公开(公告)号：US08445265B2

公开(公告)日：2013-05-21

申请号：US11664980

申请日：2005-10-05

申请人： Hideji Tajima ,  Yoshinao Hirahara ,  Osamu Segawa

发明人： Hideji Tajima ,  Yoshinao Hirahara ,  Osamu Segawa

IPC分类号： C12M1/34 ,  G01N21/00

CPC分类号： G01N21/0332 ,  B01L3/0275 ,  B01L3/502 ,  B01L3/5021 ,  B01L3/50851 ,  B01L2200/147 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/0877 ,  B01L2300/1805 ,  B01L2400/0409 ,  B01L2400/0478 ,  G01N21/6428 ,  G01N21/6452 ,  G01N35/0099 ,  G01N2035/00376 ,  G01N2035/00524

摘要： The invention relates to a reaction vessel and a reaction controller, wherein temperature control of a liquid stored within the vessel can be performed with a high accuracy and faithful responsiveness. The reaction container and the reaction controller comprise one or a plurality of reaction chambers in which liquid is storable, and a wall that surrounds the reaction chamber, and the entirety or a part of the wall is formed by a temperature raising and lowering body that can raise or lower the temperature thereof according to a signal from an instructing part provided outside.

公开(公告)号：US08418929B2

公开(公告)日：2013-04-16

申请号：US12450672

申请日：2008-04-07

申请人： Hideji Tajima

发明人： Hideji Tajima

IPC分类号： G05D23/00 ,  G01N31/00 ,  C12M1/00

CPC分类号： B01L7/525 ,  B01L3/021 ,  B01L3/0275 ,  B01L7/52 ,  B01L2200/0684 ,  B01L2200/142 ,  B01L2200/147 ,  B01L2300/0636 ,  B01L2300/0838 ,  B01L2300/18 ,  B01L2300/1822 ,  B01L2300/1844 ,  B01L2300/1888 ,  B01L2400/0478 ,  B01L2400/0487 ,  G01N2035/00346 ,  G01N2035/103 ,  G01N2035/1039 ,  G01N2035/1062 ,  Y10T137/0329 ,  Y10T137/6552

摘要： The present invention relates to a temperature controlling apparatus and the temperature controlling method which can arrest foaming and inflation of the air contained in a liquid, and can perform temperature controlling of a liquid of high reliance, and the present invention is constructed to have one or a plurality of sets of nozzles which can suck and discharge a liquid through a tip, and can retain the sucked liquid, a suction and discharge mechanism which can suck and discharge a gas via the each nozzle, a temperature regulator which can maintain one or two or more set predetermined temperatures for a predetermined time regarding one or two or more predetermined temperature controlling regions set in the each nozzle, a movement mechanism which allows relative movement between one or two or more containers which can accommodate a liquid and a nozzle, and a controlling part which instructs the movement mechanism, the suction and discharge mechanism or the temperature regulator to adjust a liquid to be sucked into the nozzle and a liquid amount thereof, a position of the sucked liquid in the nozzle, and temperature controlling of the liquid.

摘要翻译： 本发明涉及一种温度控制装置和温度控制方法，其可以阻止液体中包含的空气的起泡和膨胀，并且可以执行高依赖性的液体的温度控制，并且本发明构造成具有一个或 多个喷嘴组，可以通过尖端吸取和排出液体，并且可以保持被吸入的液体;吸入和排出机构，其可以通过每个喷嘴吸入和排出气体;温度调节器，其可以保持一个或两个 或更多的预定温度对于设置在每个喷嘴中的一个或两个或更多个预定温度控制区域设定预定温度;允许一个或两个或更多个可容纳液体和喷嘴的容器之间的相对运动的移动机构，以及 控制部分，指示移动机构，吸入和排出机构或温度调节器 仅吸入喷嘴的液体及其液体量，吸入液体在喷嘴中的位置，以及液体的温度控制。

公开(公告)号：US20130084606A1

公开(公告)日：2013-04-04

申请号：US13703603

申请日：2011-06-22

申请人： Takaki Sugimoto ,  Tetsuya Ueda ,  Osamu Segawa ,  Michinori Koizuka ,  Hideji Tajima

发明人： Takaki Sugimoto ,  Tetsuya Ueda ,  Osamu Segawa ,  Michinori Koizuka ,  Hideji Tajima

