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公开(公告)号：US07329525B2
公开(公告)日：2008-02-12
申请号：US11014051
申请日：2004-12-16
申请人： David A. Estell
发明人： David A. Estell
摘要： The present invention relates to the identification of novel serine proteases in Gram-positive microorganisms. The present invention provides the nuclei acid and amino acid sequences for the Bacillus subtilis serine proteases SP1, SP2, SP3, SP4 and SP5. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding SP1, SP2, SP3, SP4 and SP5. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides a cleaning composition comprising a serine protease of the present invention.
摘要翻译： 本发明涉及革兰氏阳性微生物中新型丝氨酸蛋白酶的鉴定。 本发明提供枯草芽孢杆菌丝氨酸蛋白酶SP1，SP2，SP3，SP4和SP5的核酸和氨基酸序列。 本发明还提供具有编码SP1，SP2，SP3，SP4和SP5的基因的部分或全部的突变或缺失的宿主细胞。 本发明还提供宿主细胞，其进一步包含编码所需异源蛋白质如酶的核酸。 本发明还提供了包含本发明的丝氨酸蛋白酶的清洁组合物。
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公开(公告)号：US06794179B2
公开(公告)日：2004-09-21
申请号：US10188586
申请日：2002-07-02
申请人： David A. Estell
发明人： David A. Estell
IPC分类号： C12N1511
CPC分类号： C12N9/54 , A23K20/189 , C11D3/386 , D06M16/003
摘要： The present invention relates to the identification of a novel metalloprotease in gram positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for the metalloprotease. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding the metalloprotease. The present invention provides host cells which further comprises a nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions, animal feeds and compositions used to treat a textile that include the metalloprotease of the present invention.
摘要翻译： 本发明涉及革兰氏阳性微生物中新型金属蛋白酶的鉴定。 本发明提供了金属蛋白酶的核酸和氨基酸序列。 本发明还提供具有编码金属蛋白酶的基因的部分或全部的突变或缺失的宿主细胞。 本发明提供宿主细胞，其进一步包含编码所需异源蛋白质如酶的核酸。 本发明还提供了用于处理包括本发明的金属蛋白酶的织物的清洁组合物，动物饲料和组合物。
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公开(公告)号：US06723550B1
公开(公告)日：2004-04-20
申请号：US09462845
申请日：2000-01-13
申请人： David A. Estell
发明人： David A. Estell
IPC分类号： C12N948
摘要： The present invention relates to the identification of novel serine proteases in Gram-positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for the Bacillus subtilis serine proteases SP1, SP2, SP3, SP4 and SP5. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding SP1, SP2, SP3, SP4 and SP5. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides a cleaning composition comprising a serine protease of the present invention.
摘要翻译： 本发明涉及革兰氏阳性微生物中新型丝氨酸蛋白酶的鉴定。 本发明提供枯草芽孢杆菌丝氨酸蛋白酶SP1，SP2，SP3，SP4和SP5的核酸和氨基酸序列。 本发明还提供具有编码SP1，SP2，SP3，SP4和SP5的基因的部分或全部的突变或缺失的宿主细胞。 本发明还提供宿主细胞，其进一步包含编码所需异源蛋白质如酶的核酸。 本发明还提供了包含本发明的丝氨酸蛋白酶的清洁组合物。
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公开(公告)号：US06300117B1
公开(公告)日：2001-10-09
申请号：US09308375
申请日：1999-05-14
申请人： David A. Estell
发明人： David A. Estell
IPC分类号： C12N954
CPC分类号： C12N9/54 , A23K20/189 , C11D3/386 , C12N15/75 , D06M16/003
摘要： The present invention relates to the identification of novel metallo-proteases (MP) in Gram-positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for Bacillus (MP). The present invention also provides host cells having mutation or deletion of part or all of the gene encoding MP. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions comprising an MP of the present invention.
摘要翻译： 本发明涉及革兰阳性微生物中新型金属蛋白酶（MP）的鉴定。 本发明提供芽孢杆菌（MP）的核酸和氨基酸序列。 本发明还提供具有编码MP的基因的部分或全部的突变或缺失的宿主细胞。 本发明还提供宿主细胞，其进一步包含编码所需异源蛋白质如酶的核酸。 本发明还提供了包含本发明的MP的清洁组合物。
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公开(公告)号：US5185258A
公开(公告)日：1993-02-09
申请号：US600430
申请日：1990-10-19
IPC分类号： C07K14/195 , C07K14/00 , C07K14/32 , C07K14/41 , C11D20060101 , C11D3/386 , C11D10/00 , C12N1/21 , C12N9/00 , C12N9/14 , C12N9/16 , C12N9/20 , C12N9/50 , C12N9/54 , C12N9/56 , C12N15/00 , C12N15/09 , C12N15/10 , C12N15/57 , C12N15/75 , C12P7/64 , C12P21/02
CPC分类号： C11D3/38609 , C11D3/386 , C12N15/102 , C12N15/75 , C12N9/14 , C12N9/16 , C12N9/20 , C12N9/50 , C12N9/54 , C12P7/6418 , C12R1/07
摘要： Novel carbonyl hydrolase mutants derived from the DNA sequences of naturally-occurring or recombinant non-human carbonyl hydrolases are disclosed. The mutant carbonyl hydrolases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant carbonyl hydrolase to generate the substitution of one or more amino acid residues in the amino acid sequence of a precursor carbonyl hydrolase. Such mutant carbonyl hydrolases have properties which are different from those of the precursor hydrolase and are especially useful in detergent formulations. The substituted amino acid residues correspond to position +123 and/or +274 in Bacillus amyloliquefaciens subtilisin.
