公开(公告)号：US20090305909A1

公开(公告)日：2009-12-10

申请号：US12482815

申请日：2009-06-11

Applicant: Eric S. Nordman ,  Mark F. Oldham ,  Timothy Woudenberg

Inventor： Eric S. Nordman ,  Mark F. Oldham ,  Timothy Woudenberg

IPC: C40B50/18 ,  C40B50/14

CPC classification number: C12Q1/6874 ,  C12Q1/6869 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/648 ,  G01N21/6486 ,  G01N2021/6441 ,  C12Q2563/107

Abstract: A method for selectively immobilizing a molecule of interest on a substrate is disclosed. A substrate having a first surface component and a second surface component is provided. The first and second surface components have different surface characteristics. The molecule of interest is selectively coupled to the first surface component based upon a difference between the surface characteristics of the first surface component and the surface characteristics of the second surface component.

Abstract translation: 公开了一种用于选择性地将感兴趣的分子固定在基底上的方法。 提供了具有第一表面部件和第二表面部件的基板。 第一和第二表面部件具有不同的表面特性。 基于第一表面成分的表面特性和第二表面成分的表面特性之间的差异，感兴趣的分子选择性地耦合到第一表面成分。

公开(公告)号：US20090186779A1

公开(公告)日：2009-07-23

申请号：US12417855

申请日：2009-04-03

Applicant: Timothy Z. Liu ,  Timothy Woudenberg ,  Jer-Kang Chen

Inventor： Timothy Z. Liu ,  Timothy Woudenberg ,  Jer-Kang Chen

IPC: C40B40/06 ,  C40B40/04 ,  C40B40/10 ,  C40B40/12 ,  C40B50/18

CPC classification number: B01J19/0046 ,  B01J2219/00527 ,  B01J2219/00596 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00722 ,  C40B40/06

Abstract: An array comprising a probe covalently attached to a diamond substrate is fabricated, for example, by treating the diamond substrate with an aryl diazonium compound and covalently attaching the probe to the aryl group. Some embodiments are useful in DNA-based sensing applications.

Abstract translation: 包含共价连接到金刚石基底的探针的阵列例如通过用芳基重氮化合物处理金刚石基底并将探针共价连接到芳基来制备。 一些实施方案在基于DNA的感测应用中是有用的。

公开(公告)号：US20070134710A1

公开(公告)日：2007-06-14

申请号：US11651317

申请日：2007-01-09

Applicant: Timothy Woudenberg ,  Michael Albin ,  Reid Kowallis ,  Yefim Raysberg ,  Robert Ragusa ,  Emily Winn-Deen

Inventor： Timothy Woudenberg ,  Michael Albin ,  Reid Kowallis ,  Yefim Raysberg ,  Robert Ragusa ,  Emily Winn-Deen

IPC: C12Q1/68 ,  C07H21/04 ,  C12M3/00

CPC classification number: C12Q1/6816 ,  B01J2219/00317 ,  B01J2219/00599 ,  B01J2219/00722 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/50851 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0642 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/1827 ,  B01L2400/049 ,  C12Q1/08 ,  C40B40/06 ,  C40B50/08 ,  C40B60/14 ,  G01N21/6452 ,  G01N2035/00148 ,  Y10S436/805 ,  Y10S436/807 ,  Y10S436/808 ,  Y10S436/809 ,  Y10T436/143333 ,  Y10T436/25 ,  C12Q2565/631

Abstract: The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. The device includes a substrate which defines a sample-distribution network having (i) a sample inlet, (ii) one or more detection chambers, and (iii) channel means providing a dead-end fluid connection between each of the chambers and the inlet Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.

Abstract translation: 本发明涉及一种用于同时测试样品的一种或多种选择的分析物的存在，不存在和/或量的方法和装置。 本发明在一个方面包括用于检测或定量液体样品中的多种不同分析物的装置。 该装置包括限定样品分配网络的基板，其具有（i）样品入口，（ii）一个或多个检测室，以及（iii）通道装置，其在每个室之间提供死端流体连接，并且入口 每个室可以包括有效地与可能存在于样品中的选定分析物反应的分析物特异性试剂和用于检测信号的检测装置。 还公开了利用该装置的方法。

公开(公告)号：US20060286659A1

公开(公告)日：2006-12-21

申请号：US11433872

申请日：2006-05-12

Applicant: John Atwood ,  Albert Mossa ,  Lisa Goven ,  Fenton Williams ,  Timothy Woudenberg ,  Marcel Margulies ,  Robert Ragusa ,  Richard Leath ,  Clive Miles

Inventor： John Atwood ,  Albert Mossa ,  Lisa Goven ,  Fenton Williams ,  Timothy Woudenberg ,  Marcel Margulies ,  Robert Ragusa ,  Richard Leath ,  Clive Miles

IPC: C12M1/38

CPC classification number: B01L3/50851 ,  B01L3/50853 ,  B01L7/00 ,  B01L7/52 ,  B01L2200/147 ,  B01L2300/042 ,  B01L2300/046 ,  B01L2300/0829 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/185 ,  G05D23/1917 ,  Y10S435/809

Abstract: A thermal cycler for automatic performance of the polymerase chain reaction is provided. The thermal cycler comprises a heater control that provides close temperature control of the reaction.

