公开(公告)号：US20220134338A1

公开(公告)日：2022-05-05

申请号：US17516084

申请日：2021-11-01

Applicant: Cepheid

Inventor： Ronald Chang ,  Steven M. Montgomery ,  Gregory E. Mote ,  Brian M. Bliven

IPC: B01L3/00

Abstract: A biological sample processing apparatus having an enclosure and a plurality of sample processing modules held within an enclosure with a tiltable graphical user interface screen. In one aspect, the individual modules that are readily removable for repair, replacement or upgrade. Each module is configured to be independently operable and readily inserted into the enclosure for connection with a processing unit of the enclosure. Each module can include quick-release mechanisms so that the module can be readily removed and replaced manually or with minimal tools through the front of the enclosure without requiring substantial or total disassembly of the module or entire enclosure. In another aspect, the user interface screen can display identifying information, such as a barcode, that can be scanned by a user's portable device so as to monitor the progress of an assay remotely.

公开(公告)号：US09943848B2

公开(公告)日：2018-04-17

申请号：US14737853

申请日：2015-06-12

Applicant: CEPHEID

Inventor： Michael T. Taylor ,  Phillip Belgrader ,  Farzad Pourahmadi ,  William A. McMillan ,  Ronald Chang ,  Stanley H. Sakai ,  Jesus Ching

IPC: B01L3/00 ,  B01L1/00 ,  C12M1/00 ,  C12N1/06 ,  C12N13/00

CPC classification number: B01L3/508 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502761 ,  B01L2200/025 ,  B01L2300/0848 ,  B01L2300/12 ,  C12M47/06 ,  C12N1/066 ,  C12N13/00

Abstract: An apparatus for disrupting cells or viruses comprises a container having a chamber for holding the cells or viruses. The container includes at least one flexible wall defining the chamber. The apparatus also includes a transducer for impacting an external surface of the flexible wall to generate pressure waves in the chamber. The apparatus also includes a pressure source for increasing the pressure in the chamber. The pressurization of the chamber ensures effective coupling between the transducer and the flexible wall. The apparatus may also include beads in the chamber for rupturing the cells or viruses.

公开(公告)号：US09873909B2

公开(公告)日：2018-01-23

申请号：US14553622

申请日：2014-11-25

Applicant: Cepheid

Inventor： Joseph H. Smith ,  David H. Persing ,  Alan Wortman ,  Ronald Chang ,  David Swenson

IPC: C12P19/34 ,  C12Q1/68 ,  B01L3/00 ,  B01L7/00

CPC classification number: C12Q1/686 ,  B01L3/502738 ,  B01L7/52 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0644 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/689 ,  C12Q2565/629 ,  C12Q2547/101 ,  C12Q2537/149 ,  C12Q2537/143 ,  C12Q2565/101 ,  C12Q2527/107

Abstract: The invention provides methods and apparatus for carrying out multiple amplification reactions in a single reaction chamber by successive cycles of loading reaction mixture, amplifying, and removing spent reaction mixture in a fluidly closed reaction system. In particular, the present invention allows amplification of a plurality of target polynucleotides from a single sample by carrying out under closed-loop control successive amplifications of different target polynucleotides from different portions of the sample.

公开(公告)号：US09212980B2

公开(公告)日：2015-12-15

申请号：US14169402

申请日：2014-01-31

Applicant: Cepheid

Inventor： Douglas Dority ,  Ronald Chang

IPC: B01L3/00 ,  G01N1/40 ,  G01N1/28 ,  G01N1/18

CPC classification number: B01L3/502715 ,  B01L3/502 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2200/06 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/06 ,  B01L2300/0645 ,  B01L2300/0681 ,  B01L2300/0848 ,  B01L2300/0861 ,  B01L2300/0877 ,  B01L2300/123 ,  B01L2300/14 ,  B01L2400/0421 ,  B01L2400/0478 ,  B01L2400/0487 ,  B01L2400/0644 ,  G01N1/18 ,  G01N1/28 ,  G01N1/405 ,  Y10T436/255 ,  Y10T436/2575

Abstract: A fluid control and processing system for controlling fluid flow among a plurality of chambers comprises a body including a fluid processing region continuously coupled fluidicly with a fluid displacement region. The fluid displacement region is depressurizable to draw fluid into the fluid displacement region and pressurizable to expel fluid from the fluid displacement region. The body includes at least one external port. The fluid processing region is fluidicly coupled with the at least one external port. The fluid displacement region is fluidicly coupled with at least one external port of the body. The body is adjustable with respect to the plurality of chambers to place the at least one external port selectively in fluidic communication with the plurality of chambers. One or more of the chambers may be a processing chamber which includes two ports configured to selectively engage the at least one external port of the body, and a fluid processing material such as an enrichment material or a depletion material. In some embodiments, one or more chambers may include a separation channel, and an electric field may be applied across the separation channel.

