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    Methods for reducing the harshness of a cotton-containing fabric
    42.
    发明授权
    Methods for reducing the harshness of a cotton-containing fabric 失效
    减少含棉织物粗糙度的方法

    公开(公告)号：US5648263A

    公开(公告)日：1997-07-15

    申请号：US453606

    申请日：1995-05-30

    申请人： Martin Schulein ,  Kirsten B.o slashed.egh Levring

    发明人： Martin Schulein ,  Kirsten B.o slashed.egh Levring

    IPC分类号： C11D3/386 ,  C12N9/42 ,  D06M16/00 ,  C11D7/42

    CPC分类号： C11D3/38645 ,  C12N9/2437 ,  C12Y302/01004 ,  D06M16/003 ,  D06M2101/06 ,  D06M2200/50 ,  Y10S435/911 ,  Y10S435/929

    摘要： The present invention relates to methods of reducing the rate at which a cotton-containing fabric becomes harsh or of reducing the harshness of a cotton-containing fabric, comprising treating the cotton-containing fabric with a cellulase preparation obtained from a fungus, wherein the cellulase preparation comprises a substantially homogeneous endoglucanase component which: (a) has a pH optimum of about 7.5-10.0; (b) exhibits a CMC endoase activity of at least about 10 CMC-endoase units per mg of total protein; (c) exhibits a CAVU activity of at least about 50% at a pH of 7.0-12.0; and (d) has essentially no cellobiohydrolase activity; wherein at least about 50% by weight of the total cellulase content of the cellulase preparation is the endoglucanase component.

    摘要翻译： 本发明涉及降低含棉织物变得苛刻或降低含棉织物粗糙度的速率的方法，包括用由真菌获得的纤维素酶制剂处理含棉织物，其中纤维素酶 制备物包含基本均匀的内切葡聚糖酶组分，其中：（a）pH最佳值为约7.5-10.0; （b）表现出每mg总蛋白质至少约10个CMC-内切酶单位的CMC内切酶活性; （c）在7.0-12.0的pH下表现出至少约50％的CAVU活性; 和（d）基本上没有纤维二糖水解酶活性; 其中至少约50重量％的纤维素酶制剂的总纤维素酶含量是内切葡聚糖酶组分。

    Endoglucanases
    46.
    发明申请
    Endoglucanases 审中-公开

    公开(公告)号：US20080145912A1

    公开(公告)日：2008-06-19

    申请号：US11740076

    申请日：2007-04-25

    申请人： Martin Schulein ,  Torben Henriksen ,  Lene Nonboe Andersen ,  Soren Flensted Lassen ,  Markus Sakari Kauppinen ,  Lene Lange ,  Ruby Ilum Nielsen ,  Shinobu Takagi ,  Michiko Ihara

    发明人： Martin Schulein ,  Torben Henriksen ,  Lene Nonboe Andersen ,  Soren Flensted Lassen ,  Markus Sakari Kauppinen ,  Lene Lange ,  Ruby Ilum Nielsen ,  Shinobu Takagi ,  Michiko Ihara

    IPC分类号： C12N9/18 ,  C07H21/04 ,  C12N15/00 ,  D06M16/00

    摘要： The present invention relates to enzyme preparations consisting essentially of an enzyme which has cellulytic activity and comprises a first amino acid sequence having the following sequence (SEQ ID NO: 79) Thr Arg Xaa Xaa Asp Cys Cys Xaa Xaa Xaa Cys Xaa 1   2   3   4   5   6   7   8   9   10  11  12 Trp Xaa 13  14 and a second amino acid sequence having the following sequence Trp Cys Cys Xaa Cys(SEQ ID NO: 80) 1   2   3   4   5 wherein, at position 3 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 4 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 8 of the first sequence, the amino acid is Arg, Lys or His; at positions 9, 10, 12 and 14, respectively, of the first sequence, and at position 4 of the second sequence, the amino acid is any of the 20 naturally occurring amino acid residues with the provisos that, in the first amino acid sequence, (i) when the amino residue at position 12 is Ser, then the amino acid residue at position 14 is not Ser, and (ii) when the amino residue at position 12 is Gly, then the amino acid residue at position 14 is not Ala, performs very well in industrial applications such as laundry compositions, for biopolishing of newly manufactured textiles, for providing an abraded look of cellulosic fabric or garment, and for treatment of paper pulp. Further, the invention relates to DNA constructs encoding such enzymes, a method for providing a gene encoding for such enzymes, a method of producing the enzymes, enzyme preparations containing such enzymes, and the use of these enzymes for a number of industrial applications.

