公开(公告)号：US11779897B2

公开(公告)日：2023-10-10

申请号：US17515511

申请日：2021-10-31

Applicant: ILLUMINA, INC.

Inventor： Lewis J. Kraft ,  Tarun Kumar Khurana ,  Yir-Shyuan Wu ,  Xi-Jun Chen ,  Arnaud Rival ,  Justin Fullerton ,  M. Shane Bowen ,  Hui Han ,  Jeffrey S. Fisher ,  Yasaman Farshchi ,  Mathieu Lessard-Viger

IPC: B01J19/00 ,  C12N15/10 ,  C40B40/08 ,  B01L3/00 ,  C40B50/18 ,  C08L37/00 ,  G01N21/05

CPC classification number: B01J19/0046 ,  B01L3/5027 ,  B01L3/502707 ,  C12N15/1065 ,  C40B40/08 ,  C40B50/18 ,  B01J2219/00286 ,  B01J2219/00608 ,  B01L2300/0819 ,  B01L2300/0877 ,  C08L37/00 ,  G01N2021/058

Abstract: An example of a flow cell includes a substrate, a plurality of chambers defined on or in the substrate, and a plurality of depressions defined in the substrate and within a perimeter of each of the plurality of chambers. The depressions are separated by interstitial regions. Primers are attached within each of the plurality of depressions, and a capture site is located within each of the plurality of chambers.

公开(公告)号：US20220367547A1

公开(公告)日：2022-11-17

申请号：US17804023

申请日：2022-05-25

Applicant: Illumina, Inc.

Inventor： Arvin Emadi ,  Arnaud Rival ,  Ali Agah

IPC: H01L27/146 ,  H01L23/00

Abstract: Disclosed in one example is an apparatus including a substrate, a sensor over the substrate including an active surface and a sensor bond pad, a molding layer over the substrate and covering sides of the sensor, the molding layer having a molding height relative to a top surface of the substrate that is greater than a height of the active surface of the sensor relative to the top surface of the substrate, and a lidding layer over the molding layer and over the active surface. The lidding layer and the molding layer form a space over the active surface of the sensor that defines a flow channel.

公开(公告)号：US11385162B2

公开(公告)日：2022-07-12

申请号：US17148214

申请日：2021-01-13

Applicant: Illumina, Inc.

Inventor： Arnaud Rival ,  Ali Agah ,  Tracy H. Fung ,  Dietrich Dehlinger ,  Poorya Sabounchi ,  Tarun Khurana ,  Craig M. Ciesla ,  M. Shane Bowen

IPC: G01N15/14

Abstract: Flow cells and corresponding methods are provided. The flow cells may include a support frame with top and back sides, and at least one cavity extending from the top side. The flow cells may include at least one light detection device with an active area disposed within the at least one cavity. The flow cells may include a support material disposed within the at least one cavity between the support frame and the periphery of the at least one light detection device coupling them together. The flow cells may include a lid extending over the at least one light detection device and coupled to the support frame about the periphery of the at least one light detection device. The lid and at least a top surface of the at least one light detection device form a flow channel therebetween.

公开(公告)号：US20210379578A1

公开(公告)日：2021-12-09

申请号：US17324363

申请日：2021-05-19

Applicant: ILLUMINA, INC.

Inventor： Wesley Cox-Muranami ,  Rajagopal Panchapakesan ,  Arnaud Rival

IPC: B01L3/00 ,  B01L7/00 ,  C12Q1/6869

Abstract: An apparatus and examples of methods for using and manufacturing aspects of an apparatus with a sensor having an active surface. A sensor, a lid, and a flow channel bounded by the lid and a surface of the sensor, and including an illumination source, a heater, and a pump. A method includes fluidically coupling a first flow cell and a second flow cell to a reservoir, moving fluid from the reservoir into a flow channel of the first and second flow cell using respective pumps; and heating fluid in the flow channels of the first and second flow cells using respective heaters. A method includes forming a first sensor and a second sensor on a flexible surface, and folding the flexible surface until the first sensor faces the second sensor.

