公开(公告)号：US09951386B2

公开(公告)日：2018-04-24

申请号：US15717871

申请日：2017-09-27

Applicant: 10X GENOMICS, INC.

Inventor： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Serge Saxonov

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/683 ,  C12Q2525/191 ,  C12Q2537/143 ,  C12Q2563/179 ,  C12Q2535/122 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/629

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US20180094313A1

公开(公告)日：2018-04-05

申请号：US15831847

申请日：2017-12-05

Applicant: 10X Genomics, Inc.

Inventor： Benjamin Hindson ,  Christopher Hindson ,  Micheal Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Serge Saxonov ,  Paul Hardenbol ,  Rajiv Bharadwaj ,  Xinying Zheng ,  Phillip Belgrader

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/683 ,  C12Q2525/191 ,  C12Q2535/122 ,  C12Q2537/143 ,  C12Q2537/149 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/629

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US20170362587A1

公开(公告)日：2017-12-21

申请号：US15693374

申请日：2017-08-31

Applicant: 10X GENOMICS, INC.

Inventor： Benjamin Hindson ,  Serge Saxonov ,  Kevin Ness ,  Paul Hardenbol ,  Christopher Hindson ,  Donald Masquelier ,  Mirna Jarosz ,  Michael Schnall-Levin

IPC: C12N15/10 ,  C12Q1/68 ,  C12N15/11 ,  G01N33/543

CPC classification number: C12N15/1065 ,  C12N15/11 ,  C12Q1/6806 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q2600/112 ,  G01N33/5436

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US20170260584A1

公开(公告)日：2017-09-14

申请号：US15430298

申请日：2017-02-10

Applicant: 10X Genomics, Inc. ,  Fred Hutchinson Cancer Research Center

Inventor： Xinying Zheng ,  Jason H. Bielas ,  Mark T. Gregory ,  Benjamin Hindson ,  Tarjei Sigurd Mikkelsen ,  Ryan Wilson ,  Paul Ryvkin

IPC: C12Q1/68 ,  C12N15/10

CPC classification number: C12Q1/6881 ,  C12N15/1065 ,  C12N15/1093 ,  C12Q2600/156 ,  G01N2015/1006

Abstract: The disclosure provides methods and systems for producing single cell RNA sequencing data. Single nucleotide polymorphisms (SNPs) identified in such data can be used to distinguish subpopulations of cells within a mixed population.

公开(公告)号：US20170247757A1

公开(公告)日：2017-08-31

申请号：US15588519

申请日：2017-05-05

Applicant: 10X Genomics, Inc.

Inventor： Benjamin Hindson ,  Serge Saxonov ,  Kevin Ness ,  Paul Hardenbol ,  Michael Schnall-Levin ,  Mirna Jarosz

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q2525/161 ,  C12Q2535/122 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/514

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US20160281161A1

公开(公告)日：2016-09-29

申请号：US15174922

申请日：2016-06-06

Applicant: 10X Genomics, Inc.

Inventor： Mirna Jarosz ,  Michael Schnall-Levin ,  Serge Saxonov ,  Benjamin Hindson ,  Xinying Zheng

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6874 ,  C12Q2535/122 ,  C12Q2537/159 ,  C12Q2565/514 ,  C12Q2563/179

Abstract: The present invention is directed to methods, compositions and systems for capturing and analyzing sequence information contained in targeted regions of a genome. Such targeted regions may include exomes, partial exomes, introns, combinations of exonic and intronic regions, genes, panels of genes, and any other subsets of a whole genome that may be of interest.

公开(公告)号：US20160122817A1

公开(公告)日：2016-05-05

申请号：US14927297

申请日：2015-10-29

Applicant: 10X Genomics, Inc.

Inventor： Mirna Jarosz ,  Michael Schnall-Levin ,  Serge Saxonov ,  Benjamin Hindson ,  Xinying Zheng

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6874 ,  C12Q2535/122 ,  C12Q2537/159 ,  C12Q2565/514 ,  C12Q2563/179

Abstract: The present invention is directed to methods, compositions and systems for capturing and analyzing sequence information contained in targeted regions of a genome. Such targeted regions may include exomes, partial exomes, introns, combinations of exonic and intronic regions, genes, panels of genes, and any other subsets of a whole genome that may be of interest.

