公开(公告)号：US20120217761A1

公开(公告)日：2012-08-30

申请号：US13403269

申请日：2012-02-23

Applicant: Jun Nakamura ,  Naoki Shishida

Inventor： Jun Nakamura ,  Naoki Shishida

IPC: B62D25/12 ,  B62D25/10

CPC classification number: E05B83/24 ,  B60R21/34 ,  B60R2021/343 ,  B62D25/105 ,  E05B77/02 ,  E05B77/10 ,  Y10T292/0946

Abstract: A front body structure for a vehicle includes a striker bracket provided in a hood frame, and a frame reinforcing unit provided in front of the striker bracket to reinforce the hood frame. In addition to, or alternatively, a reinforcement is arranged between the frame and a at least a portion of the hood skin.

Abstract translation: 一种用于车辆的前身体结构包括设置在发动机罩框架中的撞针架，以及设置在撞针支架前方以加强罩框架的框架加强单元。 除了或者替代地，在框架和发动机皮肤的至少一部分之间布置加强件。

公开(公告)号：US07927844B2

公开(公告)日：2011-04-19

申请号：US12550929

申请日：2009-08-31

Applicant: Jun Nakamura ,  Seiko Hirano ,  Hisao Ito

Inventor： Jun Nakamura ,  Seiko Hirano ,  Hisao Ito

IPC: C12P13/14 ,  C12N1/21

CPC classification number: C12P13/14 ,  C07K14/34

Abstract: A coryneform bacterium that is modified by using a yggB gene so that L-glutamic acid-producing ability is enhanced as compared to a non-modified strains is cultured in a medium to cause accumulation of L-glutamic acid in the medium or bacterial cells, and L-glutamic acid is collected from the medium or cells.

Abstract translation: 通过在培养基中培养通过使用yggB基因修饰的棒状细菌，使得与非修饰菌株相比增强L-谷氨酸生产能力，以在培养基或细菌细胞中引起L-谷氨酸的积累， 从培养基或细胞收集L-谷氨酸。

公开(公告)号：US07829316B2

公开(公告)日：2010-11-09

申请号：US12104595

申请日：2008-04-17

Applicant: Chie Koseki ,  Keita Fukui ,  Jun Nakamura ,  Hiroyuki Kojima

Inventor： Chie Koseki ,  Keita Fukui ,  Jun Nakamura ,  Hiroyuki Kojima

IPC: C12P7/46 ,  C12N9/04 ,  C12N15/00 ,  C12N15/74 ,  C12N1/20 ,  C12Q1/32 ,  C07H21/04

CPC classification number: C12P7/46

Abstract: Disclosed is a process for production of succinic acid, which comprises the step of reacting a bacterium which has been modified so as to increase the expression of a sucE1 gene or a product produced by any treatment of the bacterium with an organic raw material in a reaction solution containing a carbonate ion, a bicarbonate ion or carbon dioxide gas to thereby yield the desired succinic acid.

Abstract translation: 公开了一种生产琥珀酸的方法，其包括使已经被修饰以增加sucE1基因的表达的细菌或通过任何处理细菌产生的产物与反应中的有机原料反应的步骤 含有碳酸根离子，碳酸氢根离子或二氧化碳气体的溶液，从而得到所需的琥珀酸。

公开(公告)号：US20090233307A1

公开(公告)日：2009-09-17

申请号：US12404532

申请日：2009-03-16

Applicant: Jun Nakamura ,  April Luke ,  James Swenberg

Inventor： Jun Nakamura ,  April Luke ,  James Swenberg

IPC: C12Q1/68 ,  G01N33/00

CPC classification number: C12Q1/6806 ,  Y10T436/143333 ,  C12Q2527/137 ,  C12Q2527/125 ,  C12Q2527/119

Abstract: Methods are provided for detecting single strand breaks in DNA sample comprising: contacting the DNA with a neutral hydroxylamine, denaturing the DNA and detecting single strand breaks in the denatured DNA. Methods are also provided for the detecting single strand breaks in DNA from a cell, the methods comprising lysing the cell, denaturing the DNA from the lysed cell, wherein the DNA is contacted with a neutral hydroxylamine prior to and/or during the denaturing step and detecting single strand breaks in the DNA from the cell. In addition, methods are provided for distinguishing between true single strand breaks and artifactual single strand breaks resulting from cleaved alkaline labile sites.

