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公开(公告)号：US20140055853A1
公开(公告)日：2014-02-27
申请号：US13595175
申请日：2012-08-27
CPC分类号： G01N1/31 , B01L3/502715 , B01L3/508 , B01L9/52 , B01L9/527 , B01L2300/041 , B01L2300/046 , B01L2300/0822 , B01L2300/0877 , G01N1/312 , G01N2001/317
摘要： An open top microfluidic device comprising a microfluidic slide carrier and one or more multiplexing stations is provided which allows sequential staining and imaging without the need for using or removing a coverslip on a mounted biological sample.
摘要翻译： 提供了包括微流体载玻片载体和一个或多个多路复用站的开口顶部微流体装置，其允许顺序染色和成像，而不需要在安装的生物样品上使用或去除盖玻片。
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32.发明申请QUANTITATIVE PHASE MICROSCOPY FOR HIGH-CONTRAST CELL IMAGING USING FREQUENCY DOMAIN PHASE SHIFT 有权使用频域相移的高对比度细胞成像的定量相位显微镜公开(公告)号：US20130335548A1
公开(公告)日：2013-12-19
申请号：US13523816
申请日：2012-06-14
IPC分类号： H04N7/18
CPC分类号： G02B21/14 , G01B9/04 , G01B2290/20 , G02B21/0088 , G02B21/361
摘要： Some systems described herein include a frequency dependent phase plate for generating multiple phase-contrast images of a sample, each from a different frequency range of light, each phase-contrast image for frequency range of light formed from light diffracted by the sample interfered with undiffracted light that has a frequency-dependent baseline relative phase shift from the phase plate. In some embodiments, the multiple phase-contrast images may be used to generate a quantitative phase image of a sample. The phase-contrast images or the produced quantitative phase image may have sufficient contrast for label-free auto-segmentation of cell bodies and nuclei.
摘要翻译： 本文描述的一些系统包括频率相关相位板，用于产生每个来自不同频率范围的样品的多个相位对比图像，用于由被样品干涉的未衍射的光形成的光的频率范围的每个相位对比度图像 具有频率相关基准的相对于相位板相对相移的光。 在一些实施例中，多个相位对比图像可用于产生样本的定量相位图像。 相位对比图像或产生的定量相位图像可以具有足够的对比度，用于无标记的细胞体和核的自动分割。
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公开(公告)号：US08532398B2
公开(公告)日：2013-09-10
申请号：US12732272
申请日：2010-03-26
CPC分类号： G02B21/16 , G01N21/6458 , G02B21/06 , G02B21/365
摘要： A method for the imaging of protein expression and location in biological samples using optical segmentation is provided. The steps comprise acquiring a fluorescent image of a biological sample, analyzing the image and generating a masking pattern corresponding to a specific structure within the biological sample, transforming the masking pattern into the spatial coordinates of a digital micro-mirror device (DMD) which may then be projected onto the biological sample and obtaining a masked fluorescent image. Also provided is an image analysis system for imaging of protein expression and location in biological samples using optical segmentation.
摘要翻译： 提供了一种使用光学分割技术在生物样品中成像蛋白质表达和定位的方法。 步骤包括获取生物样品的荧光图像，分析图像并产生对应于生物样品内的特定结构的掩模图案，将掩模图案变换成数字微镜装置（DMD）的空间坐标，其可以 然后投射到生物样品上并获得掩蔽的荧光图像。 还提供了一种用于使用光学分割对生物样品中蛋白质表达和位置成像进行成像的图像分析系统。
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34.发明申请公开(公告)号：US20130163002A1
公开(公告)日：2013-06-27
申请号：US13335748
申请日：2011-12-22
IPC分类号： G01B9/02
CPC分类号： G02B21/367 , G02B21/14
摘要： Systems and methods described herein employ multiple phase-contrast images with various relative phase shifts between light diffracted by a sample and light not diffracted by the sample to produce a quantitative phase image. The produced quantitative phase image may have sufficient contrast for label-free auto-segmentation of cell bodies and nuclei.
摘要翻译： 本文描述的系统和方法使用多个相差图像，其在由样品衍射的光和不被样品衍射的光之间具有各种相对相移，以产生定量相位图像。 所产生的定量相图可能具有足够的对比度，用于细胞体和细胞核的无标记自动分割。
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公开(公告)号：US20120134603A1
公开(公告)日：2012-05-31
申请号：US12956909
申请日：2010-11-30
申请人： Zhengyu Pang , Robert John Filkins
发明人： Zhengyu Pang , Robert John Filkins
IPC分类号： G06K9/32
CPC分类号： G06T5/009 , G06T2207/10064 , G06T2207/30024
摘要： Methods for scaling an image taken an optimal exposure time to a selected exposure time, generally comprising, determining a dark pixel intensity of an imaging device; acquiring a first image at an optimal exposure time; and adjusting a pixel intensity of one or more pixels in the first image, based at least in part on the dark pixel intensity, for a second exposure time that is different from the optimal exposure time.
