公开(公告)号：US20070264666A1

公开(公告)日：2007-11-15

申请号：US11828572

申请日：2007-07-26

申请人： Timothy Woudenberg ,  Robert Jones ,  Kenneth Livak

发明人： Timothy Woudenberg ,  Robert Jones ,  Kenneth Livak

IPC分类号： C12Q1/68 ,  C07H21/00

CPC分类号： B01L3/50851 ,  B01L3/50853 ,  B01L3/50857 ,  B01L2200/0642 ,  B01L2300/044 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2400/0409 ,  B01L2400/0487 ,  C12Q1/6837

摘要： A method for performing PCR on a liquid sample comprising a plurality of polynucleotide targets, wherein each polynucleotide target is present at very low concentration within the sample. The method comprises applying PCR reactants to the surface of a substrate to produce a plurality of reaction spots on the surface of the substrate; loading the liquid sample and a PCR reagent mixture onto the reaction spots; forming a sealed reaction chamber, having a volume of less than about 20 nanoliters, over each of the reaction spots; and amplifying the sample.

摘要翻译： 在包含多个多核苷酸靶标的液体样品上进行PCR的方法，其中每个多核苷酸靶标以非常低的浓度存在于样品内。 该方法包括将PCR反应物施加到基底的表面以在基底的表面上产生多个反应点; 将液体样品和PCR试剂混合物加载到反应点上; 在每个反应点上形成体积小于约20纳升的密封反应室; 并放大样品。

公开(公告)号：US20070111300A1

公开(公告)日：2007-05-17

申请号：US11651321

申请日：2007-01-09

申请人： Timothy Woudenberg ,  Michael Albin ,  Reid Kowallis ,  Yefim Raysberg ,  Robert Ragusa ,  Emily Winn-Deen

发明人： Timothy Woudenberg ,  Michael Albin ,  Reid Kowallis ,  Yefim Raysberg ,  Robert Ragusa ,  Emily Winn-Deen

IPC分类号： C12M1/34

CPC分类号： C12Q1/6816 ,  B01J2219/00317 ,  B01J2219/00599 ,  B01J2219/00722 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/50851 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0642 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/1827 ,  B01L2400/049 ,  C12Q1/08 ,  C40B40/06 ,  C40B50/08 ,  C40B60/14 ,  G01N21/6452 ,  G01N2035/00148 ,  Y10S436/805 ,  Y10S436/807 ,  Y10S436/808 ,  Y10S436/809 ,  Y10T436/143333 ,  Y10T436/25 ,  C12Q2565/631

摘要： The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. The device includes a substrate which defines a sample-distribution network having (i) a sample inlet, (ii) one or more detection chambers, and (iii) channel means providing a dead-end fluid connection between each of the chambers and the inlet. Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.

摘要翻译： 本发明涉及一种用于同时测试样品的一种或多种选择的分析物的存在，不存在和/或量的方法和装置。 本发明在一个方面包括用于检测或定量液体样品中的多种不同分析物的装置。 该装置包括限定样品分配网络的基板，其具有（i）样品入口，（ii）一个或多个检测室，以及（iii）通道装置，其在每个室之间提供死端流体连接，并且入口 。 每个室可以包括有效地与可能存在于样品中的选定分析物反应的分析物特异性试剂和用于检测信号的检测装置。 还公开了利用该装置的方法。

公开(公告)号：US20060269641A1

公开(公告)日：2006-11-30

申请号：US11433892

申请日：2006-05-12

申请人： John Atwood ,  Albert Mossa ,  Lisa Goven ,  Fenton Williams ,  Timothy Woudenberg ,  Marcel Margulies ,  Robert Ragusa ,  Richard Leath ,  Clive Miles

发明人： John Atwood ,  Albert Mossa ,  Lisa Goven ,  Fenton Williams ,  Timothy Woudenberg ,  Marcel Margulies ,  Robert Ragusa ,  Richard Leath ,  Clive Miles

IPC分类号： B01L9/00

CPC分类号： B01L3/50851 ,  B01L3/50853 ,  B01L7/00 ,  B01L7/52 ,  B01L2200/147 ,  B01L2300/042 ,  B01L2300/046 ,  B01L2300/0829 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/185 ,  G05D23/1917 ,  Y10S435/809

摘要： A thermal cycler for automatic performance of the polymerase chain reaction is provided. The thermal cycler comprises a heater control that provides close temperature control of the reaction.

