base material, a cell culture patterning layer wherein a cell adhesion portion having cell adhesive properties and a cell adhesion-inhibiting portion having cell adhesion-inhibiting properties can be formed by action of a photocatalyst by energy irradiation, thereby forming a patterning substrate; an energy irradiating process of irradiating the energy onto the cell culture patterning layer, thereby forming the cell adhesion portion and the cell adhesion-inhibiting portion in a pattern form by action of the photocatalyst; and a cell-containing liquid applying process of applying a cell-containing liquid onto the cell adhesion portion by a region-selecting applying method of applying the cell-containing liquid selectively onto the patterned cell adhesion portion.
摘要翻译： 本发明的主要目的是提供一种用于生产细胞培养基质的新方法，该方法用于使细胞以高度精确的形式粘附到基材上，然后培养细胞。 为了达到上述目的，本发明提供一种细胞培养基材的制造方法，其特征在于，包括：在基材上形成细胞培养图案形成层的图案形成基板的形成工序，其中，具有细胞粘合性的细胞粘附部， 通过能量照射可以通过光催化剂的作用形成具有细胞粘附抑制特性的抑制部分，从而形成图案化衬底; 将能量照射到细胞培养图案化层上的能量照射方法，由此通过光催化剂的作用形成图案形式的细胞粘附部分和细胞粘附抑制部分; 以及通过选择性地将细胞含有液体施加到图案化细胞粘附部分上的区域选择施加方法将含细胞的液体施加到细胞粘附部分的含细胞的液体施加方法。
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24.发明申请Method for producing cell culture substrate and apparatus for producing cell culture substrate 失效细胞培养基质的制造方法和细胞培养基材的制造装置公开(公告)号：US20070141697A1
公开(公告)日：2007-06-21
申请号：US10594174
申请日：2005-03-24
CPC分类号： C12M25/06 , C12M33/00 , C12N5/0068 , C12N2535/10 , Y10S977/703
摘要： A method for producing a cell culture substrate to which cells adhere in a finely processed pattern while retaining the pattern for a long period of time to culture the cells and a production apparatus used in the production method. The method includes: forming a patterning substrate by forming: on a base material, a light shielding portion, and a cell adhesive layer having adhesion to a cell and containing a cell adhesive material which is decomposed or denatured by an action of a photocatalyst upon energy irradiation so as to cover the light shielding portion; an energy irradiating process of irradiating energy to the patterning substrate from the base material side to form a pattern; and a cell adhesion process of making the cell adhere to the cell adhesion portion in a cell culture medium containing the cell and a culture medium.
摘要翻译： 细胞培养基板的制造方法，其中细胞以精细加工的图案粘附，同时长期保持图案以培养细胞，以及生产方法中使用的制造装置。 该方法包括：通过在基底材料，遮光部分和具有对细胞粘附的细胞粘合剂层上形成图案形成衬底，并且含有通过光催化剂的作用而被分解或变性的细胞粘附材料的能量 照射以覆盖遮光部分; 能量照射处理，其从所述基材侧向所述图案化衬底照射能量以形成图案; 以及在含有细胞和培养基的细胞培养基中使细胞粘附于细胞粘附部的细胞粘附过程。
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公开(公告)号：US08669098B2
公开(公告)日：2014-03-11
申请号：US12899007
申请日：2010-10-06
申请人： Hideshi Hattori
发明人： Hideshi Hattori
IPC分类号： C12M1/34
CPC分类号： B01J19/0046 , B01J2219/00527 , B01J2219/0054 , B01J2219/00596 , B01J2219/00605 , B01J2219/00612 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , C40B70/00
摘要： For the purpose of improving the accuracy of a variety analyses using a bio-microarray, it is a main object of the invention to provide a bio-microarray capable of producing relatively high signal intensity from a fluorescent molecule and capable of having improved quantification performance and to provide a substrate for bio-microarray.In order to achieve the object, the invention provides a substrate for bio-microarray characterized by having a reflection-suppressing function.
