公开(公告)号：US5679533A

公开(公告)日：1997-10-21

申请号：US479390

申请日：1995-06-07

Applicant: Przemyslaw Szafranski ,  Charlene M. Mello ,  Takeshi Sano ,  Kenneth A. Marx ,  Charles R. Cantor ,  David L. Kaplan ,  Cassandra L. Smith

Inventor： Przemyslaw Szafranski ,  Charlene M. Mello ,  Takeshi Sano ,  Kenneth A. Marx ,  Charles R. Cantor ,  David L. Kaplan ,  Cassandra L. Smith

IPC: C07K14/36 ,  C07K14/465 ,  C12N1/21 ,  C12N15/12 ,  C12N15/70 ,  G01N33/53

CPC classification number: C12N15/70 ,  C07K14/36 ,  C07K14/465

Abstract: The present invention relates to genetic containment systems which express a biotin-binding component that can be used for selectively destroying recombinant cells such as genetically engineered microorganisms. These systems may comprise a streptavidin or an avidin gene whose expression is controlled by a regulatable promoter. The regulatory agent such as a transcriptional effector is expressed from another gene which may also be expressed and its expression controlled by the containment system. Expression of the agent can be designed to respond to physiological changes in the environment. The invention also relates to containment systems and methods for the selective detection or tracking of recombinant cells and to eukaryotic and prokaryotic cells which contain these genetic containment systems.

公开(公告)号：US5665539A

公开(公告)日：1997-09-09

申请号：US131301

申请日：1993-10-04

Applicant: Takeshi Sano ,  Charles R. Cantor ,  Cassandra L. Smith

Inventor： Takeshi Sano ,  Charles R. Cantor ,  Cassandra L. Smith

IPC: A61K38/00 ,  A61K47/48 ,  C07K14/31 ,  C07K14/36 ,  C12Q1/68 ,  C12Q1/66

CPC classification number: C07K14/31 ,  B82Y5/00 ,  C07K14/36 ,  A61K38/00

Abstract: A novel system and method for sensitive antigen detection. The system utilizes immuno-polymerase chain reaction in which a specific biotinylated nucleic acid molecule is used as the marker. The biotinylated marker is attached to antigen-antibody complex through a streptavidin-protein A chimeric protein that possesses tight and specific binding affinity both for biotin and immunoglobulin G. A segment of the attached biotinylated marker is amplified by polymerase chain reactions with appropriate primers and the polymerase chain reaction products are detected by agarose gel electrophoresis. The method can detect any antigen and has a greater sensitivity than any existing antigen detection system.

Abstract translation: 一种用于敏感抗原检测的新型系统和方法。 该系统利用免疫聚合酶链反应，其中使用特异性生物素化的核酸分子作为标记。 生物素标记通过链霉抗生物素蛋白 - 蛋白A嵌合蛋白连接到抗原 - 抗体复合物上，该嵌合蛋白对于生物素和免疫球蛋白G都具有紧密和特异性的结合亲和力。通过与适当引物的聚合酶链反应扩增连接的生物素标记的片段，并且 通过琼脂糖凝胶电泳检测聚合酶链反应产物。 该方法可以检测任何抗原并且比任何现有的抗原检测系统具有更高的灵敏度。

公开(公告)号：US5635602A

公开(公告)日：1997-06-03

申请号：US107186

申请日：1993-08-13

Applicant: Charles R. Cantor ,  Roy S. Chuck ,  Doris B. Tse

Inventor： Charles R. Cantor ,  Roy S. Chuck ,  Doris B. Tse

IPC: C07K16/28 ,  C07K16/46 ,  C07K17/00 ,  C07K19/00

CPC classification number: C07K16/2812 ,  C07K16/2809 ,  C07K16/468

Abstract: The invention relates to bis-protein-DNA conjugates. A protein having an antigen specific binding activity is covalently linked to each end of a derivatized DNA molecule. The bis-protein-DNA conjugates can be used for immunoassays and measuring distances between proteins at up to 3.4 .ANG. resolution. The invention also relates to methods of synthesizing these bis-protein-DNA conjugates. Synthesis of the conjugates entails derivatizing the 5' or 3' end of a DNA oligonucleotide and covalently linking that DNA to a protein. The DNA can be indirectly conjugated to an antibody or Fab' fragment, using a avidin/streptavidin-biotin linkage. The conjugates of the invention can be used in immunoassays and PCR assays.

