公开(公告)号：USD733914S1

公开(公告)日：2015-07-07

申请号：US29420890

申请日：2012-05-14

Applicant: Axl Neurauter ,  Berit Reed ,  Karoline Schjetne ,  Jeremy (“Jez”) Mundy ,  Tine Hildisch

Designer： Axl Neurauter ,  Berit Reed ,  Karoline Schjetne ,  Jeremy (“Jez”) Mundy ,  Tine Hildisch

公开(公告)号：US20140231697A1

公开(公告)日：2014-08-21

申请号：US14228158

申请日：2014-03-27

Applicant: LIFE TECHNOLOGIES AS

Inventor： Ellen WENG ,  Geir FONNUM ,  Grete MODAHL ,  Astrid MOLTEBERG ,  Erling FINNE ,  Torkel STENE

IPC: C09K11/06 ,  H01F1/01 ,  C09K11/02 ,  H01F1/00

CPC classification number: C09K11/06 ,  B82Y25/00 ,  B82Y40/00 ,  C08F220/18 ,  C09K11/025 ,  G01N33/5434 ,  H01F1/0018 ,  H01F1/01 ,  H01F1/26 ,  H01F41/00 ,  Y10T428/2991 ,  Y10T428/2998

Abstract: A coated magnetic particle comprising an optionally porous magnetic polymer particle of a matrix polymer, said polymer particle having on a surface and/or in the pores thereof superparamagnetic crystals, said coated particle having a coat formed of a coating polymer, wherein said coated magnetic particle is essentially non-autofluorescent.

Abstract translation: 一种涂覆的磁性颗粒，其包含基质聚合物的任选多孔磁性聚合物颗粒，所述聚合物颗粒在表面上和/或其孔中具有超顺磁性晶体，所述涂覆颗粒具有由涂覆聚合物形成的涂层，其中所述涂覆的磁性颗粒 本质上是非自发荧光。

公开(公告)号：US08691969B2

公开(公告)日：2014-04-08

申请号：US13160428

申请日：2011-06-14

Applicant: Arne Helge Deggerdal ,  Frank Larsen

Inventor： Arne Helge Deggerdal ,  Frank Larsen

IPC: C07H21/00

CPC classification number: C12Q1/6806 ,  C12N15/1006 ,  C12N15/1013

Abstract: The present invention provides a method of isolating nucleic acid from a sample, said method comprising contacting said sample with a detergent and a solid support, whereby soluble nucleic acid in said sample is bound to the support, and separating said support with bound nucleic acid from the sample. Where the method of the invention is used to isolate DNA, it may conveniently be couple with a further step to isolate RNA from the same sample.

Abstract translation: 本发明提供了从样品中分离核酸的方法，所述方法包括使所述样品与洗涤剂和固体支持物接触，由此使所述样品中的可溶性核酸与载体结合，并将所述载体与结合的核酸从 例子。 当本发明的方法用于分离DNA时，可以方便地与进一步的步骤相结合以从同一样品中分离RNA。

公开(公告)号：US20140073738A1

公开(公告)日：2014-03-13

申请号：US14082336

申请日：2013-11-18

Applicant: LIFE TECHNOLOGIES AS ,  LIFE TECHNOLOGIES CORPORATION

Inventor： Geir FONNUM ,  Grete I. MODAHL ,  Nini H. KJUS ,  Astrid E. MOLTEBERG ,  Diem TRAN ,  Jo AASERUD ,  Talha M. GOKMEN ,  Steven M. MENCHEN ,  Carl FULLER ,  Luisa ANDRUZZI ,  Wolfgang HINZ

IPC: C08F130/08 ,  C08F8/32

CPC classification number: C08F130/08 ,  C07F7/1804 ,  C07F7/188 ,  C08F2/20 ,  C08F2/26 ,  C08F6/24 ,  C08F8/12 ,  C08F8/32 ,  C08F8/34 ,  C08F220/58 ,  C08F222/385 ,  C08F230/08 ,  C08F2800/20 ,  C08J9/20 ,  C08J2343/04 ,  C12P19/34 ,  C12Q1/686 ,  C12Q1/6874 ,  G01N27/4145 ,  Y10T428/2982 ,  C08F257/02 ,  C08F2222/1013 ,  C08F212/36 ,  C08F220/60 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/543

Abstract: A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.

Abstract translation: 形成颗粒的方法包括在水性悬浮液中的分散相中聚合具有疏水保护基团的亲水性单体的多个聚合单元，从而形成包含多个疏水保护基团的聚合物颗粒。 该方法还包括将聚合物颗粒转化为亲水性颗粒。

公开(公告)号：US20230322996A1

公开(公告)日：2023-10-12

申请号：US18053066

申请日：2022-11-07

Applicant: LIFE TECHNOLOGIES AS

Inventor： Huey Wen OOI ,  Pontus LUNDBERG ,  Maxi-Lu BOESCHEN ,  Hilde ALMAASBAK ,  Tuva Holt HERENG ,  Tor Espen STAV-NORAAS ,  Ole LANDSVERK ,  Geir FONNUM

IPC: C08F283/04

CPC classification number: C08F283/04

Abstract: The invention relates to polymer-interaction molecule (e.g., antibodies) conjugates and uses of such conjugates for delivery of interaction molecules to cells. The invention also relates uses polymer-interaction molecule (e.g., antibodies) conjugates for eliciting cellular responses (e.g., induction of cell proliferation).