IPC分类号： C08L91/06 ,  C12Q1/68 ,  C12P19/34

CPC分类号： C08L91/06 ,  B01L3/0275 ,  B01L3/50851 ,  B01L3/50853 ,  B01L7/52 ,  B01L2200/0673 ,  B01L2200/142 ,  C08L91/00 ,  C08L2205/02 ,  C12P19/34 ,  C12Q1/6846 ,  C12Q1/6848 ,  C12Q2547/107 ,  Y02P20/149

摘要： The present invention provides a composition capable of hermetically sealing a PCR reaction vessel without using a closure member or adhesive seal. Disclosed is a composition for preventing evaporation of a nucleic acid amplification reaction solution during nucleic acid amplification reaction, which is a liquid during the reaction and becomes a solid through chemical or thermal changes after completion of the reaction. Also disclosed is a composition for preventing evaporation of a nucleic acid amplification reaction solution during nucleic acid amplification reaction, wherein the melting point of the composition is 0-15° C. Also disclosed is a composition for preventing evaporation of a nucleic acid amplification reaction solution during nucleic acid amplification reaction, wherein the melting point of the composition is 5-10° C.

摘要翻译： 本发明提供能够不使用封闭构件或粘合剂密封而密封PCR反应容器的组合物。 公开了用于在核酸扩增反应期间防止核酸扩增反应溶液蒸发的组合物，其在反应期间为液体，并且在反应完成后通过化学或热变化而变为固体。 还公开了用于在核酸扩增反应期间防止核酸扩增反应溶液蒸发的组合物，其中组合物的熔点为0-15℃。还公开了一种用于防止核酸扩增反应溶液蒸发的组合物 在核酸扩增反应期间，其中组合物的熔点为5-10℃

公开(公告)号：US08307722B2

公开(公告)日：2012-11-13

申请号：US11920663

申请日：2006-05-19

申请人： Hideji Tajima ,  Michinori Koizuka ,  Hiroshi Suzuki ,  Susumu Kimura

发明人： Hideji Tajima ,  Michinori Koizuka ,  Hiroshi Suzuki ,  Susumu Kimura

IPC分类号： G01N1/14

CPC分类号： G01N35/1016 ,  G01N2035/1025

摘要： There are provided a method of detecting dispensed quantity in which without the need of detecting a surface of liquid accommodated in a container, a liquid quantity can be detected only by measuring pressure change; and a relevant liquid suction monitoring dispensing apparatus. There is provided a method of detecting dispensed quantity for detection of the quantity of liquid suctioned and discharged by a dispensing apparatus including a pipette tip, a liquid suctioning/discharging mechanism for the pipette tip, a pressure sensor capable of detecting the pressure within the pipette tip, and a lifting and lowering mechanism for the pipette tip, which method comprises: an insertion step for inserting a distal end of the pipette tip down to the deepest zone of a container accommodating liquid to be measured; a suction pressure measuring step for without moving of the pipette tip, while suctioning the liquid into the pipette tip at a predetermined suctioning rate, measuring any pressure change during the suctioning; and a suction detecting step for on the basis of pressure change measured, determining the state of suction. Further, there is provided a liquid suction monitoring dispensing apparatus capable of practicing this method.

摘要翻译： 提供了一种检测分配量的方法，其中不需要检测容纳在容器中的液体的表面，只能通过测量压力变化来检测液体量; 和相关的液体吸入监测分配装置。 提供了一种检测分配量的方法，用于检测由包括移液管尖端的分注装置吸取和排出的液体的量，用于移液管末端的液体吸引/排出机构，能够检测移液管内的压力的压力传感器 尖端以及用于吸头的提升和降低机构，该方法包括：插入步骤，用于将移液管尖端的远端向下插入到容纳被测量液体的容器的最深处; 抽吸压力测量步骤，用于在移动吸头的同时，以预定的抽吸速率将液体吸入移液管吸头，测量吸力期间的任何压力变化; 以及抽吸检测步骤，用于基于测量的压力变化，确定抽吸状态。 此外，提供了能够实施该方法的液体吸引监测分配装置。

公开(公告)号：US08263390B2

公开(公告)日：2012-09-11

申请号：US11792835

申请日：2005-12-12

申请人： Hideji Tajima

发明人： Hideji Tajima

IPC分类号： C12M1/34 ,  G01N21/01

CPC分类号： B01L3/502 ,  B01L3/0275 ,  B01L2200/0631 ,  B01L2200/0647 ,  B01L2300/0609 ,  B01L2300/0636 ,  B01L2300/12 ,  G01N35/10 ,  G01N2035/1053

