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    Droplet Actuator Devices and Methods for Immunoassays and Washing
    22.
    发明申请
    Droplet Actuator Devices and Methods for Immunoassays and Washing 有权
    滴液致动器装置和免疫测定和洗涤方法

    公开(公告)号：US20090263834A1

    公开(公告)日：2009-10-22

    申请号：US12393534

    申请日：2009-02-26

    申请人： Ramakrishna Sista ,  Michael G. Pollack ,  Vamsee K. Pamula ,  Vijay Srinivasan

    发明人： Ramakrishna Sista ,  Michael G. Pollack ,  Vamsee K. Pamula ,  Vijay Srinivasan

    IPC分类号： G01N33/542

    CPC分类号： G01N33/54333

    摘要： Droplet actuator devices and methods for immunoassays and washing are provided. According to one embodiment, a method of providing a droplet in contact with a surface of a super paramagnetic bead with a reduced concentration of a substance is provided and includes: (a) providing a super paramagnetic bead in contact with a droplet comprising a starting concentration and starting quantity of the substance and having a starting volume; (b) conducting one or more droplet operations to merge a wash droplet with the droplet provided in step (a) to yield a combined droplet; and (c) conducting one or more droplet operations to divide the combined droplet to yield a set of droplets. The set of droplets includes: (i) a droplet in contact with the super paramagnetic bead having a decreased concentration of the substance relative to the starting concentration; and (ii) a droplet which is separated from the super paramagnetic bead.

    摘要翻译： 提供液滴致动器装置和免疫测定和洗涤方法。 根据一个实施方案，提供了一种提供与物质浓度降低的超顺磁珠的表面接触的液滴的方法，包括：（a）提供与包含起始浓度的液滴接触的超顺磁珠 并开始物质的起始体积; （b）进行一个或多个液滴操作以将洗涤液滴与步骤（a）中提供的液滴合并以产生组合液滴; 和（c）进行一个或多个液滴操作以分开组合液滴以产生一组液滴。 一组液滴包括：（i）与超顺磁珠接触的液滴，其物质相对于起始浓度降低; 和（ii）从超顺磁珠分离的液滴。

    Method of Detecting an Analyte
    26.
    发明申请
    Method of Detecting an Analyte 有权
    检测分析物的方法

    公开(公告)号：US20090311713A1

    公开(公告)日：2009-12-17

    申请号：US12546253

    申请日：2009-08-24

    申请人： Michael G. Pollack ,  Zhishan Hua ,  Allen E. Eckhardt ,  Prasanna Thwar ,  Vijay Srinivasan ,  Vamsee K. Pamula

    发明人： Michael G. Pollack ,  Zhishan Hua ,  Allen E. Eckhardt ,  Prasanna Thwar ,  Vijay Srinivasan ,  Vamsee K. Pamula

    IPC分类号： C12Q1/68

    CPC分类号： B01L3/502792 ,  B01L3/502753 ,  B01L3/502761 ,  B01L7/525 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2300/089 ,  B01L2400/0427 ,  B01L2400/043 ,  C12Q1/6848 ,  C12Q2565/629

    摘要： The invention relates to certain novel approaches to reducing or eliminating the movement of contaminants from one droplet to another on a droplet actuator via liquid filler fluid. In one application, a method of detecting an analyte is provided and includes providing in a detection window a droplet including a signal-producing substance indicative of the presence and/or quantity of an analyte and one or more magnetically responsive beads which may interfere with signal produced by the signal producing substance. The method further includes using a magnetic field for magnetically removing the magnetically responsive beads from the detection window and/or magnetically restricting the magnetically responsive beads from entering the detection window while transporting and/or retaining the droplet in the detection window. The method additionally includes detecting a signal produced by the signal-producing substance.

    摘要翻译： 本发明涉及在液滴致动器上通过液体填充液减少或消除污染物从一个液滴到另一个液滴的移动的某些新颖方法。 在一个应用中，提供检测分析物的方法，并且包括在检测窗口中提供包含指示分析物的存在和/或量的信号产生物质的液滴和一个或多个可能干扰信号的磁响应珠子 由信号产生物质产生。 该方法还包括使用磁场来从检测窗口磁性地去除磁响应珠子和/或磁力限制磁响应珠子进入检测窗口，同时在检测窗口中传送和/或保持液滴。 该方法还包括检测由信号产生物质产生的信号。