公开(公告)号：US20170038334A1

公开(公告)日：2017-02-09

申请号：US15331779

申请日：2016-10-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kristopher BARBEE ,  John DAVIDSON ,  Wolfgang HINZ ,  Shifeng LI ,  James BUSTILLO

IPC: G01N27/447 ,  C12Q1/68 ,  G01N27/414

CPC classification number: G01N27/4473 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00317 ,  B01J2219/00459 ,  B01J2219/00466 ,  B01J2219/00468 ,  B01J2219/005 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00653 ,  B01J2219/00704 ,  B01J2219/00722 ,  C12Q1/6874 ,  G01N27/4145 ,  G01N27/4148 ,  G01N27/44743

Abstract: An apparatus includes a device substrate including an array of sensors. Each sensor of the array of sensors can include a electrode structure disposed at a surface of the device substrate. The apparatus further includes a wall structure overlying the surface of the device substrate and defining an array of wells at least partially corresponding with the array of sensors. The well structure including an electrode layer and an insulative layer.

Abstract translation: 一种装置包括包括传感器阵列的装置基板。 传感器阵列的每个传感器可以包括设置在器件基板的表面处的电极结构。 该装置还包括覆盖在装置基板的表面上的壁结构，并且限定至少部分对应于传感器阵列的一组阱阵列。 阱结构包括电极层和绝缘层。

公开(公告)号：US20170030854A1

公开(公告)日：2017-02-02

申请号：US15222492

申请日：2016-07-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Creighton BUIE ,  John DONOHUE ,  Chiu Tai Andrew WONG ,  James A. BALL ,  Wolfgang HINZ ,  James BUSTILLO ,  Jordan OWENS ,  Shifeng LI

IPC: G01N27/327 ,  C12Q1/68

CPC classification number: C12Q1/6874 ,  G01N27/3276

Abstract: Provided herein is a sensor comprising a substrate having a first reaction region and a second reaction region, a first electrode associated with the first reaction region, a second electrode associated with the second reaction region and a third electrode wherein the third electrode is common to both the first reaction region and the second reaction region.

Abstract translation: 本文提供了一种传感器，其包括具有第一反应区域和第二反应区域的基底，与第一反应区域相关联的第一电极，与第二反应区域相关联的第二电极和第三电极，其中第三电极是共同的 第一反应区和第二反应区。

公开(公告)号：US20160305905A1

公开(公告)日：2016-10-20

申请号：US15194321

申请日：2016-06-27

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Wolfgang HINZ ,  John Matthew MAURO ,  Shifeng LI ,  James BUSTILLO

IPC: G01N27/414 ,  C12Q1/68

CPC classification number: G01N27/4148 ,  C12Q1/6874 ,  G01N27/26 ,  G01N27/414 ,  G01N27/4145 ,  H01L29/78 ,  Y10T436/143333

Abstract: The described embodiments may provide a method of fabricating a chemical detection device. The method may comprise forming a microwell above a CMOS device. The microwell may comprise a bottom surface and sidewalls. The method may further comprise applying a first chemical to be selectively attached to the bottom surface of the microwell, forming a metal oxide layer on the sidewalls of the microwell, and applying a second chemical to be selectively attached to the sidewalls of the microwell. The second chemical may lack an affinity to the first chemical.

公开(公告)号：US20140264469A1

公开(公告)日：2014-09-18

申请号：US14197710

申请日：2014-03-05

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Keith G. FIFE ,  Jordan OWENS ,  Shifeng LI ,  James BUSTILLO

IPC: G01N27/414

CPC classification number: G01N27/4145

Abstract: In one embodiment, a chemical sensor is described. The chemical sensor includes a chemically-sensitive field effect transistor including a floating gate conductor. A material defines an opening overlying the floating gate conductor. The material comprises a conductive element having an inner surface defining a lower portion of a sidewall of the opening. A dielectric is on the conductive element and has an inner surface defining an upper portion of the sidewall.

