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    Methods and compositions for detecting larval taenia solium with a cloned diagnostic antigen
    22.
    发明申请
    Methods and compositions for detecting larval taenia solium with a cloned diagnostic antigen 有权
    用克隆的诊断抗原检测幼虫绦虫的方法和组合物

    公开(公告)号：US20040033540A1

    公开(公告)日：2004-02-19

    申请号：US10240982

    申请日：2003-02-20

    发明人： Victor C .W. Tsang ,  Ryan M. Greene ,  Patricia P. Wilkins ,  Kathy Hancock

    IPC分类号： G01N033/53 ,  G01N033/569 ,  C07K016/20

    CPC分类号： G01N33/56905 ,  C07K14/43554 ,  C07K16/18 ,  C12Q1/6888 ,  G01N33/5308 ,  Y10S435/805

    摘要： Compositions and methods for the detection of Taenia solium and the diagnosis of T. solium infection are described. The nucleotide and amino acid sequences of the antigenic T. solium polypeptides gp50a, gp50b and gp50c are provided. The compositions contain synthetic antigenic polypeptides of larval origin prepared using the sequences described herein. Probes and primers for the detection or amplification of T. solium nucleic acid molecules are also described. The polypeptides can be administered to a human or animal to protect against T. solium infection. In addition, the polypeptides are useful as research tools for studying T. solium and as reagents in assays for the detection of T. solium antibodies in a biological sample. The methods are sensitive and specific assays that utilize the stable recombinant or synthetic antigenic polypeptides or nucleic acid molecules encoding the larval polypeptides.

    摘要翻译： 描述了用于检测绦虫的组合物和方法以及T.solium感染的诊断。 提供抗原性T.solium多肽gp50a，gp50b和gp50c的核苷酸和氨基酸序列。 组合物含有使用本文所述的序列制备的幼虫来源的合成抗原多肽。 还描述了用于检测或扩增T.solium核酸分子的探针和引物。 多肽可以施用于人或动物以防止T.solium感染。 此外，该多肽可用作研究T.solium的研究工具，并且作为用于检测生物样品中T.solium抗体的测定的试剂。 该方法是利用稳定的重组或合成抗原多肽或编码幼虫多肽的核酸分子的敏感和特异性测定法。

    Bactericidal/permeability-increasing protein: crystallization, x-ray diffraction, three-dimensional structure determination, rational drug design and molecular modeling of related proteins
    23.
    发明申请
    Bactericidal/permeability-increasing protein: crystallization, x-ray diffraction, three-dimensional structure determination, rational drug design and molecular modeling of related proteins 审中-公开
    杀菌/渗透性增加的蛋白质：结晶，x射线衍射，三维结构测定，相关蛋白质的合理药物设计和分子模拟

    公开(公告)号：US20040014153A1

    公开(公告)日：2004-01-22

    申请号：US10162743

    申请日：2002-06-05

    发明人： Lesa J. Beamer ,  Stephen F. Carroll ,  David Eisenberg ,  Gary Kleiger

    IPC分类号： G01N033/554 ,  G01N033/569 ,  G06F019/00 ,  G01N033/48 ,  G01N033/50

    CPC分类号： C07K14/4742 ,  C07K1/306 ,  G16B5/00 ,  G16C10/00 ,  G16C20/50 ,  Y02A90/26

    摘要： The present invention solves the three-dimensional structure of BPI and thereby provides atomic coordinates of BPI from the analysis of x-ray diffraction patterns of sufficiently high resolution for three-dimensional structure determination of the protein, as well as methods for rational drug design, based on using amino acid sequence data and/or x-ray diffraction data provided on computer readable media, as analyzed on a computer system having suitable computer algorithms; and atomic coordinates are provided yielding structural information on related proteins, including the lipid binding and lipid transport protein family that includes BPI, LBP, CETP and PLTP.

