Methods for isolating nucleic acids
    41.
    发明授权
    Methods for isolating nucleic acids 有权
    分离核酸的方法

    公开(公告)号:US07329491B2

    公开(公告)日:2008-02-12

    申请号:US10962916

    申请日:2004-10-12

    摘要: A method for purification of a nucleic acid comprising the steps of (a) adsorbing on a substrate the nucleic acid from a composition containing (i) an aqueous buffer, (ii) salts in a high concentration, (iii) a water-miscible, non-acidic organic compound, and (iv) the nucleic acid; (b) optionally washing with a washing solution the substrate with the adsorbed nucleic acid; (c) contacting the substrate with the adsorbed nucleic acid with a solution containing salts in a lower concentration compared to the composition of step (a), thereby desorbing the nucleic acid from the substrate; (d) separating the solution with the desorbed nucleic acid from the substrate, thereby purifying the nucleic acid; and optionally (e) precipitating the desorbed nucleic acid from the solution of step (d) and isolating the precipitated nucleic acid, thereby further purifying the nucleic acid.

    摘要翻译: 一种用于纯化核酸的方法,包括以下步骤:(a)从含有(i)水性缓冲液,(ii)高浓度盐,(iii)水混溶性, 非酸性有机化合物,和(iv)核酸; (b)任选地用洗涤溶液洗涤具有吸附的核酸的底物; (c)与步骤(a)的组合物相比,使含有较低浓度盐的溶液与吸附的核酸接触,从而从底物解吸核酸; (d)将解离的核酸与底物分离,从而纯化核酸; 和任选地(e)从步骤(d)的溶液沉淀解吸的核酸并分离沉淀的核酸,由此进一步纯化核酸。

    Labeling reagent
    42.
    发明申请
    Labeling reagent 有权
    标记试剂

    公开(公告)号:US20070196852A1

    公开(公告)日:2007-08-23

    申请号:US11707399

    申请日:2007-02-16

    IPC分类号: C12Q1/68 C07H19/04 C07H21/04

    摘要: The current invention restates substituted indole nucleosides as both terminal as well as internal building blocks of labeled oligonucleotide probes for the detection, analysis and quantitation of nucleic acids. The substituent comprises a linker and a detectable group or a linker and a reactive group for post synthesis coupling. These modified nucleosides grant access to a wide application area. These new substituted indole nucleosides can be used as labeling reagents for the facile preparation of e.g. optimized hybridization probes, Taqman-probes, or molecular beacon probes.

    摘要翻译: 本发明将取代的吲哚核苷作为两个末端以及用于核酸检测,分析和定量的标记的寡核苷酸探针的内部构建块重述。 取代基包括接头和可检测的基团或接头以及用于后合成偶联的反应性基团。 这些修饰的核苷允许进入广泛的应用领域。 这些新的取代的吲哚核苷可以用作标记试剂,用于容易地制备例如 优化的杂交探针,Taqman探针或分子信标探针。

    Methods for isolating nucleic acids
    47.
    发明申请
    Methods for isolating nucleic acids 有权
    分离核酸的方法

    公开(公告)号:US20050079535A1

    公开(公告)日:2005-04-14

    申请号:US10962916

    申请日:2004-10-12

    摘要: A method for purification of a nucleic acid comprising the steps of (a) adsorbing on a substrate the nucleic acid from a composition containing (i) an aqueous buffer, (ii) salts in a high concentration, (iii) a water-miscible, non-acidic organic compound, and (iv) the nucleic acid; ( b) optionally washing with a washing solution the substrate with the adsorbed nucleic acid; (c) contacting the substrate with the adsorbed nucleic acid with a solution containing salts in a lower concentration compared to the composition of step (a), thereby desorbing the nucleic acid from the substrate; (d) separating the solution with the desorbed nucleic acid from the substrate, thereby purifying the nucleic acid; and optionally (e) precipitating the desorbed nucleic acid from the solution of step (d) and isolating the precipitated nucleic acid, thereby further purifying the nucleic acid.

