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公开(公告)号：US4252769A
公开(公告)日：1981-02-24
申请号：US106828
申请日：1979-12-26
IPC分类号： B01J19/00 , C40B40/06 , C40B60/14 , G01N33/483 , G01N35/00 , G01N35/10 , G01N1/18 , G01N9/30
CPC分类号： G01N35/00 , B01J19/0046 , B01J2219/00421 , B01J2219/0049 , B01J2219/00695 , B01J2219/00722 , C40B40/06 , C40B60/14 , G01N35/1079
摘要： An improved apparatus for the performance of chemical processes having a reaction chamber with an open end, a reaction cell mounted within the chamber and having a substantially cylindrical interior wall in which is formed an annular groove, and means for spinning the cell about its axis to distribute a chemical compound on the interior wall as a thin film, which includes a closure member mounted for rotation about an axis displaced from the axis of the cell, and a fluid conduit extending through the closure member and terminating at an inner end within the cell for the withdrawal of fluid therefrom. The inner end of the conduit is displaced from the axis of rotation of the closure member and is positioned substantially opposite the annular groove such that it is actuable for adjustment between a first location displaced radially from the annular groove and a second location substantially within the groove by rotation of the closure member through a preselected angle.
摘要翻译： 一种用于执行具有开口端的反应室的化学方法的改进装置，安装在室内的反应池，并具有基本上圆柱形的内壁，其中形成有环形槽，以及用于将电池绕其轴线旋转的装置 在内壁上分布化学化合物作为薄膜，其包括安装成围绕从电池轴线移动的轴线旋转的封闭构件以及延伸穿过封闭构件并终止于电池内部的内端的流体导管 用于从中排出流体。 导管的内端从闭合构件的旋转轴线移位并且基本上与环形槽相对地定位，使得其可致动以在从环形槽径向偏移的第一位置和基本上位于凹槽内的第二位置之间进行调节 通过关闭构件的旋转预定角度。
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公开(公告)号：US07364855B2
公开(公告)日：2008-04-29
申请号：US11119985
申请日：2005-05-02
CPC分类号： C12Q1/68 , C12Q1/6827 , C12Q1/6862 , C12Q2537/164 , C12Q2521/501
摘要： Ligation-based methods and kits are disclosed for determining the degree of methylation of one or more target nucleotides. In certain embodiments, the methylation status of one or more target nucleotides is determined by generating misligation products. In certain embodiments, at least one target nucleotide is amplified prior to the ligation reaction. In certain embodiments, at least one ligation product, at least one ligation product surrogate, at least one misligation product, at least one misligation product surrogate, or combinations thereof are amplified. In certain embodiments, one or more ligation probes comprise at least one nucleotide analog, at least one Modification, at least one mismatched nucleotide, or combinations thereof.
摘要翻译： 公开了基于连接的方法和试剂盒以确定一个或多个靶核苷酸的甲基化程度。 在某些实施方案中，通过产生错误产物来确定一个或多个靶核苷酸的甲基化状态。 在某些实施方案中，在连接反应之前扩增至少一种靶核苷酸。 在某些实施方案中，扩增至少一种连接产物，至少一种连接产物替代物，至少一种错误产物，至少一种错误产物替代物或其组合。 在某些实施方案中，一个或多个连接探针包含至少一个核苷酸类似物，至少一个修饰，至少一个错配核苷酸或其组合。
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公开(公告)号：US5942609A
公开(公告)日：1999-08-24
申请号：US191390
申请日：1998-11-12
CPC分类号： C12P19/34 , C12Q1/6834
摘要： The present invention concerns methods of assembly of a polynucleotide on a solid-support by performing steps of annealing, ligation, and extension. The steps may be repeated in a cyclical manner to assemble immobilized double- or single-stranded polynucleotides with functional gene properties. The immobilized polynucleotides may be amplified by the polymerase chain reaction, and detected and quantitated by an exonuclease assay with a self-quenching, fluorescent probe. The polynucleotide may be cleaved from the solid-support by chemical or enzymatic cleavage.
