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公开(公告)号：US10018617B2
公开(公告)日：2018-07-10
申请号：US15304800
申请日：2015-05-15
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Kyle Gee , Hee Chol Kang , Wenjun Zhou , Bradley Dubbels , Michael Olszowy , Michael O'Grady
IPC: G01N33/50 , C07D471/04 , C07C309/61 , C07K16/28 , C09B11/28
CPC classification number: G01N33/5005 , C07C309/61 , C07C2603/24 , C07D471/04 , C07K16/2812 , C09B11/28 , G01N33/5014 , G01N33/5044
Abstract: Described herein are fluorescent compounds and methods and comprising these compounds. The compounds disclosed herein are carbopyronine reagents that fluoresce in the red portion of the UV/VIS spectrum and provide bright fluorescence intensity, uniform cell staining, and good retention within live cells as well as low toxicity toward cells.
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公开(公告)号：US09645140B2
公开(公告)日：2017-05-09
申请号：US14330727
申请日：2014-07-14
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Brian Agnew , Kyle Gee , Tamara Nyberg
IPC: G01N33/533 , G01N33/532 , G01N33/58 , C12P21/00 , A61K47/48 , G01N33/531 , G01N33/50 , C07K16/00 , G01N33/534
CPC classification number: C12P21/005 , A61K47/549 , A61K47/61 , C07K1/13 , C07K14/47 , C07K16/00 , C07K2317/14 , C07K2317/24 , C07K2317/41 , C12Y302/01096 , G01N33/5005 , G01N33/5008 , G01N33/531 , G01N33/532 , G01N33/533 , G01N33/534 , G01N33/581 , G01N33/582 , G01N33/583 , Y10T436/17
Abstract: Provided in certain embodiments are new methods for forming azido modified biomolecule conjugates of reporter molecules, carrier molecules or solid support. In other embodiments are provided methods for enzymatically labeling a biomolecules with an azide group.
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33.发明申请公开(公告)号：US20170108518A1
公开(公告)日：2017-04-20
申请号：US15340134
申请日：2016-11-01
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Yi-Zhen HU , Aimei Chen , Hee Chol Kang , Kyle Gee , Bhaskar Mandavilli
Abstract: Disclosed herein are compounds, compositions, methods, and kits for detecting reactive oxygen species (ROS) by conventional fluorescence microscopy, fluorescence spectroscopy, flow cytometry, and/or high content imaging. The compounds disclosed herein are novel reduced nucleic acid binding cyanine dyes, which dyes are probes for detecting ROS and measuring oxidative stress in cells either in vitro and/or in vivo. Also described herein are processes for preparing novel reduced dyes, i.e., ROS probes, for use in the disclosed compositions, methods and kits.
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公开(公告)号：US20160356782A1
公开(公告)日：2016-12-08
申请号：US15176872
申请日：2016-06-08
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Aimei CHEN , Kyle Gee , Hee Chol Kang
CPC classification number: G01N33/64 , C07D311/82 , C07D471/06 , C07F5/022 , C07F9/5442 , C07F9/65522 , C09B11/24 , C09K11/06 , C09K2211/10 , C09K2211/1007 , C09K2211/1014 , C09K2211/1022 , C09K2211/1033 , C09K2211/1055 , C09K2211/1088 , G01N21/6428 , G01N21/6486 , G01N33/50 , G01N33/52 , G01N2021/6439 , Y10T436/143333
Abstract: The present disclosure is directed to fluorogenic schiff base-forming dyes capable of detecting analytes containing aldehyde and ketone groups. The dyes contain nucleophilic hydrazinyl appendages and are capable of binding and detecting analytes in situ.
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公开(公告)号：US09440925B2
公开(公告)日：2016-09-13
申请号：US14810766
申请日：2015-07-28
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Kyle Gee , Aimei Chen , Hee Chol Kang
IPC: G01N33/532 , C07K1/13 , C07C309/42 , C07D225/08 , C07D225/02 , C07K16/18 , C07D223/08 , C07D223/26
CPC classification number: C07D225/08 , C07C309/42 , C07C2603/36 , C07D223/08 , C07D223/26 , C07D225/02 , C07K1/13 , C07K16/18 , G01N33/532
Abstract: The present disclosure is directed to a reactive ester agent for conjugating a click-reactive group to a carrier molecule or solid support. The reactive ester agent has the general formula IA, wherein the variables R1, R2, R3, Ra and L are described throughout the application.
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Patent Agency Ranking
公开(公告)号：US09400273B1
公开(公告)日：2016-07-26
申请号：US14801000
申请日：2015-07-16
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Jixiang Liu , Hee Chol Kang , Kyle Gee , Christopher Langsdorf , Jolene Bradford , Gayle Buller
IPC: C12Q1/02 , G01N33/50 , C07D405/14 , C07D405/12
CPC classification number: G01N33/5008 , C07D405/12 , C07D405/14 , G01N33/533 , G01N2430/00
Abstract: Described herein are compounds, methods, and kits for long-term tracking of cell proliferation, differentiation, and/or function. The compounds of the present invention are novel cell-tracking reagents, efficiently excitable with a 405-nm violet laser, that provide bright fluorescence intensity, uniform cell staining, and good retention within cells as well as low toxicity toward cells.