摘要翻译： 为了提高使用生物微阵列的多样性分析的准确性，本发明的主要目的是提供能够从荧光分子产生相对高的信号强度且能够提高定量性能的生物微阵列， 以提供用于生物微阵列的底物。 为了达到上述目的，本发明提供一种生物微阵列用基板，其特征在于具有反射抑制功能。
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公开(公告)号：US08153548B2
公开(公告)日：2012-04-10
申请号：US12662468
申请日：2010-04-19
CPC分类号： B01J23/002 , B01J21/066 , B01J23/58 , B01J23/6527 , B01J2523/00 , C10G35/085 , C10G45/62 , C10G2300/305 , C10G2300/70 , B01J2523/48 , B01J2523/69 , B01J2523/828 , B01J2523/12
摘要： The isomerization catalyst is a solid acid catalyst formed with a base of tungstated zirconium mixed oxides loaded with at least one hydrogenation/dehydrogenation metal catalyst from Groups 8-10 (IUPAC, 2006) and impregnated with at least one alkali metal from Group 1 (IUPAC, 2006). The metal from Groups 8-10 is preferably selected from platinum, palladium, ruthenium, rhodium, iridium, osmium and mixtures thereof, and most preferably is platinum. The Group I alkali metal is selected from lithium, sodium, potassium rubidium and cesium and mixtures thereof, and is preferably lithium, sodium, or potassium. Preferable, the catalyst forms, by weight, a base having between about 80-90% zirconium mixed oxides and between about 10-20% tungstate; between about 0.1-3.00% Group 8-10 metal; and between about 0.01-1.00% Group 1 alkali metal.
摘要翻译： 异构化催化剂是固体酸催化剂，其形成有装载有来自组8-10（IUPAC，2006）的至少一种氢化/脱氢金属催化剂的钨酸锆混合氧化物的基底，并用至少一种来自组1的碱金属（IUPAC） ，2006）。 来自组8-10的金属优选选自铂，钯，钌，铑，铱，锇及其混合物，最优选为铂。 I族碱金属选自锂，钠，钾铷和铯及其混合物，优选锂，钠或钾。 优选的是，催化剂重量含有约80-90％的锆混合氧化物和约10-20％的钨酸盐之间的碱; 介于约0.1-3.00％之间的第8-10族金属; 和约0.01-1.00％的第1族碱金属。
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公开(公告)号：US20070015277A1
公开(公告)日：2007-01-18
申请号：US11446913
申请日：2006-06-05
申请人： Hideshi Hattori , Masatoshi Kuroda
发明人： Hideshi Hattori , Masatoshi Kuroda
CPC分类号： C12N5/0068 , C12N2533/30
摘要： A main object of the present invention is to provide a cell transfer substrate capable of transplanting cells, maintaining a pattern as it is, on a living body tissue or the like, even when: the size of the cell sheet is extremely small; the cells are cultured sparsely; the cells are in a form of a small colony; or the cells are cultured in a pattern, for example, as a blood vessel, a vessel network such as a lymphatic vessel, or a nerve network, and to provide a substrate for cell transfer to be used for the cell transfer substrate. In order to achieve the above-mentioned object, the present invention provides a substrate for cell transfer comprising: a polymer base material; an intermediate layer formed on the polymer base material; and a cell transfer layer formed on the intermediate layer.
摘要翻译： 本发明的主要目的在于，提供一种能够将细胞移植到生物体组织等上的能够移动细胞的细胞转移基板，即使在细胞片的尺寸非常小的情况下， 细胞稀少培养; 细胞呈小菌落的形式; 或细胞以例如血管，血管网络例如淋巴管或神经网络的方式进行培养，并提供用于细胞转移基质的细胞转移用基板。 为了实现上述目的，本发明提供了一种用于电池转印的基板，包括：聚合物基材; 形成在聚合物基材上的中间层; 以及形成在中间层上的电池转印层。
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公开(公告)号：US06773801B2
公开(公告)日：2004-08-10
申请号：US10115790
申请日：2002-04-03
申请人： Hideshi Hattori
发明人： Hideshi Hattori
IPC分类号： B32B516
CPC分类号： B05D7/56 , B05D1/185 , C03C17/007 , C03C2217/42 , G02B1/00 , G02B1/11 , Y10T428/24802 , Y10T428/24893 , Y10T428/25 , Y10T428/251 , Y10T428/256 , Y10T428/257 , Y10T428/258 , Y10T428/259 , Y10T428/2998
摘要： The present invention discloses a fine particle layer laminated film configured in such a manner that a plurality of fine particle layers containing the fine particles, of which average particle diameters are in the range of 1 nm and 95 nm, are laminated; wherein the fine particles in the fine particle layer laminated film are accreted by polymer molecules; there are air spaces within a film of the fine particle layer laminated film; and a density of the fine particles in the fine particle layer laminated film is in the range of 40% and 80% in percentage by volume. According to the present invention, it is possible to obtain a laminated film sufficiently having a property of the fine particle, for example, a property such as a low index of refraction or the like. Therefore, the present invention has an effect such that the fine particle layer laminated film according to the present invention can be preferably used for an application such as an antirelection film or the like.