公开(公告)号：US20160121325A1

公开(公告)日：2016-05-05

申请号：US14934044

申请日：2015-11-05

Applicant: 10X Genomics, Inc.

Inventor： Donald A. Masquelier ,  Benjamin Hindson ,  Kevin Ness ,  Rajiv Bharadwaj

IPC: B01L3/00 ,  B01L9/00 ,  C12Q1/68 ,  F16K99/00

CPC classification number: B01L3/50273 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2300/043 ,  B01L2300/0609 ,  B01L2300/0636 ,  B01L2300/0864 ,  B01L2300/10 ,  B01L2300/14 ,  B01L2300/18 ,  B01L2400/0487 ,  B01L2400/0605 ,  C12Q1/6874 ,  F16K99/0057 ,  F16K2099/0084

Abstract: An integrated system for processing and preparing samples for analysis may include a microfluidic device including a plurality of parallel channel networks for partitioning the samples including various fluids, and connected to a plurality of inlet and outlet reservoirs, at least a portion of the fluids comprising reagents, a holder including a closeable lid hingedly coupled thereto, in which in a closed configuration, the lid secures the microfluidic device in the holder, and in an open configuration, the lid is a stand orienting the microfluidic device at a desired angle to facilitate recovery of partitions or droplets from the partitioned samples generated within the microfluidic device, and an instrument configured to receive the holder and apply a pressure differential between the plurality of inlet and outlet reservoirs to drive fluid movement within the channel networks.

Abstract translation: 用于处理和制备用于分析的样品的集成系统可以包括微流体装置，其包括用于分隔包括各种流体的样品并连接到多个入口和出口储存器的多个平行通道网络，至少一部分流体包含试剂 ，包括铰接地联接到其上的可关闭盖的保持器，其中在闭合构造中，盖将微流体装置固定在保持器中，并且在打开构造中，盖是以所需角度定向微流体装置的支架，以便于恢复 来自在微流体装置内产生的分隔样品的分隔物或液滴;以及被配置为接收保持器并在多个入口和出口储存器之间施加压力差以驱动通道网络内的流体运动的仪器。

公开(公告)号：US20150292988A1

公开(公告)日：2015-10-15

申请号：US14682952

申请日：2015-04-09

Applicant: 10X Genomics, Inc.

Inventor： Rajiv Bharadwaj ,  Kevin Ness ,  Debkishore Mitra ,  Donald Masquelier ,  Anthony Makarewicz ,  Christopher Hindson ,  Benjamin Hindson ,  Serge Saxonov

IPC: G01N1/28 ,  B01L3/00

CPC classification number: B01L3/502761 ,  B01L3/0241 ,  B01L3/502784 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2400/049 ,  B01L2400/086 ,  G01N35/085 ,  G01N2035/00237 ,  G01N2035/1034

Abstract: The disclosure provides devices, systems and methods for the generation of encapsulated reagents and the partitioning of encapsulated reagents for use in subsequent analyses and/or processing, such as in the field of biological analyses and characterization.

Abstract translation: 本公开提供了用于产生封装的试剂的装置，系统和方法以及用于后续分析和/或加工的包封试剂的分配，例如在生物分析和表征领域。

公开(公告)号：US20150218633A1

公开(公告)日：2015-08-06

申请号：US14680808

申请日：2015-04-07

Applicant: 10X Genomics, Inc.

Inventor： Benjamin Hindson ,  Serge Saxonov ,  Kevin Ness ,  Paul Hardenbol

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/683 ,  C12Q2525/191 ,  C12Q2537/143 ,  C12Q2563/179

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

Abstract translation: 本公开提供了用于多核苷酸处理的组合物，方法，系统和装置。 这种多核苷酸处理可用于多种应用，包括多核苷酸测序。