Abstract translation: 提供了用于检测DNA样品中单链断裂的方法，包括：使DNA与中性羟胺接触，使DNA变性并检测变性DNA中的单链断裂。 还提供了用于检测来自细胞的DNA中的单链断裂的方法，所述方法包括裂解细胞，使来自裂解细胞的DNA变性，其中DNA在变性步骤之前和/或期间与中性羟胺接触，以及 从细胞中检测DNA中的单链断裂。 此外，提供了用于区分真实单链断裂和由裂解的碱性不稳定位点产生的人为因素的单链断裂的方法。

公开(公告)号：US07559070B2

公开(公告)日：2009-07-07

申请号：US10565983

申请日：2004-07-27

Applicant: Jun Nakamura ,  Hideo Saito ,  Kazuhiro Hiramoto ,  Yuichi Sei ,  Sergey Kozhevnikov ,  Takuro Matsuda ,  Jun Togo ,  Shingo Ono ,  Seiya Kato ,  Yutaka Arakawa

Inventor： Jun Nakamura ,  Hideo Saito ,  Kazuhiro Hiramoto ,  Yuichi Sei ,  Sergey Kozhevnikov ,  Takuro Matsuda ,  Jun Togo ,  Shingo Ono ,  Seiya Kato ,  Yutaka Arakawa

IPC: H04N7/167 ,  H04N7/10

CPC classification number: H04N7/167 ,  H04N7/162 ,  H04N7/17318 ,  H04N21/234327 ,  H04N21/23476 ,  H04N21/2389 ,  H04N21/4385 ,  H04N21/44055 ,  H04N21/4622 ,  H04N21/47202 ,  H04N21/4782 ,  H04N21/6125 ,  H04N21/631 ,  H04N21/812

Abstract: A moving image distribution system, wherein the user can view content moving images at no charge, and the provider of the content moving images can prevent a drain of the moving image contents and can protect the copy right thereof, as a copy right holder, without fail. The moving images are divided, along a time axis, into two master and slave nonreproducible moving image files. The slave moving image file (small file) is encrypted based on CM file data in which a predetermined CM element has been incorporated. In response to a reproduction request of a user terminal that possesses the master moving image file (main file) in advance, the slave moving image file is distributed with the CM moving images to the user terminal via a network line such as the Internet.

Abstract translation: 一种运动图像分配系统，其中用户可以免费观看内容运动图像，并且内容运动图像的提供者可以防止运动图像内容的消除，并且可以保护作为副本权利持有者的副本权利，而没有 失败。 沿着时间轴将运动图像分割成两个主和从不可重现的运动图像文件。 基于已经包含预定CM元素的CM文件数据对从动运动图像文件（小文件）进行加密。 响应于预先拥有主动图像文件（主文件）的用户终端的再现请求，从动运动图像文件通过诸如因特网的网络将CM运动图像分发给用户终端。

公开(公告)号：US20070092953A1

公开(公告)日：2007-04-26

申请号：US11616463

申请日：2006-12-27

Applicant: Jun Nakamura ,  Hiroshi Izui ,  Kayo Moriguchi ,  Hiroki Kawashima ,  Tsuyoshi Nakamatsu ,  Osamu Kurahashi

Inventor： Jun Nakamura ,  Hiroshi Izui ,  Kayo Moriguchi ,  Hiroki Kawashima ,  Tsuyoshi Nakamatsu ,  Osamu Kurahashi

IPC: C12P13/14 ,  C12N1/21

CPC classification number: C12N9/0016 ,  C12N9/1241 ,  C12N9/93 ,  C12P13/04 ,  C12P13/14

Abstract: L-Glutamine is produced by culturing a coryneform bacterium which has L-glutamine producing ability and has been modified so that its intracellular glutamine synthetase activity should be enhanced, preferably which has been further modified so that its intracellular glutamate dehydrogenase activity should be enhanced, in a medium to produce and accumulate L-glutamine in the medium and collecting the L-glutamine.