摘要： A biological material fixed carrier enclosing tip, a biological material fixed carrier treatment apparatus, and a treatment method thereof. An object is to obviate attachment control and suction control for storing and retaining the carrier in the tip form vessel, to simplify complex reaction processes, and to make processing of the biological material fixed carrier to be easily executed as a result of a small-scale device configuration. The biological material fixed carrier enclosing tip comprises: a tip form vessel having an installation opening part that is installable to a nozzle that performs suction and discharge of gas, and a narrow tube that possesses an opening, through which fluid inflow and outflow is possible by means of the suction and discharge of gas, that is narrower than the nozzle; a carrier in which a predetermined biological material is fixed or fixable in a plurality of different positions that are determined beforehand that are distinguishable from the exterior, and has a size or a shape that is able to pass through the opening; and an enclosing section provided on the tip form vessel that encloses the carrier within the narrow tube in a state where it is able to make contact with the fluid that has flown into the narrow tube from the opening.

摘要翻译： 生物材料固定载体包封尖端，生物材料固定载体处理装置及其处理方法。 目的是消除用于将载体储存和保持在尖端形状容器中的附接控制和抽吸控制，以简化复杂的反应过程，并且使生物材料固定载体的处理由于小规模化而容易地执行 设备配置。 生物材料固定载体包封尖端包括：尖端形状容器，其具有可安装到执行气体的抽吸和排出的喷嘴的安装开口部分和具有开口的窄管，通过该狭窄管可以通过该开口流体流出和流出 气体的吸入和排出装置，比喷嘴窄; 其中预定生物材料固定或固定在预先确定的与外部可区分的多个不同位置的载体，并且具有能够穿过开口的尺寸或形状; 以及设置在尖端形式容器上的封闭部分，其在能够与从开口流入细管的流体接触的状态下将载体包围在窄管内。

公开(公告)号：US20120190034A1

公开(公告)日：2012-07-26

申请号：US13388885

申请日：2010-08-05

申请人： Hideji Tajima

发明人： Hideji Tajima

IPC分类号： G01N21/76 ,  C12M1/34

CPC分类号： G01N21/6452 ,  G01J3/0208 ,  G01J3/021 ,  G01J3/0218 ,  G01J3/0235 ,  G01J3/024 ,  G01J3/0291 ,  G01J3/08 ,  G01J3/10 ,  G01J3/32 ,  G01J3/4406 ,  G01J2003/1213 ,  G01N21/253 ,  G01N35/028 ,  G01N2021/6484 ,  G01N2201/0826 ,  G01N2201/0833 ,  G02B6/4298

摘要： Disclosed is a highly reliable optical fiber measurement device and measurement method having a simple and compact structure. The device includes a planar liquid holder having a plurality of liquid holding portions arranged along a flat face; a plurality of light receiving optical fibers for transmitting fluorescence generated in the liquid holding portions; a plurality of light emitting optical fibers for transmitting excitation light into the liquid holding portions; a measurement head capable of being positioned in the each liquid holding portion while supporting a plurality of measurement ends having a bundle of one light receiving end of the light receiving optical fibers and one light emitting end of light emitting optical fibers; a light reception selecting element that, by sequentially selecting one by one from plural the light receiving optical fibers and sequentially selecting one by one from plural kinds of wavelength or wavelength bands, sequentially guides the light of the selected wavelength or wavelength band of the fluorescence received by the selected light receiving optical fibers to one photoelectric element; and a photoelectric element for sequentially conducting photoelectric conversion on the guided fluorescence.

摘要翻译： 公开了一种具有简单紧凑结构的高度可靠的光纤测量装置和测量方法。 该装置包括具有沿着平坦表面布置的多个液体保持部分的平面液体保持器; 多个用于透射在液体保持部分中产生的荧光的光接收光纤; 用于将激发光发射到液体保持部分中的多个发光光纤; 测量头，其能够定位在每个液体保持部分中，同时支撑具有光接收光纤的一个光接收端的束和发光光纤的一个发光端的多个测量端; 光接收选择元件，通过从多个光接收光纤中逐一选择并从多种波长或波长带中逐个选择一个顺序地选择所接收的荧光的所选波长或波长带的光， 通过所选择的光接收光纤到一个光电元件; 以及用于对引导荧光进行光电转换的光电元件。

