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1.发明申请Droplet Actuator Configurations and Methods of Conducting Droplet Operations 审中-公开液滴执行器配置和执行液滴操作的方法公开(公告)号：US20100270156A1
公开(公告)日：2010-10-28
申请号：US12682830
申请日：2008-12-23
申请人： Vijay Srinivasan , Michael G. Pollack , Vamsee K. Pamula , Zhishan Hua , Arjun Sudarsan , Philip Paik
发明人： Vijay Srinivasan , Michael G. Pollack , Vamsee K. Pamula , Zhishan Hua , Arjun Sudarsan , Philip Paik
IPC分类号： C07K1/26
CPC分类号： B01L3/52 , B01F13/0071 , B01F13/0076 , B01L3/0241 , B01L3/502792 , B01L2200/027 , B01L2200/0605 , B01L2200/143 , B01L2200/16 , B01L2300/0816 , B01L2300/0819 , B01L2300/089 , B01L2400/0427 , B01L2400/06 , B01L2400/0688 , F04B19/006
摘要： A droplet actuator with a droplet formation electrode configuration associated with a droplet operations surface, wherein the electrode configuration comprises one or more electrodes configured to control volume of a droplet during formation of a sub-droplet on the droplet operations surface. Methods of making and using the droplet actuator are also provided.
摘要翻译： 具有与液滴操作表面相关联的液滴形成电极配置的液滴致动器，其中所述电极配置包括一个或多个电极，其被配置为在液滴操作表面上形成子液滴期间控制液滴的体积。 还提供了制造和使用液滴致动器的方法。
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公开(公告)号：US09675972B2
公开(公告)日：2017-06-13
申请号：US12921256
申请日：2009-03-09
申请人： Michael G. Pollack , Ramakrishna Sista , Zhishan Hua , Vamsee K. Pamula , Arjun Sudarsan , Vijay Srinivasan , Prasanna Thwar , Alexander Shenderov , Dwayne Allen
发明人： Michael G. Pollack , Ramakrishna Sista , Zhishan Hua , Vamsee K. Pamula , Arjun Sudarsan , Vijay Srinivasan , Prasanna Thwar , Alexander Shenderov , Dwayne Allen
CPC分类号： B01L3/0282 , B01F13/002 , B01F13/0023 , B01F13/0071 , B01F13/0076 , B01F13/0818 , B01L3/502753 , B01L2200/0621 , B01L2200/0631 , B01L2200/0652 , B01L2200/0668 , B01L2200/141 , B01L2300/0832 , B01L2300/0864 , B01L2300/0867 , B01L2400/043 , B03C1/01 , B03C1/284 , B03C1/286 , B03C2201/18 , B03C2201/26 , B03C2201/28 , Y10T436/25
摘要： Methods of concentrating beads in a droplet and/or loading beads on a fluidic device are provided, including among other things, a method of concentrating beads in a droplet, the method comprising: (a) providing a droplet actuator comprising: (i) an interior droplet operations volume; and (ii) a reservoir exterior to the interior volume; (iii) a droplet established in a liquid path extending from the reservoir into the interior volume; (b) providing magnetically responsive beads in the portion of the droplet which is in the reservoir; (c) magnetically attracting the magnetically responsive beads through the liquid path into the portion of the droplet which is in the interior volume; and (d) forming a droplet comprising one or more of the magnetically responsive beads in the interior volume.
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公开(公告)号：US08846414B2
公开(公告)日：2014-09-30
申请号：US12569725
申请日：2009-09-29
申请人： Ramakrishna Sista , Vamsee K. Pamula , Arjun Sudarsan , Vijay Srinivasan , Michael G. Pollack , Richard B. Fair , Allen E. Eckhardt
发明人： Ramakrishna Sista , Vamsee K. Pamula , Arjun Sudarsan , Vijay Srinivasan , Michael G. Pollack , Richard B. Fair , Allen E. Eckhardt
IPC分类号： G01N33/553 , G01N33/68 , G01N33/543
CPC分类号： G01N33/6893 , G01N33/54366 , G01N2333/4712 , G01N2333/58 , G01N2800/32 , Y10T436/25
摘要： The present invention provides for the detection of cardiac markers on a droplet actuator. An aspect provides a method of assaying a cardiac marker in a biological sample from a subject, the method including providing a droplet actuator, loading the biological sample and assay reagents on the droplet actuator, executing droplet operations to create sample droplets from the sample and reagent droplets from the reagents on the droplet actuator, and executing droplet operations using the sample droplets and reagent droplets to produce a detectable signal indicative of the quantity of the cardiac marker in the biological sample. Still other aspects are provided.