摘要翻译： 本发明公开了一种微粒层叠层膜，其中层叠有平均粒径为1nm〜95nm范围的包含微粒的多个微粒层， 其中微粒层层叠膜中的细颗粒由聚合物分子引起; 在微粒层层叠膜的膜内存在空气空间; 并且细颗粒层层叠膜中的细颗粒的密度在40体积％和80体积％的范围内。 根据本发明，可以获得充分具有微粒的性质的层叠膜，例如，诸如低折射率等的性质。 因此，本发明具有这样的效果，使得本发明的微粒层层叠膜可以优选用于抗反射膜等应用。
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公开(公告)号：US09249386B2
公开(公告)日：2016-02-02
申请号：US11446913
申请日：2006-06-05
申请人： Hideshi Hattori , Masatoshi Kuroda
发明人： Hideshi Hattori , Masatoshi Kuroda
IPC分类号： C12N5/00
CPC分类号： C12N5/0068 , C12N2533/30
摘要： A main object of the present invention is to provide a cell transfer substrate capable of transplanting cells, maintaining a pattern as it is, on a living body tissue or the like, even when: the size of the cell sheet is extremely small; the cells are cultured sparsely; the cells are in a form of a small colony; or the cells are cultured in a pattern, for example, as a blood vessel, a vessel network such as a lymphatic vessel, or a nerve network, and to provide a substrate for cell transfer to be used for the cell transfer substrate. In order to achieve the above-mentioned object, the present invention provides a substrate for cell transfer comprising: a polymer base material; an intermediate layer formed on the polymer base material; and a cell transfer layer formed on the intermediate layer.
摘要翻译： 本发明的主要目的在于，提供一种能够将细胞移植到生物体组织等上的能够移动细胞的细胞转移基板，即使在细胞片的尺寸非常小的情况下， 细胞稀少培养; 细胞呈小菌落的形式; 或细胞以例如血管，血管网络例如淋巴管或神经网络的方式进行培养，并提供用于细胞转移基质的细胞转移用基板。 为了实现上述目的，本发明提供了一种用于电池转印的基板，包括：聚合物基材; 形成在聚合物基材上的中间层; 以及形成在中间层上的电池转印层。
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30.发明授权Test kit comprising a culture instrument with a cell pattern and a gel suitable to embed cell pattern 有权测试试剂盒包括具有细胞图案和适于嵌入细胞图案的凝胶的培养仪器公开(公告)号：US08741645B2
公开(公告)日：2014-06-03
申请号：US13092552
申请日：2011-04-22
CPC分类号： G01N33/5008 , G01N33/5029 , G01N33/5032
摘要： The present invention provides a method for performing a biological test under conditions in which an artificially prepared cell pattern with initial position coordinates that can be determined is three-dimensionally cultured within a gelled matrix. The present invention relates to a biological test method that comprises testing a biological indicator with reference to at least one selected from the group consisting of cell proliferation, cell movement, and cell differentiation in a cell pattern substantially embedded in gel. The present invention also relates to a kit for the biological test method.
摘要翻译： 本发明提供一种用于在可以测定初始位置坐标的人造制备的细胞图案在凝胶化基质内三维培养的条件下进行生物测试的方法。 本发明涉及一种生物测试方法，其包括参考选自基本上嵌入凝胶中的细胞图案中的细胞增殖，细胞运动和细胞分化中的至少一种来测试生物学指示剂。 本发明还涉及用于生物测试方法的试剂盒。
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