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    METHOD FOR PREPARING MODIFIED POLYPEPTIDES
    31.
    发明申请
    METHOD FOR PREPARING MODIFIED POLYPEPTIDES 审中-公开
    制备改性多糖的方法

    公开(公告)号：US20120107852A1

    公开(公告)日：2012-05-03

    申请号：US13272681

    申请日：2011-10-13

    申请人： Torben Halkier ,  Anders Hjelholt Pedersen ,  Jens Sigurd Okkels ,  Kim Vilbour Andersen

    发明人： Torben Halkier ,  Anders Hjelholt Pedersen ,  Jens Sigurd Okkels ,  Kim Vilbour Andersen

    IPC分类号： C12Q1/48

    CPC分类号： C12N15/1058 ,  C07K1/107 ,  C07K14/31 ,  C07K19/00 ,  C12N15/1034

    摘要： Methods for producing polypeptide with altered immunogenicity or improved stability properties are disclosed. The methods involve a) expressing a diversified population of nucleotide sequences encoding a polypeptide of interest, b) screening the polypeptides expressed in step a) for function, immunogenicity and/or stability, c) selecting functional polypeptides having altered immunogenicity and/or increased stability, e.g. functional in vivo half-life as compared to the polypeptide of interest, and d) optionally subjecting the nucleotide sequence encoding the polypeptide selected in step c) to one or more repeated cycles of steps a)-c). In a further step the expressed polypeptides of step a) or c) can be conjugated to at least one non-polypeptide moiety.

    摘要翻译： 公开了产生具有改变的免疫原性或改善的稳定性的多肽的方法。 所述方法包括：a）表达编码感兴趣多肽的核苷酸序列的多样化群体，b）筛选功能，免疫原性和/或稳定性在步骤a）中表达的多肽，c）选择具有改变的免疫原性和/或增加稳定性的功能性多肽 ，例如 与目的多肽相比具有功能的体内半衰期，和d）任选地将编码步骤c）中选择的多肽的核苷酸序列经历步骤a）-c）的一个或多个重复循环。 在另一步骤中，步骤a）或c）的表达多肽可以与至少一个非多肽部分缀合。

    RANK LIGAND-BINDING POLYPEPTIDES
    32.
    发明申请
    RANK LIGAND-BINDING POLYPEPTIDES 审中-公开
    RANK配体结合多肽

    公开(公告)号：US20090017499A1

    公开(公告)日：2009-01-15

    申请号：US11942684

    申请日：2007-11-19

    申请人： Jesper Mortensen Haaning ,  Torben Halkier

    发明人： Jesper Mortensen Haaning ,  Torben Halkier

    IPC分类号： C12P21/02 ,  C07K14/47 ,  C12N15/62

    CPC分类号： C07K14/70578 ,  C07K2319/00

    摘要： The present invention relates to a polypeptide having an amino acid sequence that differs from and is at least 70% identical to the amino acid sequence of hRANK, and which has a binding affinity to RANKL that is at least as high as the binding affinity of hRANK to RANKL, as determined by the functional competition assay described herein.

    摘要翻译： 本发明涉及具有与hRANK的氨基酸序列不同且至少70％相同的氨基酸序列的多肽，并且其与RANKL的结合亲和力至少与hRANK的结合亲和力一样高 通过本文所述的功能竞争测定法确定的RANKL。

    Method for down-regulating GDF-8 activity using immunogenic GDF-8 analogues
    34.
    发明授权
    Method for down-regulating GDF-8 activity using immunogenic GDF-8 analogues 失效
    使用免疫原性GDF-8类似物下调GDF-8活性的方法

    公开(公告)号：US07070784B1

    公开(公告)日：2006-07-04

    申请号：US10031342

    申请日：2000-07-20

    申请人： Torben Halkier ,  Søren Mouritsen ,  Steen Klysner

    发明人： Torben Halkier ,  Søren Mouritsen ,  Steen Klysner

    IPC分类号： A61K39/00 ,  C07K14/00

    CPC分类号： C07K14/475 ,  A61K38/00 ,  A61K39/00 ,  A61K39/0005 ,  A61K48/00 ,  A61K2039/523 ,  A61K2039/53 ,  A61K2039/55516 ,  A61K2039/55522 ,  C07K2319/00

    摘要： Disclosed are novel methods for increasing muscle mass by means of immunization against Growth Differentiation Factor 8 (GDF-8, myostatin). Immunization is preferably effected by administration of analogues of GDF-8 which are capable of inducing antibody production against homologous GDF-8. Especially preferred as an immunogen is homologous GDF-8 which has been modified by introduction of one single or a few foreign, immunodominant and promiscuous T-cell epitopes while substantially preserving the tertiary structure of the homologous GDF-8. Also disclosed are nucleic acid vaccination against GDF-8 and vaccination using live vaccines as well as methods and means useful for the vaccination. Such methods and means include methods for identification of useful immunogenic GDF-8 analogues, methods for the preparation of analogues and pharmaceutical formulations, as well as nucleic acid fragments, vectors, transformed cells, polypeptides and pharmaceutical formulations.

    摘要翻译： 公开了通过免疫接种生长分化因子8（GDF-8，肌生成抑制素）来增加肌肉质量的新方法。 免疫优选通过施用能够诱导针对同源GDF-8的抗体产生的GDF-8的类似物来实现。 特别优选的是作为免疫原的同源GDF-8，其通过引入一个或多个外来的免疫显性和混杂的T细胞表位而被修饰，同时基本上保留了同源的GDF-8的三级结构。 还公开了针对GDF-8的核酸疫苗接种和使用活疫苗的疫苗接种以及用于疫苗接种的方法和装置。 这些方法和手段包括鉴定有用的免疫原性GDF-8类似物的方法，制备类似物和药物制剂的方法，以及核酸片段，载体，转化细胞，多肽和药物制剂。