摘要翻译： 本发明提供了在液滴致动器上检测心脏标志物。 一方面提供了从受试者的生物样品中测定心脏标志物的方法，所述方法包括提供液滴致动器，在液滴致动器上装载生物样品和测定试剂，执行液滴操作以从样品和试剂产生样品液滴 来自液滴致动器上的试剂的液滴，以及使用样品液滴和试剂液滴执行液滴操作，以产生指示生物样品中心脏标志物量的可检测信号。 还提供了其他方面。
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公开(公告)号：US08685344B2
公开(公告)日：2014-04-01
申请号：US12523776
申请日：2008-01-22
CPC分类号： B01L3/0241 , B01F13/0071 , B01F13/0076 , B01L3/502792 , B01L2300/0819 , B01L2300/089 , B01L2300/165 , B01L2400/0415 , B01L2400/0427
摘要： The present invention relates to surface assisted fluid loading and droplet dispensing on a droplet micro actuator. A droplet actuator is provided and includes one or more electrodes configured for conducting one or more droplet operations on a droplet operations surface of the substrate. The droplet actuator further includes a wettable surface defining a path from a fluid reservoir into a locus which is sufficiently near to one or more of the electrodes that activation of the one or more electrodes results in a droplet operation. Methods and systems are also provided.
摘要翻译： 本发明涉及液滴微型致动器上的表面辅助流体加载和液滴分配。 提供液滴致动器并且包括一个或多个电极，其被配置用于在基板的液滴操作表面上进行一个或多个液滴操作。 液滴致动器还包括可润湿表面，其限定从流体储存器到足以接近一个或多个电极的轨迹的路径，所述一个或多个电极的激活导致液滴操作。 还提供了方法和系统。
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公开(公告)号：US08562807B2
公开(公告)日：2013-10-22
申请号：US12747231
申请日：2008-12-10
申请人： Vijay Srinivasan , Michael G. Pollack , Zhishan Hua , Arjun Sudarsan , Allen E. Eckhardt , Vamsee K. Pamula
发明人： Vijay Srinivasan , Michael G. Pollack , Zhishan Hua , Arjun Sudarsan , Allen E. Eckhardt , Vamsee K. Pamula
IPC分类号： G01N27/453
CPC分类号： G01N27/44743 , B01L3/0268 , B01L3/502761 , B01L3/502792 , B01L2200/0605 , B01L2200/061 , B01L2200/0647 , B01L2300/0645 , B01L2300/16 , B01L2400/0415 , B01L2400/0427
摘要： Droplet actuators for conducting droplet operations, such as droplet transport and droplet dispensing, are provided. In one embodiment, the droplet actuator may include a substrate including, droplet operations electrodes arranged for conducting droplet operations on a surface of the substrate; and reference electrodes associated with the droplet operations electrodes and extending above the surface of the substrate. Other embodiments of droplet actuators and methods of loading and using such droplet actuators are also provided.
