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93 条记录 (耗时 0.055 秒)

    Nucleic acids encoding artificial P-selectin ligands
    21.
    发明授权
    Nucleic acids encoding artificial P-selectin ligands 失效
    编码人工P-选择素配体的核酸

    公开(公告)号：US07399847B1

    公开(公告)日：2008-07-15

    申请号：US08756018

    申请日：1996-11-25

    申请人： Brian Seed ,  Tara Pouyani

    发明人： Brian Seed ,  Tara Pouyani

    IPC分类号： C07H21/04 ,  C12N1/20 ,  C12N15/00

    CPC分类号： C07K14/705

    摘要： Disclosed herein are organic molecules to which are covalently bonded a sialyl-Lex determinant and a sulfated determinant, at least one of these determinants being positioned at a non-naturally occurring site on the molecule. Also disclosed are particular P-selectin ligands and P-selectin ligand-antibody fusions. These molecules, ligands, and fusion proteins find use in methods of reducing or protecting against inflammation and extravasation-dependent adverse reactions, such as organ damage and clotting (for example, associated with adult respiratory distress syndrome or ischemic myocardial injury).

    摘要翻译： 本文公开的是共价键合唾液酸基 - 决定子和硫酸化决定簇的有机分子，这些决定簇中的至少一个位于分子上的非天然存在位点。 还公开了特定的P-选择素配体和P-选择素配体 - 抗体融合物。 这些分子，配体和融合蛋白可用于减少或保护免受炎症和外渗依赖性不良反应（如器官损伤和凝血）（例如与成人呼吸窘迫综合征或缺血性心肌损伤相关）的方法。

    Rapid mutational analysis method and compositions
    23.
    发明授权
    Rapid mutational analysis method and compositions 失效
    快速突变分析方法和组成

    公开(公告)号：US06579676B1

    公开(公告)日：2003-06-17

    申请号：US09400207

    申请日：1999-09-21

    申请人： Brian Seed ,  Andrew Peterson

    发明人： Brian Seed ,  Andrew Peterson

    IPC分类号： C12N1563

    CPC分类号： C12N15/1086 ,  C07K14/70514 ,  C12N15/1034 ,  G01N33/56972 ,  G01N33/56988 ,  G01N33/6878

    摘要： A novel rapid mutational analysis method for mapping protein epitopes is disclosed. This method has been used to identify the binding sites for 16 anti-CD2 and anti-CD4 monoclonal antibodies. The powerful, rapid, and simple method of the present invention allows isolation of a very large number of mutants, and is applicable to any intracellular or surface protein for which a cDNA and monoclonal antibodies are available. The present method is especially useful in ligand binding site studies for the design of new ligands and drugs.

    摘要翻译： 公开了一种用于测定蛋白质表位的新型快速突变分析方法。 该方法已被用于鉴定16种抗CD2和抗CD4单克隆抗体的结合位点。 本发明的强大，快速和简单的方法允许分离非常大量的突变体，并且可应用于可获得cDNA和单克隆抗体的任何细胞内或表面蛋白质。 本方法在配体结合位点研究中特别适用于新配体和药物的设计。

    Redirection of cellular immunity by protein tyrosine kinase chimeras
    24.
    发明授权
    Redirection of cellular immunity by protein tyrosine kinase chimeras 失效
    蛋白酪氨酸激酶嵌合体细胞免疫的重定向

    公开(公告)号：US06392013B1

    公开(公告)日：2002-05-21

    申请号：US08889712

    申请日：1997-07-07

    申请人： Brian Seed ,  Charles Romeo ,  Waldemar Kolanus

    发明人： Brian Seed ,  Charles Romeo ,  Waldemar Kolanus

    IPC分类号： C12P2102

    CPC分类号： C12N9/1205 ,  C07K14/705 ,  C07K14/70503 ,  C07K14/7051 ,  C07K14/70514 ,  C07K14/70535 ,  C07K19/00 ,  C07K2319/00

    摘要： Disclosed is a method of directing a cellular response in a mammal by expressing in a cell of the mammal a chimeric receptor which causes the cells to specifically recognize and destroy an infective agent, a cell infected with an infective agent, a tumor or cancerous cell, or an autoimmune-generated cell. The chimeric receptor includes an extracellular portion which is capable of specifically recognizing and binding the target cell or target infective agent, and (b) an intracellular portion of a protein-tyrosine kinase which is capable of signalling the therapeutic cell to destroy a receptor-bound target cell or a receptor-bound target infective agent. Also disclosed are cells which express the chimeric receptors and DNA encoding the chimeric receptors.