公开(公告)号：US08168142B2

公开(公告)日：2012-05-01

申请号：US11568112

申请日：2005-04-20

申请人： Hideji Tajima ,  Tsutomu Asano ,  Junko Asahina ,  Mamiko Yoshida

发明人： Hideji Tajima ,  Tsutomu Asano ,  Junko Asahina ,  Mamiko Yoshida

IPC分类号： A61J1/06 ,  G01N37/00

CPC分类号： G01N35/00009 ,  B01L3/0244 ,  B01L3/0248 ,  B01L3/0255 ,  B01L3/0275 ,  B01L3/0293 ,  B01L3/508 ,  B01L9/00 ,  B01L2300/0812 ,  B01L2300/0845 ,  G01N2035/1039

摘要： It is an object of this invention to provide a sample integration cassette, a spotting device and a sample integration device which enhance a sample integration density and reduce the size of a sample assembly and a probe. The sample integration cassette has the sample assembly and the sample support carrier so arranged therein that the sample support can be fed from the sample support carrier onto the sample assembly at a predetermined angle to the sample assembly at all times. The cassette is formed so as to be connectable with a drive unit that applies a drive force to the sample assembly and the sample support carrier interlockingly and synchronously. The spotting device has a delivery member mounting table which is vertically moved over the sample support carrier to apply samples to the sample support from the delivery member. The sample integration device rotatably supports the sample support carrier and includes a rotary drive unit for rotating the sample support carrier and the sample assembly and a biasing means connected to the rotary drive unit to pull the cassette in one direction. The sample assembly rotating device includes a rotating shaft to which the sample assembly is fitted at one end, a drive means to drive the rotating shaft, and a power supply for the drive means.

摘要翻译： 本发明的目的是提供一种样品集成盒，点样装置和样品一体化装置，其增强样品集成密度并减小样品组件和探针的尺寸。 样品积分盒具有样品组件和样品支撑载体，其布置在其中，使得样品载体可以始终以与样品组件预定的角度从样品支撑载体供给到样品组件上。 盒体形成为可与驱动单元连接，该驱动单元将样品组件和样品支撑载体互锁并同步地施加驱动力。 点样装置具有一个传送部件安装台，该传送部件安装台垂直移动到样品支撑载体上，以将样品从传送部件施加到样品支架上。 样品积分装置可旋转地支撑样品支撑载体，并且包括旋转驱动单元，用于使样品支撑载体和样品组件旋转，以及连接到旋转驱动单元以沿一个方向拉动盒的偏置装置。 样品组件旋转装置包括一个旋转轴，样品组件在其一端装配到该旋转轴上，驱动装置驱动旋转轴，以及驱动装置的电源。

公开(公告)号：US20110262919A1

公开(公告)日：2011-10-27

申请号：US13132041

申请日：2009-12-25

申请人： Hideji Tajima

发明人： Hideji Tajima

IPC分类号： C12Q1/68 ,  G01N21/64 ,  C12M1/34 ,  G01N21/00 ,  G01N33/543 ,  G01N1/28

CPC分类号： G01N33/54386 ,  B01L3/508 ,  B01L3/52 ,  B01L2200/10 ,  B01L2300/0636 ,  C12Q1/6804 ,  G01N33/54326 ,  G01N33/54393 ,  G01N35/026 ,  G01N35/1009 ,  Y10T436/143333 ,  Y10T436/25125 ,  C12Q2563/143

摘要： On magnetic particles serving as a first support, an antibody against a nonspecific reaction factor is immobilized. These magnetic particles are mixed with a specimen and suspended therein. After suspending, the suspension is sucked up into a pipette chip and a magnet comes close to the pipette chip. While remotely constraining with the magnet the magnetic particles carrying the nonspecific reaction factor bonded thereto, the residual liquid is discharged into a well. Thus, the removal of a contaminant contained in the specimen is completed. The thus treated specimen discharged into the well is subjected to an immunoassay. The magnetic particles carrying the antibody immobilized thereon are mixed with the treated specimen and suspended therein. The suspension is sucked up into a pipette chip and the magnetic particles carrying an antigen bonded thereto are separated by use of the magnet. The magnetic particles carrying the antigen bonded thereto are washed, mixed with an enzyme-labeling solution, which contains a second support, and suspended therein. After suspending, the magnetic particles being labeled and carrying the antigen bonded thereto are mixed with a substrate solution and subjected to the measurement of emission intensity, etc.