公开(公告)号：US20060210439A1

公开(公告)日：2006-09-21

申请号：US11434737

申请日：2006-05-16

Applicant: Timothy Woudenberg ,  Michael Albin ,  Reid Kowallis ,  Yefim Raysberg ,  Robert Ragusa ,  Emily Winn-Deen

Inventor： Timothy Woudenberg ,  Michael Albin ,  Reid Kowallis ,  Yefim Raysberg ,  Robert Ragusa ,  Emily Winn-Deen

IPC: B32B27/04 ,  G01N31/00

CPC classification number: C12Q1/6816 ,  B01J2219/00317 ,  B01J2219/00599 ,  B01J2219/00722 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/50851 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0642 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/1827 ,  B01L2400/049 ,  C12Q1/08 ,  C40B40/06 ,  C40B50/08 ,  C40B60/14 ,  G01N21/6452 ,  G01N2035/00148 ,  Y10S436/805 ,  Y10S436/807 ,  Y10S436/808 ,  Y10S436/809 ,  Y10T436/143333 ,  Y10T436/25 ,  C12Q2565/631

Abstract: The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more of a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. The device can comprise wells and each well can comprise an analyte-specific reagent effective to react with a selected analyte that may be present in the sample. Also disclosed are methods utilizing the device.

Abstract translation: 本发明涉及一种用于同时测试样品中多种所选分析物中一种或多种的存在，不存在和/或量的方法和装置。 本发明在一个方面包括用于检测或定量液体样品中的多种不同分析物的装置。 该装置可以包括孔，并且每个孔可以包含有效地与可能存在于样品中的选定分析物反应的分析物特异性试剂。 还公开了利用该装置的方法。

公开(公告)号：US20050112634A1

公开(公告)日：2005-05-26

申请号：US10944686

申请日：2004-09-17

Applicant: Timothy Woudenberg ,  Robert Jones ,  Kenneth Livak

Inventor： Timothy Woudenberg ,  Robert Jones ,  Kenneth Livak

IPC: C12N20060101 ,  C12P19/34 ,  C12Q1/68 ,  G01N20060101

CPC classification number: B01L3/50851 ,  B01L3/50853 ,  B01L3/50857 ,  B01L2200/0642 ,  B01L2300/044 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2400/0409 ,  B01L2400/0487 ,  C12Q1/6837

Abstract: Methods for amplifying polynucleotides, e.g., by PCR, in a sample comprising polynucleotide targets present at very low concentration, comprising: (a) applying amplification reactants to the surface of a substrate comprising reaction spots, wherein the reactants comprise the sample and an amplification reagent; (b) forming a sealed reaction chamber, having a volume less than about 120 nanoliters, preferably less than about 20 nanoliters, over each of said reaction spots; and (c) thermal cycling the substrate and reactants. In one embodiment, the forming step comprises loading a sealing fluid, e.g., mineral oil, on the surface so as to cover the reaction spots. The present invention also provides microplates, comprising: (a) a substrate having at least about 10,000 reaction spots, each comprising a primer and a droplet of reagent having a volume less than about 120 nanoliters, preferably less then about 20 nanoliters; and (b) a sealing liquid isolating each of the spots.

Abstract translation: 在包含以非常低浓度存在的多核苷酸靶标的样品中通过PCR扩增多核苷酸的方法包括：（a）将扩增反应物施加到包含反应点的底物表面，其中反应物包含样品和扩增试剂 ; （b）在每个所述反应点上形成体积小于约120纳升，优选小于约20纳升的密封反应室; 和（c）使底物和反应物热循环。 在一个实施例中，成形步骤包括在表面上装载密封流体，例如矿物油，以便覆盖反应点。 本发明还提供了微孔板，其包括：（a）具有至少约10,000个反应点的底物，每个底物包含体积小于约120纳升，优选小于约20纳升的底漆和试剂滴; 和（b）隔离每个斑点的密封液体。