    摘要翻译： 公开了一种通过在哺乳动物细胞中表达嵌合受体来引导细胞反应的方法，所述嵌合受体导致细胞特异性识别和破坏感染剂，感染了感染剂，肿瘤或癌细胞的细胞， 或自身免疫产生的细胞。 嵌合受体包括能够特异性识别和结合靶细胞或靶感染剂的细胞外部分，和（b）蛋白质 - 酪氨酸激酶的细胞内部分，其能够表达治疗细胞以破坏受体结合 靶细胞或受体结合的靶感染剂。 还公开了表达嵌合受体的细胞和编码嵌合受体的DNA。

    Redirection of cellular immunity by protein tyrosine kinase chimeras
    26.
    发明授权
    Redirection of cellular immunity by protein tyrosine kinase chimeras 失效
    蛋白酪氨酸激酶嵌合体细胞免疫的重定向

    公开(公告)号：US6004811A

    公开(公告)日：1999-12-21

    申请号：US394912

    申请日：1995-02-24

    申请人： Brian Seed ,  Charles Romeo ,  Waldemar Kolanus

    发明人： Brian Seed ,  Charles Romeo ,  Waldemar Kolanus

    IPC分类号： A61K35/14 ,  A61K38/00 ,  C07K14/705 ,  C07K14/725 ,  C07K14/73 ,  C07K14/735 ,  C07K19/00 ,  C12N9/12 ,  C12P21/04 ,  C12N5/00 ,  C07H21/00 ,  C12N15/09

    CPC分类号： C12N9/1205 ,  C07K14/705 ,  C07K14/70503 ,  C07K14/7051 ,  C07K14/70514 ,  C07K14/70535 ,  C07K19/00 ,  C07K2319/00

    摘要： Disclosed is a method of directing a cellular response in a mammal by expressing in a cell of the mammal a chimeric receptor which causes the cells to specifically recognize and destroy an infective agent, a cell infected with an infective agent, a tumor or cancerous cell, or an autoimmune-generated cell. The chimeric receptor includes an extracellular portion which is capable of specifically recognizing and binding the target cell or target infective agent, and (b) an intracellular portion of a protein-tyrosine kinase which is capable of signalling the therapeutic cell to destroy a receptor-bound target cell or a receptor-bound target infective agent. Also disclosed are calls which express the chimeric receptors and DNA encoding the chimeric receptors.

    摘要翻译： 公开了一种通过在哺乳动物细胞中表达嵌合受体来引导细胞反应的方法，所述嵌合受体导致细胞特异性识别和破坏感染剂，感染了感染剂，肿瘤或癌细胞的细胞， 或自身免疫产生的细胞。 嵌合受体包括能够特异性识别和结合靶细胞或靶感染剂的细胞外部分，和（b）蛋白质 - 酪氨酸激酶的细胞内部分，其能够表达治疗细胞以破坏受体结合 靶细胞或受体结合的靶感染剂。 还公开了表达嵌合受体和编码嵌合受体的DNA的调用。

    Cloning vector, polylinker and methods
    28.
    发明授权
    Cloning vector, polylinker and methods 失效
    克隆载体，多接头和方法

    公开(公告)号：US5830731A

    公开(公告)日：1998-11-03

    申请号：US861205

    申请日：1997-05-21

    申请人： Brian Seed ,  Alejandro Aruffo

    发明人： Brian Seed ,  Alejandro Aruffo

    IPC分类号： A61K38/00 ,  C07K14/705 ,  C12N15/10 ,  C12N15/12 ,  C12N15/85 ,  G01N33/569 ,  C12N15/66

    CPC分类号： C07K14/705 ,  C07K14/70578 ,  C12N15/1034 ,  C12N15/85 ,  G01N33/56972 ,  A61K38/00 ,  C12N2800/108 ,  C12N2830/00 ,  C12N2830/15 ,  C12N2830/38 ,  C12N2830/60 ,  C12N2840/44

    摘要： A simple and highly efficient method for cloning cDNAs from mammalian expression libraries based on transient expression in mammalian host cells has been discovered. Novel expression vectors allowing highly efficient construction of mammalian cDNA libraries are disclosed. The cloning method of the invention which has been used to clone genes for cell surface antigens of human lymphocytes, has general application in gene cloning. Cell surface antigens cloned according to the present invention have been purified, and the nucleotide and amino acid sequences determined. These antigens have diagnostic and therapeutic utility in immune-mediated infections in mammals, including humans.

    摘要翻译： 已经发现了一种用于从哺乳动物宿主细胞中的瞬时表达克隆来自哺乳动物表达文库的cDNA的简单且高效的方法。 公开了能够高效构建哺乳动物cDNA文库的新型表达载体。 用于克隆人淋巴细胞表面抗原基因的本发明的克隆方法在基因克隆中具有一般应用。 已经纯化了根据本发明克隆的细胞表面抗原，并确定了核苷酸和氨基酸序列。 这些抗原在哺乳动物（包括人）的免疫介导感染中具有诊断和治疗效用。