Abstract translation: 本文描述了用于长期跟踪细胞增殖，分化和/或功能的化合物，方法和试剂盒。 本发明的化合物是能够以405nm紫激光激发的新型细胞追踪试剂，能够提供明亮的荧光强度，均匀的细胞染色和细胞内的良好保留以及对细胞的低毒性。
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公开(公告)号：US09372181B2
公开(公告)日：2016-06-21
申请号：US14307445
申请日：2014-06-17
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Kyle Gee , Vladimir Martin
IPC: G01N23/00 , G01N33/20 , C07C229/18 , C07D221/14 , C07D265/38 , C07D311/82 , C09B5/60 , C09B5/62 , C09B11/06 , G01N33/84 , C07D209/14 , C07D311/90 , C07D413/04
CPC classification number: G01N33/84 , C07C229/18 , C07C311/44 , C07D209/14 , C07D221/14 , C07D265/38 , C07D311/82 , C07D311/90 , C07D413/04 , C07K14/195 , C07K14/43595 , C09B5/60 , C09B5/62 , C09B11/06 , C09B11/08 , C09B19/00 , C09B19/005 , C09B57/08 , C09K11/06 , C09K2211/1014 , G01N33/20 , G01N2458/00
Abstract: The present invention provides fluorogenic compounds for the detection of target metal ions wherein the compounds exhibit a Stokes shift greater than 50 nm and the detectable signal is modulated by photoinduced electron transfer (PET). The present compounds consist of three functional elements, the ion sensing moiety (chelating moiety), the reporter moiety (fluorophore or fluorescent protein) and spacer or linker between the sensing and reporter moieties of the present compound that allows for PET upon binding of a metal ion and excitation by an appropriate wavelength.
Abstract translation: 本发明提供用于检测靶金属离子的荧光化合物，其中化合物表现出大于50nm的斯托克斯位移，并且可检测信号通过光诱导电子转移（PET）调节。 本发明化合物由三种功能元件组成，即离子感测部分（螯合部分），报道部分（荧光团或荧光蛋白）以及本发明化合物的感测和报道部分之间的间隔物或接头，其允许在结合金属时的PET 通过适当的波长离子和激发。
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公开(公告)号：US20140363824A1
公开(公告)日：2014-12-11
申请号：US14307445
申请日：2014-06-17
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Kyle Gee , Vladimir Martin
IPC: G01N33/20
CPC classification number: G01N33/84 , C07C229/18 , C07C311/44 , C07D209/14 , C07D221/14 , C07D265/38 , C07D311/82 , C07D311/90 , C07D413/04 , C07K14/195 , C07K14/43595 , C09B5/60 , C09B5/62 , C09B11/06 , C09B11/08 , C09B19/00 , C09B19/005 , C09B57/08 , C09K11/06 , C09K2211/1014 , G01N33/20 , G01N2458/00
Abstract: The present invention provides fluorogenic compounds for the detection of target metal ions wherein the compounds exhibit a Stokes shift greater than 50 nm and the detectable signal is modulated by photoinduced electron transfer (PET). The present compounds consist of three functional elements, the ion sensing moiety (chelating moiety), the reporter moiety (fluorophore or fluorescent protein) and spacer or linker between the sensing and reporter moieties of the present compound that allows for PET upon binding of a metal ion and excitation by an appropriate wavelength.
Abstract translation: 本发明提供用于检测靶金属离子的荧光化合物，其中化合物表现出大于50nm的斯托克斯位移，并且可检测信号通过光诱导电子转移（PET）调节。 本发明化合物由三种功能元件组成，即离子感测部分（螯合部分），报道部分（荧光团或荧光蛋白）以及本发明化合物的感测和报道部分之间的间隔物或接头，其允许在结合金属时的PET 通过适当的波长离子和激发。
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公开(公告)号：US20240042062A1
公开(公告)日：2024-02-08
申请号：US18380388
申请日：2023-10-16
Applicant: Life Technologies Corporation , Pierce Biotechnology, Inc.
Inventor： Surbhi Desai , Marie Christine Nlend , Kyle Gee , Matthew Baker , Robert Aggeler , Scott Sweeney , Aleksey Rukavishnikov , Shih-Jung Huang
CPC classification number: A61K49/0015 , G01N33/582 , A61K47/10 , A61K47/18 , A61K49/0058 , G01N33/533 , G01N33/68
Abstract: This disclosure relates to the field of fluorescent dyes, and in particular, compositions and methods for increasing fluorescent signals and the reduction of fluorescent quenching.
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公开(公告)号：US11067580B2
公开(公告)日：2021-07-20
申请号：US16838004
申请日：2020-04-01
Applicant: Life Technologies Corporation
Inventor： Kyle Gee , Upinder Singh , Scott Grecian , Scott Clarke
IPC: C07D213/74 , G01N33/58 , G01N33/533 , C07D213/79 , C07D213/81 , C07D215/12 , C07D401/04 , C07D235/14 , C07D213/53 , C07D213/68 , C07D213/78 , C09B23/06 , C09B23/08 , C09B11/24 , C07D401/14 , C07K14/575
Abstract: Low-copper click chemistry, 1.3-dipolar cycloadditions, and Staudinger ligations for modifying biomolecules is provided. Compositions, methods, and kits relating to low-copper click chemistry, 1.3-dipolar cycloadditions, and Staudinger ligations are also provided.
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