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52 条记录 (耗时 0.037 秒)

    Method for synthesizing DNA
    31.
    发明授权
    Method for synthesizing DNA 有权
    DNA合成方法

    公开(公告)号：US07521178B1

    公开(公告)日：2009-04-21

    申请号：US09673884

    申请日：1999-04-21

    申请人： Kiyozo Asada ,  Takashi Uemori ,  Yoshimi Sato ,  Tomoko Fujita ,  Kazue Miyake ,  Osamu Takeda ,  Hiroyuki Mukai ,  Ikunoshin Kato

    发明人： Kiyozo Asada ,  Takashi Uemori ,  Yoshimi Sato ,  Tomoko Fujita ,  Kazue Miyake ,  Osamu Takeda ,  Hiroyuki Mukai ,  Ikunoshin Kato

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02

    CPC分类号： C12P19/34 ,  C12N9/1252 ,  C12N9/96 ,  C12Q1/6806 ,  Y10S435/975 ,  C12Q2521/107

    摘要： A DNA synthesis reaction-enhancer comprising at least one kind selected from the group consisting of acidic substances and cationic complexes; a DNA synthesis method in which during a DNA synthesis reaction a reaction is carried out in the presence of the above enhancer by using DNA polymerase; a DNA synthesis reaction composition comprising the above enhancer; a DNA synthesis reaction composition comprising two or more kinds of DNA polymerases each having 3′→5′ exonuclease activity; a DNA synthesis method in which during a DNA synthesis reaction two or more kinds of DNA polymerases each having 3′→5′ exonuclease activity are used; a kit for use in in vitro DNA synthesis, comprising two or more kinds of DNA polymerases each having 3′→5′ exonuclease activity; and a kit for use in in vitro DNA synthesis, wherein the kit comprises the DNA synthesis reaction-enhancer and DNA polymerase. According to the present invention, DNA synthesis can be carried out at an efficiency more excellent as compared to conventional DNA synthesis reaction.

    摘要翻译： 一种DNA合成反应增强剂，其包含选自酸性物质和阳离子配合物中的至少一种; DNA合成方法，其中在DNA合成反应中，通过使用DNA聚合酶在上述增强剂的存在下进行反应; 包含上述增强剂的DNA合成反应组合物; 包含两种或更多种各自具有3'→5'核酸外切酶活性的DNA聚合酶的DNA合成反应组合物; 一种DNA合成方法，其中在DNA合成反应中使用两种或更多种具有3'→5'核酸外切酶活性的DNA聚合酶; 用于体外DNA合成的试剂盒，其包含两种或更多种各自具有3'→5'核酸外切酶活性的DNA聚合酶; 和用于体外DNA合成的试剂盒，其中试剂盒包含DNA合成反应增强剂和DNA聚合酶。 根据本发明，与常规DNA合成反应相比，可以以更优异的效果进行DNA合成。

    Method for synthesizing DNA
    35.
    发明授权
    Method for synthesizing DNA 失效
    DNA合成方法

    公开(公告)号：US06673578B1

    公开(公告)日：2004-01-06

    申请号：US09786684

    申请日：2001-05-22

    申请人： Takashi Uemori ,  Yoshimi Sato ,  Mariko Okawa ,  Tomoko Fujita ,  Kazue Miyake ,  Osamu Takeda ,  Hiroaki Sagawa ,  Michio Hagiya ,  Hiroyuki Mukai ,  Kiyozo Asada ,  Ikunoshin Kato

    发明人： Takashi Uemori ,  Yoshimi Sato ,  Mariko Okawa ,  Tomoko Fujita ,  Kazue Miyake ,  Osamu Takeda ,  Hiroaki Sagawa ,  Michio Hagiya ,  Hiroyuki Mukai ,  Kiyozo Asada ,  Ikunoshin Kato

