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31.发明申请公开(公告)号：US20220379320A1
公开(公告)日：2022-12-01
申请号：US17654938
申请日：2022-03-15
Applicant: Berkeley Lights, Inc.
Inventor： Kevin T. Chapman , Eric D. Hobbs , Steven W. Short , Mark P. White , Daniele Malleo
Abstract: Individual biological cells can be selected in a micro-fluidic device and moved into isolation pens in the device. The cells can then be lysed in the pens, releasing nucleic acid material, which can be captured by one or more capture objects in the pens. The capture objects with the captured nucleic acid material can then be removed from the pens. The capture objects can include unique identifiers, allowing each capture object to be correlated to the individual cell from which the nucleic acid material captured by the object originated.
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32.发明授权公开(公告)号：US11318479B2
公开(公告)日：2022-05-03
申请号：US15105849
申请日：2014-12-18
Applicant: Berkeley Lights, Inc.
Inventor： Kevin T. Chapman , Eric D. Hobbs , Steven W. Short , Mark P. White , Daniele Malleo
Abstract: Individual biological cells can be selected in a micro-fluidic device and moved into isolation pens in the device. The cells can then be lysed in the pens, releasing nucleic acid material, which can be captured by one or more capture objects in the pens. The capture objects with the captured nucleic acid material can then be removed from the pens. The capture objects can include unique identifiers, allowing each capture object to be correlated to the individual cell from which the nucleic acid material captured by the object originated.
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公开(公告)号：US11007520B2
公开(公告)日：2021-05-18
申请号：US16196649
申请日：2018-11-20
Applicant: Berkeley Lights, Inc.
Inventor： Randall D. Lowe, Jr. , Alexander J. Mastroianni , Mark P. White , Gregory G. Lavieu , Kristin G. Beaumont
IPC: B01L3/00 , C12M3/06 , B81C1/00 , G01N33/543
Abstract: In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects. Described herein are surface modifying and surface functionalizing reagents, preparation thereof, and methods for modifying surfaces to provide improved or altered performance with biomaterials.
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公开(公告)号：US10723988B2
公开(公告)日：2020-07-28
申请号：US15135707
申请日：2016-04-22
Applicant: Berkeley Lights, Inc.
Inventor： Randall D. Lowe, Jr. , Kristin Beaumont , Aathavan Karunakaran , Natalie Marks , Jason M. McEwen , Mark P. White , J. Tanner Nevill , Gang F. Wang , Andrew W. McFarland , Daniele Malleo , Keith J. Breinlinger , Xiao Guan , Kevin T. Chapman
Abstract: Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.
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35.发明授权公开(公告)号：US10712344B2
公开(公告)日：2020-07-14
申请号：US15406289
申请日：2017-01-13
Applicant: Berkeley Lights, Inc.
Inventor： Kevin T. Chapman , George L. Fox , Peggy A. Radel , Mark P. White , Xiaohua Wang , Minha Park , Guido K. Stadler , Randall D. Lowe, Jr. , Xiao Guan Radstrom , Jason M. McEwen , Gang F. Wang
IPC: B01L3/00 , G01N33/574 , C07K16/00 , G01N33/569 , C07K16/28 , G01N33/50 , C07K16/30 , C12Q1/6886 , G01N33/543
Abstract: A method of preparing an antibody therapeutic is provided comprising: (a) providing a dissociated cell sample from at least one solid tumor sample obtained from a patient; (b) loading the dissociated cell sample into a microfluidic device having a flow region and at least one isolation region fluidically connected to the flow region; (c) moving at least one B cell from the dissociated cell sample into at least one isolation region in the microfluidic device, thereby obtaining at least one isolated B cell; and (d) using the microfluidic device to identify at least one B cell that produces antibodies capable of binding to cancer cells. The cancer cells can be the patient's own cancer cells. Also provided are methods of treating patients, methods of labeling or detecting cancer, engineered T or NK cells comprising antibodies or fragments thereof, and engineered antibody constructs.
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Patent Agency Ranking
公开(公告)号：US10101250B2
公开(公告)日：2018-10-16
申请号：US15136763
申请日：2016-04-22
Applicant: Berkeley Lights, Inc.