摘要翻译： 提供用于进行液滴操作的滴液致动器，例如液滴传输和液滴分配。 在一个实施例中，液滴致动器可以包括基板，其包括布置成在基板的表面上进行液滴操作的液滴操作电极; 以及与液滴操作电极相关并在衬底表面上方延伸的参考电极。 还提供了液滴致动器的其他实施例以及加载和使用这种液滴致动器的方法。
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公开(公告)号：US20110104747A1
公开(公告)日：2011-05-05
申请号：US12921256
申请日：2009-03-09
申请人： Michael G. Pollack , Ramakrishna Sista , Zhishan Hua , Vamsee K. Pamula , Arjun Sudarsan , Vijay Srinivasan , Prasanna Thwar , Alexander Shenderov , Dwayne Allen
发明人： Michael G. Pollack , Ramakrishna Sista , Zhishan Hua , Vamsee K. Pamula , Arjun Sudarsan , Vijay Srinivasan , Prasanna Thwar , Alexander Shenderov , Dwayne Allen
IPC分类号： G01N1/40
CPC分类号： B01L3/0282 , B01F13/002 , B01F13/0023 , B01F13/0071 , B01F13/0076 , B01F13/0818 , B01L3/502753 , B01L2200/0621 , B01L2200/0631 , B01L2200/0652 , B01L2200/0668 , B01L2200/141 , B01L2300/0832 , B01L2300/0864 , B01L2300/0867 , B01L2400/043 , B03C1/01 , B03C1/284 , B03C1/286 , B03C2201/18 , B03C2201/26 , B03C2201/28 , Y10T436/25
摘要： Methods of concentrating beads in a droplet and/or loading beads on a fluidic device are provided, including among other things, a method of concentrating beads in a droplet, the method comprising: (a) providing a droplet actuator comprising: (i) an interior droplet operations volume; and (ii) a reservoir exterior to the interior volume; (iii) a droplet established in a liquid path extending from the reservoir into the interior volume; (b) providing magnetically responsive beads in the portion of the droplet which is in the reservoir; (c) magnetically attracting the magnetically responsive beads through the liquid path into the portion of the droplet which is in the interior volume; and (d) forming a droplet comprising one or more of the magnetically responsive beads in the interior volume.
摘要翻译： 提供了将珠子集中在流体装置上的液滴和/或负载珠粒中的方法，其中包括将珠粒集中在液滴中的方法，所述方法包括：（a）提供液滴致动器，其包括：（i） 内部液滴操作量; 和（ii）内部容积外部的储存器; （iii）建立在从储存器延伸到内部容积中的液体路径中的液滴; （b）在储存器中的液滴部分中提供磁响应珠; （c）通过所述液体路径将所述磁响应珠磁吸引到位于所述内部容积中的所述液滴的所述部分中; 和（d）在内部容积中形成包含一个或多个磁响应珠的液滴。
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公开(公告)号：US20090304944A1
公开(公告)日：2009-12-10
申请号：US12523776
申请日：2008-01-22
CPC分类号： B01L3/0241 , B01F13/0071 , B01F13/0076 , B01L3/502792 , B01L2300/0819 , B01L2300/089 , B01L2300/165 , B01L2400/0415 , B01L2400/0427
摘要： The present invention relates to surface assisted fluid loading and droplet dispensing on a droplet micro actuator. A droplet actuator is provided and includes one or more electrodes configured for conducting one or more droplet operations on a droplet operations surface of the substrate. The droplet actuator further includes a wettable surface defining a path from a fluid reservoir into a locus which is sufficiently near to one or more of the electrodes that activation of the one or more electrodes results in a droplet operation. Methods and systems are also provided.
摘要翻译： 本发明涉及液滴微型致动器上的表面辅助流体加载和液滴分配。 提供液滴致动器并且包括一个或多个电极，其被配置用于在基板的液滴操作表面上进行一个或多个液滴操作。 液滴致动器还包括可润湿表面，其限定从流体储存器到足以接近一个或多个电极的轨迹的路径，所述一个或多个电极的激活导致液滴操作。 还提供了方法和系统。
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公开(公告)号：US08852952B2
公开(公告)日：2014-10-07
申请号：US12990815
申请日：2009-05-04
申请人： Michael G. Pollack , Prasanna Thwar , Vamsee K. Pamula , Allen Eckhardt , Alexander Shenderov , Dwayne Allen , Vijay Srinivasan
发明人： Michael G. Pollack , Prasanna Thwar , Vamsee K. Pamula , Allen Eckhardt , Alexander Shenderov , Dwayne Allen , Vijay Srinivasan
IPC分类号： G01N1/00
CPC分类号： B01L3/502784 , B01L3/502715 , B01L3/502761 , B01L7/52 , B01L2200/027 , B01L2200/0673 , B01L2300/022 , B01L2300/023 , B01L2300/024 , B01L2300/0672 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2400/0415 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0478 , B01L2400/0481 , B01L2400/0487 , B01L2400/049 , B01L2400/0683 , G01N27/44704 , G01N35/0098 , G01N2035/1046 , Y10T436/25 , Y10T436/2575
摘要： The invention provides droplet actuators and droplet actuator cassettes including reagent storage capabilities, as well as methods of making and using the droplet actuators and cassettes. The invention also provides continuous flow channel elements and techniques for using electrodes to manipulate droplets in flowing streams. The invention also discloses methods of separating compounds on a droplet actuator. Various other aspects of the invention are also disclosed.