    Mannanases
    47.
    发明授权
    Mannanases 有权
    甘露聚糖酶

    公开(公告)号：US07183093B2

    公开(公告)日：2007-02-27

    申请号：US10372054

    申请日：2003-02-20

    申请人： Markus Sakari Kauppinen ,  Martin Schulein ,  Kirk Schnorr ,  Lene Nonboe Andersen ,  Mads Eskelund Bjornvad

    发明人： Markus Sakari Kauppinen ,  Martin Schulein ,  Kirk Schnorr ,  Lene Nonboe Andersen ,  Mads Eskelund Bjornvad

    IPC分类号： C12N9/42 ,  C12N1/20 ,  C12P19/64 ,  C12P19/34 ,  C12P19/04 ,  D06M16/00

    CPC分类号： C12N9/2491 ,  C12Y302/01025 ,  C12Y302/01078

    摘要： The present invention relates to mannanases comprising e.g., a sequence of amino acids 32–330 of SEQ ID NO: 2 or their homologues may be derived from e.g., Bacillus sp. I633, or may be encoded by polynucleotide molecules comprising a sequence of nucleotides 94–990 of SEQ ID NO: 1 from, polynucleotide molecules that encode a polypeptide that is at least 65% identical to the sequence of amino acids 32–330 of SEQ ID NO: 2, or degenerate nucleotide sequences thereof. The mannanases are alkaline and are useful e.g. in cleaning compositions, in a fracturing fluid useful to fracture a subterranean formation, for modifying plant material, and for treatment of cellulosic fibers.

    摘要翻译： 本发明涉及包含例如SEQ ID NO：2的氨基酸32-330的序列的甘露聚糖酶或其同源物可以衍生自例如Bacillus sp。 或者可以由包含SEQ ID NO：1的核苷酸94-990的序列的多核苷酸分子编码的多核苷酸分子编码多肽分子，所述多核苷酸分子编码与SEQ ID NO：32的氨基酸32-330的序列至少65％相同的多肽 NO：2或其简并核苷酸序列。 甘露聚糖酶是碱性的，并且是有用的。 在清洁组合物中，在用于破坏地层的压裂液中，用于修饰植物材料和用于纤维素纤维的处理。

    Endo-beta-1,4-glucanases
    49.
    发明申请
    Endo-beta-1,4-glucanases 有权
    内-β-1,4-葡聚糖酶

    公开(公告)号：US20050215450A1

    公开(公告)日：2005-09-29

    申请号：US11044363

    申请日：2005-01-26

    申请人： Helle Outtrup ,  Martin Schulein ,  Torben Henriksen ,  Mads Bjornvad ,  Keith Gibson

    发明人： Helle Outtrup ,  Martin Schulein ,  Torben Henriksen ,  Mads Bjornvad ,  Keith Gibson

    IPC分类号： C11D3/386 ,  C07H21/04 ,  C12N1/21 ,  C12N9/42 ,  C12N15/74 ,  C12Q1/68

    CPC分类号： C11D3/386

    摘要： The present invention relates to an enzyme exhibiting endo-beta-1,4-glucanase activity (EC 3.2.1.4), which is a) a polypeptide encoded by the DNA sequence of positions 1 to 2322 of SEQ ID NO: 1; b) a polypeptide produced by culturing a cell comprising the sequence of SEQ ID NO: 1 under conditions wherein the DNA sequence is expressed; c) an endo-beta-1,4-glucanase enzyme having a sequence of at least 97% identity to the amino acid sequence of position 1 to position 773 of SEQ ID NO: 2; and fragments thereof exhibiting endo-beta-1,4-glucanase activity, and d) a polypeptide having endo-beta-1,4-glucanase activity that is encoded by a polynucleotide that hybridizes with the nucleotide sequence shown in positions 1-2322 of SEQ ID NO: 1, is useful for detergent and textile applications.