Inventor： Mark P. White , Randall D. Lowe, Jr.
IPC: G01N1/34 , B01L3/00 , B03C5/00 , B03C5/02 , C12Q1/6806 , G01N27/447
Abstract: Aspects of the present disclosure are directed to the manipulation of a cell nucleus in a micro-fluidic device as well as compositions, systems, and kits for performing such methods. In some aspects, the disclosure provides methods for placing one or more selected cell nuclei into an isolation region of a sequestration pen in a micro-fluidic device. The isolated nucleus/nuclei may then be retrieved from the isolation region of the sequestration pen and used in any desired downstream assay or process.
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公开(公告)号：US20180272350A1
公开(公告)日：2018-09-27
申请号：US15843122
申请日：2017-12-15
Applicant: Berkeley Lights, Inc.
Inventor： Kevin T. Chapman , Daniele Malleo , J. Tanner Nevill , Steven W. Short , Mark P. White , M. Jimena Loureiro
CPC classification number: B01L3/502761 , B01L2200/0647 , B01L2200/0668 , B01L2300/0636 , B01L2300/0816 , B01L2300/0864 , B01L2300/087 , B01L2400/0454 , B03C5/005 , B03C5/026 , B03C2201/26 , G01N15/1484 , G01N33/5023 , G01N33/5047 , G01N33/505 , G01N33/5052 , G01N33/54313 , G01N33/6854
Abstract: Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.
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公开(公告)号：US20180099282A1
公开(公告)日：2018-04-12
申请号：US15659855
申请日：2017-07-26
Applicant: Berkeley Lights, Inc.
Inventor： Keith J. Breinlinger , Eric D. Hobbs , Daniele Malleo , J. Tanner Nevill , Mark P. White
CPC classification number: B01L3/502761 , B01L3/502715 , B01L3/50273 , B01L2200/0668 , B01L2300/0816 , B01L2300/0819 , B01L2300/0848 , B01L2300/0877 , B01L2400/0424 , B01L2400/0427 , B01L2400/0454 , B01L2400/0457 , B01L2400/086 , G01N1/4077 , G01N2001/4083
Abstract: A microfluidic apparatus is provided having one or more sequestration pens configured to isolate one or more target micro-objects by changing the orientation of the microfluidic apparatus with respect to a globally active force, such as gravity. Methods of selectively directing the movements of micro-objects in such a microfluidic apparatus using gravitational forces are also provided. The micro-objects can be biological micro-objects, such as cells, or inanimate micro-objects, such as beads.
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公开(公告)号：US20160312165A1
公开(公告)日：2016-10-27
申请号：US15135707
申请日：2016-04-22
Applicant: Berkeley Lights, Inc.
Inventor： Randall D. Lowe, JR. , Kristin Beaumont , Aathavan Karunakaran , Natalie Marks , Jason M. McEwen , Mark P. White , J. Tanner Nevill , Gang F. Wang , Andrew W. McFarland , Daniele Malleo , Keith J. Breinlinger , Xiao Guan , Kevin T. Chapman
Abstract: Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.
Abstract translation: 描述了用于在微流体装置中培养一个或多个生物细胞的系统，方法和试剂盒，包括提供配置成增强细胞生长，活力，可移植性或其任何组合的营养物和气体组分。 在一些实施方案中，培养单细胞可以在微流体装置中产生克隆群体。
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公开(公告)号：US11998914B2
公开(公告)日：2024-06-04
申请号：US17656244
申请日：2022-03-24
Applicant: Berkeley Lights, Inc.
Inventor： Kevin T. Chapman , Daniele Malleo , J. Tanner Nevill , Steven W. Short , Mark P. White , M. Jimena Loureiro
CPC classification number: B01L3/502761 , B03C5/005 , B03C5/026 , G01N15/1484 , G01N33/5023 , G01N33/5047 , G01N33/505 , G01N33/5052 , G01N33/54313 , G01N33/6854 , B01L2200/0647 , B01L2200/0668 , B01L2300/0636 , B01L2300/0816 , B01L2300/0864 , B01L2300/087 , B01L2400/0454 , B03C2201/26
Abstract: Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.
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