公开(公告)号：US20070026476A1

公开(公告)日：2007-02-01

申请号：US11460366

申请日：2006-07-27

申请人： Dieter Heindl ,  Joachim Hoenes ,  Carina Horn ,  Piet Herdewijn

发明人： Dieter Heindl ,  Joachim Hoenes ,  Carina Horn ,  Piet Herdewijn

IPC分类号： C12Q1/54 ,  C07H19/04

CPC分类号： C07D405/14 ,  C07D403/12 ,  C07D403/14 ,  C07H19/04 ,  C07H19/167 ,  C12Q1/32 ,  C12Q1/54 ,  G01N2333/90209

摘要： The invention concerns stable nicotinamide adenine dinucleotide (NAD/NADH) and nicotinamide adenine dinucleotide phosphate (NADP/NADPH) derivatives, enzyme complexes of these derivatives and their use in biochemical detection methods and reagent matrices.

摘要翻译： 本发明涉及稳定的烟酰胺腺嘌呤二核苷酸（NAD / NADH）和烟酰胺腺嘌呤二核苷酸磷酸（NADP / NADPH）衍生物，这些衍生物的酶复合物及其在生化检测方法和试剂基质中的应用。

公开(公告)号：US07122355B2

公开(公告)日：2006-10-17

申请号：US10192902

申请日：2002-07-11

申请人： Waltraud Ankenbauer ,  Dieter Heindl ,  Frank Laue ,  Andreas Huber

发明人： Waltraud Ankenbauer ,  Dieter Heindl ,  Frank Laue ,  Andreas Huber

IPC分类号： C12P19/34

CPC分类号： C12Q1/6848 ,  C12Q1/6853 ,  C12Q2549/101 ,  C12Q2525/186 ,  C12Q2521/319

摘要： The present invention is directed to a new composition for performing a nucleic acid amplification reaction comprising (i) a thermostable DNA-Polymerase, (ii) a thermostable 3′-5′ Exonuclease, and (iii) at least one primer for nucleic acid amplification with a modified 3′ terminal residue which is not elongated by said thermostable DNA-Polymerase as well as methods for performing a PCR reaction using this composition. Furthermore, the method is directed to kits comprising such a composition.

公开(公告)号：US06875850B2

公开(公告)日：2005-04-05

申请号：US09943411

申请日：2001-08-30

申请人： Dieter Heindl ,  Gregor Sagner ,  Heribert Maerz ,  Herbert Von Der Eltz

发明人： Dieter Heindl ,  Gregor Sagner ,  Heribert Maerz ,  Herbert Von Der Eltz

IPC分类号： G01N33/58 ,  C07B61/00 ,  C07C217/10 ,  C07C233/18 ,  C07H21/00 ,  C07H21/04 ,  C08G69/08 ,  C09B11/28 ,  C09B69/10 ,  C12N15/09 ,  C07H19/00 ,  C12Q1/68

CPC分类号： C07H21/00 ,  C07B2200/11 ,  C40B40/00 ,  Y02P20/55 ,  Y10T428/2996

摘要： The present invention concerns a labelling reagent in which the label is bound via an amide bond and a linker to a residue of the molecule which is essentially characterized in that the N atom of the amide bond and the label are linked together directly by a covalent bond. In particular these are phosphoramidites or reactive supports suitable for nucleic acid synthesis. The invention also concerns processes for the production of such supports from suitable precursors.

摘要翻译： 本发明涉及一种标记试剂，其中标记通过酰胺键和接头连接至分子的残基，其基本特征在于酰胺键的N原子和标记直接通过共价键连接在一起 。 特别地，这些是适合于核酸合成的亚磷酰胺或反应性载体。 本发明还涉及从合适的前体制备这种载体的方法。

公开(公告)号：US10260085B2

公开(公告)日：2019-04-16

申请号：US13524199

申请日：2012-06-15

申请人： Carina Horn ,  Dieter Heindl ,  Hans-Peter Haar ,  Nelli Steinke

发明人： Carina Horn ,  Dieter Heindl ,  Hans-Peter Haar ,  Nelli Steinke

IPC分类号： C12Q1/54

摘要： In one form, a diagnostic element for determining at least one analyte is provided. In a further form, an analytical measuring device includes the diagnostic element. Still, other forms are related to methods for the determination of an analyte, correcting a signal generated by an analyte, and/or checking the detection optics of an analytical measuring device using the diagnostic element. Another form is related to a system for the controlled release of a reagent and the use of such a system as a circuit element. Other aspects include, but are not limited to, unique methods, techniques, products, systems and devices involving diagnostic elements.