公开(公告)号：US20060183151A1

公开(公告)日：2006-08-17

申请号：US11400611

申请日：2006-04-07

申请人： Timothy Woudenberg ,  Michael Albin ,  Reid Kowallis ,  Yefim Raysberg ,  Robert Ragusa ,  Emily Winn-Deen

发明人： Timothy Woudenberg ,  Michael Albin ,  Reid Kowallis ,  Yefim Raysberg ,  Robert Ragusa ,  Emily Winn-Deen

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6816 ,  B01J2219/00317 ,  B01J2219/00599 ,  B01J2219/00722 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/50851 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0642 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/1827 ,  B01L2400/049 ,  C12Q1/08 ,  C40B40/06 ,  C40B50/08 ,  C40B60/14 ,  G01N21/6452 ,  G01N2035/00148 ,  Y10S436/805 ,  Y10S436/807 ,  Y10S436/808 ,  Y10S436/809 ,  Y10T436/143333 ,  Y10T436/25 ,  C12Q2565/631

摘要： The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more of a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.

公开(公告)号：US20050158781A1

公开(公告)日：2005-07-21

申请号：US11029968

申请日：2005-01-05

申请人： Timothy Woudenberg ,  Michael Albin ,  Reid Kowallis ,  Yefim Raysberg ,  Robert Raqusa ,  Emily Winn-Deen

发明人： Timothy Woudenberg ,  Michael Albin ,  Reid Kowallis ,  Yefim Raysberg ,  Robert Raqusa ,  Emily Winn-Deen

IPC分类号： B01L3/00 ,  C12Q1/08 ,  C40B40/06 ,  C40B50/08 ,  C40B60/14 ,  G01N35/00 ,  C12Q1/68 ,  C12M1/34

CPC分类号： C12Q1/6816 ,  B01J2219/00317 ,  B01J2219/00599 ,  B01J2219/00722 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/50851 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0642 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/1827 ,  B01L2400/049 ,  C12Q1/08 ,  C40B40/06 ,  C40B50/08 ,  C40B60/14 ,  G01N21/6452 ,  G01N2035/00148 ,  Y10S436/805 ,  Y10S436/807 ,  Y10S436/808 ,  Y10S436/809 ,  Y10T436/143333 ,  Y10T436/25 ,  C12Q2565/631

摘要： The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more of a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.

摘要翻译： 本发明涉及一种用于同时测试样品中多种所选分析物中一种或多种的存在，不存在和/或量的方法和装置。 本发明在一个方面包括用于检测或定量液体样品中的多种不同分析物的装置。 每个室可以包括有效地与可能存在于样品中的选定分析物反应的分析物特异性试剂和用于检测信号的检测装置。 还公开了利用该装置的方法。

公开(公告)号：US20050084957A1

公开(公告)日：2005-04-21

申请号：US10929961

申请日：2004-08-30

申请人： John Atwood ,  Susan Stone ,  Judith Atwood ,  Albert Mossa ,  Lisa Goven ,  Fenton Williams ,  Timothy Woudenberg ,  Marcel Margulies ,  Robert Ragusa ,  Richard Leath ,  Clive Miles

发明人： John Atwood ,  Susan Stone ,  Judith Atwood ,  Albert Mossa ,  Lisa Goven ,  Fenton Williams ,  Timothy Woudenberg ,  Marcel Margulies ,  Robert Ragusa ,  Richard Leath ,  Clive Miles

IPC分类号： B01L3/00 ,  B01L7/00 ,  G05D23/19 ,  C12M1/24

CPC分类号： B01L3/50851 ,  B01L3/50853 ,  B01L7/00 ,  B01L7/52 ,  B01L2200/147 ,  B01L2300/042 ,  B01L2300/046 ,  B01L2300/0829 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/185 ,  G05D23/1917 ,  Y10S435/809

摘要： A sample tube including a cylindrical part and a conical part is described. The cylindrical part can include, a first wall portion having a first wall portion thickness, a second wall portion having a second wall portion thickness, and a shoulder including a planar first surface and a conically beveled second surface disposed between the first wall portion and the second wall portion. The first wall portion can be disposed closer to the planar first surface than the conically beveled second surface. The conical part can be shaped as a frustrum of a cone having a conical part wall thickness. The frustrum can include a wider-end and a narrower-end. The wider-end of the frustrum can be in contact with the second wall portion of the cylindrical part. At least one of the first wall portion thickness and the second wall portion thickness can be greater than the conical part wall thickness.

