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    COMPOSITIONS AND METHODS FOR DETECTING AND IDENTIFYING SALMONELLA ENTERICA STRAINS
    452.
    发明申请
    COMPOSITIONS AND METHODS FOR DETECTING AND IDENTIFYING SALMONELLA ENTERICA STRAINS 审中-公开
    用于检测和鉴定沙门氏菌菌丝菌株的组合物和方法

    公开(公告)号：US20150191778A1

    公开(公告)日：2015-07-09

    申请号：US14594397

    申请日：2015-01-12

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： CRAIG CUMMINGS ,  ELENA BOLCHAKOVA ,  MANOHAR FURTADO

    IPC: C12Q1/68 ,  C12N15/10

    CPC classification number: C12Q1/689 ,  C12N15/1089 ,  C12Q2600/156 ,  C12Q2600/158 ,  Y02A50/451

    Abstract: The present specification describes several novel SNPs of Salmonella enterica subsp. enterica. SNP profiles comprising allelic compositions at each SNP position are described which may be used to identify and differentiate different strains and serovars of Salmonella enterica subsp. enterica. The specification also describes several compositions, methods and kits useful for identifying and differentially distinguishing strains and serovars of Salmonella enterica subsp. enterica.

    Abstract translation: 本说明书描述了几种新型的肠炎沙门氏菌的SNP。 肠衣 描述了包含在每个SNP位置的等位基因组成的SNP谱，其可用于鉴定和区分不同的肠炎沙门氏菌菌株和血清型。 肠衣 本说明书还描述了用于鉴定和差异区分肠炎沙门氏菌菌株和血清型的几种组合物，方法和试剂盒。 肠衣

    Methine-Substituted Cyanine Dye Compounds
    454.
    发明申请
    Methine-Substituted Cyanine Dye Compounds 有权
    甲基取代的Cyanine染料化合物

    公开(公告)号：US20150191600A1

    公开(公告)日：2015-07-09

    申请号：US14662397

    申请日：2015-03-19

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jason DALLWIG ,  David HAGEN ,  Ching-Ying CHEUNG ,  Gerald THOMAS ,  Stephen YUE

    IPC: C09B23/10 ,  C09B23/06 ,  C12Q1/68 ,  C09B23/04

    CPC classification number: C09B23/164 ,  C07D277/64 ,  C07D417/06 ,  C09B23/0008 ,  C09B23/04 ,  C09B23/06 ,  C09B23/107 ,  C12Q1/6806 ,  C12Q1/6876 ,  C12Q2600/16 ,  G01N33/5308 ,  Y10T436/143333

    Abstract: Cyanine dye compounds having a substituted methine moiety that are nucleic acid stains, particularly for fluorescent staining of RNA, including compounds having the formula where R1 is a C1-C6 alkyl, sulfoalkyl, carboxyalkyl or C1-C6 alkoxy; each R2 is independently selected from the group consisting of H, C1-C6 alkyl, C1-C6 alkoxy, fused benzo, trifluoromethyl, amino, sulfo, carboxy and halogen, that is optionally further substituted; at least one of R3, R4, and R5 is an alkyl, aryl, heteroaryl, cyclic, or heterocyclic moiety that is optionally substituted by alkyl, amino, aminoalkyl, carboxy, nitro, or halogen; and the remaining R3, R4 or R5 are hydrogen; X is S, O, or Se; and D is a substituted or unsubstituted pyridinium, quinolinium or benzazolium moiety.

    Abstract translation: 具有作为核酸染色的取代的次甲基部分的花青染料化合物，特别是用于RNA的荧光染色，包括具有下式的化合物，其中R 1是C 1 -C 6烷基，磺基烷基，羧基烷基或C 1 -C 6烷氧基; 每个R 2独立地选自H，C 1 -C 6烷基，C 1 -C 6烷氧基，稠合的苯并，三氟甲基，氨基，磺基，羧基和卤素，其任选进一步被取代; R 3，R 4和R 5中的至少一个是任选被烷基，氨基，氨基烷基，羧基，硝基或卤素取代的烷基，芳基，杂芳基，环状或杂环部分; 剩余的R3，R4或R5是氢; X是S，O或Se; 并且D是取代或未取代的吡啶鎓，喹啉鎓或苯并咪唑鎓部分。

    POLYMER PARTICLES, NUCLEIC ACID POLYMER PARTICLES AND METHODS OF MAKING AND USING THE SAME
    457.
    发明申请
    POLYMER PARTICLES, NUCLEIC ACID POLYMER PARTICLES AND METHODS OF MAKING AND USING THE SAME 有权
    聚合物颗粒，核酸聚合物颗粒及其制备和使用方法

    公开(公告)号：US20150175734A1

    公开(公告)日：2015-06-25

    申请号：US14640009

    申请日：2015-03-05

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： David LIGHT ,  Barnett B. ROSENBLUM

    IPC: C08F251/00

    CPC classification number: C12Q1/6834 ,  C08F2/32 ,  C08F220/56 ,  C08F222/385 ,  C08F251/00 ,  C12Q1/6869 ,  C12Q2563/155 ,  C12Q2563/159

    Abstract: The disclosure relates to methods of making polymer particles, said methods including the steps of: making an aqueous gel reaction mixture; forming an emulsion having dispersed aqueous phase micelles of gel reaction mixture in a continuous phase; adding an initiator oil comprising at least one polymerization initiator to the continuous phase; and performing a polymerization reaction in the micelles. Further, the initiator oil is present in a volume % relative to a volume of the aqueous gel reaction mixture of between about 1 vol % to about 20 vol %. The disclosure also relates to methods of making nucleic acid polymer particles having the same method steps and wherein the aqueous gel reaction mixture includes a nucleic acid fragment, such as a primer.