公开(公告)号：US11192083B2

公开(公告)日：2021-12-07

申请号：US16750831

申请日：2020-01-23

Applicant: ILLUMINA, INC.

Inventor： Lewis J. Kraft ,  Tarun Kumar Khurana ,  Yir-Shyuan Wu ,  Xi-Jun Chen ,  Arnaud Rival ,  Justin Fullerton ,  M. Shane Bowen ,  Hui Han ,  Jeffrey S. Fisher ,  Yasaman Farshchi ,  Mathieu Lessard-Viger

IPC: B01J19/00 ,  C12N15/10 ,  C40B40/08 ,  B01L3/00 ,  C40B50/18 ,  C08L37/00 ,  G01N21/05

Abstract: An example of a flow cell includes a substrate, a plurality of chambers defined on or in the substrate, and a plurality of depressions defined in the substrate and within a perimeter of each of the plurality of chambers. The depressions are separated by interstitial regions. Primers are attached within each of the plurality of depressions, and a capture site is located within each of the plurality of chambers.

公开(公告)号：US10921233B2

公开(公告)日：2021-02-16

申请号：US16567224

申请日：2019-09-11

Applicant: Illumina, Inc.

Inventor： Arnaud Rival ,  Ali Agah ,  Tracy H. Fung ,  Dietrich Dehlinger ,  Poorya Sabounchi ,  Tarun Khurana ,  Craig M. Ciesla ,  M. Shane Bowen

IPC: G01N15/14

Abstract: Flow cells and corresponding methods are provided. The flow cells may include a support frame with top and back sides, and at least one cavity extending from the top side. The flow cells may include at least one light detection device with an active area disposed within the at least one cavity. The flow cells may include a support material disposed within the at least one cavity between the support frame and the periphery of the at least one light detection device coupling them together. The flow cells may include a lid extending over the at least one light detection device and coupled to the support frame about the periphery of the at least one light detection device. The lid and at least a top surface of the at least one light detection device form a flow channel therebetween.

公开(公告)号：US20200238276A1

公开(公告)日：2020-07-30

申请号：US16750930

申请日：2020-01-23

Applicant: ILLUMINA, INC.

Inventor： Tarun Kumar Khurana ,  Arnaud Rival ,  Lewis J. Kraft ,  Steven Barnard ,  M. Shane Bowen ,  Xi-Jun Chen ,  Yir-Shyuan Wu ,  Jeffrey S. Fisher ,  Dajun Yuan

IPC: B01L3/00 ,  C12N15/10

Abstract: An example of a flow cell includes a substrate, which includes nano-depressions defined in a surface of the substrate, and interstitial regions separating the nano-depressions. A hydrophobic material layer has a surface that is at least substantially co-planar with the interstitial regions and is positioned to define a hydrophobic barrier around respective sub-sets of the nano-depressions.

公开(公告)号：US20200238247A1

公开(公告)日：2020-07-30

申请号：US16750831

申请日：2020-01-23

Applicant: ILLUMINA, INC.

Inventor： Lewis J. Kraft ,  Tarun Kumar Khurana ,  Yir-Shyuan Wu ,  Xi-Jun Chen ,  Arnaud Rival ,  Justin Fullerton ,  M. Shane Bowen ,  Hui Han ,  Jeffrey S. Fisher ,  Yasaman Farshchi ,  Mathieu Lessard-Viger

IPC: B01J19/00 ,  C12N15/10 ,  C40B40/08 ,  C40B50/18 ,  B01L3/00

Abstract: An example of a flow cell includes a substrate, a plurality of chambers defined on or in the substrate, and a plurality of depressions defined in the substrate and within a perimeter of each of the plurality of chambers. The depressions are separated by interstitial regions. Primers are attached within each of the plurality of depressions, and a capture site is located within each of the plurality of chambers.