Abstract translation: 本发明涉及双蛋白-DNA缀合物。 具有抗原特异性结合活性的蛋白质与衍生的DNA分子的每个末端共价连接。 双蛋白-DNA缀合物可用于免疫测定，并以高达3.4 ANGSTROM分辨率测量蛋白质之间的距离。 本发明还涉及合成这些双蛋白-DNA缀合物的方法。 共轭物的合成需要衍生DNA寡核苷酸的5'或3'末端并共价连接该DNA与蛋白质。 可以使用抗生物素蛋白/链霉抗生物素蛋白 - 生物素连接，将DNA间接缀合至抗体或Fab'片段。 本发明的缀合物可用于免疫测定和PCR测定。

公开(公告)号：US5561043A

公开(公告)日：1996-10-01

申请号：US189448

申请日：1994-01-31

Applicant: Charles R. Cantor ,  Christof M. Niemeyer ,  Cassandra L. Smith ,  Takeshi Sano ,  Donald J. Hnatowich ,  Mary Rusckowski

Inventor： Charles R. Cantor ,  Christof M. Niemeyer ,  Cassandra L. Smith ,  Takeshi Sano ,  Donald J. Hnatowich ,  Mary Rusckowski

IPC: G01N33/566 ,  A61K38/00 ,  A61K38/16 ,  A61K38/21 ,  A61K38/22 ,  A61K38/27 ,  A61K38/43 ,  A61K39/00 ,  A61K39/395 ,  A61K45/00 ,  A61K47/48 ,  A61K49/00 ,  A61K51/00 ,  A61K51/12 ,  A61P31/04 ,  A61P31/12 ,  A61P35/00 ,  A61P35/02 ,  C03C17/30 ,  C03C17/34 ,  C07H21/04 ,  C12N15/09 ,  C12Q1/68 ,  C07H21/02

CPC classification number: B82Y5/00 ,  A61K47/481 ,  A61K47/48146 ,  A61K47/48961 ,  A61K51/1268 ,  C03C17/30 ,  C03C17/3405 ,  C12Q1/6804 ,  C12Q1/682 ,  Y10S977/80 ,  Y10S977/808 ,  Y10S977/898 ,  Y10S977/906 ,  Y10S977/92

Abstract: The invention is directed to constructs and compositions containing multimeric forms of nucleic acid. Multimeric nucleic acids comprise single-stranded nucleic acids attached via biotin to streptavidin and bound with a functional group. These constructs can be utilized in vivo to treat or identify diseased tissue or cells. Repeated administrations of multimeric nucleic acid compositions produce a rapid and specific amplification of nucleic acid constructs and their attached functional groups. For treatment purposes, functional groups may be toxins, radioisotopes, genes or enzymes. Diagnostically, labeled multimeric constructs may be used to identify specific targets in vivo or in vitro. Multimeric nucleic acids may also be used in nanotechnology and to create self-assembling polymeric aggregates such as membranes of defined porosity, microcircuits and many other products.

公开(公告)号：US5503980A

公开(公告)日：1996-04-02

申请号：US322526

申请日：1994-10-17

Applicant: Charles R. Cantor

Inventor： Charles R. Cantor

IPC: B01J19/00 ,  C12Q1/68 ,  C40B40/06 ,  C07H21/04 ,  C12P19/34

CPC classification number: B01J19/0046 ,  C12Q1/6837 ,  C40B80/00 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/0059 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00722 ,  C40B40/06

Abstract: This invention is directed to methods for determining a nucleotide sequence of a nucleic acid using positional sequencing by hybridization, and to the creation of nucleic acids probes which may be used with these methods. This invention is also directed to diagnostic aids for analyzing the nucleic acid composition and content of biological samples, including samples derived from medical and agricultural sources.