公开(公告)号：US20130190191A1

公开(公告)日：2013-07-25

申请号：US13746812

申请日：2013-01-22

申请人： Thomas Froehlich ,  Dieter Heindl ,  Angelika Roesler

发明人： Thomas Froehlich ,  Dieter Heindl ,  Angelika Roesler

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6874 ,  C12Q1/6834 ,  C12Q1/6846 ,  C12Q2563/149 ,  C12Q2525/197 ,  C12Q2523/319 ,  C12Q2565/537 ,  C12Q2547/101 ,  C12Q2537/143

摘要： The present invention is directed to method for analyzing multiple nucleic acid molecules of interest comprising in the steps of (i) providing a plurality of beads, characterized in that each bead comprises at least two sequence specific amplification primers, further characterized in that at least one of the primers is bound to the bead via a cleavable linker, (ii) capturing the nucleic acid molecules of interest from a sample, (iii) clonally isolating the plurality of beads, (iv) cleaving the at least one primer, (v) clonally amplifying the nucleic acid thereby creating multiple amplification products, and (vi) analyzing the amplification products.

摘要翻译： 本发明涉及用于分析感兴趣的多个核酸分子的方法，包括以下步骤：（i）提供多个珠粒，其特征在于每个珠粒包含至少两个序列特异性扩增引物，其特征还在于至少一个 的引物通过可切割的接头与珠结合，（ii）从样品中捕获感兴趣的核酸分子，（iii）克隆分离多个珠，（iv）切割至少一个引物，（v） 克隆扩增核酸从而产生多个扩增产物，和（vi）分析扩增产物。

公开(公告)号：US08357490B2

公开(公告)日：2013-01-22

申请号：US12841708

申请日：2010-07-22

申请人： Thomas Froehlich ,  Martin Gutekunst ,  Dieter Heindl ,  Angelika Roesler ,  Rudolf Seibl

发明人： Thomas Froehlich ,  Martin Gutekunst ,  Dieter Heindl ,  Angelika Roesler ,  Rudolf Seibl

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C40B60/02 ,  C40B60/04 ,  C40B60/08

CPC分类号： G01N35/028 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00308 ,  B01J2219/00315 ,  B01J2219/00364 ,  B01J2219/00423 ,  B01J2219/00454 ,  B01J2219/00511 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00639 ,  B01J2219/00691 ,  B01J2219/00695 ,  B01J2219/00722 ,  B01L7/52 ,  C40B60/14 ,  G01N35/1002 ,  G01N2035/00326 ,  G01N2035/00356

摘要： An integrated instrument for oligonucleotide synthesis and PCR, and a system and method thereof are disclosed. The integrated instrument is basically composed of two independent modules. The first module is a unit for chemical de novo synthesis of oligonucleotides such as oligonucleotide primers and/or oligonucleotide hybridization probes. The second module is a unit for performing an analytical polymerase chain reaction amplification in real time, i.e. a qPCR. The two modules are operatively linked to each other in such a way that a user can load a nucleic sample to be analyzed into the integrated instrument and perform a PCR reaction by programming the instrument without a previous external synthesis of oligonucleotide amplification primers.

摘要翻译： 公开了一种用于寡核苷酸合成和PCR的综合仪器及其系统及方法。 综合仪器基本上由两个独立的模块组成。 第一模块是寡核苷酸引物和/或寡核苷酸杂交探针等寡核苷酸的化学从头合成的单元。 第二个模块是用于实时进行分析聚合酶链反应扩增的单元，即qPCR。 两个模块可操作地彼此连接，使得用户可以将待分析的核酸样品加载到整合的仪器中，并且通过在没有先前的外部合成寡核苷酸扩增引物的情况下编程仪器进行PCR反应。

公开(公告)号：US20120130062A1

公开(公告)日：2012-05-24

申请号：US13358146

申请日：2012-01-25

申请人： Hartmut Duefel ,  Dieter Heindl ,  Carina Horn ,  Thomas Meier ,  Rainer Schmuck

发明人： Hartmut Duefel ,  Dieter Heindl ,  Carina Horn ,  Thomas Meier ,  Rainer Schmuck

IPC分类号： C07H19/207 ,  C12P19/32

CPC分类号： C07H19/207 ,  C12P19/32

摘要： The disclosure concerns the enzymatic synthesis of stable analogues of nicotinamide adenine dinucleotide NAD/NADH and nicotinamide adenine dinucleotide phosphate NADP/NADPH, the so-called “carba-NADs”, i.e. analogues of NAD/NADH or NADP/NADPH, respectively, comprising a carbacyclic sugar instead of ribose.

