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公开(公告)号：US08058033B2
公开(公告)日：2011-11-15
申请号：US12292563
申请日：2008-11-20
CPC分类号： C12N9/2428 , C12P7/06 , C12P19/02 , C12P19/14 , Y02E50/17
摘要： The present disclosure relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present disclosure provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions and cleaning compositions. The disclosure also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
摘要翻译： 本公开涉及具有改变的性质（例如改善的热稳定性和/或比活性）的母体葡糖淀粉酶的变体。 特别地，本公开提供了包含变体葡糖淀粉酶的组合物，包括淀粉水解组合物和清洁组合物。 本公开还涉及编码在宿主细胞中产生葡糖淀粉酶变体的变体和方法的DNA构建体。
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22.发明申请公开(公告)号：US20050084868A1
公开(公告)日：2005-04-21
申请号：US10688255
申请日：2003-10-16
IPC分类号： C07K14/705 , C12N9/86 , C12N15/10 , C12Q1/68 , C12P19/34
CPC分类号： C12N9/86 , C07K14/70503 , C12N15/102 , C12Y305/02006
摘要： The present invention provides methods and compositions for the production of stabilized proteins. In particular, the present invention provides methods and compositions for the generation of combinatorial libraries of consensus mutations and screening for improved protein variants.
摘要翻译： 本发明提供了用于生产稳定化蛋白质的方法和组合物。 特别地，本发明提供了用于产生共有突变的组合文库和筛选改良的蛋白质变体的方法和组合物。
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公开(公告)号：US08551755B2
公开(公告)日：2013-10-08
申请号：US12680193
申请日：2008-04-08
申请人： Wolfgang Aehle , Richard R. Bott , Igor Nikolaev , Martijn Scheffers , Piet Van Solingen , Casper Vroemen
发明人： Wolfgang Aehle , Richard R. Bott , Igor Nikolaev , Martijn Scheffers , Piet Van Solingen , Casper Vroemen
CPC分类号： C12N9/2428 , C12N15/09 , C12Y302/01003 , Y02E50/17
摘要： The present invention relates to glucoamylase variants. In particular, the invention relates to variants in the starch binding domain (SBD) of a glucoamylase. The invention also relates to variants having altered properties (e.g., improved thermostability and/or increased specific activity) as compared to a corresponding parent glucoamylase. The present invention also provides enzyme compositions comprising the variant glucoamylases; DNA constructs comprising polynucleotides encoding the variants; and methods of producing the glucoamylase variants in host cells.
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24.发明申请Variants of An Alpha-Amylase with Improved Production Levels in Fermentation Processes 有权在发酵过程中提高生产水平的α-淀粉酶的变体公开(公告)号：US20110027252A1
公开(公告)日：2011-02-03
申请号：US12601759
申请日：2008-05-29
申请人： Wolfgang Aehle , Neelam S. Amin
发明人： Wolfgang Aehle , Neelam S. Amin
IPC分类号： A01N63/02 , C07H21/04 , C12N1/21 , C12N15/63 , C12N9/26 , C12N9/98 , C12P19/20 , A01P1/00 , C12N9/16 , C12N9/08 , C12N9/20 , C12N9/22 , C11D3/386 , D06M16/00 , A23L1/0522 , B08B3/04
CPC分类号： C11D3/38618 , A21D8/042 , C11D3/386 , C12N9/2417 , C12P21/02 , Y02P20/52
摘要： Variants of Bacillus sp. no. 707 alpha amylase are provided that are produced more efficiently and thus more economically. Higher fermentation yields are achieved through introducing amino acid variations that promote solubility of the variant in a fermentation broth. Increased solubility allows more enzyme to remain in solution after expression in a host cell. This in turn increases the efficiency with which the expressed variant enzyme can be recovered from the fermentation broth.
摘要翻译： 芽孢杆菌的变种 没有。 提供707α淀粉酶，其更有效地并因此更经济地产生。 通过引入促进变体在发酵液中的溶解度的氨基酸变异来实现更高的发酵产率。 增加的溶解度允许在宿主细胞中表达后更多的酶保留在溶液中。 这又提高了从发酵液中回收表达的变体酶的效率。
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25.发明授权Amylolytic enzymes derived from the B.licheniformis α-amylase, having improved characteristics 失效衍生自地衣芽孢杆菌α-淀粉酶的淀粉分解酶具有改善的特征公开(公告)号：US06939703B2
公开(公告)日：2005-09-06
申请号：US10199922
申请日：2002-07-18
IPC分类号： C11D3/386 , C12N1/21 , C12N9/28 , C12N15/56 , C12N15/75 , C12P19/14 , D06L1/14 , D06M16/00 , D06M101/04 , C12N9/26 , C12N9/54 , C12Q1/40
CPC分类号： C12N9/2417 , C11D3/386 , D06L1/14 , Y02E50/17
摘要： The present invention relates to novel amylolytic enzymes having improved characteristics for the use in starch degradation, in textile or paper desizing and in household detergent compositions. The disclosed α-amylases show surprisingly improved properties with respect to the activity level and the combination of thermostability and a higher activity level. These improved properties make them more suitable for the use under more acidic or more alkaline conditions. The improved properties allow also the reduction of the Calcium concentration under application conditions without a loss of performance of the enzyme.
