公开(公告)号：US20090239254A1

公开(公告)日：2009-09-24

申请号：US12382384

申请日：2009-03-16

申请人： Christine DUVAL ,  Francoise BERNERD

发明人： Christine DUVAL ,  Francoise BERNERD

IPC分类号： C12Q1/02 ,  C12N5/08

CPC分类号： C12N5/0698 ,  C12N2501/11 ,  C12N2501/117 ,  C12N2501/39 ,  C12N2502/091 ,  C12N2502/094 ,  C12N2502/1323 ,  C12N2533/50 ,  C12N2533/52 ,  C12N2533/54 ,  G01N33/5088

摘要： An in vitro skin equivalent includes at least one epidermis equivalent and at least one dermis equivalent, and further includes melanocytes constitutively producing melanin and fibroblasts.

摘要翻译： 体外皮肤当量包括至少一种表皮等同物和至少一种真皮等效物，并且还包括组成型产生黑色素和成纤维细胞的黑素细胞。

公开(公告)号：US07371576B2

公开(公告)日：2008-05-13

申请号：US10658437

申请日：2003-09-08

申请人： Wen-Ghih Tsang ,  Tianli Zheng ,  Wei Liu

发明人： Wen-Ghih Tsang ,  Tianli Zheng ,  Wei Liu

IPC分类号： C12N5/02 ,  C12N5/08

CPC分类号： C12N5/0678 ,  A61K35/12 ,  C12N2500/34 ,  C12N2501/117 ,  C12N2501/12 ,  C12N2501/599 ,  C12N2533/54

摘要： The invention relates to the discovery of a selective cell surface marker that permits the selection of a unique subset of pancreatic stems cells having a high propensity to differentiate into insulin producing cells or into insulin producing cell aggregates.

摘要翻译： 本发明涉及选择性细胞表面标志物的发现，其允许选择具有高分化为胰岛素产生细胞或产生胰岛素的细胞聚集体的高倾向的胰腺干细胞的唯一子集。

公开(公告)号：US20070231897A1

公开(公告)日：2007-10-04

申请号：US11628202

申请日：2005-05-26

申请人： Susumu Seino ,  Kohtaro Minami ,  Masaaki Okuno ,  Kazumasa Miyawaki

发明人： Susumu Seino ,  Kohtaro Minami ,  Masaaki Okuno ,  Kazumasa Miyawaki

IPC分类号： C12N5/08 ,  C12N5/02

CPC分类号： C12N5/0676 ,  C12N2501/11 ,  C12N2501/117 ,  C12N2501/119 ,  C12N2501/12

摘要： Disclosed is a method for producing insulin secreting cells to be used in cell transplantation therapy of diabetes, by induction from pancreatic acinar cells in culture vessels. The method comprises culturing pancreatic acinar cells isolated from a mammal in a medium containing a growth factor, preferably epidermal growth factor, fibroblast growth factor and/or hepatocyte growth factor, to induce insulin secreting cells.

摘要翻译： 公开了通过从培养容器中的胰腺腺泡细胞诱导，用于产生用于糖尿病的细胞移植治疗的胰岛素分泌细胞的方法。 该方法包括在含有生长因子，优选表皮生长因子，成纤维细胞生长因子和/或肝细胞生长因子的培养基中培养从哺乳动物分离的胰腺腺泡细胞，以诱导胰岛素分泌细胞。

公开(公告)号：US20050233447A1

公开(公告)日：2005-10-20

申请号：US11137441

申请日：2005-05-26

申请人： Bernd Karl Kremer ,  Fred Fandrich ,  Maren nee Ruhnke

发明人： Bernd Karl Kremer ,  Fred Fandrich ,  Maren nee Ruhnke

IPC分类号： A61K35/12 ,  A61K35/28 ,  A61L27/00 ,  A61L27/38 ,  A61P1/16 ,  A61P1/18 ,  A61P5/00 ,  A61P9/10 ,  A61P11/00 ,  A61P13/12 ,  A61P17/00 ,  A61P25/00 ,  C12N5/071 ,  C12N5/074 ,  C12N5/077 ,  C12N5/0789 ,  C12N5/079 ,  C12N5/0793 ,  C12N5/08