    摘要翻译: 一种用于纯化核酸的方法,包括以下步骤:(a)从含有(i)水性缓冲液,(ii)高浓度盐的组合物,(iii)水混溶性, 非酸性有机化合物,和(iv)核酸; (b)任选地用洗涤溶液洗涤具有吸附的核酸的底物; (c)与步骤(a)的组合物相比,使含有较低浓度盐的溶液与吸附的核酸接触,从而从底物解吸核酸; (d)将解离的核酸与底物分离,从而纯化核酸; 和任选地(e)从步骤(d)的溶液沉淀解吸的核酸并分离沉淀的核酸,由此进一步纯化核酸。

    Device for hot dip coating metal strands
    48.
    发明申请
    Device for hot dip coating metal strands 失效
    热镀金属线束装置

    公开(公告)号:US20050076835A1

    公开(公告)日:2005-04-14

    申请号:US10503871

    申请日:2003-02-20

    CPC分类号: C23C2/24

    摘要: The invention relates to a device for hot dip coating metal strands (1), particularly strip steel, in which the metal strand (1) can be vertically guided through a reservoir (3), which accommodates the molten coating metal (2), and though a guide channel (4) connected upstream therefrom. An electromagnetic inductor (5) is mounted in the area of the guide channel (4) and in order to retain the coating metal (2) inside the reservoir (3), can induce induction currents in the coating metal (2) by means of an electromagnetic traveling field. While interacting with the electromagnetic traveling field, said induction currents exert an electromagnetic force. The inductor (5) has at least two main coils (6) that are arranged in succession in movement direction (X) of the metal strand (1), and has at least two correction coils (7) for controlling the position of the metal strand (1) inside the guide channel (4) in direction (N), which is normal to the surface of the metal strand (1). These correction coils are also arranged in succession in movement direction (X) of the metal strand (1). In order to improve the efficiency of the control of the metal strip inside the guide channel, the invention provides that at least a portion of the correction coils (7), when viewed in movement direction (X) of the metal strand (1), are arranged so that they are offset with regard to one another perpendicular to movement direction (X) and perpendicular to direction (N) that is normal to the surface of the metal strand (1).

    摘要翻译: 本发明涉及一种用于热浸镀金属线(1),特别是带钢的装置,其中金属线(1)可以垂直地引导通过容纳熔融涂层金属(2)的储存器(3),以及 尽管其上游连接有引导通道(4)。 电磁感应器(5)安装在引导通道(4)的区域中,为了将涂层金属(2)保持在储存器(3)内部,可以通过以下方式引起涂层金属(2)中的感应电流: 电磁行驶场。 当与电磁行进场相互作用时,所述感应电流施加电磁力。 电感器(5)具有至少两个主要线圈(6),它们沿金属线束(1)的移动方向(X)连续布置,并且具有至少两个用于控制金属线的位置的校正线圈(7) 在导向通道(4)内的方向(N)上的线(1),其垂直于金属线(1)的表面。 这些校正线圈也在金属线(1)的移动方向(X)上相继排列。 为了提高引导通道内的金属带的控制效率,本发明提供了当从金属线(1)的移动方向(X)观察时,至少一部分校正线圈(7) 被布置成使得它们相对于垂直于运动方向(X)并且垂直于垂直于金属线(1)的表面的方向(N)彼此偏移。

    Aerobically curable adhesive
    50.
    发明授权
    Aerobically curable adhesive 失效
    有氧可固化胶粘剂

    公开(公告)号:US6096842A

    公开(公告)日:2000-08-01

    申请号:US875394

    申请日:1997-07-24

    IPC分类号: C09J4/00 C09J4/02 C09J4/06

    CPC分类号: C09J4/00 C09J4/06

    摘要: Aerobically hardening adhesives based on polymers of acrylates or methacrylates having a relatively high boiling point such as polyurethane (meth)acrylates are described, wherein the adhesives contain at least one initiator capable of forming free radicals, at least one accelerator, and at least one siccative. Such adhesives are largely odorless and harden completely such that the adhesive surface at the boundary with air is not tacky.

    摘要翻译: PCT No.PCT / EP96 / 00199 Sec。 371日期1997年7月24日 102(e)1997年7月24日PCT PCT 1996年1月18日PCT公布。 公开号WO96 / 23036 日本1996年8月1日描述了基于具有较高沸点的丙烯酸酯或甲基丙烯酸酯如聚氨酯(甲基)丙烯酸酯的聚合物的自动硬化粘合剂,其中粘合剂含有至少一种能形成自由基的引发剂,至少一种促进剂, 和至少一个siccative。 这种粘合剂大部分无臭并且完全硬化,使得与空气边界处的粘合剂表面不发粘。