公开(公告)号：US20230295605A1

公开(公告)日：2023-09-21

申请号：US18069171

申请日：2022-12-20

Applicant: LIFE TECHNOLOGIES AS

Inventor： Laure JOBERT

IPC: C12N15/10

CPC classification number: C12N15/1013

Abstract: This invention relates to methods and compositions for coupling nucleic acid to a functionalized surface or support. In particular, the present invention provides an improved process for coupling aminated nucleic acid to a support functionalized with carboxylic acid groups, wherein the coupling reaction is conducted in the presence of an organic solvent. The invention further relates to compositions and kits for performing the coupling reaction and uses of nucleic acid-loaded supports for various applications.

公开(公告)号：US11130833B2

公开(公告)日：2021-09-28

申请号：US17066326

申请日：2020-10-08

Applicant: Life Technologies AS

Inventor： Geir Fonnum ,  Nils Pontus Lundberg ,  Muhammed Talha Gokmen ,  Synne Larsen ,  Wilhelm Glomm ,  Heidi Johnsen ,  Ruth Schmid ,  Lars Kilaas

IPC: C08F265/10 ,  C08F120/54 ,  C08F265/06

Abstract: This invention relates to monodisperse cross-linked hydrogel polymer particles comprising a polymer formed from (a) a hydrophilic vinylic monomer; and (b) a crosslinker comprising at least two vinyl groups. The invention also relates to monodisperse seed particles with a Z-average diameter of from 100 nm to 1500 nm that comprise a plurality of non-crosslinked oligomers of poly N,N-dimethylacrylamide. Also provided are methods of forming the monodisperse cross-linked hydrogel polymer particles and monodisperse seed particles.

公开(公告)号：US10676790B2

公开(公告)日：2020-06-09

申请号：US16206957

申请日：2018-11-30

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Geir Fonnum ,  Steven M. Menchen ,  Alfred Lui ,  Guobing Xiang ,  Synne Larsen

IPC: C12Q1/68 ,  C12Q1/6876 ,  C12Q1/6874 ,  C12Q1/6869 ,  C08J7/14 ,  C12Q1/6853

Abstract: A polymer substrate, such as a polymer coating or a polymer hydrogel network, includes carboxyl moieties that can be used as conjugation sites to which receptor or analyte molecules can be attached. In an example, the polymer substrate includes a polyacrylamide polymer network having alkanoic acid moieties or derivatives thereof, which can react with carboxyl activating compounds to provide an activated alkanoate moieties on the polyacrylamide network. Amine-terminated nucleic acids can react with the activated alkanoate moieties to capture the nucleic acid to the polymer network through an alkylamide moiety.

公开(公告)号：US10144968B2

公开(公告)日：2018-12-04

申请号：US15200588

申请日：2016-07-01

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Geir Fonnum ,  Steven M. Menchen ,  Alfred Lui ,  Guobing Xiang ,  Synne Larsen

IPC: C12Q1/68 ,  C12Q1/6876 ,  C12Q1/6869 ,  C12Q1/6874

Abstract: A polymer substrate, such as a polymer coating or a polymer hydrogel network, includes carboxyl moieties that can be used as conjugation sites to which receptor or analyte molecules can be attached. In an example, the polymer substrate includes a polyacrylamide polymer network having alkanoic acid moieties or derivatives thereof, which can react with carboxyl activating compounds to provide an activated alkanoate moieties on the polyacrylamide network. Amine-terminated nucleic acids can react with the activated alkanoate moieties to capture the nucleic acid to the polymer network through an alkylamide moiety.

公开(公告)号：US10017586B2

公开(公告)日：2018-07-10

申请号：US15453750

申请日：2017-03-08

Applicant: LIFE TECHNOLOGIES AS

Inventor： Grete Modahl ,  Geir Fonnum ,  Astrid Molteberg ,  Silje Lien ,  Nini Kjus

IPC: B32B5/16 ,  B32B9/00 ,  C08F4/46 ,  C08F2/22 ,  C08J9/20

CPC classification number: C08F2/22 ,  C08F8/30 ,  C08F12/08 ,  C08F212/36 ,  C08F257/02 ,  C08F2800/20 ,  C08J3/128 ,  C08J9/18 ,  C08J9/20 ,  C08J2325/08 ,  C12N11/08 ,  Y10T428/2982 ,  Y10T428/2991 ,  Y10T428/2998 ,  C08F212/08

Abstract: This invention relates to monodisperse cross-linked polymer particles, comprising particles with a substantially smooth outer surface and an average diameter of less than 1 μm, wherein the particles are solid or porous, and wherein the coefficient of variation (CV) % of the particles, when measured by CPS disk centrifugation analysis, is less than 15%. These monodisperse cross-linked polymer particles may comprise magnetic material and are useful in various application. This invention also relates to monodisperse polymer particles for use as seed particles in the Ugelstad process.