    摘要翻译： 本发明解决了BPI的三维结构，从而提供了足够高分辨率的x射线衍射图谱的分析用于蛋白质的三维结构测定的BPI的原子坐标，以及合理的药物设计方法， 基于在具有合适的计算机算法的计算机系统上分析的使用在计算机可读介质上提供的氨基酸序列数据和/或x射线衍射数据; 并提供原子坐标，产生相关蛋白质的结构信息，包括脂质结合和脂质转运蛋白家族，包括BPI，LBP，CETP和PLTP。

    Apparatus and method for detecting bacteria in blood products
    25.
    发明申请
    Apparatus and method for detecting bacteria in blood products 审中-公开
    血液制品中细菌检测装置及方法

    公开(公告)号：US20040009542A1

    公开(公告)日：2004-01-15

    申请号：US10428513

    申请日：2003-05-02

    申请人： Gambro, Inc.

    发明人： Larry Joe Dumont ,  Marlene Adele Bainbridge ,  Tay Goodrich ,  Keith Manica

    IPC分类号： G01N033/554 ,  G01N033/569 ,  A61M005/178

    CPC分类号： A61J1/10 ,  A61J1/12 ,  A61M1/0236 ,  A61M1/3496 ,  A61M2202/0225 ,  A61M2205/123 ,  A61M2205/125 ,  A61M2205/128

    摘要： An apparatus and method for penetrating a bacteria detection culture bottle in an aseptic manner. The apparatus includes the use of a sample bulb pre-connected to a product bag along with a sampler coupler including a needle or hollow spike. The apparatus also includes for the use of a sample bag or sample bulb and penetrating needle or hollow spike pre-connected or sterilely connected to a product container so that a sample may be taken for bacteria detection without contaminating the product in the product container.

    摘要翻译： 用于以无菌方式穿透细菌检测培养瓶的装置和方法。 该设备包括使用预先连接到产品袋的样品灯泡以及包括针或中空尖峰的采样器耦合器。 该设备还包括使用样品袋或样品灯泡以及预先连接或无菌地连接到产品容器的穿刺针或中空尖钉，使得可以将样品用于细菌检测而不污染产品容器中的产品。

    Epitope tag recognized by a monoclonal antibody to Rickettsia typhi
    30.
    发明申请
    Epitope tag recognized by a monoclonal antibody to Rickettsia typhi 审中-公开
    表达标签被单克隆抗体识别为立克次体感染

    公开(公告)号：US20030186340A1

    公开(公告)日：2003-10-02

    申请号：US10199263

    申请日：2002-07-18

    发明人： Myong-Joon Hahn

    IPC分类号： G01N033/554 ,  G01N033/569

    CPC分类号： G01N33/56911 ,  A61K39/00 ,  C07K14/29 ,  C07K16/1246 ,  C07K2317/34 ,  Y02A50/403 ,  Y02A50/59

    摘要： The epitope recognized by a mouse monoclonal antibody (MAb) to the crystalline surface layer protein (SLP) of Rickettsia typhi, SRT10, is mapped to ten amino acid residues (SRTag, TFIGAIATDT). The oligonucleotide sequence covering the epitope recognized by SRT10 is inserted to a mammalian expression vector together with multiple cloning sites. When the SRTag is fused in frame to the coding region of protein gene and expressed in bacteria or mammalian cells, the MAb SRT10 can detect the tagged protein by immunoblotting, immunocytochemistry, and immunoprecipitation.

    摘要翻译： 由鼠单克隆抗体（MAb）识别的表达克隆立克次氏体SRT10的结晶表面蛋白（SLP）的表位被映射到10个氨基酸残基（SRTag，TFIGAIATDT）。 将覆盖SRT10识别的表位的寡核苷酸序列与多个克隆位点一起插入到哺乳动物表达载体中。 当SRTag与蛋白质基因的编码区融合并在细菌或哺乳动物细胞中表达时，MAb SRT10可以通过免疫印迹，免疫细胞化学和免疫沉淀检测标记的蛋白质。