摘要翻译： 描述了包括圆柱形部分和圆锥形部分的样品管。 圆柱形部分可以包括具有第一壁部分厚度的第一壁部分，具有第二壁部分厚度的第二壁部分和包括平面第一表面和设置在第一壁部分和第二壁部分之间的锥形斜面第二表面的肩部 第二壁部。 第一壁部分可以比锥形倾斜的第二表面更靠近平面的第一表面设置。 锥形部分可以形成为具有圆锥形部分壁厚度的圆锥体。 风险可以包括更广泛的和更窄的一端。 膛孔的较宽端可与圆柱形部分的第二壁部分接触。 第一壁部分厚度和第二壁部分厚度中的至少一个可以大于锥形部分壁厚度。

公开(公告)号：US20120261007A1

公开(公告)日：2012-10-18

申请号：US13295028

申请日：2011-11-11

申请人： Martin Pieprzyk ,  Geoff Facer ,  Timothy Woudenberg ,  Brian Fowler

发明人： Martin Pieprzyk ,  Geoff Facer ,  Timothy Woudenberg ,  Brian Fowler

IPC分类号： F16K31/12

CPC分类号： G01N1/28 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/16 ,  B01L2300/0867 ,  B01L2300/0874 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0655 ,  B33Y80/00 ,  C12Q1/686 ,  G01N21/05 ,  G01N21/6452 ,  G01N2021/0346 ,  Y10T137/7793 ,  Y10T436/2575

摘要： Embodiments of the present invention provide improved microfluidic devices and related apparatus, systems, and methods. Methods are provided for reducing mixing times during use of microfluidic devices. Microfluidic devices and related methods of manufacturing are provided with increased manufacturing yield rates. Improved apparatus and related systems are provided for supplying controlled pressure to microfluidic devices. Methods and related microfluidic devices are provided for reducing dehydration of microfluidic devices during use. Microfluidic devices and related methods are provided with improved sample to reagent mixture ratio control. Microfluidic devices and systems are provided with improved resistance to compression fixture pressure induced failures. Methods and systems for conducting temperature controlled reactions using microfluidic devices are provided that reduce condensation levels within the microfluidic device. Methods and systems are provided for improved fluorescent imaging of microfluidic devices.

摘要翻译： 本发明的实施例提供了改进的微流体装置和相关装置，系统和方法。 提供了减少使用微流体装置期间的混合时间的方法。 微流控装置和相关的制造方法具有提高的制造成品率。 提供改进的装置和相关系统用于向微流体装置提供受控的压力。 提供了方法和相关的微流体装置，用于在使用过程中减少微流体装置的脱水。 微流控装置和相关方法提供了改进的样品与试剂混合比控制。 微流体装置和系统具有改进的抗压缩夹具压力引起的故障的阻力。 提供了使用微流体装置进行温度控制反应的方法和系统，其减少微流体装置内的冷凝水平。 提供了用于改进微流体装置的荧光成像的方法和系统。

公开(公告)号：US20100261158A1

公开(公告)日：2010-10-14

申请号：US11423403

申请日：2006-06-09

申请人： Eric S. Nordman ,  Mark F. Oldham ,  Timothy Woudenberg

发明人： Eric S. Nordman ,  Mark F. Oldham ,  Timothy Woudenberg

IPC分类号： C12Q1/68 ,  C12M1/34 ,  C40B60/14 ,  C40B60/00 ,  C02F1/461

CPC分类号： C12Q1/6874 ,  C12Q1/6869 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/648 ,  G01N21/6486 ,  G01N2021/6441 ,  C12Q2563/107

摘要： The present disclosure provides apparatus, systems and method for detecting separately and substantially simultaneously light emissions from a plurality of localized light-emitting analytes. A system according to exemplary embodiments of the present disclosure comprises a sample holder having structures formed thereon for spatially separating and constraining a plurality of light-emitting analytes each having a single nucleic acid molecule or a single nucleic acid polymerizing enzyme, a light source configured to illuminate the sample holder, an optical assembly configured to collect and detect separately and substantially simultaneously light emissions associated with the plurality of light emitting analytes. The system may further include a computer system configured to analyze the light emissions to determine the structures or properties of a target nucleic acid molecule associated with each analyte.