CPC分类号： A61L27/3886 ,  A61K35/12 ,  A61K2035/124 ,  A61L27/3804 ,  A61L27/3834 ,  A61L27/3895 ,  C12N5/0607 ,  C12N5/0618 ,  C12N5/0619 ,  C12N5/0622 ,  C12N5/0629 ,  C12N5/0647 ,  C12N5/0653 ,  C12N5/067 ,  C12N5/0676 ,  C12N5/069 ,  C12N2500/30 ,  C12N2500/44 ,  C12N2501/10 ,  C12N2501/11 ,  C12N2501/117 ,  C12N2501/119 ,  C12N2501/12 ,  C12N2501/20 ,  C12N2501/22 ,  C12N2501/23 ,  C12N2501/235 ,  C12N2501/33 ,  C12N2501/385 ,  C12N2501/39 ,  C12N2502/14 ,  C12N2506/03 ,  C12N2506/11 ,  C12N2510/00

摘要： The invention relates to the production of adult dedifferentiated, programmable stem cells from human monocytes by cultivation of monocytes in a culture medium which contains M-CSF and IL-3. The invention further relates to pharmaceutical preparations, which contain the dedifferentiated, programmable stem cells and the use of these stem cells for the production of target cells and target tissue.

摘要翻译： 本发明涉及通过在含有M-CSF和IL-3的培养基中培养单核细胞从人单核细胞生产成人去分化的可编程干细胞。 本发明还涉及含有去分化的可编程干细胞的药物制剂，以及这些干细胞用于产生靶细胞和靶组织的用途。

公开(公告)号：US06815203B1

公开(公告)日：2004-11-09

申请号：US09602508

申请日：2000-06-23

申请人： Susan Bonner-Weir ,  Monica Taneja

发明人： Susan Bonner-Weir ,  Monica Taneja

IPC分类号： C12N506

CPC分类号： C12N5/0676 ,  A61K35/12 ,  C12N2500/90 ,  C12N2501/117 ,  C12N2506/22

摘要： The invention features methods of promoting dedifferentiation of pancreatic cells, methods of obtaining pancreatic islet cells from the dedifferentiated pancreatic cells, and methods of treating a subject having a disorder characterized by insufficient pancreatic islet function by administering pancreatic islet cells obtained by these methods.

摘要翻译： 本发明的特征在于促进胰细胞去分化的方法，从去分化胰腺细胞获得胰岛细胞的方法以及通过施用通过这些方法获得的胰岛细胞来治疗具有不足胰岛功能的病症的受试者的方法。

公开(公告)号：US20040132183A1

公开(公告)日：2004-07-08

申请号：US10447325

申请日：2003-05-28

发明人： David William Scharp ,  Paul Presley Latta ,  Margaret Coutts ,  Catherine Anne McIntyre

IPC分类号： C12N005/08

CPC分类号： C12N5/0676 ,  A61K35/12 ,  A61K2035/126 ,  C12N5/0037 ,  C12N2500/25 ,  C12N2500/38 ,  C12N2500/46 ,  C12N2501/01 ,  C12N2501/105 ,  C12N2501/11 ,  C12N2501/113 ,  C12N2501/115 ,  C12N2501/117 ,  C12N2501/12 ,  C12N2501/135 ,  C12N2501/15 ,  C12N2501/16 ,  C12N2501/165 ,  C12N2501/235 ,  C12N2501/315 ,  C12N2501/335 ,  C12N2501/34 ,  C12N2501/345 ,  C12N2501/35 ,  C12N2501/37 ,  C12N2501/39 ,  C12N2501/392 ,  C12N2501/41 ,  C12N2501/83 ,  C12N2501/85 ,  C12N2501/998 ,  C12N2506/22 ,  Y02A50/471

摘要： A method of converting differentiated non-hormone producing pancreatic cells into differentiated hormone producing cells is disclosed. The method comprises two steps: first, culturing cells under conditions which convert differentiated non-hormone producing cells into stem cells; and second, culturing stem cells under conditions which provide for differentiating stem cells into hormone-producing cells. The invention provides a new source of large quantities of hormone producing cells such as insulin-producing cells that are not currently available for therapeutic uses such as the treatment of diabetes.

摘要翻译： 公开了将分化的非激素产生胰腺细胞转化成分化的激素产生细胞的方法。 该方法包括两个步骤：首先在将分化的非激素产生细胞转化为干细胞的条件下培养细胞; 其次，在将干细胞分化成激素产生细胞的条件下培养干细胞。 本发明提供了大量激素产生细胞的新来源，例如目前不能用于诸如治疗糖尿病的治疗用途的胰岛素产生细胞。