Abstract translation: L-谷氨酰胺通过培养具有L-谷氨酰胺生产能力并已被修饰以使其细胞内谷氨酰胺合成酶活性得到增强的棒状细菌产生，优选已被进一步修饰以使其细胞内谷氨酸脱氢酶活性应当增强， 在培养基中产生和积累L-谷氨酰胺并收集L-谷氨酰胺的培养基。

公开(公告)号：US20060205048A1

公开(公告)日：2006-09-14

申请号：US11362931

申请日：2006-02-28

Applicant: Makoto Murase ,  Ryusuke Aoyama ,  Miki Ikuta ,  Kenji Yamagishi ,  Mika Moriya ,  Jun Nakamura ,  Hiroyuki Kojima

Inventor： Makoto Murase ,  Ryusuke Aoyama ,  Miki Ikuta ,  Kenji Yamagishi ,  Mika Moriya ,  Jun Nakamura ,  Hiroyuki Kojima

IPC: C12P7/62 ,  C12P7/46 ,  C12N1/21

CPC classification number: C12P7/46

Abstract: Succinic acid is produced by allowing a bacterium modified to enhance fumarate reductase activity or cell preparation thereof to react with an organic raw material in a reaction solution containing one of a carbonate ion, a bicarbonate ion, and carbon dioxide gas to generate succinic acid. More preferably, succinic acid is produced by allowing a bacterium modified to enhance activities of fumarate reductase and pyruvate carboxylase and decrease lactate dehydrogenase activity or cell preparation thereof to react with an organic raw material in a reaction solution containing one of a carbonate ion, a bicarbonate ion, and carbon dioxide gas to generate succinic acid. Succinic acid is obtained by collecting the produced succinic acid.

Abstract translation: 琥珀酸是通过使经修饰的细菌增强富马酸盐还原酶活性或其制备方法与含有碳酸根离子，碳酸氢根离子和二氧化碳气体的反应溶液中的有机原料反应生成琥珀酸而制备的。 更优选地，通过使经修饰的细菌增强富马酸盐还原酶和丙酮酸羧化酶的活性并降低乳酸脱氢酶活性或其细胞制备，在含有碳酸根离子，碳酸氢盐 离子和二氧化碳气体产生琥珀酸。 通过收集产生的琥珀酸获得琥珀酸。

公开(公告)号：US20060003424A1

公开(公告)日：2006-01-05

申请号：US11218433

申请日：2005-09-06

Applicant: Yoko Asakura ,  Jun Nakamura ,  Sohei Kanno ,  Mikiko Suga ,  Eiichiro Kimura ,  Hisao Ito ,  Kazuhiko Matsui ,  Tsuyoshi Ohsumi ,  Tsuyoshi Nakamatsu ,  Osamu Kurahashi

Inventor： Yoko Asakura ,  Jun Nakamura ,  Sohei Kanno ,  Mikiko Suga ,  Eiichiro Kimura ,  Hisao Ito ,  Kazuhiko Matsui ,  Tsuyoshi Ohsumi ,  Tsuyoshi Nakamatsu ,  Osamu Kurahashi

IPC: C12P13/22 ,  C12P13/14 ,  C12P13/08 ,  C12N1/21

CPC classification number: C12P13/14 ,  C12N15/67

Abstract: A method of producing coryneform bacteria having an improved amino acid- or nucleic acid-productivity comprises the steps of introducing a mutation in a promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on a chromosome of a coryneform bacterium to make it close to a consensus sequence or introducing a change in a promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on a chromosome of a coryneform bacterium by gene recombination to make it close to a consensus sequence, to obtain mutants of the coryneform amino acid d- or nucleic acid-producing microorganism, culturing the mutants and select a mutant capable of producing the intended amino acid or nucleic acid in a large amount. This method can construct a mutant capable of suitably enriching or controling the expression of an intended gene without using a plasmid and also capable of producing amino acids in a high yield, by the recombination or mutation.