摘要翻译： 本发明涉及具有改进的淀粉降解特性，纺织品或纸浆脱浆和家用洗涤剂组合物中的新型淀粉分解酶。 所公开的α-淀粉酶在活性水平以及热稳定性和较高活性水平的组合方面表现出惊人的改善的性质。 这些改进的性能使它们更适合在更酸性或更碱性的条件下使用。 改进的性质还允许在施用条件下降低钙浓度而不损失酶的性能。
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公开(公告)号：US06217942B1
公开(公告)日：2001-04-17
申请号：US09207289
申请日：1998-12-08
申请人： Rob Bolle , Wolfgang Aehle
发明人： Rob Bolle , Wolfgang Aehle
IPC分类号： B05D300
CPC分类号： C09D197/005
摘要： A method is provided for producing a coated article comprising: (a) preparing a solution of lignin; (b) mixing the lignin solution with a phenol oxidizing enzyme; (c) incubating the mixture from said step (b) under conditions and for a time sufficient to form a solution of a desired viscosity; (d) contacting or spreading the mixture from the step (c) on an article to be coated; and (e) allowing the coating to set onto the article by subjecting the article to conditions and for a time sufficient to form a coating on the surface of the article.
摘要翻译： 提供了一种制备涂覆制品的方法，包括：（a）制备木质素溶液; （b）将木质素溶液与酚氧化酶混合; （c）将所述步骤（b）的混合物在足以形成所需粘度的溶液的条件下培养一段时间; （d）将来自步骤（c）的混合物接触或铺展在待涂覆的制品上; 和（e）通过使制品经受制品的表面上足以形成涂层的条件和时间使涂层固定在制品上。
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公开(公告)号：US09447397B2
公开(公告)日：2016-09-20
申请号：US12443255
申请日：2007-10-09
CPC分类号： C12N9/2428 , C12N15/09 , C12Y302/01003 , Y02E50/17
摘要： The present invention relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present invention provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions, animal feed compositions and cleaning compositions. The invention also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
摘要翻译： 本发明涉及具有改变的性质（例如改善的热稳定性和/或比活性）的母体葡糖淀粉酶的变体。 特别地，本发明提供包含变体葡糖淀粉酶，包括淀粉水解组合物，动物饲料组合物和清洁组合物的组合物。 本发明还涉及编码在宿主细胞中产生葡糖淀粉酶变体的变体和方法的DNA构建体。
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公开(公告)号：US08679792B2
公开(公告)日：2014-03-25
申请号：US13251116
申请日：2011-09-30
CPC分类号： C12N9/2428 , C12P7/06 , C12P19/02 , C12P19/14 , Y02E50/17
摘要： The present disclosure relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present disclosure provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions and cleaning compositions. The disclosure also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
摘要翻译： 本公开涉及具有改变的性质（例如改善的热稳定性和/或比活性）的母体葡糖淀粉酶的变体。 特别地，本公开提供了包含变体葡糖淀粉酶的组合物，包括淀粉水解组合物和清洁组合物。 本公开还涉及编码在宿主细胞中产生葡糖淀粉酶变体的变体和方法的DNA构建体。
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公开(公告)号：US20130309726A9
公开(公告)日：2013-11-21
申请号：US13806398
申请日：2011-06-30
申请人： Peter Edvard Degn , Richard R. Bott , Casper Willem Vroemen , Martijn Silvan Scheffers , Wolfgang Aehle , Elin Petersen
发明人： Peter Edvard Degn , Richard R. Bott , Casper Willem Vroemen , Martijn Silvan Scheffers , Wolfgang Aehle , Elin Petersen
CPC分类号： C12N9/2408 , A23L29/35 , C12C5/004 , C12C7/04 , C12N9/2428 , C12P19/02 , C12P19/20 , C12Y302/01003 , C13K1/06 , Y02P20/52
摘要： The present invention relates to combinatorial variants of a parent glucoamylase that have altered properties for reducing the synthesis of condensation products during hydrolysis of starch. Accordingly the variants of a parent glucoamylase are suitable such as for use within brewing and glucose syrup production. Also disclosed are DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
摘要翻译： 本发明涉及母体葡糖淀粉酶的组合变体，其在淀粉水解期间具有降低缩合产物合成的性质。 因此，母体葡糖淀粉酶的变体是适合的，例如用于酿造和葡萄糖浆生产中。 还公开了编码在宿主细胞中产生葡糖淀粉酶变体的变体和方法的DNA构建体。
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30.发明授权Variants of an alpha-amylase with improved production levels in fermentation processes 有权在发酵过程中提高生产水平的α-淀粉酶的变体公开(公告)号：US08546121B2
公开(公告)日：2013-10-01
申请号：US12601759
申请日：2008-05-29
申请人： Wolfgang Aehle , Neelam S. Amin
发明人： Wolfgang Aehle , Neelam S. Amin
IPC分类号： C12N9/26
CPC分类号： C11D3/38618 , A21D8/042 , C11D3/386 , C12N9/2417 , C12P21/02 , Y02P20/52
摘要： Variants of Bacillus sp. no. 707 alpha amylase are provided that are produced more efficiently and thus more economically. Higher fermentation yields are achieved through introducing amino acid variations that promote solubility of the variant in a fermentation broth. Increased solubility allows more enzyme to remain in solution after expression in a host cell. This in turn increases the efficiency with which the expressed variant enzyme can be recovered from the fermentation broth.
摘要翻译： 芽孢杆菌的变种 没有。 提供707α淀粉酶，其更有效地并因此更经济地产生。 通过引入促进变体在发酵液中的溶解度的氨基酸变异来实现更高的发酵产率。 增加的溶解度允许在宿主细胞中表达后更多的酶保留在溶液中。 这又提高了从发酵液中回收表达的变体酶的效率。
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