摘要翻译： 用于将拍摄了最佳曝光时间的图像缩放到所选择的曝光时间的方法，通常包括：确定成像装置的暗像素强度; 以最佳曝光时间获取第一图像; 以及至少部分地基于所述暗像素强度来调整与所述最佳曝光时间不同的第二曝光时间的所述第一图像中的一个或多个像素的像素强度。
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公开(公告)号：US07741045B2
公开(公告)日：2010-06-22
申请号：US11864085
申请日：2007-09-28
申请人： Michael J. Gerdes , Anup Sood , Michael Christopher Montalto , Ali Can , Fiona Ginty , Maureen Ann Bresnahan , Robert John Filkins , Zhengyu Pang
发明人： Michael J. Gerdes , Anup Sood , Michael Christopher Montalto , Ali Can , Fiona Ginty , Maureen Ann Bresnahan , Robert John Filkins , Zhengyu Pang
CPC分类号： G01N33/582 , C12Q1/6816 , G01N33/542 , C12Q2545/101 , C12Q2527/125
摘要： Methods for probing multiple targets in a biological sample are provided. The methods include the steps of providing a biological sample containing multiple targets, binding at least one fluorescent probe to one or more target present in the sample, and binding at least at least one control probe to one or more target present in the sample. The methods include the steps of observing a signal from the fluorescent probe and a control signal from the control probe and applying to the sample a basic solution containing an oxidizing agent that selectively inactivates the fluorescent probe and not the control probe. The methods further include the steps of binding at least one fluorescent probe to one or more target present in the sample and observing a signal from the fluorescent probe. The methods disclosed herein also provide for multiple iterations of binding, observing, and oxidizing for deriving information about multiple targets in a single sample. An associated kit is also provided.
摘要翻译： 提供了探测生物样品中多个靶标的方法。 所述方法包括以下步骤：提供含有多个靶标的生物样品，将至少一种荧光探针结合至样品中存在的一种或多种靶标，以及将至少一种对照探针结合至样品中存在的一种或多种靶标。 所述方法包括以下步骤：观察来自荧光探针的信号和来自对照探针的控制信号，并向样品施加含有选择性灭活荧光探针而不是对照探针的氧化剂的碱性溶液。 所述方法还包括将至少一种荧光探针结合到样品中存在的一种或多种目标物并观察来自荧光探针的信号的步骤。 本文公开的方法还提供结合，观察和氧化的多次迭代，用于导出关于单个样本中的多个目标的信息。 还提供了相关的套件。
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公开(公告)号：US20100047925A1
公开(公告)日：2010-02-25
申请号：US12611299
申请日：2009-11-03
申请人： Anup Sood , Michael Christopher Montalto , Michael Gerdes , Maximilian Lewis Seel , Robert John Filkins
发明人： Anup Sood , Michael Christopher Montalto , Michael Gerdes , Maximilian Lewis Seel , Robert John Filkins
IPC分类号： G01N33/53
CPC分类号： G01N33/53 , C12Q1/68 , G01N33/542 , G01N33/582 , Y10T436/143333
摘要： Methods for detecting multiple targets in a biological sample are provided. The methods includes contacting the sample with a first probe; physically binding the first probe to a first target; observing a first signal from the first probe; applying a chemical agent to modify the first signal; contacting the sample with a second probe; physically binding the second probe to a second target; and observing a second signal from the second probe. The methods disclosed herein also provide for multiple iterations of binding, observing, signal modification for deriving information about multiple targets in a single sample. An associated kit and device are also provided.
摘要翻译： 提供了用于检测生物样品中多个靶的方法。 所述方法包括使样品与第一探针接触; 将第一探针物理地绑定到第一靶; 观察来自第一探针的第一信号; 施用化学试剂来修饰第一信号; 使样品与第二探针接触; 将第二探针物理地绑定到第二靶上; 并观察来自第二探测器的第二信号。 本文公开的方法还提供绑定，观察，信号修改的多次迭代，用于导出关于单个样本中的多个目标的信息。 还提供了相关的套件和设备。
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公开(公告)号：US20090253163A1
公开(公告)日：2009-10-08
申请号：US12061044
申请日：2008-04-02
申请人： Jun Xie , Fiona Ginty , Robert John Filkins , Michael Christopher Montalto , Anup Sood , Jeffrey Bernard Fortin , Wei-Cheng Tian , Michael John Gerdes
发明人： Jun Xie , Fiona Ginty , Robert John Filkins , Michael Christopher Montalto , Anup Sood , Jeffrey Bernard Fortin , Wei-Cheng Tian , Michael John Gerdes
CPC分类号： G01N1/312
摘要： Automated methods and devices that facilitate iterative staining of biological samples from imaging applications are provided. The methods include the steps of providing a small volume flow cell containing a biological sample, applying a stain to the biological sample, combining at least two precursor reagents to form an activated destaining agent and wherein the activated destaining agent decomposition rate is greater than or similar to the destaining reaction rate, and flowing the destaining agent over the biological sample at a flow rate that is greater than the decomposition rate of the activated destaining agent. The process of staining, combining and flowing may be iteratively repeated. Also disclosed herein are devices for iterative staining of biological samples comprising a flow cell, in fluid communication with a premixer, wherein the volume capacity of the premixer is smaller than about five times the volume capacity of the flow cell.