Abstract translation: 本发明涉及使用通过杂交的位置测序确定核酸的核苷酸序列的方法，以及可以与这些方法一起使用的核酸探针的产生。 本发明还涉及用于分析生物样品的核酸组成和含量的诊断助剂，包括衍生自医学和农业来源的样品。

公开(公告)号：US5482836A

公开(公告)日：1996-01-09

申请号：US4552

申请日：1993-01-14

Applicant: Charles R. Cantor ,  Takashi Ito ,  Cassandra L. Smith

Inventor： Charles R. Cantor ,  Takashi Ito ,  Cassandra L. Smith

IPC: C12N15/10 ,  C12Q1/68 ,  C07H17/00 ,  C12N15/00 ,  C12P19/34

CPC classification number: C12Q1/6839 ,  C12N15/1003 ,  C12N15/101 ,  C12Q1/6806

Abstract: The invention provides a method for purifying or isolating double stranded DNA intact using triple helix formation. The method includes the steps of complexing an oligonucleotide and double stranded DNA to generate a triple helix and immobilization of the triple helix on a solid phase by means of a molecular recognition system such as avidin/biotin. The purified DNA is then recovered intact by treating the solid phase with a reagent that breaks the bonds between the oligonucleotide and the intact double stranded DNA while not affecting the Watson-Crick base pairs of the double helix. The present invention also provides a method for purifying or isolating double stranded DNA intact by complexing the double stranded DNA with a specific binding partner and recovering the complex during electrophoresis by immobilizing it on a solid phase trap imbedded in an electrophoretic gel.

Abstract translation: 本发明提供了使用三螺旋形成来纯化或分离完整的双链DNA的方法。 该方法包括以下步骤：使寡核苷酸和双链DNA复合以产生三螺旋，并通过分子识别系统如抗生物素蛋白/生物素将固定三固定在固相上。 然后通过用破坏寡核苷酸和完整双链DNA之间的键的试剂处理固相，同时不影响双螺旋的Watson-Crick碱基对，完整地回收纯化的DNA。 本发明还提供了通过使双链DNA与特异性结合配偶体络合来纯化或分离双链DNA的方法，并通过将其固定在嵌入电泳凝胶中的固相捕集器上，在电泳过程中回收该配合物。

公开(公告)号：US5306619A

公开(公告)日：1994-04-26

申请号：US81070

申请日：1993-06-22

Applicant: Cynthia A. Edwards ,  Charles R. Cantor ,  Beth M. Andrews

Inventor： Cynthia A. Edwards ,  Charles R. Cantor ,  Beth M. Andrews

IPC: C07K14/47 ,  C12N15/09 ,  C12N15/10 ,  C12N15/67 ,  C12Q1/68 ,  C12Q1/70 ,  C07H17/00 ,  C12Q1/00 ,  G01N33/566

CPC classification number: C12N15/67 ,  C07K14/47 ,  C12N15/1048 ,  C12Q1/6811 ,  C12Q1/6883 ,  C12Q1/705

Abstract: The present invention defines a DNA:protein-binding assay useful for screening libraries of synthetic or biological compounds for their ability to bind DNA test sequences. The assay is versatile in that any number of test sequences can be tested by placing the test sequence adjacent to a defined protein binding screening sequence. Binding of molecules to these test sequence changes the binding characteristics of the protein molecule to its cognate binding sequence. When such a molecule binds the test sequence the equilibrium of the DNA:protein complexes is disturbed, generating changes in the concentration of free DNA probe. Also described herein is a method to capture DNA that has been released from the DNA:protein complex.

Abstract translation: 本发明定义了一种DNA：蛋白结合测定法，用于筛选合成或生物化合物文库结合DNA测试序列的能力。 该测定法是通用的，因为可以通过将测试序列置于与定义的蛋白质结合筛选序列相邻的位置来测试任何数量的测试序列。 分子与这些测试序列的结合改变了蛋白质分子与其同源结合序列的结合特征。 当这样的分子结合测试序列时，DNA：蛋白复合物的平衡受到干扰，产生游离DNA探针浓度的变化。 本文还描述了捕获已经从DNA：蛋白质复合物释放的DNA的方法。

公开(公告)号：US08304194B2

公开(公告)日：2012-11-06

申请号：US13223923

申请日：2011-09-01

Applicant: Charles R. Cantor ,  Chunming Ding

Inventor： Charles R. Cantor ,  Chunming Ding

IPC: C12Q1/68 ,  C12P19/34

CPC classification number: C12Q1/6851 ,  C12Q2545/107 ,  C12Q2535/125 ,  C12Q2525/186

