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    Nucleotide sequences coding for the export of branched-chain amino acids, process for the isolation thereof and use thereof
    44.
    发明授权
    Nucleotide sequences coding for the export of branched-chain amino acids, process for the isolation thereof and use thereof 有权
    编码支链氨基酸输出的核苷酸序列，其分离方法及其用途

    公开(公告)号：US06841360B2

    公开(公告)日：2005-01-11

    申请号：US10608504

    申请日：2003-06-30

    Applicant: Nicole Kennerknecht ,  Hermann Sahm ,  Lothar Eggeling ,  Walter Pfefferie

    Inventor： Nicole Kennerknecht ,  Hermann Sahm ,  Lothar Eggeling ,  Walter Pfefferie

    IPC: C12N15/09 ,  C07K14/34 ,  C12N1/21 ,  C12N15/31 ,  C12N15/54 ,  C12P13/06 ,  C12P13/08 ,  C12R1/15 ,  C12P21/06

    CPC classification number: C07K14/34 ,  C12P13/06 ,  C12P13/08

    Abstract: This invention relates to isolated polynucleotides containing at least one of the polynucleotide sequences selected from the group a) polynucleotide which is at least 70% identical to a polynucleotide which codes for a polypeptide containing at least one amino acid sequence of SEQ ID no. 3 or 5, b) polynucleotide which codes for a polypeptide which contains an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID no. 3 or 5, c) polynucleotide which is complementary to the polynucleotides of a), b) or c), and d) polynucleotide containing at least 15 successive bases of the polynucleotide sequences of a), b) or c). wherein the polypeptides exhibit the biological activity of the enzymes for which the brnE or bernF (sic)gene codes and a process for the fermentative production of branched-chain L-amino acids with amplification of the stated genes.

    Abstract translation: 本发明涉及分离的多核苷酸，其含有至少一种多核苷酸序列，所述多核苷酸序列选自a组多核苷酸，所述多核苷酸序列与编码含有至少一个氨基酸序列SEQ ID NO：1的多核苷酸至少70％相同。 3或5，b）编码多肽的多核苷酸，其含有与SEQ ID No.3的氨基酸序列至少70％相同的氨基酸序列。 3或5，c）与a），b）或c）的多核苷酸互补的多核苷酸，和d）包含a），b）或c）的多核苷酸序列的至少15个连续碱基的多核苷酸。 brnE或bernF（sic）基因编码的酶的生物活性以及用所述基因扩增产生支链L-氨基酸的方法。

    Process for the fermentative preparation of L-amino acids using coryneform bacteria
    45.
    发明授权
    Process for the fermentative preparation of L-amino acids using coryneform bacteria 失效
    使用棒状细菌发酵制备L-氨基酸的方法

    公开(公告)号：US06596516B2

    公开(公告)日：2003-07-22

    申请号：US09731826

    申请日：2000-12-08

    Applicant: Petra Ziegler ,  Lothar Eggeling ,  Hermann Sahm ,  Georg Thierbach ,  Walter Pfefferle

    Inventor： Petra Ziegler ,  Lothar Eggeling ,  Hermann Sahm ,  Georg Thierbach ,  Walter Pfefferle

    IPC: C12P1304

    CPC classification number: C12N9/1014 ,  C12P13/04 ,  C12P13/08

    Abstract: A process for the preparation of L-amino acids, in which the following steps are carried out, a) fermenting the desired L-amino acid-producing bacteria in which at least the glyA gene is attenuated, in particular by removal of the natural promoter, and optionally b) concentrating the desired product in the medium or in the cells of the bacteria and c) isolating the L-amino acid, and optionally bacteria in which further genes of the biosynthesis pathway of the desired L-amino acid are additionally amplified are employed, or bacteria in which the metabolic pathways which reduce the formation of the desired L-amino acid are at least partly eliminated are employed, and nucleotide sequences of the lacI-tac-5′glyA or lacI-tac-glyA unit.

    Abstract translation: 一种制备L-氨基酸的方法，其中进行以下步骤：a）将至少所述glyA基因减毒的所需L-氨基酸产生细菌发酵，特别是通过除去天然启动子 并且任选地b）将所需产物浓缩在细菌的培养基中或细胞中，并且c）分离L-氨基酸，以及任选的细菌，其中另外扩增所需L-氨基酸的生物合成途径的其它基因的细菌 或其中代谢途径减少所需L-氨基酸的形成至少部分消除的细菌，以及lacI-tac-5'glyA或lacI-tac-glyA单元的核苷酸序列。

    Process for the microbial production of alcoholoxidase
    49.
    发明授权
    Process for the microbial production of alcoholoxidase 失效
    藻酸酶微生物生产工艺

    公开(公告)号：US4250261A

    公开(公告)日：1981-02-10

    申请号：US55533

    申请日：1979-07-09

    Applicant: Lothar Eggeling ,  Manfred Paschke ,  Hermann Sahm

    Inventor： Lothar Eggeling ,  Manfred Paschke ,  Hermann Sahm

    IPC: C12N9/04 ,  C12R1/78

    CPC classification number: C12N9/0006 ,  Y10S435/93

    Abstract: A method of producing alcohol oxidase wherein Hansenula polymorpha yeast is cultivated in a nutrient broth containing inorganic nutrient salts of which either the phosphate, nitrate or potassium is present in a cell-growth-limiting concentration at a temperature of 25.degree. to 45.degree. C. and a pH of 4.0 to 6.0 with oxygen being supplied as air for a mixture of air and oxygen. According to the invention, an organic substrate consisting of glycerin, sorbite or xylose, noncatabolite-repressive to alcohol-oxidase is added to the broth in a concentration of 0.1 to 1.0% in a nutrient solution at a rate of 0.03 to 0.15 .sup.-1.

    Abstract translation: 一种生产醇氧化酶的方法，其中将多形汉逊酵母（Hansenula polymorpha yeast）培养在含有无机营养盐的营养肉汤中，其中磷酸盐，硝酸盐或钾以25至45℃的温度存在于细胞生长限制浓度中。 pH为4.0至6.0，氧气作为空气和氧气的混合物供应。 根据本发明，在营养液中以0.03-0.15-1的比例，在肉汤中以0.1至1.0％的浓度加入由甘油，山梨糖醇或木糖组成的有机底物，对醇醇氧化酶具有抑制作用。

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