摘要翻译： 提供了促进成像应用中生物样品的迭代染色的自动化方法和装置。 所述方法包括以下步骤：提供含有生物样品的小体积流动池，向生物样品施加染色，将至少两种前体试剂结合以形成活化的脱色剂，并且其中所述活化脱色剂分解速率大于或等于 与脱色反应速率相关联，并使脱色剂以大于活化脱色剂分解速率的流速流过生物样品。 染色，组合和流动的过程可以迭代地重复。 本文还公开了用于重复染色生物样品的装置，其包括与预混合器流体连通的流动池，其中预混合器的体积容量小于流动池的体积容量的约五倍。
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公开(公告)号：US20080266652A1
公开(公告)日：2008-10-30
申请号：US11741839
申请日：2007-04-30
CPC分类号： G02B21/245 , G02B21/365
摘要： A digital optical microscope includes a primary image sensor that generates a primary image of a sample at a primary frame rate, an auxiliary image sensor that generates an auxiliary image of the sample at an auxiliary frame rate that is faster than the primary frame rate, and a controller that adjusts a focal distance between an objective lens and the sample along an optical axis in response to the auxiliary image, thereby autofocusing the primary image on the sample. The primary image sensor generates the primary image in response to the autofocusing.
摘要翻译： 数字光学显微镜包括以主要帧速率产生样本的主图像的主图像传感器，以比主帧速率快的辅助帧速率生成样本的辅助图像的辅助图像传感器，以及 控制器，其响应于辅助图像，沿着光轴调整物镜和样本之间的焦距，从而将原始图像自动聚焦在样本上。 主图像传感器响应于自动对焦而产生主图像。
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公开(公告)号：US20080118934A1
公开(公告)日：2008-05-22
申请号：US11864085
申请日：2007-09-28
申请人： Michael J. Gerdes , Anup Sood , Michael Christopher Montalto , Ali Can , Fiona Ginty , Maureen Ann Bresnahan , Robert John Filkins , Zhengyu Pang
发明人： Michael J. Gerdes , Anup Sood , Michael Christopher Montalto , Ali Can , Fiona Ginty , Maureen Ann Bresnahan , Robert John Filkins , Zhengyu Pang
IPC分类号： G01N33/53 , G01N33/566 , G01N33/567
CPC分类号： G01N33/582 , C12Q1/6816 , G01N33/542 , C12Q2545/101 , C12Q2527/125
摘要： Methods for probing multiple targets in a biological sample are provided. The methods include the steps of providing a biological sample containing multiple targets, binding at least one fluorescent probe to one or more target present in the sample, and binding at least at least one control probe to one or more target present in the sample. The methods include the steps of observing a signal from the fluorescent probe and a control signal from the control probe and applying to the sample a basic solution containing an oxidizing agent that selectively inactivates the fluorescent probe and not the control probe. The methods further include the steps of binding at least one fluorescent probe to one or more target present in the sample and observing a signal from the fluorescent probe. The methods disclosed herein also provide for multiple iterations of binding, observing, and oxidizing for deriving information about multiple targets in a single sample. An associated kit is also provided.
摘要翻译： 提供了探测生物样品中多个靶标的方法。 所述方法包括以下步骤：提供含有多个靶标的生物样品，将至少一种荧光探针结合至样品中存在的一种或多种靶标，以及将至少一种对照探针结合至样品中存在的一种或多种靶标。 所述方法包括以下步骤：观察来自荧光探针的信号和来自对照探针的控制信号，并向样品施加含有选择性灭活荧光探针而不是对照探针的氧化剂的碱性溶液。 所述方法还包括将至少一种荧光探针结合到样品中存在的一种或多种目标物并观察来自荧光探针的信号的步骤。 本文公开的方法还提供结合，观察和氧化的多次迭代，用于导出关于单个样本中的多个目标的信息。 还提供了相关的套件。
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