    摘要翻译： 本发明涉及显示内β-1,4-葡聚糖酶活性的酶（EC 3.2.1.4），其是a）由SEQ ID NO：1的1至2322位的DNA序列编码的多肽; b）通过在表达DNA序列的条件下培养包含SEQ ID NO：1的序列的细胞产生的多肽; c）具有与SEQ ID NO：2的位置1至位置773的氨基酸序列具有至少97％同一性的序列的内切-β-1,4-葡聚糖酶; 和表现出内切-β-1,4-葡聚糖酶活性的片段，以及d）具有内切-β-1,4-葡聚糖酶活性的多肽，其由与SEQ ID NO：1-2322所示核苷酸序列杂交的多核苷酸编码 SEQ ID NO：1可用于洗涤剂和纺织品应用。

    Endoglucanases
    50.
    发明申请
    Endoglucanases 有权
    内切葡聚糖酶

    公开(公告)号：US20050070003A1

    公开(公告)日：2005-03-31

    申请号：US10965499

    申请日：2004-10-14

    申请人： Martin Schulein ,  Torben Henriksen ,  Lene Andersen ,  Soren Lassen ,  Markus Kauppinen ,  Lene Lange ,  Ruby Nielsen ,  Shinobu Takagi ,  Michiko Ihara

    发明人： Martin Schulein ,  Torben Henriksen ,  Lene Andersen ,  Soren Lassen ,  Markus Kauppinen ,  Lene Lange ,  Ruby Nielsen ,  Shinobu Takagi ,  Michiko Ihara

    IPC分类号： C12N15/09 ,  C05F11/08 ,  C05F17/00 ,  C07G99/00 ,  C11D3/386 ,  C12N1/19 ,  C12N9/42 ,  C12N15/00 ,  C12R1/645 ,  C12R1/69 ,  C12R1/77 ,  C12R1/80 ,  C12S3/04 ,  C12S11/00 ,  D06M13/02 ,  D06M13/322 ,  D06M13/46 ,  D06M16/00 ,  D06P5/02 ,  D06P5/15 ,  D21C5/00 ,  D21H11/16 ,  D21H11/20 ,  D21H17/00 ,  D21H21/10

    CPC分类号： C12N9/2437 ,  A61K38/00 ,  C05F11/08 ,  C05F17/0036 ,  C07K2319/00 ,  C11D3/386 ,  C12P19/14 ,  C12Y302/01004 ,  D06M16/003 ,  D06P5/02 ,  D06P5/158 ,  D21C5/005 ,  D21H17/005 ,  D21H21/10 ,  Y02P20/145 ,  Y02W30/43

    摘要： The present invention relates to enzyme preparations consisting essentially of an enzyme which has cellulytic activity and comprises a first amino acid sequence having the following sequence (SEQ ID NO: 79) ThrArgXaaXaaAspCysCysXaaXaaXaaCysXaaTrpXaa 1234567891011121314 and a second amino acid sequence having the following sequence TrpCysCysXaaCys(SEQ ID NO: 80) 12345 wherein, at position 3 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 4 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 8 of the first sequence, the amino acid is Arg, Lys or His; at positions 9, 10, 12 and 14, respectively, of the first sequence, and at position 4 of the second sequence, the amino acid is any of the 20 naturally occurring amino acid residues with the provisos that, in the first amino acid sequence, (i) when the amino residue at position 12 is Ser, then the amino acid residue at position 14 is not Ser, and (ii) when the amino residue at position 12 is Gly, then the amino acid residue at position 14 is not Ala, performs very well in industrial applications such as laundry compositions, for biopolishing of newly manufactured textiles, for providing an abraded look of cellulosic fabric or garment, and for treatment of paper pulp. Further, the invention relates to DNA constructs encoding such enzymes, a method for providing a gene encoding for such enzymes, a method of producing the enzymes, enzyme preparations containing such enzymes, and the use of these enzymes for a number of industrial applications.

    摘要翻译： 本发明涉及酶制剂，其基本上由具有纤维素分解活性的酶组成，并且包含具有以下序列的第一氨基酸序列和具有以下序列的第二个氨基酸序列，其中在第一序列的3位是氨基酸 是Trp，Tyr或Phe; 在第一序列的位置4处，氨基酸是Trp，Tyr或Phe; 在第一序列的位置8，氨基酸是Arg，Lys或His; 在第一序列的第9,10,12和14位，第2个序列的第4位，氨基酸是20个天然存在的氨基酸残基中的任一个，条件是在第一个氨基酸序列 ，（i）当第12位的氨基残基为Ser时，第14位的氨基酸残基不为Ser，（ii）当第12位的氨基残基为Gly时，第14位的氨基酸残基为 Ala在工业应用中表现良好，例如洗衣组合物，用于生物抛光新制造的纺织品，用于提供纤维素织物或衣服的磨损外观以及纸浆处理。 此外，本发明涉及编码这些酶的DNA构建体，提供编码这种酶的基因的方法，生产酶的方法，含有这些酶的酶制剂，以及这些酶用于许多工业应用的用途。