摘要翻译： 本发明涉及通过退火，连接和延伸的步骤将多核苷酸装配在固体支持物上的方法。 可以以循环方式重复这些步骤以组装具有功能基因特性的固定的双链或单链多核苷酸。 固定化的多核苷酸可以通过聚合酶链式反应来扩增，并通过使用自猝灭荧光探针的外切核酸酶测定进行检测和定量。 多核苷酸可以通过化学或酶切割从固体支持物切割。
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24.发明授权Automated system for providing a sequence of chemicals to a reaction process 失效用于向反应过程提供一系列化学物质的自动化系统
公开(公告)号：US5273715A
公开(公告)日：1993-12-28
申请号：US973137
申请日：1992-11-06
申请人： John Bridgham , Timothy G. Geiser , Michael W. Hunkapiller , Stephen B. H. Kent , Mark P. Marplott , Paul O. Ramstad
发明人： John Bridgham , Timothy G. Geiser , Michael W. Hunkapiller , Stephen B. H. Kent , Mark P. Marplott , Paul O. Ramstad
CPC分类号： C07K1/045 , B01J19/0046 , B01J2219/00286 , B01J2219/00389 , B01J2219/00484 , B01J2219/00488 , B01J2219/0049 , B01J2219/00495 , B01J2219/005 , B01J2219/0059 , B01J2219/00596 , B01J2219/00689 , B01J2219/00695 , B01J2219/00725 , C40B40/10 , C40B60/14
摘要： An automated system for providing a preselected sequence of chemicals to a reaction. This apparatus includes a track on which a set of cartridges are placed in a preselected order corresponding to an order in which they are to be used in a reaction process. These cartridges are moved past a point at which these chemicals are extracted for use in the process. The chemicals are preferably in liquid form and are contained in containing having a top seal through which a needle can penetrate to extract chemicals for use in the process. These containers preferably contain the aliquot portion needed for the process, thereby providing a mechanism for providing accurate amounts of each chemical to the process.
摘要翻译： 一种用于向反应提供预选序列化学物质的自动化系统。 该装置包括一个轨道，在该轨道上以预选的顺序放置一组墨盒，该预定顺序与它们在反应过程中使用的顺序相对应。 这些墨盒被移动通过这些化学品提取的点，以用于该过程。 化学品优选为液体形式，并且包含含有顶部密封件，针可穿过该顶部密封件以提取用于该方法中的化学品。 这些容器优选地含有该方法所需的等分试样部分，从而提供了一种用于向该方法提供精确量的每种化学品的机理。
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公开(公告)号：US5186898A
公开(公告)日：1993-02-16
申请号：US328488
申请日：1989-03-24
申请人： John Bridgham , Timothy G. Geiser , Michael W. Hunkapiller , Stephen B. H. Kent , Mark P. Marriott , Paul O. Ramstad , Eric S. Nordman
发明人： John Bridgham , Timothy G. Geiser , Michael W. Hunkapiller , Stephen B. H. Kent , Mark P. Marriott , Paul O. Ramstad , Eric S. Nordman
CPC分类号： C07K1/045 , B01J19/0046 , B01J2219/00286 , B01J2219/00389 , B01J2219/00484 , B01J2219/00488 , B01J2219/0049 , B01J2219/00495 , B01J2219/005 , B01J2219/0059 , B01J2219/00596 , B01J2219/00689 , B01J2219/00695 , B01J2219/00725 , C40B40/10 , C40B60/14 , Y10S436/81
摘要： An apparatus is provided for automatically constructing a polypeptide of high purity, up to 50 amino acids in length, using only single couplings. The apparatus includes an activation system for receiving protected amino acids, one kind at a time, having a common vessel (an activator vessel) in which to activate each of the amino acids. Also included is a reaction vessel for containing a resin used in solid-phase peptide synthesis for attaching a peptide chain thereto. A transfer system is also provided, which operates under control of a computer, to transfer the activated species from the activation system to the reaction vessel and to transfer amino acids, reagents, gases, and solvents from one part of the apparatus to another. The activator system also includes a temperature controlled concentrator vessel in which an activator solvent is replaced by a coupling solvent to enhance the coupling of the activated species to the peptide chain in the reaction vessel. Also included in the synthesizer system is a vortexer for affecting total washing of materials in the reaction vessel and the reaction vessel itself, an automated peptide resin sampling system, and an autodelivery system for providing individual containers of amino acid to the synthesizer in the order desired in the peptide sequence. A liquid sensor system is also included to monitor transitions between gases and liquids in specific tubes in the synthesizer in order to provide input signals to the computer system for control purposes. The computer system software which controls the operation of the synthesizer is organized according to a series of menus which allows the user of the system to select individual cycles of operation for each vessel in the synthesizer. In addition, an algorithm has been developed which provides for optimum efficiency in tbe production of a peptide for any given selection of cycles.