    IPC分类号： C12P1934

    CPC分类号： C12N15/1003 ,  C12Q1/686 ,  C12Q2527/125

    摘要： A DNA synthesis method with a shortened time period required for DNA synthesis by polymerase chain reaction (PCR), characterized in that a DNA polymerase is used in an amount effective for providing more than 10 ng of amplified DNA fragments of about 2 kb per 50 &mgr;l of a reaction mixture, when PCR is carried out under the following conditions (A) and (B): (A) reaction mixture: 50 &mgr;l volume of a reaction mixture comprising DNA polymerase, 1 ng of genomic DNA from Escherichia coli, and 10 pmol each of primers Eco-1 and Eco-2 (nucleotide sequences of the primers Eco-1 and Eco-2 being shown in SEQ ID NOs: 10 and 11 of Sequence Listing, respectively); and having a composition suitable for the DNA polymerase; and (B) reaction conditions: 35 cycles of PCR, wherein one cycle consists of 99° C., 1 second-66° C., 7 seconds; a kit for DNA synthesis usable for the DNA synthesis method; and an article of manufacture of a PCR agent. According to the present invention, the procedures in the genetic engineering studies and industries involved with PCR can be speeded up.

    摘要翻译： 一种DNA合成方法，其通过聚合酶链式反应（PCR）进行DNA合成所需的时间缩短，其特征在于使用DNA聚合酶，其量有效提供超过10ng每50ul约2kb的扩增DNA片段 的反应混合物，当在以下条件（A）和（B）下进行PCR时：（A）反应混合物：将50体积的包含DNA聚合酶的反应混合物，1ng来自大肠杆菌的基因组DNA和10ng 各引物Eco-1和Eco-2（引物Eco-1和Eco-2的核苷酸序列分别示于序列表的SEQ ID NO：10和11中） 并具有适合于DNA聚合酶的组合物; 和（B）反应条件：35个循环的PCR，其中一个循环由99℃，1秒-66℃，7秒; 用于DNA合成方法的DNA合成试剂盒; 以及PCR试剂的制造。 根据本发明，可以加快基因工程研究和涉及PCR的行业的程序。

    Spinning washer for wafers
    36.
    发明授权
    Spinning washer for wafers 有权
    晶圆用旋转垫圈

    公开(公告)号：US06381796B1

    公开(公告)日：2002-05-07

    申请号：US09522626

    申请日：2000-03-10

    申请人： Yoshimi Sato

    发明人： Yoshimi Sato

    IPC分类号： B08B104

    CPC分类号： H01L21/67046 ,  B08B1/04

    摘要： Disclosed is an improved spinning washer comprising: a spinning table for fixedly holding a wafer to be washed; water flushing device; first brush which can be put in coplanar position relative to the lower surface of the wafer round the circumference of the spinning table; and second brush confronting the first brush. The second brush can be put in contact with the upper surface of the wafer in its operative position. The opposite surfaces and circumference side of the wafer can be washed without the fear of breaking the wafer no matter how it may be thin.

    摘要翻译： 公开了一种改进的纺纱清洗机，包括：用于固定地保持要洗涤的晶片的纺丝台; 水冲洗装置; 第一刷可相对于纺丝台周围的晶片的下表面放置在共面位置; 第二个刷子面对第一个刷子。 第二刷可以在其工作位置与晶片的上表面接触。 可以洗涤晶片的相对表面和周边侧，而不用担心破碎晶片，而不管其如何薄。

    Lining for interior and method of producing same
    37.
    发明授权
    Lining for interior and method of producing same 失效
    内衬内衬及其生产方法

    公开(公告)号：US6034009A

    公开(公告)日：2000-03-07

    申请号：US947874

    申请日：1997-10-09

    申请人： Yoshimi Sato

    发明人： Yoshimi Sato

    IPC分类号： B32B5/06 ,  B32B5/26 ,  B32B5/28 ,  B60R13/02 ,  D04H1/485 ,  D04H1/498 ,  D04H1/65

    CPC分类号： B60R13/02 ,  B32B5/28 ,  B60R13/0225 ,  Y10T442/615 ,  Y10T442/619 ,  Y10T442/625 ,  Y10T442/667 ,  Y10T442/668 ,  Y10T442/674

    摘要： A lining for interior comprises a first layer composed of fibers of higher melting point, which are bound by a thermoplastic resin of lower melting point. A second layer is placed on the first layer, which is constructed of a thermoplastic resin of lower melting point. A third layer is placed on the second layer. The third layer is composed of fibers of higher melting point which are bound by a thermoplastic resin of lower melting point. A fourth layer is placed on the third layer, which is constructed of a thermoplastic resin of lower melting point. A fifth layer is placed on the fourth layer, which is constructed of an skin member having higher melting point. The first and third layers are bonded to each other through the second layer and the third and fifth layers are bonded to each other through the fourth layer.