公开(公告)号：US20140030817A1

公开(公告)日：2014-01-30

申请号：US13849640

申请日：2013-03-25

Applicant: Cepheid

Inventor： Ronald Chang ,  Douglas B. Dority ,  Steven Montgomery

IPC: B01L3/00

CPC classification number: B01L3/527 ,  B01J19/0046 ,  B01J2219/00468 ,  B01J2219/005 ,  B01J2219/00657 ,  B01L3/0289 ,  B01L2200/0647 ,  B01L2200/16 ,  G01N2035/00574 ,  Y10T436/25

Abstract: Embodiments of the invention provide an efficient and effective technique for storing and dispensing reagent beads. In one embodiment, an apparatus is provided for dispensing reagent beads contained in a bead storage device which includes a bead carrier having a plurality of wells; a plurality of reagent beads disposed in the wells; and a cover tape releasably attached to the bead carrier to cover the wells and retain the reagent beads in the wells. The apparatus comprises a channel in which to place the bead storage device with the bead carrier facing a support wall of the channel and the cover tape facing a stripping wall of the channel. The stripping wall includes a stripping gap disposed between a stripping edge and an opposite edge, and a dispense opening provided adjacent the opposite edge on a side of the stripping wall opposite from the stripping edge. The cover tape is insertable through the stripping gap to be pulled against the stripping edge to peel the cover tape from the bead carrier to move the wells of the bead carrier inside the channel toward the dispense opening and expose the wells individually to dispense the reagent beads.

Abstract translation: 本发明的实施方案提供了用于存储和分配试剂珠的有效和有效的技术。 在一个实施例中，提供了一种用于分配包含在珠子存储装置中的试剂珠粒的装置，其包括具有多个孔的珠粒载体; 设置在所述孔中的多个试剂珠; 以及可剥离地附接到胎圈载体以覆盖孔并将试剂珠保持在孔中的覆盖带。 该装置包括一个通道，在该通道中，珠子存放装置使胎圈承载件面向通道的支撑壁，盖带面向通道的剥离壁。 剥离壁包括设置在剥离边缘和相对边缘之间的剥离间隙，以及在剥离壁的与剥离边缘相对的一侧上邻近相对边缘设置的分配开口。 覆盖带可以通过剥离间隙被插入以被拉靠在剥离边缘上，以从胎圈载体上剥离覆盖带，以将通道内的胎圈载体的孔移动到分配开口，并单独暴露孔以分配试剂珠 。

公开(公告)号：US20130220931A1

公开(公告)日：2013-08-29

申请号：US13862342

申请日：2013-04-12

Applicant: CEPHEID

Inventor： Kurt E. Petersen ,  William A. McMillan ,  Lee A. Christel ,  Ronald Chang ,  Farzad Pourahmadi ,  Jesus Ching ,  Gregory T.A. Kovacs ,  M. Allen Northrup

IPC: B01D15/00

CPC classification number: C12N15/1017 ,  B01D15/00 ,  B01L7/52 ,  B01L2400/0622 ,  B01L2400/0644 ,  C12M47/06 ,  G01N1/34 ,  Y10T436/255

Abstract: An analyte is separated from a fluid sample by introducing the sample into a cartridge having a sample port and a first flow path extending from the sample port. The first flow path includes an extraction chamber containing a solid support for capturing the analyte from the sample. The cartridge has a second flow path for eluting the captured analyte from the extraction chamber, the second flow diverging from the first flow path after passing through the extraction chamber. The sample is forced to flow through the extraction chamber and into a waste chamber, thereby capturing the analyte with the solid support as the sample flows through the extraction chamber. The captured analyte is then eluted from the extraction chamber by forcing an elution fluid to flow through the extraction chamber and along the second flow path.