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    Methods of analyzing capped ribonucleic acids
    22.
    发明授权
    Methods of analyzing capped ribonucleic acids 有权

    公开(公告)号：US12195794B2

    公开(公告)日：2025-01-14

    申请号：US17056378

    申请日：2019-05-22

    Applicant: The Regents of the University of California

    Inventor： Logan Mulroney ,  Mark Akeson ,  Miten Jain ,  Hugh Olsen

    IPC: C12Q1/6869 ,  C12P19/34

    Abstract: Provided are methods of analyzing capped ribonucleic acids (RNAs). The methods include translocating an adapted RNA through a nanopore of a nanopore device. The adapted RNA includes an RNA region, a 5′ cap, and an adapter polynucleotide attached to the 5′ cap. The methods include monitoring ionic current through the nanopore during the translocating, translocating the 5′ cap through the nanopore, and identifying one or more ionic current features characteristic of the 5′ cap (e.g., a triphosphate linkage between the 5′ cap and nucleotide N1 of the RNA region, a 5′ to 5′ orientation of the 5′ cap and nucleotide N1 of the RNA region, and/or the like), translocating through the nanopore. Also provided are computer-readable media, computer devices, and systems that find use, e.g., in practicing the methods of the present disclosure.

    NUCLEIC ACID SEQUENCING SYSTEM
    25.
    发明申请
    NUCLEIC ACID SEQUENCING SYSTEM 有权

    公开(公告)号：US20250011864A1

    公开(公告)日：2025-01-09

    申请号：US18887904

    申请日：2024-09-17

    Applicant: ILLUMINA, INC.

    Inventor： Robert C. Kain ,  David L. Heiner ,  Chanfeng Zhao ,  Kevin Gunderson

    IPC: C12Q1/6874 ,  C12Q1/686 ,  C12Q1/6869

    Abstract: A system for sequencing nucleic acid comprising a plurality of stations and a system control. The system control configured to direct the first substrate to progress and retrogress between the first processing station and the imaging station, direct the second substrate to progress and retrogress between the second processing station and the imaging station, and direct a chemistry cycle of a first sequencing procedure to occur within one of the first processing station or the second processing station while an imaging cycle of a second sequencing procedure occurs within the imaging station.

    DETECTION OF GENETIC OR MOLECULAR ABERRATIONS ASSOCIATED WITH CANCER
    27.
    发明申请
    DETECTION OF GENETIC OR MOLECULAR ABERRATIONS ASSOCIATED WITH CANCER 有权

    公开(公告)号：US20250006299A1

    公开(公告)日：2025-01-02

    申请号：US18674702

    申请日：2024-05-24

    Applicant: The Chinese University of Hong Kong

    Inventor： Yuk-Ming Dennis Lo ,  Kwan Chee Chan ,  Rossa Wai Kwun Chiu ,  Peiyong Jiang

    IPC: G16B20/20 ,  C12Q1/6809 ,  C12Q1/6869 ,  C12Q1/6886 ,  G16B20/00 ,  G16B20/10 ,  G16B30/00 ,  G16B30/10

    Abstract: Systems, apparatus, and methods are provided for determining aberrations in a biological sample from an organism. Biological samples including cell-free DNA fragments are analyzed to identify imbalances in chromosomal regions, e.g., due to deletions and/or amplifications in a tumor. Multiple loci are used for each chromosomal region. Imbalances can be used to diagnose a patient for cancer, prognosticate a patient with cancer, or to detect the presence or monitor progress of a premalignant condition. The severity of an imbalance as well as the number of regions exhibiting an imbalance can be used. A systematic analysis of non-overlapping segments of a genome can provide a general screening tool for a sample. Additionally, a patient can be tested over time to track severity of each of one or more chromosomal regions and a number of chromosomal regions to enable screening and prognosticating, as well as monitoring of progress (e.g. after treatment).

    LABELED NUCLEOTIDE ANALOGS, REACTION MIXTURES, AND METHODS AND SYSTEMS FOR SEQUENCING
    28.
    发明申请
    LABELED NUCLEOTIDE ANALOGS, REACTION MIXTURES, AND METHODS AND SYSTEMS FOR SEQUENCING 有权

    公开(公告)号：US20250002989A1

    公开(公告)日：2025-01-02

    申请号：US18675708

    申请日：2024-05-28

    Applicant: Pacific Biosciences of California, Inc.

    Inventor： Lubomir SEBO ,  Gene SHEN ,  Stephen YUE ,  Honey OSUNA ,  Yuri LAPIN ,  Louis BROGLEY ,  Andrei FEDOROV

    IPC: C12Q1/6869 ,  C07H19/10 ,  C07H19/207 ,  C07K19/00 ,  C09B69/10

    Abstract: Labeled nucleotide analogs comprising at least one avidin protein, at least one dye-labeled compound, and at least one nucleotide compound are provided. The analogs are useful in various fluorescence-based analytical methods, including the analysis of highly multiplexed optical reactions in large numbers at high densities, such as single molecule real time nucleic acid sequencing reactions. The analogs are detectable with high sensitivity at desirable wavelengths. They contain structural components that modulate the interactions of the analogs with DNA polymerase, thus decreasing photodamage and improving the kinetic and other properties of the analogs in sequencing reactions. Also provided are nucleotide and dye-labeled compounds of the subject analogs, as well as intermediates useful in the preparation of the compounds and analogs. Compositions comprising the compounds, methods of synthesis of the intermediates, compounds, and analogs, and mutant DNA polymerases are also provided.

    Methods and apparatus that increase sequencing-by-binding efficiency
    29.
    发明授权
    Methods and apparatus that increase sequencing-by-binding efficiency 有权

    公开(公告)号：US12180545B2

    公开(公告)日：2024-12-31

    申请号：US17876893

    申请日：2022-07-29

    Applicant: Pacific Biosciences of California, Inc.

    Inventor： Sean Stromberg ,  John Vieceli ,  Kandaswamy Vijayan ,  Arnold Oliphant

    IPC: C12Q1/68 ,  C12Q1/6832 ,  C12Q1/6853 ,  C12Q1/6869 ,  C12Q1/6874

    Abstract: A method of determining a nucleic acid sequence that includes steps of: (a) contacting a primed template nucleic acid with a series of mixtures for forming ternary complexes, wherein each of the mixtures includes a polymerase and nucleotide cognates for at least two different base types suspected of being present at the next template position of the template nucleic acid; (b) monitoring the next template position for ternary complexes formed by the series of mixtures, wherein a signal state indicates presence or absence of ternary complex formed at the next template position by each individual mixture, thereby determining a series of signal states that encodes a base call for the next template position; and (c) decoding the series of signal states to distinguish a correct base call for the next template position from an error in the base call.

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