Abstract translation: 在一个实施例中，描述了化学传感器。 化学传感器包括具有浮栅导体的化学敏感场效应晶体管。 材料限定了覆盖浮栅导体的开口。 该材料包括具有限定开口的侧壁的下部的内表面的导电元件。 电介质位于导电元件上，并具有限定侧壁上部的内表面。

公开(公告)号：US20140113303A1

公开(公告)日：2014-04-24

申请号：US14139647

申请日：2013-12-23

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Wolfgang HINZ ,  John M. MAURO ,  Shifeng LI ,  James BUSTILLO

IPC: G01N27/414 ,  C12Q1/68 ,  G01N27/26

CPC classification number: G01N27/4148 ,  C12Q1/6874 ,  G01N27/26 ,  G01N27/414 ,  G01N27/4145 ,  H01L29/78 ,  Y10T436/143333

Abstract: The described embodiments may provide a method of fabricating a chemical detection device. The method may comprise forming a microwell above a CMOS device. The microwell may comprise a bottom surface and sidewalls. The method may further comprise applying a first chemical to be selectively attached to the bottom surface of the microwell, forming a metal oxide layer on the sidewalls of the microwell, and applying a second chemical to be selectively attached to the sidewalls of the microwell. The second chemical may lack an affinity to the first chemical.

Abstract translation: 所描述的实施例可以提供一种制造化学检测装置的方法。 该方法可以包括在CMOS器件上形成微孔。 微孔可以包括底表面和侧壁。 该方法可以进一步包括施加第一化学物质以选择性地连接到微孔的底表面，在微孔的侧壁上形成金属氧化物层，以及施加第二化学物质以选择性地连接到微孔的侧壁。 第二种化学物质可能与第一种化学物质不具有亲和力。

公开(公告)号：US20130071915A1

公开(公告)日：2013-03-21

申请号：US13675400

申请日：2012-11-13

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： James BUSTILLO ,  Mark MILGREW

IPC: C12Q1/68

CPC classification number: G01N27/4145 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/27 ,  G01N27/414 ,  Y10T29/49002

Abstract: Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.

Abstract translation: 与用于监测化学和/或生物反应的FET阵列相关的方法和装置，例如核酸序列合成反应。 本文提供的一些方法涉及在核酸测序反应期间从释放的氢离子改进信号（以及信噪比）。

公开(公告)号：US20240426775A1

公开(公告)日：2024-12-26

申请号：US18732345

申请日：2024-06-03

Applicant: Life Technologies Corporation

Inventor： James BUSTILLO ,  Wolfgang HINZ ,  Kim JOHNSON ,  Jonathan ROTHBERG

IPC: G01N27/414 ,  C12Q1/6818 ,  C12Q1/6874 ,  G01N33/543 ,  H01L27/088 ,  H01L29/423 ,  H01L29/78

Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.

公开(公告)号：US20240201126A1

公开(公告)日：2024-06-20

申请号：US18536131

申请日：2023-12-11

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： James BUSTILLO ,  Mark J. MILGREW ,  Wolfgang HINZ ,  John LEAMON ,  John DAVIDSON ,  Martin HUBER ,  Antoine M. VAN OIJEN ,  Jonathan ROTHBERG

IPC: G01N27/414 ,  C12Q1/6869 ,  C12Q1/6874 ,  H01L21/82

CPC classification number: G01N27/4145 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/4148 ,  H01L21/82

Abstract: Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.

公开(公告)号：US20240159702A1

公开(公告)日：2024-05-16

申请号：US18353017

申请日：2023-07-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan M. ROTHBERG ,  Wolfgang HINZ ,  Kim L. JOHNSON ,  James BUSTILLO

IPC: G01N27/414 ,  C12Q1/6818 ,  C12Q1/6874 ,  G01N33/543 ,  H01L27/088 ,  H01L29/78

CPC classification number: G01N27/4148 ,  C12Q1/6818 ,  C12Q1/6874 ,  G01N27/4145 ,  G01N33/54373 ,  G01N33/5438 ,  H01L27/088 ,  H01L29/78 ,  H01L29/42324 ,  H01L2924/01006 ,  H01L2924/01013 ,  H01L2924/01015 ,  H01L2924/01073 ,  H01L2924/14 ,  H01L2924/1433 ,  Y10T436/22

Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.

公开(公告)号：US20230152271A1

公开(公告)日：2023-05-18

申请号：US17823696

申请日：2022-08-31

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan M. ROTHBERG ,  Wolfgang HINZ ,  John F. DAVIDSON ,  Antoine M. van OIJEN ,  John LEAMON ,  Martin HUBER ,  Mark James MILGREW ,  James BUSTILLO

IPC: G01N27/414 ,  C12Q1/6874 ,  H01L21/82 ,  C12Q1/6869

CPC classification number: G01N27/4145 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/4148 ,  H01L21/82

Abstract: Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.