    摘要翻译： 用于内部的衬里包括由较低熔点的热塑性树脂结合的由较高熔点的纤维构成的第一层。 将第二层放置在由较低熔点的热塑性树脂构成的第一层上。 第三层被放置在第二层上。 第三层由具有较低熔点的热塑性树脂结合的较高熔点的纤维组成。 将第四层放置在由较低熔点的热塑性树脂构成的第三层上。 第五层被放置在由具有较高熔点的表皮构件构成的第四层上。 第一层和第三层通过第二层彼此结合，第三层和第五层通过第四层彼此结合。

    Release system for seat occupant in vehicle
    38.
    发明授权
    Release system for seat occupant in vehicle 失效
    汽车座椅乘员释放系统

    公开(公告)号：US4691939A

    公开(公告)日：1987-09-08

    申请号：US790157

    申请日：1985-10-22

    申请人： Yoshimi Sato

    发明人： Yoshimi Sato

    IPC分类号： B60R22/34 ,  B60R22/36

    CPC分类号： B60R22/3416

    摘要： An inventive release system for a seat occupant restrained by a seat belt in a vehicle can be operated both inboard and outboard. The system includes a seat belt retractor locked in response to abrupt deceleration or collision, a mechanism emergency-releasing the retractor after the retractor has been locked, and a manual control including a control terminal exerting a control power for actuating the mechanism and also including inboard and outboard control lever mechanism manually actuated to move the control terminal.

    摘要翻译： 本发明的用于由车辆中的安全带约束的座椅乘客的释放系统可以在内侧和外侧操作。 该系统包括响应于突然减速或碰撞而锁定的安全带卷收器，在牵开器被锁定之后紧急释放牵开器的机构，以及包括控制终端的手动控制，该控制终端施加用于致动该机构的控制功能并且还包括内侧 并且外部控制杆机构手动启动以移动控制终端。

    Seat belt system
    39.
    发明授权
    Seat belt system 失效
    安全带系统

    公开(公告)号：US4354695A

    公开(公告)日：1982-10-19

    申请号：US164275

    申请日：1980-06-30

    申请人： Yoshimi Sato

    发明人： Yoshimi Sato

    IPC分类号： B60R22/06 ,  B60R21/00

    CPC分类号： B60R22/06

    摘要： A seat belt system for an automotive vehicle, includes a shoulder belt the lower end of which is fixed to a floor of the vehicle body and the upper end of which is connected with a slider, a guide rail fixed relative to the vehicle body along its longitudinal direction, the guide rail having its engaging portion to be engaged with an engaging portion of the slider so that the slider is slidably fitted to the guide rail, means for actuating the slider in response to the opening and closing a door of the automotive vehicle, and means for locking the slider in its rearmost position, the engaging portion of the slider facing the side wall of the vehicle body to which the guide rail is fixed, so that the slider can slidably move between the guide rail and the side wall of the vehicle body.

    摘要翻译： 一种用于机动车辆的座椅安全带系统，包括肩带，其下端固定在车体的地板上，并且其上端与滑块连接，相对于车身沿其固定的导轨 所述导轨具有与所述滑块的接合部分接合的接合部分，使得所述滑块可滑动地装配到所述导轨上，用于响应于所述机动车辆的门的打开和关闭而致动所述滑块的装置 ，以及用于将滑块锁定在其最后部位置的装置，滑块的接合部分面向导轨固定到车身侧壁，使得滑块可滑动地在导轨和侧壁之间移动 车体。