Abstract: The present invention relates to a method for measuring the amount of a target nucleic acid in a sample using a standard which is designed to have one base difference compared with the gene of interest or a “target nucleic acid sequence.” Use of such standard in combination with a method of “enhancing” the difference in the standard and the test nucleic acid sample using, for example, a base extension reaction carried right at the mutation site allowing amplification of the standard and target nucleic acids with the same efficiency and facilitating quantification of the target nucleic acid. Thereafter a means of quantifying the “enhanced” standard and target nucleic acid samples is used to determine the amount of the target nucleic acid. In the preferred embodiment, the quantification means is Mass Spectrometry.

Abstract translation: 本发明涉及使用与目标基因或靶核酸序列相比设计为具有一个碱基差异的标准物来测量样品中靶核酸量的方法。 使用这种标准物与增加标准差和测试核酸样品的方法的方法结合使用例如在突变位点上携带的碱基延伸反应，允许以相同的效率扩增标准品和靶核酸 并促进靶核酸的定量。 此后，使用量化增强的标准和靶核酸样品的方法来确定靶核酸的量。 在优选实施方案中，定量方法是质谱法。

公开(公告)号：US20110244451A1

公开(公告)日：2011-10-06

申请号：US12844058

申请日：2010-07-27

Applicant: Charles R. Cantor ,  Chunming Ding

Inventor： Charles R. Cantor ,  Chunming Ding

IPC: C12Q1/68

CPC classification number: C12Q1/6883 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/6876 ,  C12Q2600/154 ,  C12Q2600/156 ,  C12Q2600/166 ,  C12Q2521/331

Abstract: Chromosomal abnormalities are responsible for a significant number of birth defects, including mental retardation. The present invention is related to methods for non-invasive and rapid, prenatal diagnosis of chromosomal abnormalities based on analysis of a maternal blood sample. The invention exploits the differences in DNA between the mother and fetus, for instance differences in their methylation states, as a means to enrich for fetal DNA in maternal plasma sample. The methods described herein can be used to detect chromosomal DNA deletions and duplications. In a preferred embodiment, the methods are used to diagnose chromosomal aneuploidy and related disorders, such as Down's and Turner's Syndrome.

Abstract translation: 染色体异常负责大量的出生缺陷，包括精神发育迟滞。 本发明涉及基于母体血液样品分析的非侵入性和快速，产前诊断染色体异常的方法。 本发明利用母体和胎儿之间的DNA差异，例如甲基化状态的差异，作为富集母体血浆样品中胎儿DNA的手段。 本文描述的方法可用于检测染色体DNA缺失和重复。 在优选的实施方案中，所述方法用于诊断染色体非整倍体和相关疾病，例如唐氏和特纳综合征。

公开(公告)号：US20110124518A1

公开(公告)日：2011-05-26

申请号：US12863169

申请日：2009-01-16

Applicant: Charles R. Cantor

Inventor： Charles R. Cantor

IPC: C40B30/04 ,  C12Q1/68 ,  B82Y15/00

CPC classification number: C12Q1/6818 ,  C12Q1/6816 ,  C12Q1/6869 ,  C12Q2521/501 ,  C12Q2525/161 ,  C12Q2563/185

Abstract: Provided herein are compositions and methods for analysis of nucleic acids, including, methods and compositions for genotyping, haplotyping, sequencing and performing other genetic and epigenetic analyses on nucleic acids, for example. In some embodiments, methods and compositions suitable for whole-genome sequencing on single molecules of nucleic acid are provided. In some embodiments, analysis of single molecules of nucleic acid are performed in conjunction with nanopores and/or nanopore devices.

Abstract translation: 本文提供了用于分析核酸的组合物和方法，包括用于例如基因分型，单倍型，测序和对核酸进行其它遗传和表观遗传学分析的方法和组合物。 在一些实施方案中，提供适用于单分子核酸上的全基因组测序的方法和组合物。 在一些实施方案中，结合纳米孔和/或纳米孔装置进行单分子核酸的分析。