摘要翻译： 本发明提供了包括试剂储存能力的液滴致动器和液滴致动器盒，以及制造和使用液滴致动器和盒的方法。 本发明还提供了用于使用电极在流动流中操纵液滴的连续流动通道元件和技术。 本发明还公开了在液滴致动器上分离化合物的方法。 还公开了本发明的各种其它方面。
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公开(公告)号：US08809068B2
公开(公告)日：2014-08-19
申请号：US12761066
申请日：2010-04-15
IPC分类号： G01N15/06
CPC分类号： G01N33/54326 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01F13/0809 , B01L3/50273 , B01L3/502761 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0647 , B01L2200/0673 , B01L2300/06 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0421 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , G01N1/38 , G01N2001/386 , G01N2035/1046 , Y10T436/11 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
摘要： The invention provides a method of dispersing or circulating magnetically responsive beads within a droplet in a droplet actuator. The invention, in one embodiment, makes use of a droplet actuator with a plurality of droplet operations electrodes configured to transport the droplet, and a magnetic field present at a portion of the plurality of droplet operations electrodes. A bead-containing droplet is provided on the droplet actuator in the presence of the uniform magnetic field. Beads are circulated in the droplet during incubation by conducting droplet operations on the droplet within a uniform region of the magnetic field wherein droplet operations do not allow magnetically responsive beads to be introduced into a region of the magnetic field which is sufficiently non-uniform to cause bead clumping resulting in a more homogenous distribution of the beads in the droplet. Other embodiments are also provided.
摘要翻译： 本发明提供了一种在液滴致动器中的液滴内分散或循环磁响应珠的方法。 在一个实施例中，本发明利用具有配置成传送液滴的多个液滴操作电极的液滴致动器和存在于多个液滴操作电极的一部分处的磁场。 在均匀磁场存在的情况下，在液滴致动器上提供含珠的液滴。 在孵育期间，通过在磁场的均匀区域内对液滴进行液滴操作，珠在液滴中循环，其中液滴操作不允许将磁响应的珠引入磁场的区域，该区域足够不均匀，从而导致 珠粒聚集，导致珠粒在液滴中更均匀的分布。 还提供了其他实施例。
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公开(公告)号：US20130293246A1
公开(公告)日：2013-11-07
申请号：US13988190
申请日：2011-11-15
CPC分类号： G01N27/221 , B01L3/502784 , B01L3/502792 , B01L2200/14 , B01L2300/0645 , B01L2300/0816 , B01L2300/089 , B01L2400/0427 , B41J2/04541 , B41J2/04555 , B41J2/0458 , G01K7/34
摘要： The embodiments described herein provide methods of measuring capacitance, detecting a droplet at a position, determining a thickness of an oil film and determining temperature in a droplet actuator. Specifically, the capacitance detection method may be used as a real-time verification tool in order to detect the absence, presence, and/or partial presence of a droplet at an electrode, analyze droplet properties, measure droplet size or volume, optimize the speed of droplet operation and detect air bubbles.
摘要翻译： 本文所述的实施例提供了测量电容，检测位置处的液滴，确定油膜的厚度和确定液滴致动器中的温度的方法。 具体地，可以将电容检测方法用作实时验证工具，以便检测电极处的液滴的不存在，存在和/或部分存在，分析液滴属性，测量液滴尺寸或体积，优化速度 的液滴操作并检测气泡。
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