公开(公告)号：US20230127357A1

公开(公告)日：2023-04-27

申请号：US18046967

申请日：2022-10-17

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： CHRISTOPHER BELISLE ,  JIALI LIAO ,  CALLISTA KATRINA ASUNCION ,  EDWIN LOZANO

IPC: C07K1/16

Abstract: The subject invention pertains to mixed mode chromatography ligands and chromatography matrices suitable for the purification of proteins from biological sources or biological samples. Methods of making chromatography matrices comprising the disclosed ligands and using the disclosed chromatography matrices are also provided.

公开(公告)号：US11635365B2

公开(公告)日：2023-04-25

申请号：US17686229

申请日：2022-03-03

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique

IPC: G01N15/14 ,  B01L3/00 ,  C12M1/00 ,  G01N1/20 ,  G01N1/28 ,  G01N1/40 ,  G06V20/69 ,  B01L3/02 ,  G01N35/00 ,  G01N15/00 ,  G01N15/10

Abstract: A cell capture system including an array, an inlet manifold, and an outlet manifold. The array includes a plurality of parallel pores, each pore including a chamber and a pore channel, an inlet channel fluidly connected to the chambers of the pores; an outlet channel fluidly connected to the pore channels of the pores. The inlet manifold is fluidly connected to the inlet channel, and the outlet channel is fluidly connected to the outlet channel. A cell removal tool is also disclosed, wherein the cell removal tool is configured to remove a captured cell from a pore chamber.

公开(公告)号：US20230100295A1

公开(公告)日：2023-03-30

申请号：US18075009

申请日：2022-12-05

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Ronald Lebofsky

IPC: B01L3/00 ,  B01L7/00 ,  C12Q1/686

Abstract: The invention(s) cover systems and methods for target detection in a multiplexed and rapid manner. Embodiments of the system can include: a base substrate; and an array of sample processing regions defined at a broad surface of the base substrate, wherein each of the array of sample processing regions includes: a set of microwell subarrays arranged in a gradient by volumetric capacity between an upstream end and a downstream end of each respective sample processing region, and a boundary separating each respective sample processing region from adjacent sample processing regions. The system can support methods, with example implementation by an automated platform, for returning preliminary results from a subset of microwells of the samples processing regions, as well as results pertaining to specific and non-specific amplification, for multiple targets of a sample.

公开(公告)号：US20230086845A1

公开(公告)日：2023-03-23

申请号：US17992304

申请日：2022-11-22

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Jonathan William Larson ,  Qun Zhong ,  Darren Roy Link

IPC: C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/686

Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.

公开(公告)号：US11603556B2

公开(公告)日：2023-03-14

申请号：US17087952

申请日：2020-11-03

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald Lebofsky

IPC: C12Q1/68 ,  C12Q1/6834 ,  C12Q1/6855

Abstract: Methods and compositions for attaching cell-specific barcodes without formation of partitions is provided.

公开(公告)号：US20230015223A1

公开(公告)日：2023-01-19

申请号：US17951719

申请日：2022-09-23

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Austin Payne ,  Vishal Sharma ,  Kyle Gleason ,  Priyadarshini Gogoi ,  Karthik Ganesan ,  Brian Boniface ,  Will Chow

IPC: G01N15/14 ,  G01N1/20 ,  G01N1/40 ,  G01N1/28 ,  C12M1/00 ,  B01L3/00 ,  G01N35/10 ,  G01N35/00 ,  G06V10/141 ,  G06V10/145 ,  G06V10/147 ,  G06V20/69

Abstract: A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.

公开(公告)号：US11504719B2

公开(公告)日：2022-11-22

申请号：US16816817

申请日：2020-03-12

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Austin Payne

IPC: B01L99/00 ,  B01L3/00 ,  G01N1/00

Abstract: A system and method for receiving and delivering a fluid, the system comprising: a body configured to interface with an opening of a reservoir and defining: a protrusion defining a set position of the body relative to the reservoir; a wall extending from the protrusion; a receiving surface coupled to the wall and sloping from an apex to a nadir along a first direction, the receiving surface comprising a vent; and an outlet positioned closer to the nadir than the apex of the receiving surface and displaced from the vent, the outlet comprising an extension from the body, the extension configured to contact an interior wall of the reservoir, wherein the body comprises: a bubble-mitigating operation mode in which the receiving surface receives and transmits the fluid along the receiving surface, and a fluid-transmitting operation mode in which the body directs the fluid along the interior wall of the reservoir.

公开(公告)号：US11499183B2

公开(公告)日：2022-11-15

申请号：US16022500

申请日：2018-06-28

Applicant: Bio-Rad Laboratories, Inc.

Inventor： David P. Stumbo ,  George Carman ,  Steve Hobbs ,  Anthony J. Makarewicz, Jr. ,  Dmitri Simonian ,  David Glade ,  Joshua Oen ,  Denis Pristinski ,  John Dzenitis

IPC: G01N15/14 ,  B01F3/08 ,  C12Q1/6848 ,  C12Q1/6844 ,  C12Q1/686 ,  B01L3/00 ,  C12Q1/6851 ,  B01F23/41

Abstract: Systems and methods for detection of a signal from droplets of an emulsion. An exemplary system may comprise a fluid transporter having a tube with an open end for aspirating droplets, a singulator to arrange the droplets in single file and to space the single-file droplets from one another, and a detection channel in optical communication with a detector configured to detect a signal from droplets. In some embodiments, the singulator may have a channel junction at which a stream of droplets in single file is combined with a stream of spacing fluid, and a tapered spacing channel extending downstream from the channel junction toward the detection channel. In some embodiments, the fluid transporter may suck droplet-containing fluid and spacing fluid through the detection channel from respective sources. In some embodiments, droplets may be subjected to a disaggregation routine before they are passed through the detection channel.

公开(公告)号：US11479816B2

公开(公告)日：2022-10-25

申请号：US16545611

申请日：2019-08-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald Lebofsky ,  Andrew Kohlway ,  Jennifer Chew ,  Zachary Burkett ,  Man Cheng

IPC: C12Q1/6869 ,  G16B40/10 ,  G16B30/10

Abstract: Comparison of common sequencing reads from sequencing based on partition-based barcoding can be used to improve sequencing results. Increased loading of barcodes per partition can also improve sequencing results.

公开(公告)号：US11435285B2

公开(公告)日：2022-09-06

申请号：US17199312

申请日：2021-03-11

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Evan Thrush ,  Steven Swihart ,  William Strong ,  Trey Marlowe ,  Li Lu

IPC: G01N21/31 ,  G01N21/3577 ,  G01N21/3563

Abstract: Systems for protein quantitation using a Fabry-Perot interferometer. In one arrangement, a quantitation device includes an infrared source, a sample holder, and a Fabry-Perot interferometer positioned to receive infrared radiation from the source passing through a sample on the sample holder. A band pass optical filter sets the working range of the interferometer, and radiation exiting the interferometer falls on a detector that produces a signal indicating the intensity of the received radiation. A controller causes the interferometer to be tuned to a number of different resonance wavelengths and receives the intensity signals, for determination of an absorbance spectrum.