公开(公告)号：US20010013134A1

公开(公告)日：2001-08-09

申请号：US09826779

申请日：2001-04-05

发明人： Nora Sarvetnick ,  Michelle L. Krakowski ,  Marcie R. Kritzik

IPC分类号： A01K067/027 ,  A61K048/00 ,  A61K039/395

CPC分类号： C12N15/8509 ,  A01K67/0271 ,  A01K67/0275 ,  A01K2217/05 ,  A01K2227/105 ,  A01K2267/03 ,  A01K2267/0325 ,  A61K38/1808 ,  A61K38/1825 ,  A61K39/00 ,  A61K48/00 ,  C07K16/18 ,  C07K2319/30 ,  C12N5/0672 ,  C12N5/0676 ,  C12N5/0678 ,  C12N2501/11 ,  C12N2501/117 ,  C12N2503/00 ,  C12N2517/02 ,  C12N2830/008 ,  G01N33/5005 ,  G01N33/566

摘要： The invention provides animal models, where the ectopic expression of KGF, EGF, or both is under the control of a pancreas-specific promoter, e.g., the insulin promoter. The expression of KGF in the ins-KGF pancreatic islets of Langerhans results in enlarged islets, with substantial proliferation of duct cells within the islet mass, and the presence of albumin and alpha-fetoprotein-producing hepatocytes in the islets of the ins-KGF pancreata. The compositions and methods disclosed are useful for identifying and isolating pancreatic stem/progenitor cells, including a common stem/progenitor to liver cells and pancreatic cells.

公开(公告)号：US12049646B2

公开(公告)日：2024-07-30

申请号：US18395362

申请日：2023-12-22

申请人： President and Fellows of Harvard College

发明人： Quinn P. Peterson ,  Felicia J. Pagliuca ,  Douglas A. Melton ,  Jeffrey Robert Millman ,  Michael Saris Segel ,  Mads Gurtler

IPC分类号： C12N5/071 ,  A61K35/39 ,  A61P3/10 ,  A61P5/48 ,  A61P5/50 ,  C12N5/0735 ,  C12N5/074

CPC分类号： C12N5/0678 ,  A61K35/39 ,  A61P3/10 ,  A61P5/48 ,  A61P5/50 ,  C12N5/0606 ,  C12N5/0676 ,  C12N5/0696 ,  C12N2501/11 ,  C12N2501/117 ,  C12N2501/15 ,  C12N2501/16 ,  C12N2501/375 ,  C12N2501/385 ,  C12N2501/395 ,  C12N2501/40 ,  C12N2501/41 ,  C12N2501/727 ,  C12N2501/998 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2506/03 ,  C12N2506/22 ,  C12N2506/45

摘要： Disclosed herein are methods, compositions, kits, and agents useful for inducing β cell maturation, and isolated populations of SC-β cells for use in various applications, such as cell therapy.

公开(公告)号：US20240240154A1

公开(公告)日：2024-07-18

申请号：US18473619

申请日：2023-09-25

申请人： Children's Hospital Medical Center

发明人： James M. Wells ,  Jorge Orlando Munera

IPC分类号： C12N5/071

CPC分类号： C12N5/0679 ,  C12N2501/11 ,  C12N2501/113 ,  C12N2501/115 ,  C12N2501/117 ,  C12N2501/119 ,  C12N2501/155 ,  C12N2501/415 ,  C12N2506/02

摘要： Disclosed herein are methods for the in vitro differentiation of a precursor cell into definitive endoderm, which may further be differentiated into a human colonic organoid (HCO), via modulation of signaling pathways. Further disclosed are HCOs and methods of using HCOs, which may be used, for example, for the HCOs may be used to determine the efficacy and/or toxicity of a potential therapeutic agent for a disease selected from colitis, colon cancer, polyposis syndromes, and/or irritable bowel syndrome.

公开(公告)号：US11905530B2

公开(公告)日：2024-02-20

申请号：US17127221

申请日：2020-12-18

申请人： ViaCyte, Inc.

发明人： Alan D. Agulnick ,  Olivia Kelly ,  Yuki Ohi ,  Allan Robins ,  Thomas Schulz

IPC分类号： C12N5/071 ,  A61K35/39

CPC分类号： C12N5/0676 ,  A61K35/39 ,  C12N2500/25 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/117 ,  C12N2501/16 ,  C12N2501/195 ,  C12N2501/385 ,  C12N2501/41 ,  C12N2501/415 ,  C12N2501/727 ,  C12N2506/02 ,  C12N2506/45

摘要： Disclosed herein are cell culture compositions, for example, pancreatic cell culture compositions, derived from dedifferentiated human reprogrammed pluripotent stem cells, such as induced pluripotent stem (iPS) cells, and methods for producing and using such cell culture compositions.