摘要翻译： 本公开内容涉及烟酰胺腺嘌呤二核苷酸NAD / NADH和烟酰胺腺嘌呤二核苷酸磷酸NADP / NADPH的稳定类似物的酶合成，即所谓的“carba-NAD”，即NAD / NADH或NADP / NADPH的类似物，分别包含 碳环糖代替核糖。

公开(公告)号：US08153779B2

公开(公告)日：2012-04-10

申请号：US12580445

申请日：2009-10-16

申请人： Dieter Heindl

发明人： Dieter Heindl

IPC分类号： C07H19/10 ,  C07H19/20

CPC分类号： C07H19/10 ,  C07H19/20 ,  C07H21/00

摘要： Described are modified mononucleotides and processes for their production. The nucleotides have a structure in which B is a purine or pyrimidine, S is a sugar unit, Y is an OH, a monophosphate, or a diphosphate, and Acc is an electron acceptor selected from the group consisting of (a) —CN and (b) —SO2—R′ in which R′ contains one amino-substituted alkyl or one optionally substituted aryl.

摘要翻译： 描述的是修饰的单核苷酸及其生产方法。 核苷酸具有其中B是嘌呤或嘧啶，S是糖单元，Y是OH，单磷酸酯或二磷酸酯的结构，并且Acc是选自（a）-CN和 （b）-SO 2 -R'，其中R'含有一个氨基取代的烷基或一个任选取代的芳基。

公开(公告)号：US20110177514A1

公开(公告)日：2011-07-21

申请号：US12841708

申请日：2010-07-22

申请人： Thomas Froehlich ,  Martin Gutekunst ,  Dieter Heindl ,  Angelika Roesler ,  Rudolf Seibl

发明人： Thomas Froehlich ,  Martin Gutekunst ,  Dieter Heindl ,  Angelika Roesler ,  Rudolf Seibl

IPC分类号： C12Q1/68 ,  C12M1/00 ,  C40B60/14 ,  C12M1/34

CPC分类号： G01N35/028 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00308 ,  B01J2219/00315 ,  B01J2219/00364 ,  B01J2219/00423 ,  B01J2219/00454 ,  B01J2219/00511 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00639 ,  B01J2219/00691 ,  B01J2219/00695 ,  B01J2219/00722 ,  B01L7/52 ,  C40B60/14 ,  G01N35/1002 ,  G01N2035/00326 ,  G01N2035/00356

摘要： An integrated instrument for oligonucleotide synthesis and PCR, and a system and method thereof are disclosed. The integrated instrument is basically composed of two independent modules. The first module is a unit for chemical de novo synthesis of oligonucleotides such as oligonucleotide primers and/or oligonucleotide hybridization probes. The second module is a unit for performing an analytical polymerase chain reaction amplification in real time, i.e. a qPCR. The two modules are operatively linked to each other in such a way that a user can load a nucleic sample to be analyzed into the integrated instrument and perform a PCR reaction by programming the instrument without a previous external synthesis of oligonucleotide amplification primers.

摘要翻译： 公开了一种用于寡核苷酸合成和PCR的综合仪器及其系统及方法。 综合仪器基本上由两个独立的模块组成。 第一模块是寡核苷酸引物和/或寡核苷酸杂交探针等寡核苷酸的化学从头合成的单元。 第二个模块是用于实时进行分析聚合酶链反应扩增的单元，即qPCR。 两个模块可操作地彼此连接，使得用户可以将待分析的核酸样品加载到整合的仪器中，并且通过在没有先前的外部合成寡核苷酸扩增引物的情况下编程仪器进行PCR反应。

公开(公告)号：US20110143416A1

公开(公告)日：2011-06-16

申请号：US12859654

申请日：2010-08-19

申请人： Carina Horn ,  Dieter Heindl ,  Claudia Gaessler-Dietsche ,  Joachim Hoenes

发明人： Carina Horn ,  Dieter Heindl ,  Claudia Gaessler-Dietsche ,  Joachim Hoenes

IPC分类号： C12N9/96

CPC分类号： C12N9/0006 ,  C12N9/96

摘要： The present invention relates to a method for stabilizing an enzyme by storing the enzyme in the presence of a stable coenzyme. The present invention further relates to an enzyme stabilized with a stable coenzyme, and to the use thereof in test elements for detecting analytes.

摘要翻译： 本发明涉及通过在稳定的辅酶的存在下储存酶来稳定酶的方法。 本发明还涉及用稳定的辅酶稳定的酶，以及其用于检测分析物的测试元件中的用途。