摘要翻译： 提供了一种用于仅使用单个耦合自动构建高纯度多达50个氨基酸长度的多肽的装置。 该装置包括用于一次接收受保护的氨基酸的激活系统，其具有在其中激活每个氨基酸的共同血管（活化剂容器）。 还包括用于含有固相肽合成中用于连接肽链的树脂的反应容器。 还提供了在计算机控制下操作的转移系统，以将活化物质从活化系统转移到反应容器中，并将氨基酸，试剂，气体和溶剂从设备的一部分转移到另一部分。 活化剂体系还包括温度控制的浓缩容器，其中活化剂溶剂被偶联溶剂代替以增强活化物质与反应容器中的肽链的偶联。 还包括在合成器系统中的是用于影响反应容器和反应容器本身中的材料的全部洗涤的涡流器，自动化肽树脂取样系统和用于按合适的顺序向合成器提供单个氨基酸容器的自动输送系统 在肽序列中。 还包括液体传感器系统以监测合成器中特定管中的气体和液体之间的转换，以便为控制目的向计算机系统提供输入信号。 控制合成器操作的计算机系统软件根据一系列菜单进行组织，这些菜单允许系统的用户为合成器中的每个容器选择各个操作周期。 此外，已经开发了一种算法，其为任何给定的循环选择提供肽的生产的最佳效率。
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公开(公告)号：US4610847A
公开(公告)日：1986-09-09
申请号：US602699
申请日：1984-04-23
IPC分类号： B01J4/02 , B01J19/00 , C40B40/10 , C40B60/14 , G01N33/483 , G01N35/00 , G01N35/10 , B01J19/26 , G01N33/68
CPC分类号： G01N35/00 , B01J19/0046 , B01J4/02 , B01J2219/00283 , B01J2219/00286 , B01J2219/00398 , B01J2219/00418 , B01J2219/00423 , B01J2219/00495 , B01J2219/0059 , B01J2219/00596 , B01J2219/00686 , B01J2219/00689 , B01J2219/00695 , B01J2219/00725 , C40B40/10 , C40B60/14 , G01N35/1079
摘要： Conversion flask for use is an apparatus for the sequential performance of chemical processes on a sample of chemical material. The flask includes at least three capillary tubes and a large bore tube extending into the interior thereof for the introduction and withdrawal of various fluids. The capillary tubes are constructed to introduce a plurality of fine bubbles into a liquid within the flask to agitate the liquid and accelerate drying, to impinge chemicals on the walls of the flask, and to produce a spray onto the interior walls of the flask in proximity to its upper end to wash down the interior walls of the flask.
摘要翻译： 用于使用的转炉是用于在化学材料样品上顺序执行化学过程的装置。 烧瓶包括至少三个毛细管和延伸到其内部的用于引入和取出各种流体的大孔管。 毛细管被构造成将多个细小气泡引入烧瓶内的液体中以搅拌液体并加速干燥，将化学物质撞击在烧瓶的壁上，并在接近的温度下在烧瓶的内壁上产生喷雾 到其上端以冲洗烧瓶的内壁。
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公开(公告)号：US4603114A
公开(公告)日：1986-07-29
申请号：US602516
申请日：1984-04-20
IPC分类号： B01J4/02 , B01J19/00 , C40B40/10 , C40B60/14 , G01N33/483 , G01N33/68 , G01N35/00 , G01N35/10 , G01N1/18 , G01N33/48
CPC分类号： G01N33/6818 , B01J19/0046 , B01J4/02 , G01N35/00 , B01J2219/00283 , B01J2219/00286 , B01J2219/00398 , B01J2219/00418 , B01J2219/00423 , B01J2219/00495 , B01J2219/0059 , B01J2219/00596 , B01J2219/00686 , B01J2219/00689 , B01J2219/00695 , B01J2219/00725 , C40B40/10 , C40B60/14 , G01N35/1079 , Y10T436/25
摘要： An improved method for the sequential performance of chemical processes on a sample of chemical material wherein the sample is embedded in a solid matrix of fluid permeable material located within a reaction chamber and is sequentially subjected to a plurality of fluids passed through the chamber in a pressurized stream, causing chemical interaction between the sample and the fluids.
摘要翻译： 一种用于在化学材料样品上顺序执行化学过程的改进方法，其中样品被嵌入在位于反应室内的流体可渗透材料的固体基质中，并且依次经受多个流体通过加压室 流，导致样品和流体之间的化学相互作用。
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公开(公告)号：US4558845A
公开(公告)日：1985-12-17
申请号：US421445
申请日：1982-09-22
CPC分类号： F16K31/06 , B01J19/0046 , F16K7/14 , B01J2219/00396 , C40B60/14 , Y10T137/87684 , Y10T137/87861 , Y10T137/87885
摘要： Briefly, the present invention comprises the improvement in a valve arrangement for controlling the flow of a fluid including a valve block having at least one substantially flat valving site on a surface thereof, the valve block defining passages communicating with the valving site through openings, a resilient diaphragm covering the valving site and forming a fluid tight seal with the valve block along the periphery of the valving site and a closure element having a substantially flat closure surface, the closure element being actuable between an open condition wherein the closure surface is retracted a preselected distance from the valving site and diaphragm, and a closed condition wherein the closure surface forces the diaphragm against the valving site; whereby the diaphragm can be urged away from the valving site by fluid pressure in the passages and the closure element is in the open condition, establishing a valve chamber above the valving site for fluid flow between the passages, and if forced against the valving site when the closure element is in the closed condition, eliminating the valve chamber and expelling any fluid therein; the said improvement for which a patent is sought comprising a valve block having said passages therein which are free of intersections within the valve block.