Abstract translation: 具有改善的氨基酸或核酸生产力的棒状细菌的方法包括以下步骤：在棒状细菌的染色体上的氨基酸或核酸 - 生物合成基因的启动子序列中引入突变，使其接近 共有序列或通过基因重组在棒状细菌的染色体上引入氨基酸或核酸 - 生物合成基因的启动子序列的变化，使其接近共有序列，获得棒状杆菌氨基酸d- 或产生核酸的微生物，培养突变体并选择能够大量产生所需氨基酸或核酸的突变体。 该方法可以构建能够适当地富集或控制预期基因的表达而不使用质粒并且还能够通过重组或突变以高产率产生氨基酸的突变体。

公开(公告)号：US06962805B2

公开(公告)日：2005-11-08

申请号：US09577005

申请日：2000-05-25

Applicant: Yoko Asakura ,  Jun Nakamura ,  Sohei Kanno ,  Mikiko Suga ,  Eiichiro Kimura ,  Hisao Ito ,  Kazuhiko Matsui ,  Tsuyoshi Ohsumi ,  Tsuyoshi Nakamatsu ,  Osamu Kurahashi

Inventor： Yoko Asakura ,  Jun Nakamura ,  Sohei Kanno ,  Mikiko Suga ,  Eiichiro Kimura ,  Hisao Ito ,  Kazuhiko Matsui ,  Tsuyoshi Ohsumi ,  Tsuyoshi Nakamatsu ,  Osamu Kurahashi

IPC: C12N15/37 ,  C12N1/21 ,  C12N15/67 ,  C12P13/04 ,  C12P13/14 ,  C07H21/04 ,  C12N1/20 ,  C12N15/74 ,  C12Q1/32

CPC classification number: C12P13/14 ,  C12N15/67

Abstract: A method of producing coryneform bacteria having improved amino acid or nucleic acid productivity comprising the steps of introducing a mutation in a promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on a chromosome of a coryneform bacterium to make it close to a consensus sequence, or introducing a change in a promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on a chromosome of a coryneform bacterium by gene recombination to make it close to a consensus sequence, to obtain mutants of the coryneform amino acid- or nucleic acid-producing microorganism, culturing the mutants and selecting a mutant capable of producing the intended amino acid or nucleic acid in a large amount. This method allows the construction of a mutant capable of enriching or controlling the expression of an intended gene without using a plasmid and to promote production of amino acids in a high yield by recombination or mutation.

Abstract translation: 制备具有改善的氨基酸或核酸生产力的棒状细菌的方法包括以下步骤：在棒状细菌染色体上的氨基酸或核酸 - 生物合成基因的启动子序列中引入突变，使其接近共有序列 或通过基因重组在棒状细菌的染色体上引入氨基酸或核酸 - 生物合成基因的启动子序列的变化使其接近共有序列，以获得棒状细胞氨基酸或核酸的突变体 培养微生物，培养突变体并选择能够大量产生所需氨基酸或核酸的突变体。 该方法允许构建能够富集或控制预期基因的表达而不使用质粒的突变体，并通过重组或突变促进高产量的氨基酸的产生。

公开(公告)号：US20050014236A1

公开(公告)日：2005-01-20

申请号：US10790224

申请日：2004-03-02

Applicant: Yumi Matsuzaki ,  Jun Nakamura ,  Kenichi Hashiguchi

Inventor： Yumi Matsuzaki ,  Jun Nakamura ,  Kenichi Hashiguchi

IPC: C12N1/20 ,  C12N1/21 ,  C12P13/08 ,  C12P13/10 ,  C12N9/10

CPC classification number: C12P13/10 ,  C12P13/08

Abstract: L-arginine or L-lysine is produced by culturing a coryneform bacterium having an L-arginine- or L-lysine-producing ability and modified so that glutamine synthetase activity is enhanced, e.g., a coryneform bacterium which is modified so that adenylylation of glutamine synthetase is eliminated. L-arginine or L-lysine are produced by culturing the bacterium in a medium and allowing L-arginine or L-lysine to accumulate in the medium, and collecting the L-arginine or L-lysine from the medium.

Abstract translation: 通过培养具有L-精氨酸或L-赖氨酸生产能力的棒状细菌产生L-精氨酸或L-赖氨酸，并对其进行了修饰，使得谷氨酰胺合成酶活性得到增强，例如，修饰为使得谷氨酰胺腺苷酰化的棒状细菌 合成酶被消除。 通过在培养基中培养细菌并使L-精氨酸或L-赖氨酸在培养基中积聚并从培养基中收集L-精氨酸或L-赖氨酸来产生L-精氨酸或L-赖氨酸。