利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

51 条记录 (耗时 0.024 秒)

    Chimeric peptide immunogens
    42.
    发明申请
    Chimeric peptide immunogens 审中-公开
    嵌合肽免疫原

    公开(公告)号：US20070184064A1

    公开(公告)日：2007-08-09

    申请号：US10886038

    申请日：2004-07-06

    申请人： Stephen Grimes ,  Dov Michaeli ,  Vernon Stevens

    发明人： Stephen Grimes ,  Dov Michaeli ,  Vernon Stevens

    IPC分类号： A61K39/12

    CPC分类号： A61K39/0006 ,  A61K39/00 ,  A61K2039/6031 ,  A61K2039/6037 ,  A61K2039/6068 ,  C07K7/23 ,  C07K2319/00 ,  Y02A50/412

    摘要： Chimeric peptide epitopes can serve as effective immunogens against hormones and other small peptides or proteins. Thus, immunogenic peptides are selected from promiscuous Th epitopes and synthesized together with self antigenic peptide sequences fused with or without end to end spacer peptide interconnections. A peptide sequence which may be of the gonadotropin releasing hormone is linked with an immunogenic peptide sequence selected from a promiscuous Th-epitope of measles virus protein F, tetanus toxoid, or malaria protein CSP. Compositions of the chimeric immunogen are found effective in eliciting high and specific anti-GnRH antibody titers.

    摘要翻译： 嵌合肽表位可以作为针对激素和其他小肽或蛋白质的有效免疫原。 因此，免疫原性肽选自混杂的Th表位，并与与或不与端到端间隔肽互连融合的自身抗原肽序列一起合成。 可以是促性腺激素释放激素的肽序列与选自麻疹病毒蛋白F，破伤风类毒素或疟疾蛋白CSP的混杂Th-表位的免疫原性肽序列连接。 发现嵌合免疫原的组合物在引发高和特异性抗GnRH抗体滴度方面是有效的。

    Gastrin hormone immunoassays
    43.
    发明申请
    Gastrin hormone immunoassays 有权
    胃泌素激素免疫测定

    公开(公告)号：US20050069966A1

    公开(公告)日：2005-03-31

    申请号：US10813336

    申请日：2004-03-29

    申请人： Stephen Grimes ,  John Little ,  Lorraine McLoughlin

    发明人： Stephen Grimes ,  John Little ,  Lorraine McLoughlin

    IPC分类号： C07K16/26 ,  G01N33/53 ,  G01N33/537 ,  G01N33/543 ,  G01N33/68 ,  G01N33/74

    CPC分类号： G01N33/6893 ,  G01N33/74 ,  G01N2333/595

    摘要： The invention provides assay methods for the detection and quantitation of gastrin hormones, including total and free gastrin hormone in a sample. ELISA-type heterogeneous phase assays suitable for use with biological fluid samples such as blood, plasma or other bodily fluids of a mammal, particularly a human subject are provided. The method provides a precise assay for the amounts of free and total G17 and G34 in biological fluid samples, as well as the amounts of free and total Gly-extended G17, and Gly-extended G34. Also provided are methods of determining suitable treatment for patient suffering from a gastrin hormone-mediated disease or condition employing gastrin hormone immunoassays.

    摘要翻译： 本发明提供了用于检测和定量胃泌素激素的测定方法，包括样品中的总的和游离的胃泌素激素。 提供了适用于哺乳动物，特别是人类受试者的生物流体样品如血液，血浆或其它体液的ELISA型异相测定。 该方法提供了生物流体样品中游离和总G17和G34的量的精确测定，以及游离和总Gly扩展的G17和Gly延伸的G34的量。 还提供了确定患有胃泌素激素介导的疾病或使用胃泌素激素免疫测定的病症的患者的适合治疗的方法。

    Monoclonal antibodies to gastrin hormone
    48.
    发明申请
    Monoclonal antibodies to gastrin hormone 审中-公开
    胃泌素激素单克隆抗体

    公开(公告)号：US20070066809A1

    公开(公告)日：2007-03-22

    申请号：US11499261

    申请日：2006-08-03

    申请人： Stephen Grimes ,  Ronald Makishima

    发明人： Stephen Grimes ,  Ronald Makishima

    IPC分类号： C07K16/00

    CPC分类号： C07K16/26 ,  A61K2039/505 ,  C07K2317/34 ,  C07K2317/73 ,  G01N33/577 ,  G01N33/74 ,  G01N2333/595

    摘要： The present invention provides monoclonal antibodies (MAbs) selective for the N-termini and C-termini of the gastrin hormone forms, gastrin-17 (G17), glycine-extended gastrin-17 (G17-Gly), gastrin-34 (G34) and glycine-extended gastrin-34 (G34-Gly); and the hybridomas that produce these MAbs. Also provided are panels of MAbs useful for the detection and quantitation of gastrin-17 (G17), glycine-extended gastrin-17 (G17-Gly), gastrin-34 (G34) and glycine-extended gastrin-34 (G34-Gly). These assays are useful for monitoring a gastrin-mediated disease or condition, or for monitoring the progress of a course of therapy. The invention further provides solid phase assays including immunohistochemical (IHC) and immunofluorescence (IF) assays suitable for detection and visualization of gastrin species in solid samples, such as biopsy samples or tissue slices. Pharmaceutical compositions of the MAbs of the invention are also provided, along with methods of diagnosis, prevention and treatment of gastrin-mediated diseases or conditions. Methods of evaluating a gastrin hormone-blocking treatment are described. The course of a gastrin-mediated disease or condition may be monitored in a patient by means of assay methods provided.

    摘要翻译： 本发明提供对胃泌素激素形式的N-末端和C-末端选择性的单克隆抗体（MAb），胃泌素-17（G17），甘氨酸延长胃泌素-17（G17-Gly），胃泌素-34（G34） 和甘氨酸延长胃泌素-34（G34-Gly）; 和产生这些MAb的杂交瘤。 还提供了可用于检测和定量胃泌素-17（G17），甘氨酸延长胃泌素-17（G17-Gly），胃泌素-34（G34）和甘氨酸延长胃泌素-34（G34-Gly） 。 这些测定法可用于监测胃泌素介导的疾病或病症，或用于监测治疗过程的进展。 本发明进一步提供固相测定法，包括免疫组织化学（IHC）和免疫荧光（IF）测定法，其适于检测和可视化固体样品例如活检样本或组织切片中的胃泌素种类。 还提供本发明的MAb的药物组合物以及胃泌素介导的疾病或病症的诊断，预防和治疗的方法。 描述了评估胃泌素激素阻断治疗的方法。 胃泌素介导的疾病或病症的过程可以通过提供的测定方法在患者体内监测。