    Abstract translation: 本公开涉及制备聚合物颗粒的方法，所述方法包括以下步骤：制备水性凝胶反应混合物; 在连续相中形成具有分散的凝胶反应混合物的水相胶束的乳液; 向连续相中加入包含至少一种聚合引发剂的引发剂油; 并在胶束中进行聚合反应。 此外，引发剂油相对于水性凝胶反应混合物的体积的体积％为约1体积％至约20体积％。 本公开还涉及制备具有相同方法步骤的核酸聚合物颗粒的方法，并且其中所述水性凝胶反应混合物包括核酸片段，例如引物。

    AMINE-CONTAINING TRANSFECTION REAGENTS AND METHODS FOR MAKING AND USING SAME
    458.
    发明申请
    AMINE-CONTAINING TRANSFECTION REAGENTS AND METHODS FOR MAKING AND USING SAME 有权
    含胺的转运试剂及其制备和使用方法

    公开(公告)号：US20150174260A1

    公开(公告)日：2015-06-25

    申请号：US14340395

    申请日：2014-07-24

    Applicant: Life Technologies Corporation

    Inventor： Zhiwei Yang ,  Parul Angrish ,  Xavier de Mollerat du Jeu ,  Kristin Wiederholt ,  Tao Wu

    IPC: A61K47/48 ,  C12N15/113 ,  A61K48/00 ,  C07C229/08

    CPC classification number: A61K47/54 ,  A61K47/543 ,  A61K48/0008 ,  C07C227/06 ,  C07C229/08 ,  C07D211/28 ,  C07D211/58 ,  C12N15/1137 ,  C12N15/88 ,  C12N2310/11 ,  C12N2310/141 ,  C07C229/26

    Abstract: There are provided for herein novel amine-containing transfection compounds and methods for making and using same. The compounds are generally obtained by reacting a primary amine with an unsaturated compound. Transfection complexes made using the amine-containing transfection compounds in combination with additional compounds to encapsulate biologically active agents such as nucleic acids are also provided for herein. Methods of using the transfection complexes for the in vivo or in vitro delivery of biologically active agents are also described. The transfection complexes of the present invention are highly potent, thereby allowing effective modulation of a biological activity at relatively low doses compared to analogous transfection compounds known in the art.

    Abstract translation: 本文提供了新颖的含胺转染化合物及其制备和使用方法。 化合物通常通过使伯胺与不饱和化合物反应而获得。 本文还提供了使用含胺转染化合物与附加化合物组合以包封生物活性剂如核酸制备的转染复合物。 还描述了使用转染复合物用于体内或体外递送生物活性剂的方法。 与本领域已知的类似转染化合物相比，本发明的转染复合物是非常有效的，从而允许相对低剂量的生物活性的有效调节。

    Methods to control dissolved gas
    460.
    发明授权
    Methods to control dissolved gas 有权
    控制溶解气体的方法

    公开(公告)号：US09045795B2

    公开(公告)日：2015-06-02

    申请号：US13802610

    申请日：2013-03-13

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： James Ball ,  David Kupec ,  David Marran ,  Jon A. Hoshizaki

    IPC: C12Q1/68

    CPC classification number: C12Q1/68 ,  C12Q1/6869 ,  C12Q1/6883 ,  G01N27/4141 ,  Y10T436/143333 ,  C12Q2527/119 ,  C12Q2565/607

    Abstract: A method of sensing nucleotide reactions includes flowing at least one nucleotide solution from a container of at least two containers of a sensor system. The sensor system includes a sensor sensitive to a byproduct of nucleotide incorporation. Each container of the at least two containers includes a different nucleotide solution. The sensor system enters an idle mode after flowing. The method further includes cycling the at least two containers through at least two cycles. Each cycle includes depressurizing the at least two containers for a first period and pressurizing the at least two containers for a second period. The method also includes pressurizing the at least two containers when the sensor system enters an active mode.

    Abstract translation: 感测核苷酸反应的方法包括从传感器系统的至少两个容器的容器流出至少一个核苷酸溶液。 传感器系统包括对核苷酸掺入的副产物敏感的传感器。 至少两个容器的每个容器都包含不同的核苷酸解决方案。 传感器系统在流动后进入空闲模式。 该方法还包括使至少两个容器循环至少两个循环。 每个循环包括使至少两个容器减压第一时间段，并且将至少两个容器加压第二周期。 该方法还包括当传感器系统进入活动模式时对至少两个容器进行加压。

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