摘要翻译： 在用作第一载体的磁性颗粒上，固定抗非特异性反应因子的抗体。 这些磁性颗粒与样品混合并悬浮在其中。 悬挂后，将悬浮液吸入移液管芯片，磁铁靠近移液管芯片。 当与磁体远程约束时，携带非特异性反应因子的磁性颗粒与其结合，残留的液体被排放到井中。 因此，完成了包含在样品中的污染物的去除。 将如此处理的样品排出到孔中进行免疫测定。 将固定有抗体的磁性颗粒与经处理的样品混合并悬浮在其中。 将悬浮液吸入移液管芯片中，并且携带与其结合的抗原的磁性颗粒通过使用磁体分离。 将携带与其键合的抗原的磁性颗粒洗涤，与含有第二载体的酶标记溶液混合并悬浮在其中。 悬浮后，将标记的磁性颗粒与其结合的抗原与底物溶液混合并进行发射强度等的测定。

公开(公告)号：US07951335B2

公开(公告)日：2011-05-31

申请号：US12776085

申请日：2010-05-07

申请人： Hideji Tajima

发明人： Hideji Tajima

IPC分类号： G01N21/00 ,  G01N21/01 ,  B01L11/00 ,  B01J19/00

CPC分类号： G01N21/03 ,  B01L3/0275 ,  B01L3/502 ,  B01L3/5021 ,  B01L2200/0621 ,  B01L2400/0409 ,  B01L2400/0487 ,  B01L2400/0655

摘要： The invention relates to a reaction vessel, a reaction vessel liquid introducing device, a liquid introducing and reaction measuring device, and a liquid introducing device, and is directed to being able to perform temperature control of a liquid stored within the reaction vessel with a high accuracy and faithful responsiveness. The reaction vessel comprises: a storage chamber in which a liquid is storable, that has an opening part; a reaction chamber that is formed thinner or narrower than the storage chamber; and at least one flow passage that communicates between the storage chamber or the exterior and the reaction chamber. The reaction vessel is formed such that it is connectable to a liquid introducing section provided externally, and the liquid can be introduced into the reaction chamber by connecting to the liquid introducing section.

公开(公告)号：US20100163111A1

公开(公告)日：2010-07-01

申请号：US12450672

申请日：2008-04-07

申请人： Hideji Tajima

发明人： Hideji Tajima

IPC分类号： F16L53/00

CPC分类号： B01L7/525 ,  B01L3/021 ,  B01L3/0275 ,  B01L7/52 ,  B01L2200/0684 ,  B01L2200/142 ,  B01L2200/147 ,  B01L2300/0636 ,  B01L2300/0838 ,  B01L2300/18 ,  B01L2300/1822 ,  B01L2300/1844 ,  B01L2300/1888 ,  B01L2400/0478 ,  B01L2400/0487 ,  G01N2035/00346 ,  G01N2035/103 ,  G01N2035/1039 ,  G01N2035/1062 ,  Y10T137/0329 ,  Y10T137/6552

摘要： The present invention relates to a temperature controlling apparatus and the temperature controlling method which can arrest foaming and inflation of the air contained in a liquid, and can perform temperature controlling of a liquid of high reliance, and the present invention is constructed to have one or a plurality of sets of nozzles which can suck and discharge a liquid through a tip, and can retain the sucked liquid, a suction and discharge mechanism which can suck and discharge a gas via the each nozzle, a temperature regulator which can maintain one or two or more set predetermined temperatures for a predetermined time regarding one or two or more predetermined temperature controlling regions set in the each nozzle, a movement mechanism which allows relative movement between one or two or more containers which can accommodate a liquid and a nozzle, and a controlling part which instructs the movement mechanism, the suction and discharge mechanism or the temperature regulator to adjust a liquid to be sucked into the nozzle and a liquid amount thereof, a position of the sucked liquid in the nozzle, and temperature controlling of the liquid.

摘要翻译： 本发明涉及一种温度控制装置和温度控制方法，其可以阻止液体中包含的空气的起泡和膨胀，并且可以执行高依赖性的液体的温度控制，并且本发明构造成具有一个或 多个喷嘴组，可以通过尖端吸取和排出液体，并且可以保持被吸入的液体;吸入和排出机构，其可以通过每个喷嘴吸入和排出气体;温度调节器，其可以保持一个或两个 或更多的预定温度对于设置在每个喷嘴中的一个或两个或更多个预定温度控制区域设定预定温度;允许一个或两个或更多个可容纳液体和喷嘴的容器之间的相对运动的移动机构，以及 控制部分，指示移动机构，吸入和排出机构或温度调节器 仅吸入喷嘴的液体及其液体量，吸入液体在喷嘴中的位置，以及液体的温度控制。