摘要翻译： 本公开提供了用于单独和基本同时地检测来自多个局部发光分析物的光发射的装置，系统和方法。 根据本公开的示例性实施例的系统包括具有形成在其上的结构的样本保持器，用于空间分离和约束各自具有单个核酸分子或单个核酸聚合酶的多个发光分析物，光源被配置为 照亮样品架，光学组件被配置成分别收集和检测与多个发光分析物相关联的基本上同时的发光。 该系统还可以包括被配置为分析光发射以确定与每个分析物相关联的靶核酸分子的结构或性质的计算机系统。

公开(公告)号：US20100230613A1

公开(公告)日：2010-09-16

申请号：US12688462

申请日：2010-01-15

申请人： Martin Pieprzyk ,  Geoff Facer ,  Timothy Woudenberg ,  Brian Fowler

发明人： Martin Pieprzyk ,  Geoff Facer ,  Timothy Woudenberg ,  Brian Fowler

IPC分类号： G01J1/58

CPC分类号： G01N1/28 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/16 ,  B01L2300/0867 ,  B01L2300/0874 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0655 ,  B33Y80/00 ,  C12Q1/686 ,  G01N21/05 ,  G01N21/6452 ,  G01N2021/0346 ,  Y10T137/7793 ,  Y10T436/2575

摘要： Embodiments of the present invention provide improved microfluidic devices and related apparatus, systems, and methods. Methods are provided for reducing mixing times during use of microfluidic devices. Microfluidic devices and related methods of manufacturing are provided with increased manufacturing yield rates. Improved apparatus and related systems are provided for supplying controlled pressure to microfluidic devices. Methods and related microfluidic devices are provided for reducing dehydration of microfluidic devices during use. Microfluidic devices and related methods are provided with improved sample to reagent mixture ratio control. Microfluidic devices and systems are provided with improved resistance to compression fixture pressure induced failures. Methods and systems for conducting temperature controlled reactions using microfluidic devices are provided that reduce condensation levels within the microfluidic device. Methods and systems are provided for improved fluorescent imaging of microfluidic devices.

摘要翻译： 本发明的实施例提供了改进的微流体装置和相关装置，系统和方法。 提供了减少使用微流体装置期间的混合时间的方法。 微流控装置和相关的制造方法具有提高的制造产率。 提供改进的装置和相关系统用于向微流体装置提供受控的压力。 提供了方法和相关的微流体装置，用于在使用过程中减少微流体装置的脱水。 微流控装置和相关方法提供了改进的样品与试剂混合比控制。 微流体装置和系统具有改进的抗压缩夹具压力引起的故障的阻力。 提供了使用微流体装置进行温度控制反应的方法和系统，其减少微流体装置内的冷凝水平。 提供了用于改进微流体装置的荧光成像的方法和系统。

公开(公告)号：US20100112683A2

公开(公告)日：2010-05-06

申请号：US11999179

申请日：2007-12-04

申请人： John Atwood ,  Albert Mossa ,  Lisa Goven ,  Fenton Williams ,  Timothy Woudenberg ,  Marcel Margulies ,  Robert Ragusa ,  Richard Leath ,  Clive Miles

发明人： John Atwood ,  Albert Mossa ,  Lisa Goven ,  Fenton Williams ,  Timothy Woudenberg ,  Marcel Margulies ,  Robert Ragusa ,  Richard Leath ,  Clive Miles

IPC分类号： C12M1/00

CPC分类号： B01L3/50851 ,  B01L3/50853 ,  B01L7/00 ,  B01L7/52 ,  B01L2200/147 ,  B01L2300/042 ,  B01L2300/046 ,  B01L2300/0829 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/185 ,  G05D23/1917 ,  Y10S435/809

摘要： A thermal cycler for automatic performance of the polymerase chain reaction is provided. The thermal cycler comprises a heater control that provides close temperature control of the reaction.

摘要翻译： 提供了用于自动执行聚合酶链式反应的热循环仪。 热循环仪包括提供对反应的温度控制的加热器控制。