摘要:
Methods are provided for substantially reducing background signals encountered in nucleic acid hybridization assays. The method is premised on the elimination or significant reduction of the phenomenon of nonspecific hybridization, so as to provide a detectable signal which is produced only in the presence the target polynucleotide of interest. In addition, a novel method for the chemical synthesis of isoguanosine or 2'-deoxy-isoguanosine is provided. The invention also has applications in antisense and aptamer therapeutics and drug discovery.
摘要:
Linear or branched oligonucleotide multimers useful as amplifiers in biochemical assays which comprise (1) at least one first single-stranded oligonucleotide unit that is complementary to a single-stranded oligonucleotide sequence of interest, and (2) a multiplicity of second single-stranded oligonucleotide units that are complementary to a single-stranded labeled oligonucleotide. Amplified sandwich nucleic acid hybridizations and immunoassays using the multimers are exemplified.
摘要:
Novel N-4 modified pyrimidine analogs are provided having the structure (I) or (II) ##STR1## wherein: R.sup.1 is selected from the group consisting of hydrogen, acid-sensitive, base-stable blocking groups and acyl capping groups; R.sup.2 is lower alkyl; R.sup.3 is C.sub.1 -C.sub.12 alkylene containing 0 to 6 linkages selected from the group consisting of --0--, --S-- and --NH--; R.sup.4 is ##STR2## in which R.sup.9 is hydrogen or an optionally substituted aliphatic group; R.sup.10 and R.sup.11 are hydrocarbyl or together form a mono- or polyheterocyclic ring; R.sup.5 is hydrogen or lower alkyl; R.sup.6 is selected from the group consisting of hydrogen, methyl, bromo and iodo; R.sup.7 is C.sub.1 -C.sub.12 alkylene containing 0 to 6 linkages selected from the group consisting of --O--, --S-- and --NR.sup.12 -- wherein R.sup.12 is hydrogen or lower alkyl; and R.sup.8 is a protecting group that can be removed and replaced by reduction. Synthetic methods for preparing the compounds are provided as well, as are methods for making and using polynucleotide probes containing the novel pyrimidine analogs.
摘要翻译:提供具有结构(I)或(II)的新型N-4修饰的嘧啶类似物,其中:R1选自氢,酸敏感,碱稳定 封闭基团和酰基封端基团; R2是低级烷基; R3是含有0-6个选自-O - , - S - 和-NH-的键的C1-C12亚烷基; R4是,其中R9是氢或任选取代的脂族基; R 10和R 11是烃基或一起形成单或多杂环; R5是氢或低级烷基; R 6选自氢,甲基,溴和碘; R7是含有0-6个选自-O - , - S-和-NR12-的键的C1-C12亚烷基,其中R12是氢或低级烷基; R8是可以除去并被还原代替的保护基。 还提供了制备化合物的合成方法,以及制备和使用含有新型嘧啶类似物的多核苷酸探针的方法。
摘要:
Novel reagents useful in a variety of biochemical and chemical contexts, including nucleic hybridization assays and chemical phosphorylation of hydroxyl-containing compounds. The reagents are particularly useful for introducing cleavable sites and/or abasic sites into oligonucleotide or polynucleotide chains.
摘要:
Polynucleotides containing abasic, cleavable sites are provided. These polynucleotides are useful in a variety of biochemical and chemical contexts, particularly in solid phase nucleic acid hybridization assays because a captured probe can be released from the support. The polynucleotides have the structure ##STR1## where R is selected from the group consisting of 2-nitrobenzyl, 4-penten-1-yl, ##STR2## where R', R.sub.i and R.sub.j are as defined herein. One of the preferred embodiments is a polynucleotide where R is ##STR3##
摘要:
Linear or branched oligonucleotide multimers useful as amplifiers in biochemical assays which comprise (1) at least one first single-stranded oligonucleotide unit that is complementary to a single-stranded oligonucleotide sequence of interest, and (2) a multiplicity of second single-stranded oligonucleotide units that are complementary to a single-stranded labeled oligonucleotide. Amplified sandwich nucleic acid hybridizations and immunoassays using the multimers are exemplified.
摘要:
Disclosed herein are compounds that activate RNaseL, methods of synthesizing compounds that activate RNaseL and the use of compounds that activate RNaseL for treating and/or ameliorating a disease or a condition, such as a viral infection, a bacterial infection, cancer and/or parasitic disease.
摘要:
A system for and a method of monitoring a plurality of transceivers is disclosed. In one embodiment the method includes enabling one transceiver of the plurality of transceivers. The method also includes disabling all other transceivers of the plurality of transceivers. A transmit coil emits a high frequency electromagnetic field, the one transceiver transmitting a signal in response to the field of the transmit coil. At least one of a plurality of reader coils receives the signal transmitted by the one transceiver, the plurality of reader coils being suitably arranged in the vicinity of the area to be monitored. An evaluator determines a position of the one transceiver from the signal received from the one transceiver by the at least one of the plurality of reader coils.
摘要:
A nonwoven web is provided. The nonwoven web contains a side-by-side composite fiber having crimps. The composite fiber contains first and second propylene polymers. The nonwoven web contains a bonded portion that contains a bonding pattern containing zigzag unit patterns. The zigzag unit patterns are continuous and substantially parallel with a first direction and are disposed in a second direction at predetermined intervals. A triangle formed by adjacent three contact points of a first diagonal line and a second diagonal line of the unit pattern contains a part of the unit pattern that is adjacent to the triangle in the second direction.