摘要翻译： 简而言之，本发明包括改进用于控制流体流动的阀门装置，该流体包括在其表面上具有至少一个基本上平坦的阀门位置的阀块，阀块限定通过开口与阀门部分连通的通道， 覆盖阀门位置的弹性膜片，并且沿着阀门位置的周边与阀块形成流体密封的密封件，以及具有基本上平坦的封闭表面的封闭元件，封闭元件可在打开状态之间致动，其中封闭表面缩回 从阀门和隔膜预先选定的距离，以及闭合状态，其中闭合表面迫使隔膜抵靠阀门位置; 由此隔膜可以通过通道中的流体压力被迫离开阀门位置，并且闭合元件处于打开状态，在阀位之上建立阀室，用于在通道之间流体流动，并且如果在阀门位置被强制抵靠阀门位置时， 闭合元件处于关闭状态，消除阀室并排出其中的任何流体; 所寻求的专利的所述改进包括具有其中具有在阀块内没有交叉的所述通道的阀块。
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公开(公告)号：USRE43096E1
公开(公告)日：2012-01-10
申请号：US10389663
申请日：2003-03-13
申请人： Lloyd M. Smith , Leroy E. Hood , Michael W. Hunkapiller , Timothy Hunkapiller , Charles R. Connell
发明人： Lloyd M. Smith , Leroy E. Hood , Michael W. Hunkapiller , Timothy Hunkapiller , Charles R. Connell
IPC分类号： C12Q1/68 , G01N27/447
CPC分类号： G01N27/44726 , C12Q1/6816 , C12Q1/6869 , G01N27/44721 , C12Q2563/107 , C12Q2535/101
摘要： This invention provides a duplex comprising an oligonucleotide primer and a template, wherein the primer is coupled chemically to a chromophore or fluorophore so as to allow chain extension by a polymerase. In one embodiment, the primer is extended by a polymerase to generate the complement of the template. In a further embodiment, the extended primer is separated from the template for use in a number of methods, including sequencing reactions. Methods of generating these compositions of matter are further provided.
摘要翻译： 本发明提供了一种双链体，其包含寡核苷酸引物和模板，其中引物与发色团或荧光团化学偶联，以允许通过聚合酶进行链延伸。 在一个实施方案中，引物通过聚合酶延伸以产生模板的补体。 在另一个实施方案中，将延伸的引物与模板分离以用于多种方法，包括测序反应。 进一步提供产生这些物质组合物的方法。
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公开(公告)号：US20090142761A1
公开(公告)日：2009-06-04
申请号：US12111008
申请日：2008-04-28
IPC分类号： C12Q1/68
CPC分类号： C12Q1/68 , C12Q1/6827 , C12Q1/6862 , C12Q2537/164 , C12Q2521/501
摘要： Ligation-based methods and kits are disclosed for determining the degree of methylation of one or more target nucleotides. In certain embodiments, the methylation status of one or more target nucleotides is determined by generating misligation products. In certain embodiments, at least one target nucleotide is amplified prior to the ligation reaction. In certain embodiments, at least one ligation product, at least one ligation product surrogate, at least one misligation product, at least one misligation product surrogate, or combinations thereof are amplified. In certain embodiments, one or more ligation probes comprise at least one nucleotide analog, at least one Modification, at least one mismatched nucleotide, or combinations thereof.
摘要翻译： 公开了基于连接的方法和试剂盒以确定一个或多个靶核苷酸的甲基化程度。 在某些实施方案中，通过产生错误产物来确定一个或多个靶核苷酸的甲基化状态。 在某些实施方案中，在连接反应之前扩增至少一种靶核苷酸。 在某些实施方案中，扩增至少一种连接产物，至少一种连接产物替代物，至少一种错误产物，至少一种错误产物替代物或其组合。 在某些实施方案中，一个或多个连接探针包含至少一个核苷酸类似物，至少一个修饰，至少一个错配核苷酸或其组合。
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