摘要翻译： 公开了衍生自天然存在或重组非人羰基水解酶的DNA序列的新型羰基水解酶突变体。 突变体羰基水解酶通常通过体外修饰编码天然存在或重组羰基水解酶的前体DNA序列获得，以产生前体羰基水解酶的氨基酸序列中一个或多个氨基酸残基的取代。 这种突变型羰基水解酶具有与前体水解酶不同的性质，并且在洗涤剂配方中特别有用。 取代的氨基酸残基对应于解淀粉芽孢杆菌枯草杆菌蛋白酶中的位置+123和/或+ 274。
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36.发明授权Subtilisins modified at position 225 resulting in a shift in catalytic activity 失效在225位修饰的枯草杆菌蛋白酶导致催化活性的变化
公开(公告)号：US5155033A
公开(公告)日：1992-10-13
申请号：US294340
申请日：1989-01-06
IPC分类号： C12N9/56
CPC分类号： C12N9/54
摘要： There are described certain subtilisins wherein the amino acid sequence of such subtilisins has been modified at a position equivalent to +225 in Bacillus amyloliquefaciens, such that an amino acid selected from the group consisting of alanine, leucine, methionine, glutamine, valine, and serine, has been substituted for the amino acid residue naturally occurring at such position.
摘要翻译： 描述了某些枯草杆菌蛋白酶，其中这种枯草杆菌蛋白酶的氨基酸序列已经在解淀粉芽孢杆菌中相当于+225的位置被修饰，使得选自丙氨酸，亮氨酸，甲硫氨酸，谷氨酰胺，缬氨酸和丝氨酸的氨基酸 已取代天然存在于该位置的氨基酸残基。
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公开(公告)号：US09273298B2
公开(公告)日：2016-03-01
申请号：US13283564
申请日：2011-10-27
IPC分类号： C12N9/88 , C12N1/20 , C12N15/00 , C12N1/14 , C07H21/04 , C12N15/79 , C12N15/74 , C12N15/63 , C12P5/00
摘要： The present invention provides methods and compositions comprising at least one isoprene synthase enzyme with improved specific productivity. In particular, the present invention provides variant plant isoprene synthases for increased isoprene production in host cells.
摘要翻译： 本发明提供包含至少一种具有提高的比生产率的异戊二烯合酶的方法和组合物。 特别地，本发明提供用于增加宿主细胞中异戊二烯生产的变体植物异戊二烯合成酶。
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公开(公告)号：US09133425B2
公开(公告)日：2015-09-15
申请号：US12602991
申请日：2008-06-06
IPC分类号： C40B30/10 , C11D3/02 , C11D3/37 , C07K1/00 , C07H21/04 , C12N9/14 , C12N9/16 , C12N9/24 , C12N9/26 , C11D3/386 , C12N9/28 , C12N9/54 , C12N15/10
CPC分类号： C12N9/54 , C11D3/386 , C11D3/38681 , C12N9/2411 , C12N9/2417 , C12N15/1034 , C12Y302/01001 , C12Y304/21062
摘要： The present invention provides efficient methods for obtaining a protein with one or more beneficial attributes in industrial, consumer or pharmaceutical applications. In some preferred embodiments, the present invention provides methods for producing superior enzymes for a given application through screening an abbreviated set of candidate enzymes.
摘要翻译： 本发明提供了在工业，消费者或医药应用中获得具有一个或多个有益属性的蛋白质的有效方法。 在一些优选的实施方案中，本发明提供了通过筛选候选酶的缩写产生用于给定应用的优良酶的方法。
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39.发明授权Compositions and methods comprising alpha-amylase variants with altered properties 有权包含具有改变性质的α-淀粉酶变体的组合物和方法公开(公告)号：US08852912B2
公开(公告)日：2014-10-07
申请号：US13260146
申请日：2010-04-01
IPC分类号： C12P21/06 , C12N9/00 , C12N9/28 , C07K1/00 , C07H21/04 , G01N33/48 , G01N31/00 , C11D3/386 , C12N9/54
CPC分类号： C11D3/386 , C11D3/38681 , C12N9/2417 , C12N9/54
摘要： Described are compositions and methods relating to variant alpha-amylases having altered biochemical properties and advantageous performance characteristics as compared to a reference alpha-amylase. The variants are suitable for use in various industrial applications such as starch conversion, ethanol production, laundry, dishwashing, pulp and paper production, textile desizing, and/or sweetener production.
摘要翻译： 描述了与参考α-淀粉酶相比具有改变的生物化学性质和有利的性能特征的变体α-淀粉酶相关的组合物和方法。 这些变体适用于各种工业应用，如淀粉转化，乙醇生产，洗衣，洗碗，纸浆和造纸，纺织品退浆和/或甜味剂生产。
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公开(公告)号：US08753861B2
公开(公告)日：2014-06-17
申请号：US13128854
申请日：2009-11-10
CPC分类号： C12N9/54 , C11D3/386 , C11D3/38609 , C11D3/38618 , C11D3/38663 , C11D3/38681 , C12N15/52 , C12Y304/21062
摘要： The present invention provides engineered protease variants. In particular, the protease variants comprise combinable mutations at selected surface positions that affect the charge and/or hydrophobicity of the enzyme to enhance at least one desired property of the resulting variant enzyme in a chosen application. Compositions comprising the protease variants, and methods for using the same are also provided.
摘要翻译： 本发明提供工程蛋白酶变体。 特别地，蛋白酶变体在选定的表面位置包含影响酶的电荷和/或疏水性的可组合突变，以在所选择的应用中增强所得变体酶的至少一种所需性质。 还提供了包含蛋白酶变体的组合物及其使用方法。
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